Browse > Article

Effects of Nelumbinis Semen on Contractile Dysfunction in Ischemic and Reperfused Rat Heart  

Kim, Jong-Hoon (Department of Veterinary Physiology, College of Veterinary Medicine, Chonbuk, National University)
Kang, Moon-Kyu (Purimed R&D Institute, Kyung-Hee University)
Cho, Chong-Woon (Purimed R&D Institute, Kyung-Hee University)
Chung, Hwan-Suck (Purimed R&D Institute, Kyung-Hee University)
Kang, Chang-Woon (Department of Veterinary Physiology, College of Veterinary Medicine, Chonbuk, National University)
Parvez, Shoukat (Purimed R&D Institute, Kyung-Hee University,NIBGE)
Bae, Hyun-Su (Department of Physiology, College of Oriental Medicine, Kyung-Hee University)
Publication Information
Archives of Pharmacal Research / v.29, no.9, 2006 , pp. 777-785 More about this Journal
Abstract
Nelumbinis Semen (NS), or lotus seed, is one of the most well-known traditional herbal medicines and is frequently used to treat cardiovascular symptoms in Korea. The anti-ischemic effects of NS on ischemia-induced isolated rat heart were investigated through analyses of changes in blood pressure, aortic flow, coronary flow, and cardiac output. The subjects in this study were divided into two groups: a control, untreated ischemia-induced group, and an ischemia-induced group treated with NS. There were no significant differences in perfusion pressure, aortic flow, coronary flow and cardiac output between the groups before ischemia was induced. The supply of oxygen and buffer was stopped for ten minutes to induce ischemia in isolated rat hearts, and NS was administered during ischemia induction. NS treatment significantly prevented decreases in perfusion pressure, aortic flow, coronary flow and cardiac output under ischemic conditions (p<0.01). In addition, the mechanism of the anti-ischemic effects of NS was also examined through quantitation of intracellular calcium content in rat neonatal cardiomyocytes. NS significantly prevented intracellular calcium increases induced by isoproterenol (p<0.01). These results suggest that NS has distinct anti-ischemic effects through calcium antagonism.
Keywords
Nelumbinis Semen; Traditional herbal medicine; Anti-ischemic effect; Cardiomyocyte; Isoproterenol; Calcium antagonism;
Citations & Related Records

Times Cited By Web Of Science : 4  (Related Records In Web of Science)
Times Cited By SCOPUS : 4
연도 인용수 순위
1 Asano, G., Takashi, E., Ishiwata, T., Onda, M., Yokoyama, M., Naito, Z., Ashraf, M., and Sugisaki, Y., Pathogenesis and protection of ischemia and reperfusion injury in myocardium. J. Nippon Med. Sch., 70, 384-392 (2003)   DOI
2 Nieto-Sampedro, M., Shelton, D., and Cotman, C. W., Specific binding of kainic acid to purified subcellular fractions from rat brain. Neurochem. Res., 5, 591-604 (1980)   DOI
3 Wang, J. L., Nong, Y., Xia, G. J., Yao, W. X., and Jiang, M. X., Effects of linesinine on slow action potentials in myocardium and slow inward current in canine cardiac Purkinje fibers. Yao Xue Xue Bao, 28, 812-816 (1993)
4 Zelenski, S. G., Alkaloids of Nelumbo lutea (Wild.) pers. (Nymphaeaceae). J. Pharm. Sci., 66, 1627-1628 (1977)   DOI
5 Lozoya, X., Meckes, M., Abou-Zaid, M., Tortoriello, J., Nozzolillo, C., and Arnason, J. T., Quercetin glycosides in Psidium guajava L. leaves and determination of a spasmolytic principle. Arch. Med. Res., 25, 11-15 (1994)
6 Qian, J. Q., Cardiovascular pharmacological effects of bisbenzylisoquinoline alkaloid derivatives. Acta. Pharmacol. Sin., 23, 1086-1092 (2002)
7 Yu, X. C., Wu, S., Wang, G. Y., Shan, J., Wong, T. M., Chen, C. F., and Pang, K. T., Cardiac effects of the extract and active components of radix stephaniae tetrandrae. II. Myocardial infarct, arrhythmias, coronary arterial flow and heart rate in the isolated perfused rat heart. Life Sci., 68, 2863-2872 (2001)   DOI   ScienceOn
8 Li, G. R., Li, X. G., and Lu, F. H., Effects of neferine on transmembrane potential in rabbit sinoatrial nodes and clusters of cultured myocardial cells from neonatal rats. Zhongguo Yao Li Xue Bao, 10, 328-331 (1989a)
9 Li, G. R., Qian, J. Q., and Lu, F. H. [Effects of neferine on heart electromechanical activity in anaesthetized cats]. Zhongguo Yao Li Xue Bao, 11, 158-161 (1990)
10 Li, G. R., Li, X. G., and Lu, F. H., Effects of neferine on transmembrane potentials of guinea pig myocardium. Zhongguo Yao Li Xue Bao, 10, 406-410 (1989b)
11 Meng, D., Chen, X. J., Bian, Y. Y., Li, P., Yang, D., and Zhang, J. N., Effect of astragalosides on intracellular calcium overload in cultured cardiac myocytes of neonatal rats. Am. J. Chin. Med., 33, 11-20 (2005)   DOI   ScienceOn
12 Kang, M., Pyun, K. H., Jang, C. G., Kim, H., Bae, H., and Shim, I., Nelumbinis Semen reverses a decrease in hippocampal 5- HT release induced by chronic mild stress in rats. J. Pharm. Pharmacol., 57, 651-656 (2005)   DOI   ScienceOn
13 Sohn, D. H., Kim, Y. C., Oh, S. H., Park, E. J., Li, X., and Lee, B. H., Hepatoprotective and free radical scavenging effects of Nelumbo nucifera. Phytomedicine, 10, 165-169 (2003)   DOI   ScienceOn
14 Jang, C. G., Kang, M., Cho, J. H., Lee, S. B., Kim, H., Park, S., Lee, J., Park, S. K., Hong, M., Shin, M. K., Shim, I. S., and Bae, H., Nelumbinis Semen reverses a decrease in 5-HT1A receptor binding induced by chronic mild stress, a depressionlike symptom. Arch. Pharm. Res., 27, 1065-1072 (2004)   DOI   ScienceOn
15 Kang, M. S., D. Oh, J. W. Cho, C. Lee, H. J. Yoon, D. W. Lee, S. M. Yun, J. H. Choi, H. Park, S. Shin, M. Hong, M., and Bae, H., The anti-depressant effect of Nelumbinis Semen on rats under chronic mild stress inducing depression-like symptoms. Am. J. Chin. Med., 33, 205-213 (2005)   DOI   ScienceOn
16 Shirai, K., Obesity as the core of the metabolic syndrome and the management of coronary heart disease. Curr. Med. Res. Opin., 20, 295-304 (2004)   DOI   ScienceOn
17 Wang, J., Hu, X., Yin, W., and Cai, H., Alkaloids of plumula Nelumbinis. Zhongguo Zhong Yao Za Zhi, 16, 673-675, 703 (1991)
18 la Cour, B., Molgaard, P., and Yi, Z. Traditional Chinese medicine in treatment of hyperlipidaemia. J. Ethnopharmacol., 46, 125- 129 (1995)   DOI   ScienceOn
19 Mehta, R. H., Das, S., Tsai, T. T., Nolan, E., Kearly, G., and Eagle, K. A., Quality improvement initiative and its impact on the management of patients with acute myocardial infarction. Arch. Intern. Med., 160, 3057-3062 (2000)   DOI   ScienceOn
20 Zou, Y., Lu, Y., and Wei, D., Antioxidant activity of a flavonoidrich extract of Hypericum perforatum L. in vitro. J. Agric. Food Chem., 52, 5032-5039 (2004)   DOI   ScienceOn
21 Galagudza, M., Kurapeev, D., Minasian, S., Valen, G., and Vaage, J., Ischemic postconditioning: brief ischemia during reperfusion converts persistent ventricular fibrillation into regular rhythm. Eur. J. Cardiothorac. Surg., 25, 1006-1010 (2004)   DOI   ScienceOn
22 Li, X. S., Uriuda, Y., Wang, Q. D., Norlander, R., Sjoquist, P. O., and Pernow, J., Role of L-arginine in preventing myocardial and endothelial injury following ischaemia/reperfusion in the rat isolated heart. Acta. Physiol. Scand., 156, 37-44 (1996)   DOI   ScienceOn
23 Sun, J., Tan, B. K., Huang, S. H., Whiteman, M., and Zhu, Y. Z., Effects of natural products on ischemic heart diseases and cardiovascular system. Acta. Pharmacol. Sin., 23, 1142-1151 (2002)
24 Wu, S., Sun, C., Cao, X., Zhou, H., Hong, Z., and Pan, Y. Preparative counter-current chromatography isolation of liensinine and its analogues from embryo of the seed of Nelumbo nucifera GAERTN. using upright coil planet centrifuge with four multilayer coils connected in series. J. Chromatogr. A., 1041, 153-162 (2004)   DOI   ScienceOn
25 Yu, J., and Hu, W. S. Effects of neferine on platelet aggregation in rabbits. Yao Xue Xue Bao, 32, 1-4, (1997)