• The Bulletin of The Korean Astronomical Society
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• v.38 no.2
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• pp.63.2-63.2
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• 2013

Unlike the conventional wisdom, observations made during the past decade have revealed that many globular clusters possess more than one stellar population. Here, we have discovered evidence for multiple red giant branches (RGBs) in the globular cluster NGC 6388 from the narrow-band Calcium and Str$\ddot{o}$mgren b & y (Caby) photometry. In order to confirm the difference in Calcium abundance, we have acquired the low resolution spectroscopy for these RGB stars. In this paper, we will present results of our photometry and hand in the preliminary results of spectroscopic observations.

• BMB Reports
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• v.29 no.3
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• pp.183-191
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• 1996

DNA fragments, homologous to the dTDP-D-glucose 4,6-dehydratase gene, obtained from the genomic DNA of Streptomyces antibioticus $T\ddot{u}99$, a producer of the unusual macrolide antibiotic chlorothricin, were cloned and sequenced. This dehydratase gene was designated as oxil. The coding region of the oxil gene is composed of 987 bp, and analysis of the DNA sequence data reveals sequences for the gene products of 329 amino acids (molecular weight of 36,037). The deduced amino acids are 59% identical to the StrE, dTDP-D-glucose 4,6-dehydratase from the streptomycin pathway. The oxil's function was examined by expressing it in E. coli using the T7 RNA polymerase/promoter system (pRSET) to produce an active fusion protein including a his tag. This enzyme shows specificity of substrate, specific only to dTDP-D-glucose.

• Mass Spectrometry Letters
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• v.8 no.1
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• pp.14-17
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• 2017

The effect of cationization agent concentration on glycan detection via matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was investigated using $Na^+$ ions in the form of NaCl as the cationization agent. NaCl solution concentrations ranging from 1 mM to 1 M were investigated. Glycans from ovalbumin were mixed with the cationization agent solution and the 2,5-dihydroxybenzoic acid (2,5-DHB) matrix solution in a volume ratio of 1:1:1. The resulting mixture was loaded onto the MALDI plate. Two MALDI-TOF MS instruments (Voyager DE-STR MALDI-TOF MS and Tinkerbell RT MALDI-TOF MS) were used for detection of glycans. The best detection, in terms of the number of identified glycans, the peak intensity, and the signal-to-noise (S/N) ratio, was obtained with NaCl concentrations of 0.01-0.1 M for both MALDI-TOF MS instruments.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.9 no.1
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• pp.24-29
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• 2000

This paper is experimental study of machining characteristics about martensitic heat resisting steel STR11. Machining characteristics are different according to internal quality(chemical compositions, microscopic structure and nonmetallic inclusion) mechanical properties(tensile strength value impact value and hardness) and dynamic cutting force. Following are the results : 1. In analyzing internal quality test materials have typical martensite structure and a minute needle-shaped structure. 2. Tensile strength and reduction of area and hardness are larger. But values of elongation and impact values are smaller. Fracture surface of tensile specimen is ductile. 3, Cutting force is decreasing with cutting speed increasing 4. Cutting force is increasing with feed speed increasing.

• International Journal of Precision Engineering and Manufacturing
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• v.2 no.1
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• pp.87-93
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• 2001

A reliable analytic model that determines the exit cross sectional shape a workpiece(material) in round-oval (oroval-round) pass sequence has been developed. The exit cross sectional shape of an outgoing workpiece is predicted by using the linear interpolation of the radius of curvature of an incoming workpiece and that of roll groovw to the roll axis direction. The requirements placed on the choice of the weighting function were to ensure boundary conditions specified. The validity of the analytic model has been examined by not rod rolling experiment with the roll gap and specimen size changed. The exit cross sectional shape and area of the workpiece predicted by the proposed analytic model were good agreement with those obtained experimentally. We found that the analytic model has not only simplicity and accuracy for practical usage but also save a large amount of computational time compared with finite element method.

• Journal of Microbiology and Biotechnology
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• v.20 no.1
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• pp.39-44
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• 2010

Acidithiobacillus caldus is an acidophilic, chemolithotrophic bacterium that plays an important role in bioleaching. Gene transformation into A. caldus is difficult, and only the conjugation method was reported successful, which was a relatively sophisticated method. In this research, electrotransformation of A. caldus species was achieved for the first time using A. caldus Y-3 and plasmid pJRD215. Transformants were confirmed by colony PCR specific to the str gene on pJRD215, and the recovery of the plasmid from the presumptive transformants. Optimizations were made and the transformation efficiency was increased from 0.8 to $3.6{\times}10^4$ transformants/${\mu}g$ plasmid DNA. The developed electrotransformation method was convenient in introducing foreign genes into A. caldus.

• Journal of the Korean Graphic Arts Communication Society
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• v.15 no.1
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• pp.17-30
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• 1997

In this work, we have investigated the printability such as print through and str-off of domestic newspapers. Nine kinds of domestic newspapers Hansol, Deahan, Sinho, Hanla and Sepung, were tested by IGT printability tester, and studied using maximum ink requirement method. We obtained the datum of printability parameters such as maximum ink requirment of paper Ymax, printing density D, print through, set-off and gloss. The results of this test showed that a choice of the fillers on the newspaper had an effect on print through, set-off and density of newspapers in the maximum ink transfer point.

• Journal of the Korean Applied Science and Technology
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• v.29 no.2
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• pp.248-256
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• 2012

Aza-indoles are important pharmacophores that have similar size and biological properties of indole. Here we have synthesized 4- and 7-azaindole tryptamines and showed that they are successfully incorporated in the biosynthesis of monoterepene indole alkaloids (MIAs) to form novel azaindole alkaloids by enzymatic reactions of strictosidine synthase(STR) and strictosidine glucosidase(SDG) monitored by UPLC/MS. By using HPLC equipped with a HPLC photo diode array(PDA) detector, each of the UV spectra of azaindole alkaloids was obtained and characterized. When hydrophilicity of azaindole alkaloids was compared, 4-azaindole alkaloids were more hydrophilic than 7-azaindole alkaloids.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 1998.11a
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• pp.37-40
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• 1998

(Ba.Sr)TiO$_3$(BST) ceramics were fabricated with different Ba/Sr ratios and the str dielectric properties of the BST bulk ceramics were investigated. As the Ba/sr ratios dielectric constant decreased. (Ba$_{0.7}$Sr$_{0.3}$)TiO$_3$ (BST(70/30)) showed a maximum dielec value of $\varepsilon$$_{r}$=8.856, this showed that the decrease of Ba/Sr ratios had made BST thin dielectrics. Targets were fabricated and made into film by PLD process and the thin film by PLD process have good stoichiometry with the targets.rgets.

• Journal of Ginseng Research
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• v.3 no.2
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• pp.113-126
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• 1979

This study was undertaken to assess the effect of the Korean red ginseng extracts on the growth of lactic acid bacteria, such as Lac. acidophilus and Str. thermophilus. The results are summarized as follows: 1. The growth of all the lactic acid bacteria tested in this study was not influenced when the skim milk was added with the red ginseng extracts in concentration of less than 4.0%. But the growth was remarkably inhibited in the presence of 8.0% red ginseng extracts in skim milk. 2. Titratable acidity was more or less increased by addition of red ginseng extracts as compared with control except when the amount of 8.0% red ginseng extracts were added into skim milk. 3. No particular effect was observed in the growth of lactic acid bacteria in case of addition of saponins. But the remarkable inhibitory effect was observed in the skim milk with the amount of 1.2mg/ml the fractions extracted by ethyl ether from red ginseng extracts.