• Title/Summary/Keyword: Stem Form

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In-vitro and In-vivo Biocompatibility Evaluation of Silica Based Bio-active Glass Prepared by Hydrothermal Method

  • Sarkar, Swapan Kumar;Nguyen, Phuong Thi;Lee, Byong-Taek
    • Proceedings of the Materials Research Society of Korea Conference
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    • 2011.05a
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    • pp.53.1-53.1
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    • 2011
  • Bioactive glass powders were synthesized by hydrothermal chemical route by the use of ultrasonic energy irradiation. We used sodalime, calcium nitrate tetra hydrate and di ammonium hydrogen phosphate as the precursor material to synthesize $SiO_2$ rich bio-active glass materials. The $SiO_2$ content was varied in the precursor mixture to 60, 52 and 45 mole%. Dense compacts were obtained by microwave sintering at $1,100^{\circ}C$. Mechanical properties were characterized for the fabricated dense bioactive glasses and were found to be comparable with conventional CaO-$SiO_2$-$Na_2O$-$P_2O_5$ bioactive glass. Detailed biocompatibility evaluation of the glass composition was investigated by in-vitro culture of MG-63 cell and mesenchyme stem cell. Cell adhesion behavior was investigated for both of the cell by one cell morphology for 30, 60 and 90 minutes. Cell proliferation behavior was investigated by culturing both of the cells for 1, 3 and 7 days and was found to be excellent. Both SEM and confocal laser scanning microscopy were used for the investigation. Western blot analysis was performed to evaluate the bimolecular level interaction and extent and rate of specific protein expression. The ability to form biological apatite in physiological condition was observed with simulated body fluid (SBF). In-vivo bone formation behavior was investigated after implanting the materials inside rabbit femur for 1 and 3 month. The bone formation behavior was excellent in all the bioglass compositions, specially the composition with 60% $SiO_2$ content showed most promising trend.

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Anatomical Characteristics of Paulownia tomentosa Root Wood

  • Qi, Yue;Jang, Jaehyuk;Hidayat, Wahyu;Lee, Aehee;Park, Sehwi;Lee, Seunghwan;Kim, Namhun
    • Journal of the Korean Wood Science and Technology
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    • v.44 no.2
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    • pp.157-165
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    • 2016
  • This study investigated several anatomical characteristics of Paulownia tomentosa roots. The root wood was separated into three parts from stem base (top, middle, and base) at different positions below ground. Qualitative anatomical data suggested that the growth rings in earlywood and latewood were structurally different. Furthermore, the root wood vessels were found having 2 to 3 radial multiples and they were appeared in the form of clusters. In addition, some sheath cells and septate axial parenchyma were observed. Regarding the quantitative anatomical characteristics, vessel and ray numbers per $mm^2$, as well as ray width and height differed significantly among the top, middle, and base rood wood parts. However, there were no significant differences in vessel diameters, cell wall thickness, and width and length of wood fibers among those parts. The crystallinity of the root top part was slightly higher than that of the middle and base parts. Furthermore, the vessel numbers, ray numbers, and ray width and height in the near pith (NP) area were higher compared to those in the near bark (NB) area. However, the fiber width and fiber length at NP were lower than those at NB. Overall, this study demonstrated some significant differences in the anatomical characteristics of the top, middle, and base parts of root wood from Paulownia tomentosa.

Production of IL-1β and Inflammasome with Up-Regulated Expressions of NOD-Like Receptor Related Genes in Toxoplasma gondii-Infected THP-1 Macrophages

  • Chu, Jia-Qi;Shi, Ge;Fan, Yi-Ming;Choi, In-Wook;Cha, Guang-Ho;Zhou, Yu;Lee, Young-Ha;Quan, Juan-Hua
    • Parasites, Hosts and Diseases
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    • v.54 no.6
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    • pp.711-717
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    • 2016
  • Toxoplasma gondii is an obligate intracellular parasite that stimulates production of high levels of proinflammatory cytokines, which are important for innate immunity. NLRs, i.e., nucleotide-binding oligomerization domain (NOD)-like receptors, play a crucial role as innate immune sensors and form multiprotein complexes called inflammasomes, which mediate caspase-1-dependent processing of $pro-IL-1{\beta}$. To elucidate the role of inflammasome components in T. gondiiinfected THP-1 macrophages, we examined inflammasome-related gene expression and mechanisms of inflammasome-regulated cytokine $IL-1{\beta}$ secretion. The results revealed a significant upregulation of $IL-1{\beta}$ after T. gondii infection. T. gondii infection also upregulated the expression of inflammasome sensors, including NLRP1, NLRP3, NLRC4, NLRP6, NLRP8, NLRP13, AIM2, and NAIP, in a time-dependent manner. The infection also upregulated inflammasome adaptor protein ASC and caspase-1 mRNA levels. From this study, we newly found that T. gondii infection regulates NLRC4, NLRP6, NLRP8, NLRP13, AIM2, and neuronal apoptosis inhibitor protein (NAIP) gene expressions in THP-1 macrophages and that the role of the inflammasome-related genes may be critical for mediating the innate immune responses to T. gondii infection.

Introduction of Maize Transposable Elements, Ac and Ds into the Genome of a Diploid Potato Species (옥수수 전위유전자 Ac 및 Ds의 2배체종 감자 Genome 내로의 도입)

  • 김화영;임용표
    • Korean Journal of Plant Tissue Culture
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    • v.27 no.1
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    • pp.39-45
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    • 2000
  • Two maize transposable elements, immobilized Ac (iAc) and Ds, have been introduced into the genome of a diploid potato clone (Solanum tuberosum Group Phureja clone 1.22). The iAc is a modified Ac that is supposed to be unable to transpose but is expected to trans-activate the transposition of a Ds that is unable to transpose by itself. When the leaf and stem explants of in vitro shoots of the clone 1.22 were inoculated with Agrobacterium tumefaciens strains harboring binary vectors containing the iAc and the Ds, calli were formed from the explants on media containing 50 mg/L of kanamycin, and shoots were regenerated from the calli. The regenerated shoots formed roots when cultured on media containing 100 mg/L of kanamycin, whereas untransformed shoots did not form roots on the same media. The PCR amplification of the DNA's from the transgenic plants confirmed that the iAc and the Ds elements were introduced into the potato genome of 1.22.

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Characteristics of Kenaf Fibers Treated by Alkali (알칼리 처리에 따른 케나프 섬유의 특성 변화 연구)

  • Yoo, Hye-Ja;Lee, Hye-Ja
    • Journal of the Korean Society of Clothing and Textiles
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    • v.35 no.8
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    • pp.982-990
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    • 2011
  • Kenaf fiber can be obtained by decortications of the kenaf plant stem. The properties of the kenaf fiber treated by alkali (NaOH) were investigated by spectrocolorimeter, SEM, X-ray diffractometer, FT-IR and TGA. The kenaf fibers treated by alkali became darker and their Munsell color values changed from Y (yellow) to YR (yellowred) according to an increased NaOH concentration. SEM observation of the kenaf fibers showed that their crimps were developed and their surfaces were cleaned by the removal of protruding ends and impurities after alkali treatments. In the x-ray diffraction analysis, the structures of the fibers were found in the form of cellulose I when treated with a 0-16% alkali concentration and cellulose II when treated with over 20%. It was also confirmed that the crystallinity was lowered according to an increased NaOH concentration. The change of fiber compositions was investigated in FT-IR analysis. Strong band of $1,738cm^{-1}$ and asymmetrical stretching strong bands of $1,630-1,600cm^{-1}$ in spectrum (which represent pectin) were not found in the samples because the pectin was removed by the alkali treatment. Weak bands of $1,728-1,730cm^{-1}$ and peaks of $1,245-1,259cm^{-1}$ (which represent hemicellulose) and peaks of $1,592cm^{-1}$, $1,504cm^{-1}$, $1,462cm^{-1}$ and $1,429cm^{-1}$ (which are related to lignin) were not found or reduced in the samples treated with a concentration over 20%. TGA indicated that the kenaf fiber had the better hydrophilic properties by alkali treatment. The higher Tmax in TGA and the higher thermal stability when treated by alkali with the higher concentration. The fibers treated with an alkali concentration over 30% did not show any changes in Tmax.

Antioxidant activity of phalaenopsis Sogo Yukidian 'V3' plant parts

  • Truong, Ngoc Minh;Phung, Thi Tuyen;Do, Tan Khang;Nguyen, Van Quan;Pham, Thi Thu Ha;Nguyen, Thanh Quan;Andriana, Yusuf;Truong, Mai Van;Tran, Dang Xuan
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.34-34
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    • 2017
  • Various extracts of roots, stems, and leaves of Phalaenopsis Sogo Yukidian 'V3' were evaluated for total phenolics, total flavonoids, and antioxidant capacity. The conjugate form of stem samples contained the highest total phenolics ($5.092{\pm}0.739mg$ gallic acid equivalent per g dry weight) and the highest total flavonoids ($2.218{\pm}0.021mg$ rutin equivalent per g dry weight) was found in the hexane extract of leaves. The ethyl acetate extract of roots showed the maximum antioxidant activity as compared to other extracts. Of which, the $IC_{50}$ value of this sample were 0.070 mg/mL and 0.450 mg/mL in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and reducing power, respectively, while the lipid peroxidation inhibition (LPI) value was as 94.2% by ${\beta}$-carotene bleaching method. Five phenolic compounds including caffeic acid, syringic acid, vanillin, ellagic acid, and cinnamic acid were quantified by high-performance liquid chromatography (HPLC). It is suggested that roots of the hybrid Phalaenopsis Sogo Yukidian 'V3' can be explodited as an effective source of antioxidants.

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Development of a Stream Water Quality Model (QUAL-NIER) for the Management of Total Maximum Daily Loads (수질오염총량관리를 위한 하천수질모델(QUAL-NIER) 개발)

  • Park, Jun Dae;Shin, Dong Seok;Kim, Moon Sook;Kong, Dong Soo;Rhew, Doug Hee;Jung, Dong-Il;Na, Eun Hye
    • Journal of Korean Society on Water Environment
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    • v.24 no.6
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    • pp.784-792
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    • 2008
  • Greater focus must be placed on ensuring that the water quality model (WQM) reflects the objective of its application and the characteristics of the water environment properly before it is selected. In the development or application of WQM, various factors influencing the model predictions should be reviewed so that it can perform more properly and reasonably based on scientific theory. This study reviewed the characteristic of existing WQM and the domestic river environment to find the requirements of the model application for TMDLs management in Korea. In this study, a water quality model, QUAL-NIER, was developed based on the USEPA's QUAL2E. The core structure and reaction scheme of the model was established followed by the formulation of equations according to the scheme with some supplements on the reaction mechanisms which are necessary for domestic rivers. Algorithms on the equations were set up and programmed to form a computer-based model. The developed model, QUAL-NIER was applied to the main stem of the Nakdong river. The model was calibrated and verified to data measured in 2004. The model results displayed good agrement with the field measurements for both calibration and verification. From this study, it was concluded that the developed QUAL-NIER model was very powerful with regard to the water quality simulation in domestic rivers.

Effect of Inclusion of Lacquer (Rhus verniciflua Stokes) Meal on Carcass Traits and Meat Quality in Growing-finishing Pigs

  • Song, Chang-Hyun;Kim, Jin-Soo;Shinde, Prashant;Kim, Young-Woo;Kim, Kwang-Hyun;Kwon, Ill-Kyung;Kang, Sun-Moon;Lee, Sung-Ki;Chae, Byung-Jo
    • Food Science of Animal Resources
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    • v.30 no.4
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    • pp.597-602
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    • 2010
  • In this study, pigs [n=117; (Landrace ${\times}$ Yorkshire) ${\times}$ Duroc; $64{\pm}0.5$ Kg initial body weight] were used to investigate the effect of feeding different levels of lacquer (Rhus verniciflua Stokes) meal on performance, carcass traits and quality of meat kept under refrigeration at $3{\pm}1^{\circ}C$. The pigs were randomly allotted to 3 treatments on the basis of body weight and sex and each treatment was replicated 3 times (13 pigs in each replicate). Lacquer meal in sawdust form obtained from the stem bark and heartwood of sun-dried lacquer trees was added to the grower and finisher diets at 0, 20 and 40 g/kg diet. The experimental diets were fed for 8 wk. Inclusion of lacquer meal had no influence (p>0.05) on growth performance of pigs. Improvement in carcass traits and decreased back fat thickness were noticed in pigs fed diets added with 20 and 40 g/kg lacquer meal. Longissimus muscle obtained from pigs fed lacquer meal had higher moisture and lower fat content, thiobarbituric acid reactive substances and water holding capacity. Meat from lacquer fed pigs was also darker and redder. The data indicates that lacquer meal can be incorporated up to 40 g/kg in the diet of fattening pigs without affecting growth performance. Also, lacquer meal increases carcass lean content and improves the oxidative stability of the meat.

Establishment of Western-style Court Dress and its Formal Characteristics in the Meiji Period of Japan (일본 메이지기 [明治期] 문관대례복의 성립과 형태적 특징)

  • Lee, Kyung-Mee
    • Journal of the Korean Society of Costume
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    • v.60 no.5
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    • pp.71-87
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    • 2010
  • The purpose of the study is analyzing Japanese modern costume, through examining legislation process and the relics of Chickimkwan's and Juimkwan's court costume. The results of the study are as follows. First, the proposer of civil court costume, established in 1872, was Iwakura Mission dispatched to America and Europe. The Mission realized the importance of preparing western-style costume in civilization from experience wearing traditional clothing at ceremony of presenting credentials in America. Afterwards, the Mission proposed that the government accept western-style as civil court costume and became first wearers in Japanese in England. Second, the difference, between ordinance and actual clothes worn by Iwakura Mission, occurred in process of legislation in 1872. That might be considered as trial and error in introducing different culture. The coexistence of England and French styles was unified into French style by the revision of civil court costume in 1886. Third, the pattern of paulownia embroidered on civil court costume was utilized as symbol of Japan. While the costume of Chickimkwan was embroidered by the pattern of 7 and 5 leaves paulownia, that of Juimkwan was 5 and 3 leaves expressing their grades. Fourth, relics research showed how formed manufacturer information and enacted design were in embroidery. The relics seemed to be manufactured in Japan, because emblem of Mitsukosi tailor was embroidered on inner part of the back of collar of Chickimkwan in Nara Women's University, Japan and that of Yamasaki on left inner pocket of Juimkwan in the Independence Hall, Korea. The embroider techniques comprised forming by filler particles according to the design, filling up coiled gold threads and expressing stem with gold threads and spangles. As preemptive study, establishment process of Japanese civil court costume in this study will help understand form characteristics appeared in civil court costume act of Korean Empire.

A Fundamental Study on Offshore Structures of high pressure control valve (해양구조물용 고압 컨트롤 밸브에 대한 기초 연구)

  • Lee, Chi-Woo;Jang, Sung-Cheol
    • Journal of the Korean Society of Manufacturing Technology Engineers
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    • v.19 no.6
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    • pp.883-888
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    • 2010
  • This study have goal with conceptual design for Offshore Structures of high pressure control valve for localization. Ball valve for development accomplished with flow analysis based on provision of ANSI B16.34, ANSI B16.10, ANSI B16.25 In order to localize the Offshore Structures high pressure control valve. Numerical simulation using CFD (Computational Fluid Dynamic) in order to predict a mass flow rate and a flow coefficient form flow dynamic point of view. The working fluid assumed the glycerin (C3H8O3). The valve inlet and outlet setup a pressure boundary condition. The outlet pressure was fixed by atmospheric pressure and calculated until increasing 1bar to 10bar. CFD analysis used STAR-CCM+ which is commercial code and Governing equations were calculated by moving mesh which is rotated 90 degrees when ball valve operated opening and closing in 1 degree interval. The result shows change of mass flow rate according to opening and closing angle of valve, Flow decrease observed open valve that equal percentage flow paten which is general inclination of ball valve. Relation with flow and flow coefficient can not be proportional according to inlet pressure when compare with mass flow rate. Because flow coefficient have influence in flow and pressure difference. Namely, flow can be change even if it has same Cv value. The structural analysis used ANSYS which is a commercial code. Stress analysis result of internal pressure in valve showed lower than yield strength. This is expect to need more detail design and verification for stem and seat structure.