• Title/Summary/Keyword: Strain difference

Search Result 1,110, Processing Time 0.027 seconds

Biofilm Formation and Low pH Viability of Cronobacter spp. (Enterobacter sakazakii) Isolated from Powdered Infant Formula and Infant Foods in Korea (국내 분유 및 영.유아식품에서 분리된 Cronobacter spp. (Enterobacter sakazakii)의 Biofilm 생성 특성 및 내산성 비교)

  • Kim, Sun-Ae;Lee, Yu-Mi;Oh, Se-Wook;Gwak, Hyo-Sun;Hwang, In-Gyun;Kang, Dong-Hyun;Woo, Gun-Jo;Rhee, Min-Suk
    • Food Science of Animal Resources
    • /
    • v.29 no.6
    • /
    • pp.702-708
    • /
    • 2009
  • We investigated biofilm formation in various media, growth in low pH, and the hemolytic activity of 14 strains of Cronobacter spp. (Enterobacter sakazakii) isolated from a variety of foods including powdered infant formula (n=75), infant cereal (n=100), honey (n=30), and other infant foods (n=100) in Korea. The Cronobacter spp. adhered and formed biofilms on polyethylene, and a greater extent of biofilm was observed in nutrient-rich media. No clear difference in biofilm-forming ability was noted among the media constituents and the pattern of biofilm formation was strain-dependent. Seven strains out of 14 strains (50%) grew at pH 4.1, indicating that the acid resistance of these Cronobacter spp. isolated in Korea was relatively low. Hemolytic activity was not observed in any of the strains. This study provides basic information for the physiological and biochemical characteristics of Cronobacter spp. isolated from a variety of infant foods in Korea.

Follower Effect of the Axisymmetric Shells under External Pressure (축대칭 쉘 구조물에 작용하는 외압의 부가효과)

  • Hwang, Chul-Sung
    • Journal of the Korea institute for structural maintenance and inspection
    • /
    • v.8 no.1
    • /
    • pp.195-202
    • /
    • 2004
  • The shell due to the effect of initial normal pressures on the shell surface was based on the assumption that the directions of the pressures are always normal to the undeformed shell surface, and that the change in the surface area of the shell is negligible. But the fact that the pressure are always normal to the deforming surface leads "follower force". The follower effect in the analysis can significantly alter the solution for natural frequency and buckling load as compared to the case when the direction of the pressures are assumed to be normal to the uniform shell surface. The expression for the part of strain energy contribution from normal pressure due to the effect of follower force was derived and added to the element stiffness matrix of axisymmetric shell. In the case of increasing external pressure, the natural frequencies decrease until one of them reaches zero. Theoretically the smallest applied load that reduces the frequency of any mode to zero, will have same magnitude as that of the buckling load. In order to determine the bucking load of the shell a few sets of frequencies are computed and the results considering the follower effects are well with the exact solution while the case without that are quite different. But in case of hemispherical dome, there are little difference in buckling pressure between with and without the effect of follower force.

Risk Factors for the Treatment Failure of Antibiotic-Loaded Cement Spacer Insertion in Diabetic Foot Infection (당뇨병성 족부 감염에서 항생제 혼합 시멘트 충전물 사용의 치료 실패 위험 인자 분석)

  • Park, Se-Jin;Song, Seungcheol
    • Journal of Korean Foot and Ankle Society
    • /
    • v.23 no.2
    • /
    • pp.58-66
    • /
    • 2019
  • Purpose: To evaluate the efficacy of antibiotic-loaded cement spacers (ALCSs) for the treatment of diabetic foot infections with osteomyelitis as a salvage procedure and to analyze the risk factors of treatment failure. Materials and Methods: This study reviewed retrospectively 39 cases of diabetic foot infections with osteomyelitis who underwent surgical treatment from 2009 to 2017. The mean age and follow-up period were $62{\pm}13years$ and $19.2{\pm}23.3months$, respectively. Wounds were graded using the Wagner and Strauss classification. X-ray, magnetic resonance imaging (or bone scan) and deep tissue cultures were taken preoperatively to diagnose osteomyelitis. The ankle-brachial index, toe-brachial index (TBI), and current perception threshold were checked. Lower extremity angiography was performed and if necessary, percutaneous transluminal angioplasty was conducted preoperatively. As a surgical treatment, meticulous debridement, bone curettage, and ALCS placement were employed in all cases. Between six and eight weeks after surgery, ALCS removal and autogenous iliac bone graft were performed. The treatment was considered successful if the wounds had healed completely within three months without signs of infection and no additional amputation within six months. Results: The treatment success rate was 82.1% (n=32); 12.8% (n=5) required additional amputation and 5.1% (n=2) showed delayed wound healing. Bacterial growth was confirmed in 82.1% (n=32) with methicillin-resistant Staphylococcus aureus being the most commonly identified strain (23.1%, n=9). The lesions were divided anatomically into four groups; the largest number was the toes: (1) toes (41.0%, n=16), (2) metatarsals (35.9%, n=14), (3) midfoot (5.1%, n=2), and (4) hindfoot (17.9%, n=7). A significant difference in the Strauss wound score and TBI was observed between the treatment success group and failure group. Conclusion: The insertion of ALCSs can be a useful treatment option in diabetic foot infections with osteomyelitis. Low scores in the Strauss classification and low TBI are risk factors of treatment failure.

Implementation of PTT Change Monitoring System According to Exercise Intensity (PTT기반 운동 강도 모니터링 시스템 구현)

  • Lee, Ji-Su;Kim, Dong-Chan;Lee, Gyeong-Tack;Kim, Gyeong-Seop;Noh, Yun-Hong;Jeong, Do-Un
    • Journal of the Institute of Convergence Signal Processing
    • /
    • v.21 no.1
    • /
    • pp.49-54
    • /
    • 2020
  • Cardiovascular disease is the leading cause of death worldwide and is caused by a variety of causes. The highest risk factor for cardiovascular disease is high blood pressure, which has no obvious symptoms, but if left untreated, it causes several complications. In order to treat hypertension, medication and regular exercise are required. In people with high blood pressure, excessive physical activity can put a great strain on the heart and lead to cardiovascular disease. Therefore, there is a need for an exercise intensity monitoring system through PTT measurement that can perform exercise at an appropriate intensity. In this study, we implemented a PTT change monitoring system according to exercise intensity by calculating PTT through ECG and PPG measurement. The implemented system differentiates the R-peak of the ECG and P-peak of the PPG, and calculates the PTT using the time difference between R-peak and P-peak. A running experiment was conducted to monitoring PTT change according to exercise intensity. As a result of the experiment, low intensity PTT is 0.313s, moderate is 0.220s, high is 0.188s, it was confirmed that the PTT decreased as the exercise increase increased.

A Study on the Buckling Stability due to Lateral Impact of Gas Pipe Installed on the Sea-bed (해저면에 설치된 가스관의 외부충격에 의한 좌굴 안전성 검토)

  • Park, Joo-Shin;Yi, Myung-Su
    • Journal of the Korean Society of Marine Environment & Safety
    • /
    • v.28 no.2
    • /
    • pp.414-421
    • /
    • 2022
  • Subsea oil and gas exploration is increasingly moving into deeper water depths, and typically, subsea pipelines operate under high pressure and temperature conditions. Owing to the difference in these components, the axial force in the pipe is accumulated. When a pipeline is operated at a high internal pressure and temperature, it will attempt to expand and contract for differential temperature changes. Typically, the line is not free to move because of the plane strain constraints in the longitudinal direction and soil friction effects. For a positive differential temperature, it will be subjected to an axial compressive load, and when this load reaches a certain critical value, the pipe may experience vertical (upheaval buckling) or lateral (snaking buckling) movements that can jeopardize the structural integrity of the pipeline. In these circumstances, the pipeline behavior should be evaluated to ensure the pipeline structural integrity during operation in those demanding loading conditions. Performing this analysis, the correct mitigation measures for thermal buckling can be considered either by accepting bar buckling but preventing the development of excessive bending moment or by preventing any occurrence of bending.

Structural Performance Evaluation of a Multi-span Greenhouse with Venlo-type Roof According to Bracing Installation (가새 설치에 따른 벤로형 지붕 연동온실의 구조성능 평가)

  • Shin, Hyun Ho;Choi, Man Kwon;Cho, Myeong Whan;Kim, Jin Hyun;Seo, Tae Cheol;Lee, Choung Kuen;Kim, Seung Yu
    • Journal of Bio-Environment Control
    • /
    • v.31 no.4
    • /
    • pp.438-443
    • /
    • 2022
  • In this study, the lateral loading test was performed to analyze structural performance of multi-span plastic greenhouse through full-scale experiment and numerical analysis. In order to analyze the lateral stiffness and stress, we installed 9 displacement sensors and 19 strain gauge sensors on the specimen, respectively, and load of l mm per minute was applied until the specimen failure. In the comparison between the full-scale experiment and the structural analysis results of a multi-span greenhouse with venlo-type roof according to bracing installation, there was a large difference in the lateral stiffness of the structure. By installing a brace system, the lateral stiffness measured near the side elevation of the specimen increased by up 44%. As the bracing joint used in the field did not secure sufficient rigidity, the external force could not be transmitted to the entire structure properly. Therefore, it is necessary to establish a bracing construction method and design standards in order for a greenhouse to which bracing applied to have sufficient performance.

Evaluation of Strength and Deformability of a Friction Material Based on True Triaxial Compression Tests (진삼축압축시험을 통한 마찰재료의 강도 및 변형 특성 평가)

  • Bae, Junbong;Um, Jeong-Gi;Jeong, Hoyoung
    • The Journal of Engineering Geology
    • /
    • v.32 no.4
    • /
    • pp.597-610
    • /
    • 2022
  • Knowledge of the failure behavior of friction materials considering their intermediate principal stress is related to an understanding of situations where these materials might be used: for example, the stability of deep-seated boreholes and fault slip analysis. This study designed equipment for physically implementing true triaxial compression and used it to assess specimens of plaster, a friction material. The material's mechanical behaviors are discussed based on the results. The applicability of the 3D failure criteria are also reviewed. The tested specimens were molded cuboids of width, length, and height 52, 52, and 104 mm, respectively. A total of 24 true triaxial compression tests were performed under various combinations of 𝜎3 and 𝜎2 conditions. Conventional uniaxial and triaxial compression tests were employed to estimate the mechanical properties of the plaster for use as parameters for 3D failure criteria. Examining the stress-strain relations of the plaster materials showed that a large difference between the intermediate principal stress and the minimum principal stress indicated strong brittle behavior. The mechanical behavior of the plaster used here reflects the change of intermediate principal stress. Nonlinear multiple regression analysis on the test data in the principal space showed that the modified Wiebols-Cook failure criterion and the modified Lade failure criterion were the most suitable 3D failure criteria for the tested plaster.

Introduction, Development, and Characterization of Supernodulating Soybean Mutant. 1. Mutagenesis of Soybean and Selection of Supernodulating Soybean Mutant (다량 뿌리혹 형성 콩 계통의 도입 개발 및 생육특성구명 1. 돌연변이유기에 의한 콩 초다뿌리혹형성 계통선발)

  • Lee, Hong-Suk;Chae, Young-Am;Park, Eui-Ho;Kim, Yong-Wook;Yun, Kwang-Il;Lee, Suk-Ha
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.42 no.2
    • /
    • pp.247-253
    • /
    • 1997
  • Development of soybean cultivars with great nodulation and high nitrogen fixation activity, derived mostly from mutagenesis, may decrease inputs of chemical fertilizer nitrogen into the soil-plant system. Soybean seeds (cv. Jangyupkong, Hwanggeumkong, and Geomjungkong 1) were treated with three different levels of EMS (ethyl methanesulfonate) concentration(30, 50, and 70mM). Increasing the doses of EMS resulted in decreased field emergence rate of seeds, whereas it did not increase M$_2$ mutation frequencies. This indicated that the most efficient concentration of EMS was 30mM for generating mutants. Extensive mutagenesis of Sinpaldalkong 2 with 30mM EMS was undertaken to isolate soybean mutants with greater nodulation. Approximately 8, 200 M$_2$ families were screened for greater nodulation on 5 mM nitrate after inoculation with Bradyrhizobium japonicum strain YCK213-KFCC-10728. Mutant SS-2 nodulated more than the wild type. Comparison of supernodulation between SS-2 and two nts mutants(nts 1007 and nts 1116) revealed that SS-2 showed the supernodulation character at an earlier growth stage than the two nts mutants. Further studies should be needed to characterize the difference in timing of nodulation between SS-2 and nts mutants.

  • PDF

The Effect of Pile Distallation on the Reduction of Cumulative Plastic Settlement (말뚝 설치를 통한 콘크리트궤도의 누적소성침하 감소 효과)

  • Lee, Su-Hyung;Lee, Il-Wha;Lee, Sung-Jin;Kim, Dae-Sang
    • Journal of the Korean Geotechnical Society
    • /
    • v.24 no.5
    • /
    • pp.129-137
    • /
    • 2008
  • An active application of concrete track is being expected far the future constructions of Korean railroad. In comparison with the existing ballasted tract, a concrete track is very susceptible for the settlement, since its rehabilitation requires much time and cost. When a concrete track is constructed on fine-grained subgrade soil, excessive cumulative plastic settlements due to repetitive train road may occur. In this case, the settlement of the concrete track may be effectively reduced by installing a small number of small-diameter piles beneath the track. This paper presents the effect of pile installation on the reduction of cumulative plastic settlement of concrete track. A method combining experiential equation and numerical method is proposed. Using an existing experiential equation and the estimated earth pressure distribution, the cumulative plastic strain was calculated. From the results, it is verified that the effects of the pile installation is significant to effectively reduce the cumulative plastic settlement of concrete track. The reduction effects of the cumulative plastic settlement according to the pile number and pile arrangement are presented.

Study of the Effects of Sodium Chloride on Segregation-Distorter Action in D. melanogaster: 1. Theatment of Sodium Chloride on Whole Developmental Stages.

  • Kang, M.J.;Chung, Y.J.;Kim, K.J.
    • The Korean Journal of Zoology
    • /
    • v.13 no.1
    • /
    • pp.21-25
    • /
    • 1970
  • The present paper deals with the results of the experiments, in which the resistibility to NaCl in whole develomental stages is examined by the emergence rate and the effects of NaCl on the SD action in Drosophila melanogaster. The four SD strains and one mutant strain(cn bw)are used and NaCl media are prepared y adding NaCl at a concentration of 1.0M, 0.7M, 0.5M, 0.3M, 0.1M, and 0.0M to the standard media for the present investigations. The results are given below. 1. The emergence rate (resistibility to NaCl) is not significantly different among strains but strikingly different among concentrations of NaCl. 2. The emergence rate decreases as the concentration of NaCl increases; the four SD strains are considerably resistible to the NaCl from a concentration of 0.0M to 0.3M but are susceptible from 0.5M or higher concentrations of NaCl. 3. No eggs are hatched from the culture media containing a concentration of 1.0M NaCl. This suggests that the SD strains are not resistible to NaCl at a concentrations of 1.0M or higher. 4. The difference in k values is not signficant among strains and also among concentrations of NaCl. Thus the SD action is not affected as far as once emerged from the culture media whether containing NaCl or not.

  • PDF