• Journal of the Korean Chemical Society
• /
• v.63 no.6
• /
• pp.427-434
• /
• 2019

The role of hydrophobic residues on protein folding reaction was studied by folding kinetics measurements in conjunction with protein engineering. The HubWA, which was derived from human ubiquitin by mutating the residues at 45 (Phe to Trp) and 26 (Val to Ala), was used as a mutational background. Fourteen hydrophobic residues were mutated to alanine. Among fourteen variants generated, only four variant proteins (V5A, I13A, V17A, and I36A) were suitable for folding study. The folding kinetics of these variants was measured by stopped-flow fluorescence spectroscopy. The folding kinetics of HubWA and V17A was observed to follow a three-state on-pathway mechanism. On the other hand, folding kinetics of V5A, I13A, and I36A was observed to follow a two-state mechanism. Based on these observations, transition of protein folding reaction from collision-diffusion mechanism to nucleation-condensation mechanism was discussed.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
• /
• v.19 no.1
• /
• pp.51-59
• /
• 2007

The waste bunker usually consists of waste entrance zone and waste pit. In this paper, the distributions of air flow, $NH_3$ concentration and $H_2S$ concentration in a waste bunker were investigated to prevent an odor generated in a waste pit from dispersing to the waste entrance zone by numerical method. Four cases were considered such that 1) the waste incinerators is operated, 2) the waste incinerators is stopped, 3) the waste incinerator is operated and the direction of a supply diffuser is $45^{\circ}$ upward, 4) the waste incinerator is stopped and the direction of a supply diffuser is $45^{\circ}$ upward. In case of 1), the fresh air from the waste entrance zone is exhausted smoothly to the main exhaust grill of the waste pit. It means that an odor dispersion to the waste entrance zone will not occur. However in case of 2), the induction of fresh air is so small and the supply air with an odor in waste pit can flow to the waste entrance zone. Therefore, an odor will be dispersed to the waste entrance zone. This paper shows the solution that the supply diffuser with the direction of $45^{\circ}$ upward is chosen. As a result in case of 3) and 4), an odor dispersion to the waste entrance zone does not occurred and on odor is exhausted smoothly to the auxiliary exhaust grill.

• Transactions of the Korean Society of Mechanical Engineers B
• /
• v.24 no.4
• /
• pp.561-569
• /
• 2000

The purpose of this study is to construct a testing equipment with which several characteristics of the domestically developed swashplate type axial piston motor can be tested and to develop a software with which the data from experiment can be stored and can be applied. The results of the study are as follows; 1) The leakage flow and the torque of the motor being stopped is propotional to supply pressure and their relation can be showed by linear equations. 2) The motor movement is not smooth below 50 rpm but it moves smoothly up 170 rpm. 3) When the motor starts or stops, the pressure rise ratio effects decisively to the max. torque.

• Proceedings of the Korean Environmental Sciences Society Conference
• /
• 2003.11a
• /
• pp.109-112
• /
• 2003

A method to determine As(V) ions in aqueous solution by chemiluminescence method has been studied using a stopped flow system. The method is based on the increased chemiluminescence intensity with the addition of As(V) ion to a solution of lucigenin and hydrogen peroxide. The effects of KOH concentration, $H_2O_2$ concentration and flow rate of reagents on the chemiluminescence intensity have been investigated. The calibration curve for As(V) was linear over the range from $1.0{\times}l0^{-6}$M to $1.0{\times}l0^{-4}$M, the coefficient of correlation was 0.997 and the detection limit was $3.3{\times}l0^{-7}$M under the optimal experimental conditions.

• Journal of Sensor Science and Technology
• /
• v.15 no.5
• /
• pp.317-322
• /
• 2006

A method to determine quinine in aqueous solution by chemiluminescence method using a stopped flow system has been studied. The method is based on the increased chemiluminescence intensity with the addition of quinine to a solution of lucigenin and hydrogen peroxide. The effects of KOH concentration, flow rate of reagents, $H_{2}O_{2}$ concentration used for the masking of quinine on the chemiluminescence intensity have been investigated. The calibration curve for quinine was linear over the range from $1.0{\times}10^{-7}$ M to $1.0{\times}10^{-3}$ M, coefficient of correlation was 0.993 and the detection limit was $3.0{\times}10^{-8}$ M under the optimal experimental conditions of 1.0 M, 1.5 M, 3.0 mL/min for the concentration of $H_{2}O_{2}$, KOH and flow rate of reagents, respectively.

• The Journal of The Korea Institute of Intelligent Transport Systems
• /
• v.4 no.1 s.6
• /
• pp.43-56
• /
• 2005

This research has examined a time series analysis(TSA) of an every hour traffic information such as occupancy, a traffic flow, and a speed, a statistical model of a surveyed data on the traffic fundamental diagram and an expand aspect of a traffic jam by many Parts of the traffic flow. Based on the detected data from traffic accidents on the Cheonan-Nonsan high way and events when the road volume decreases dramatically like traffic accidents it can be estimated from the change of occupancy right after accidents. When it comes to a traffic jam like events the changing gap of the occupancy and the mean speed is gentle, in addition to a quickness and an accuracy of a detection by the time series analyse of simple traffic index is weak. When it is a stable flow a relationship between the occupancy and a flow is a linear, which explain a very high reliability. In contrast, a platoon form presented by a wide deviation about an ideal speed of drivers is difficult to express by a statical model in a relationship between the speed and occupancy, In this case the speed drops shifty at 6$\~$8$\%$ occupancy. In case of an unstable flow, it is difficult to adopt a statistical model because the formation-clearance Process of a traffic jam is analyzed in each parts. Taken the formation-clearance process of a traffic jam by 2 parts division into consideration the flow having an accident is transferred to a stopped flow and the occupancy increases dramatically. When the flow recovers from a sloped flow to a free flow the occupancy which has increased dramatically decrease gradually and then traffic flow increases according as the result analyzed traffic flow by the multi regime as time series. When it is on the traffic jam the traffic flow transfers from an impeded free flow to a congested flow and then a jammed flow which is complicated more than on the accidents and the gap of traffic volume in each traffic conditions about a same occupancy is generated huge. This research presents a need of a multi-regime division when analyzing a traffic flow and for the future it needs a fixed quantity division and model about each traffic regimes.

• Journal of Ginseng Research
• /
• v.31 no.1
• /
• pp.23-33
• /
• 2007

Ginsenosides are one of the most well-known traditional herbal medicines frequently used for the treatment of cardiovascular symptoms in korea. The anti-ischemic effects of the mixture of ginsenoside $Rg_3$, and CK on ischemia-induced isolated rat heart were investigated through analyses of changes in hemodynamics ; blood pressure, aortic flow, coronary flow, and cardiac output. The subjects in this study were divided into four groups: normal control, the mixture of ginsenoside $Rg_3$ and CK, an ischemia-induced group without any treatment, and an ischemia-induced group treated with the mixture of ginsenoside $Rg_3$ and CK. There were no significant differences in perfusion pressure, aortic flow, coronary flow and cardiac output between them before ischemia was induced. The supply of oxygen and buffer was stopped for five minutes to induce ischemia in isolated rat hearts, and the mixture of ginsenoside $Rg_3$ and CK was administered during ischemia induction. Treatments of the mixture of ginsenoside $Rg_3$ and CK significantly prevented decreases in perfusion pressure, aortic flow, coronary flow, and cardiac output under ischemic conditions. In addition, hemodynamics (except heart rate) of the group treated with the mixture of ginsenoside $Rg_3$ and CK significantly recovered 60 minutes after reperfusion compared to the control group (mixture+ischemia vs ischemia - average perfusion pressure: 74.4${\pm}$2.97% vs. 85.1${\pm}$3.01%, average aortic flow volume: 49.11${\pm}$2.72% vs. 59.97${\pm}$2.93%, average coronary flow volume: 58.50${\pm}$2.81% vs. 72.72${\pm}$2.99%, and average cardiac output: 52.47${\pm}$2.78% vs. 63.11${\pm}$2.76%, p<0.01, respectively). These results suggest that treatment of the mixture of ginsenoside $Rg_3$ and CK has distinct anti-ischemic effects in ex vivo model of ischemia-induced rat heart.

• Magazine of the Korean Society of Agricultural Engineers
• /
• v.9 no.2
• /
• pp.1296-1300
• /
• 1967

This study was made through the utilization of heavy soil taken from the experimental plot of heavy soil in Konkuk University, Changan-dong, Sungdong-ku, Seoul. The soil used in the experiment has the following physical characteristics: 1. The soil is very compact, impervious, and unfit for any plant growth, 2. For improvement of the soil, tile drainage practice has been employed, 3. According to the general theory of tile drainage, it is unnatural that the effect of drainage is actually observed in such a soil. The followings are the results of the experiment: 1. Water moved to crosswise when the plotted soil profile was not broke. In this case the upper sloped part was dry while the bottom part was moistned. The upper part of the tile was also moistned. 2. The crosswise movement of water was not observed in the artificially broken plot of subsoil. However, the water flow from the tile was observed for long period as a result of the increase of soil void, seepage, aeration, and water holding capacity. However, the water flow from the tile in the plot of unbroken subsoil was observed only in short period and soon the flow was stopped. 3. the distance between the tile laid in the heavy soil should not exceed 10m for the efficient drainage. 4. When the pF is 2.5 in the subsoil the moisture content was between 23.97% and 28.20%. However, when the water saturated in the subsoil the moisture content was between 34.30% and 22.10%. Accordingly without the higher pF than 2.5 the water can not be absorbed and therefore the drainage can not be occured.

• Geotechnical Engineering
• /
• v.11 no.3
• /
• pp.123-138
• /
• 1995

To study the grout flow in jointed rock, various nurser characteristics of grout in a single joint plane and two-dperorbed. The joint plane is described as a channel nets properties of grout are considered. To deal with various prob generator and i oint network generator are used. A loss of head due to friction in laminal flow is adopted to between the grout and joint wall. The grout flow is stopped, setting time. To consider this phenomenon, the idea of maxim From the results of numerical simulation on the single jai etration of grout is confirmed. The basic principles for the ation and the selection of the grout are presented. Correlation ant and grouting pressure is defined by analyzing the effects grout flow. Finally, the grout flow around a tunnel is simulate ins grouting operation for jointed rock mass.

• Archives of Pharmacal Research
• /
• v.29 no.9
• /
• pp.777-785
• /
• 2006

Nelumbinis Semen (NS), or lotus seed, is one of the most well-known traditional herbal medicines and is frequently used to treat cardiovascular symptoms in Korea. The anti-ischemic effects of NS on ischemia-induced isolated rat heart were investigated through analyses of changes in blood pressure, aortic flow, coronary flow, and cardiac output. The subjects in this study were divided into two groups: a control, untreated ischemia-induced group, and an ischemia-induced group treated with NS. There were no significant differences in perfusion pressure, aortic flow, coronary flow and cardiac output between the groups before ischemia was induced. The supply of oxygen and buffer was stopped for ten minutes to induce ischemia in isolated rat hearts, and NS was administered during ischemia induction. NS treatment significantly prevented decreases in perfusion pressure, aortic flow, coronary flow and cardiac output under ischemic conditions (p<0.01). In addition, the mechanism of the anti-ischemic effects of NS was also examined through quantitation of intracellular calcium content in rat neonatal cardiomyocytes. NS significantly prevented intracellular calcium increases induced by isoproterenol (p<0.01). These results suggest that NS has distinct anti-ischemic effects through calcium antagonism.