• 한국연초학회지
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• 제18권2호
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• pp.101-106
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• 1996

Tobacco mosaic virus (TMV) tomato strain was isolated from tomato "Seo-Kwang" in Korea. The virion was purified by density gradient centrifugation, and total viral RNA was isolated from the purified particles. Coat protein (CP) cDNA of the virus was synthesized by RT-PCR, and the purified cDNA fragment was subcloned to pBluescript II SK-. The analysis of nucleotide sequence showed that this cDNA was 693 nucleotides long from the insert of clone p1571 and p1572 which contain complete codons of the viral coat protein gene (474 nucleotides) and 3' untranslated region. The nucleotides of coat protein encoding cDNA of the strain were 6 nucleotides less than that of TMV common strain isolated from tobacco plant in Korea. The CP gene showed 70% maximum homology with that of the common strain in the nucleotide level and 86% maximum homology in amino acid level.cid level.

• 한국미생물·생명공학회지
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• 제25권6호
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• pp.580-585
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• 1997

We selected two mutants namely strain D5 and Nu23 by mutagenesis of anthracycline producing Streptomyces: the former is an $\varepsilon$-rhodomycinone overproducing mutant selected from Streptomyces sp. C5, a baumycin producer and the latter, a blocked mutant of early pathway for doxorubicin biosynthesis obtained from Streptomyces peucetius ATCC 27952, a doxorubicin producer. The mutant strain Nu23 does not produce anthracycline metabolites but retains the most of enzyme activities converting aklavinone to doxorubicin and the mutant strain D5 produced $\varepsilon$-rhodomycinone at a level of 150 $\mu$g/ml. These strains were grown separately in NDYE medium and each was mixed at day 3 by equal volume of culture broth but the quantity of doxorubicin produced was far below an estimation based on the level of $\varepsilon$-rhodomycinone normaly produced by the strain D5. On the other hand doxorubicin was reached at maximum level after 4 days in the mixed culture condition which was composed of culture broth of strain D5 grown for 6 day and that of strain Nu23 grown for 3 day. It was turned out that the growth of mutant strain D5 was inhibited by the accumulation of daunorubicin and doxorubicin in mixed culture broth, which cause the limitation of $\varepsilon$-rhodomycinone.

• Geomechanics and Engineering
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• 제21권4호
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• pp.327-336
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• 2020

Conventional researches on the behavior of fiber-reinforced and unreinforced soils often investigated the failure point. In this study, a concept is proposed in the comparison of the fiber-reinforced with unreinforced sand, by estimating the strength and strength ratio at different levels of strain. A comprehensive program of laboratory drained triaxial compression test was performed on compacted sand specimens, with and without date palm fiber. The fiber inclusion used in triaxial test specimens was form 0.25%-1.0% of the sand dry weight. The effect of the fiber inclusion and confining pressure at 0.5%, 1.0%, 1.5%, 3.0%, 6.0%, 9.0%, 12%, and 15% of the imposed strain levels on the specimen were considered and described. The results showed that, the trend and magnitude of the strength ratio is different for various strain levels. It also implies that, using failure strength from peak point or the strength corresponding to the axial strain of approximately 15% for evaluating the enhancement of strength or strength ratio, due to the reinforcement, may cause hazard and uncertainty in practical design. Therefore, it is necessary to consider the strength of fiber-reinforced specimen at the imposed strain level, compared to the unreinforced specimen.

• 한국운동역학회지
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• 제15권1호
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• pp.155-176
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• 2005

There is some evidence that one of major factors to produce plantar fasciitis depends on the magnitude of the foot arch strain. The orthotics that can reduce the foot arch strain during locomotion may be effective to prevent or treat plantar fasciitis. Therefore, the purpose of this study was to investigate the effect of control condition and three types of foot orthotics on 3-dimensional foot arch strain that can produce plantar fasciitis during treadmill level and uphill walking and running. Sixteen male subjects are recruited and the arch length and height strain according to three types of foot orthotics with respect to control condition were measured by using two digital video cameras. The first hypothesis which the comfort of foot orthotics would be increased from arch pad, half length orthotics to full length orthotics was mostly accepted. It suggested that the types of the foot orthotics could be properly prescribed according foot regions that is pain or abnormal. The second hypothesis which the foot arch strain can be reduced by foot orthotics during level heel-toe walking and running and the third hypothesis which the foot arch strain can be reduced by foot orthotics during uphill heel-toe walking and running were rejected. The foot arch length and height strain during walking and running showed small and subject-specific characteristics and could not be optimal biomechanical variable to prove the overall comfort. The forth hypothesis which the foot arch strain cannot be reduced by foot orthotics during uphill toe walking and running was accepted. With the foot arch length and height strain during uphill toe walking and running the windlass mechanism suggested by Hicks can be explained successfully and excessive uphill toe walking and running can be one of cause of plantar fasciitis. The dynamic investigation on the foot arch such as walking and running should be carefully observed with integrated insights considering ligaments and foot bones as well as plantar fascia, extrinsic muscles and tendons, and intrinsic muscles and tendons.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2001년도 춘계학술대회 논문집
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• pp.249-256
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• 2001

This study investigated the shear stiffness of level of small strain with time effect. Time effect consists of rest time, loading rate of recent and current stress path. In addition, for the measurement of small strain, overconsolidated state was represented in a triaxial cell, and drained stress path tests were carried out. Test results show that the loading rate of recent stress path has no effects on the stiffness of very small strain, but the shear stiffness of level of small strain increases with it. Finally, the rest time and the loading rate of current stress path have the effects on the shear stiffness of initial and small strain.

• 소성∙가공
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• 제28권3호
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• pp.154-158
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• 2019

The hole expansion tests using conical punch, flat punch or hemispherical punch are widely used for stretch flangeability verification of HSS. In this study, we investigate the strain distribution on the shear edges of the hole expansion test using grid marking and a projector. A small crack at the edge is distributed, resulting in a large gap between the HER and the crack strain. The strain distribution at the edges is irregular due to anisotropy of sheet metal. While an edge perpendicular to the rolling direction indicate a lower strain level compared to an edge parallel to the rolling direction, edge cracks occur at the edge perpendicular to the rolling direction. To predict the manifestation of edge cracks in FE analysis, the result of the hole expansion test with a crack strain measurement may well be a better tool than FLD. In this case, the level of strain and the direction of the edge relative to the rolling direction should be well considered.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2008년도 춘계 학술발표회 초청강연 및 논문집
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• pp.90-96
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• 2008

Engineered mixtures composed of rigid sand particles and soft rubber particles are tested to investigate their behavior with strain level. Mixtures are prepared with different volumetric sand fractions (sf) to identify response using small strain resonant column, intermediate strain oedometer, and large strain direct shear tests. The small strain shear modulus and damping ratio are determined with volumetric sand fractions. The asymmetric frequency response curve increases with decreasing sand fraction. Linear responses of shear strain and damping ratio with shear strain are observed at the mixture of sf=0.2. Vertical strain increases with decreasing sand fraction. Mixtures with $04.{\leq}sf{\leq}0.6$ show the transitional stress-deformation behavior from rubber-like to sand-like behavior. The friction angle increases with the sand fraction and no apparent peak strength is observed in mixture without sf=1.0.

• Structural Engineering and Mechanics
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• 제11권5호
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• pp.565-574
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• 2001

Experimental investigation into the behaviour of half-scale brick masonry panels were conducted under cyclic loading normal to the bed joint and parallel to the bed joint. For each cycle, full reloading was performed with the cycle peaks coinciding approximately with the envelope curve. Unloading, however, was carried out fully to zero stress level and partially to two different stress levels of 25 percent and 50 percent of peak stress. Stability point limit exhibits a unique stress-strain curve for full unloading but it could not be established for partial unloading. Common point limit was established for all unloading-reloading patterns considered, but its location depends on the stress level at which unloading is carried to. Common point curves were found to follow an exponential formula, while residual strains versus envelope strains can be expressed by a polynomial function of a single term. The relation between residual strain and envelope strain can be used to determine the stress level at which deterioration due to cyclic loading began.

• Biomolecules & Therapeutics
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• 제1권1호
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• pp.26-30
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• 1993

Strain of treptomyces minoensis DMCJ-144 was tried to be improved so that it produces much more the $\alpha$-amylase inhibitor. Streptomyces minoensis DMCJ-144 was treated with 1 mg/mι (pH 9.0) of N-methyl-N'-nitro-N-nitrosoguanidine at $30^{\circ}C$ for 60 min and irradiated with UV light distanced 30 cm for 20 min. After mutagenesis, surviving colonies were cultured on the CM contaning acriflavine ($10{\mu}g/ml$) three times in order to enhance the mutability. And then through multi-level screening, colonies that ${\alpha}$-amylase inhibitor productibility. was Improved were selected by modified-blue value method. After third acriflavine treatment, $\alpha$-amylase inhibitory activities of selected colonies were found to be much better as compared with that of parent strain. One mutant strain showed 5.4 time inhibitory activity than the parent strain.

• 대한기계학회논문집A
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• 제25권8호
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• pp.1220-1226
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• 2001

This study investigates the effects of the pre-strain level on mechanical properties of the solid-phase formed thermoplastic composite. A uniaxial solid-phase forming was performed at the temperature of 125$\^{C}$ and at the constant cross-head speed of 3mm/sec. The composite sheet was formed to various pre-strain levels of 10%, 20%, and 30%. Tension, flexural, and impact tests were carried out to characterize the material properties of a solid-phase formed part. Tensile and flexural strengths decreased with increasing the pre-strain level, while impact strength increased. Various microstructures of the formed part explained the above material behavior.