• 한국지능시스템학회:학술대회논문집
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• 한국퍼지및지능시스템학회 2001년도 추계학술대회 학술발표 논문집
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• pp.56-59
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• 2001

In this paper, unmaned fully automation systems are applied for the CSTR(Continuously Stirred Tank Reactor) and, SBR (Sequencing Batch Reactor) wastewater treatment pilot plant. This plant is constructed in the country side which is little far from a main city. So networks and wireless modules are employed for the data transmission. The SBR plant has a local control and monitoring system which is contained communication parts which consist of one ADSL (Asymmetric Digital Subscriber Line) network and one CDMA (Code Division Multiple Access) module. Remote control and monitoring systems are constructed at a laboratory in a metropolis.

• Journal of Microbiology and Biotechnology
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• 제14권6호
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• pp.1129-1133
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• 2004

The effects of Pluronic F-68, a non-ionic surfactant, on the growth and physical characteristics of Digitalis lanata suspension cultures were investigated in aqueous two-phase systems (ATPSs) composed of $4.5\%$ polyethylene glycol (PEG) 20,000 and $2.8\%$ crude dextran. In the range of 0.1-10.0 g $1^{-1}$, Pluronic F-68 enhanced the maximum cell density in a medium with ATPSs, even though Pluronic F-68 did not affect cell growth in a normal growth medium. In terms of physical properties of ATPSs with cell suspension cultures, 0.2 g $1^{-1}$ of Pluronic F-68 reduced viscosity by up to $40\%$, while 0.1 g $1^{-1}$ of Pluronic F-68 significantly enhanced the oxygen transfer rate. In addition, we successfully performed aqueous two-phase cultivation in a 5-1 stirred tank bioreactor with 0.5 g $1^{-1}$ of Pluronic F-68, and discovered that cell growth in ATPSs was similar to that in normal growth medium.

• Journal of Microbiology and Biotechnology
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• 제21권4호
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• pp.430-437
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• 2011

The effects of the agitation and aeration rates on the production of serratiopeptidase (SRP) in a 5-L fermentor (working volume 2-l) were systematically investigated using Serratia marcescens NRRL B-23112. The dissolved oxygen concentration, pH, biomass, SRP yield, and maltose utilization were all continuously measured during the course of the fermentation runs. The efficiencies of the aeration and agitation were evaluated based on the volumetric mass transfer coefficient ($K_La$). The maximum SRP production of 11,580 EU/ml with a specific SRP productivity of 78.8 EU/g/h was obtained with an agitation of 400 rpm and aeration of 0.075 vvm, which was 58% higher than the shake-flask level. The $K_La$ for the fermentation system supporting the maximum production (400 rpm, 0.075 vvm) was 11.3 $h^{-1}$. Under these fermentor optimized conditions, kinetic modeling was performed to understand the detailed course of the fermentation process. The resulting logistic and Luedeking-Piret models provided an effective description of the SRP fermentation, where the correlation coefficients for cell growth, SRP formation, and substrate consumption were 0.99, 0.94, and 0.84, respectively, revealing a good agreement between the model-predicted and experimental results. The kinetic analysis of the batch fermentation process for the production of SRP demonstrated the SRP production to be mixed growth associated.

• Journal of Microbiology and Biotechnology
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• 제19권12호
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• pp.1603-1611
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• 2009

High-cell-density cultivation of yeast was investigated using the agricultural waste products corn steep liquor (CSL) and molasses. The Saccharomyces cerevisiae KV-25 cell mass was significantly dependent on the ratio between C and N sources. The concentrations of molasses and CSL in the culture medium were statistically optimized at 10.25% (v/v) and 16.87% (v/v), respectively, by response surface methodology (RSM). Batch culture in a 5-l stirred tank reactor using the optimized medium resulted in a cell mass production of 36.5 g/l. In the fed-batch culture, the feed phase was preceded by a batch phase using the optimized medium, and a very high dried-cell-mass yield of 187.63 g/l was successfully attained by feeding a mixture of 20% (v/v) molasses and 80% (v/v) CSL at a rate of 22 ml/h. In this system, the production of cell mass depended mainly on the agitation speed, the composition of the feed medium, and the glucose level in the medium, but only slightly on the aeration rate.

• Environmental Engineering Research
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• 제19권4호
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• pp.387-393
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• 2014

The purpose of this study is to evaluate integrated anaerobic hydrogen fermentation and membrane bioreactor (MBR) for on-site domestic wastewater treatment and resource recovery. A synthetic wastewater (COD 17,000 mg/L) was used as artificial brown water which will be discharged from urine diversion toilet and fed into a continuous stirred tank reactor (CSTR) type anaerobic reactor with inclined plate. The effluent of anaerobic reactor mixed with real household grey water (COD 700 mg/L) was further treated by MBR for reuse. An optimum condition maintained in anaerobic reactor was HRT of 8 hrs, pH 5.5, SRT of 5 days and temperature of $37^{\circ}C$. COD removal of 98% was achieved from the overall system. Total gas production rate and hydrogen content was 4.6 L/day and 52.4% respectively. COD mass balance described the COD distribution in the system via reactor byproducts and effluent COD concentration. The results of this study asserts that, anaerobic hydrogen fermentation combined with MBR is a potent system in stabilizing waste strength and clean hydrogen recovery which could be implemented for onsite domestic wastewater treatment and reuse.

• 제어로봇시스템학회논문지
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• 제19권9호
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• pp.839-845
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• 2013

A CSTR (Continuous Stirred Tank Reactor) is a highly nonlinear process with varying parameters during operation. Therefore, tuning of the controller and determining the transition policy of controller parameters are required to guarantee the best performance of the CSTR for overall operating regions. In this paper, a methodology employing the 2DOF (Two-Degree-of-Freedom) PID controller, the anti-windup technique and a fuzzy gain scheduler is presented for the temperature control of the CSTR. First, both a local model and an EA (Evolutionary Algorithm) are used to tune the optimal controller parameters at each operating region by minimizing the IAE (Integral of Absolute Error). Then, a set of controller parameters are expressed as functions of the gain scheduling variable. Those functions are implemented using a set of "if-then" fuzzy rules, which is of Sugeno's form. Simulation works for reference tracking, disturbance rejecting and noise rejecting performances show the feasibility of using the proposed method.

• 제어로봇시스템학회논문지
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• 제21권5호
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• pp.482-489
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• 2015

CSTR (Continuous Stirred Tank Reactor) which plays a key role in the chemical plants exhibits highly nonlinear behavior as well as time-varying behavior during operation. The control of CSTRs in the whole operating range has been a challenging problem to control engineers. So, a variety of feedback control forms and their tuning methods have been implemented to guarantee the satisfactory performance. This paper presents a scheme of designing a nonlinear PID controller incorporating with a GA (Genetic Algorithm) for the temperature control of a CSTR. The gains of the NPID controller are composed of easily implementable nonlinear functions based on the error and/or the error rate and its parameters are tuned using a GA by minimizing the ITAE (Integral of Absolute Error). Simulation works for reference tracking and disturbance rejecting performances and robustness to parameter changes show the feasibility of the proposed method.

• Korean Chemical Engineering Research
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• 제47권3호
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• pp.380-385
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• 2009

평판형 교반기를 사용하여 2-amino-2-methyl-1-propanol(AMP)가 용해된 methanol, ethanol, n-propanol, n-butanol, ethylene glycol, propylene glycol, 및 propylene carbonate와 같은 극성 용매에서 이산화탄소($CO_2$)의 흡수속도를 측정하였다. $CO_2$의 흡수속도와 carbamate 생성 반응 메커니즘을 사용하여 기-액 불균일반응계의 빠른 반응영역에서 $CO_2$-AMP의 반응속도론을 해석하였으며 용매의 용해도 매개변수와 반응속도상수와의 상관관계를 제시하였다.

• 한국응용과학기술학회지
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• 제16권1호
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• pp.75-81
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• 1999

HF purification performance of an ion exchange membrane(IEM) was evaluated with 0.5% HF spiked with 10ppb of Fe, Ni and Cu nitrates. The result show that after less than five turnovers through an IEM, the metallic impurity concentration drops below 1ppb. The decrease rate can be fitted to a model assuming the experimental tanks to be continuously stirred tank reaction and that the metallic impurity concentration after the IEM is a function of the single-pass purification efficiency of the membrane, the concentration before purification and the metals desorbed form the IEM. The Concentration after purification was investigated up to a cumulative Fe loading of 300ppb in the 23 liter recirculated loop. It increases linearly vs. cumulative loading and can be explained by the Langmuir theory resulting in a purification efficiency at the equilibrium of close to 99.5% in this loading regime.

• 한국지능시스템학회논문지
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• 제11권9호
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• pp.844-848
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• 2001

This paper introduces unmaned fully automation systems, which are applied for the CSTR(Continuously Stirred Tank Reactor) and SBR (Sequencing Batch Reactor) wastewater treatment system. The pilot plant is constructed in the country side which is little far from a main city. So networks and wireless modules are employed for the data transmission. The SBR plant has a local control and the remote monitoring system which is contained communication parts which consist of ADSL (Asymmetric Digital Subscriber Line) network and CDMA (Code Division Multiple Access) Wireless module. Remote control and monitoring systems are constructed at laboratory in a metropolis.