• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.4
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• pp.358-362
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• 2004

In order to develop molecular markers related to stay green phenotype, AFLP analysis was conducted using near-isogenic lines for either stay green or non stay green trait. Both lines have characteristics of multi-ear and tillers (MET). Two out of 64 primer combinations of selective amplification identified three reproducible polymorphic fragments in MET corn with stay green. Both of E+AGC/M+CAC and E+AAG/M+CAA primer combinations produced two and one specific polymorphic fragments linked to stay green trait, respectively. For the conversion of AFLPs to sequence tag sites (STSs), primers were designed form both end sequences of each two polymorphic fragments. One fragment, which was amplified with E+AAG/M+CAA primer combinations, possessed 298 bp long and showed a $91\%$ homology with maize retrotransposon Cinful-l. One out of two polymorphic fragments produced with E+AGC/M+CAC primer combination had 236 bp long and matched a $96\%$ homology with an intron region of 22kDa alpha zein gene cluster in Zea mays. One out of two PCR fragments amplified with MET2 primer set in the stay green MET was not produced in the non-stay green MET. The developed AFLP and STS marker could be used as an efficient tool for selection of the stay green trait in the MET inbred.

• Molecules and Cells
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• v.24 no.1
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• pp.83-94
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• 2007

During monocarpic senescence in higher plants, functional stay-green delays leaf yellowing, maintaining photosynthetic competence, whereas nonfunctional stay-green retains leaf greenness without sustaining photosynthetic activity. Thus, functional stay-green is considered a beneficial trait that can increase grain yield in cereal crops. A stay-green japonica rice 'SNU-SG1' had a good seed-setting rate and grain yield, indicating the presence of a functional stay-green genotype. SNU-SG1 was crossed with two regular cultivars to determine the inheritance mode and identify major QTLs conferring stay-green in SNU-SG1. For QTL analysis, linkage maps with 100 and 116 DNA marker loci were constructed using selective genotyping with $F_2$ and RIL (recombinant inbred line) populations, respectively. Molecular marker-based QTL analyses with both populations revealed that the functional stay-green phenotype of SNU-SG1 is regulated by several major QTLs accounting for a large portion of the genetic variation. Three main-effect QTLs located on chromosomes 7 and 9 were detected in both populations and a number of epistatic-effect QTLs were also found. The amount of variation explained by several digenic interactions was larger than that explained by main-effect QTLs. Two main-effect QTLs on chromosome 9 can be considered the target loci that most influence the functional stay-green in SNU-SG1. The functional stay-green QTLs may help develop low-input high-yielding rice cultivars by QTL-marker-assisted breeding with SNU-SG1.

• Journal of Crop Science and Biotechnology
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• v.11 no.1
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• pp.75-82
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• 2008

Functional stay-green is a beneficial trait that may increase grain yield through the sustained photosynthetic competence during monocarpic senescence in cereal crops. The temporal changes of photosynthesis and related characteristics throughout the grain filling period of a stay-green japonica rice "SNU-SG1" was compared in growth chamber conditions with three high-yielding cultivars(HYVs) and their $F_1$ hybrids with SNU-SG1. SNU-SG1 exhibited a typical characteristic of functional stay-green in terms of chlorophyll degradation and photosynthetic competence during grain filling. According to the photosynthesis-light response curve measured at 10 and 35 d after heading for the flag leaf, SNU-SG1 exhibited higher initial light conversion efficiency and thus higher gross photosynthetic rate at light saturation compared to HYVs. Light saturation point was not different among genotypes, ranging from 1000 to 1500 ${\mu}mol$ photon $m^{-2}s^{-1}$. Net photosynthetic rate at light saturation($P_{max}$) of the upper four leaves in SNU-SG1 was much higher and sustained longer throughout grain-filling than HYVs and $F_1$ hybrids. The sustained high photosynthetic competence of SNU-SG1 during grain filling was ascribed to the longer maintenance of high mesophyll conductance that resulted from not only high chlorophyll content and its delayed degradation but also the slow degeneration of photosystem II(PS II) as judged by chlorophyll fluorescence($F_v/F_m$) of flag leaves. $F_1$ hybrids showed slow degeneration of photosystem II similar to the male parent SNU-SG1 while chlorophyll degradation pattern close to female parents, thus exhibiting a little higher $P_{max}$ than female parents. These results suggest that SNU-SG1 has a typical functional stay-green trait that can be utilized for increasing rice yield potential through the improved dry matter production during grain filling.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.72-72
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• 2017

We found a new stay-green mutant from 'Pyeongwon' which is an early-maturing rice variety in Korea. The mutant showed green leaves after grain ripening period and it maintained higher SPAD value than wild type rice plant and original variety 'Pyeongwon'. The stay-green trait in rice, three genes have been identified up to date. The non-yellow coloring1 (NYC1) gene encodes a chloroplast-localized short-chain dehydrogenase/reductase (SDR) with three transmembrane domains. The non-yellow coloring3 (NYC3) gene encodes a plastid-localizing alpha/beta hydrolase-fold family protein with an esterase/lipase motif. The Sgr gene encodes a novel chloroplast protein and regulates the destabilization of the light-harvesting chlorophyll binding protein (LHCP) complexes of the thylakoid membranes, which is a prerequisite event for the degradation of chlorophylls and LHCPs during senescence. After sequencing the PCR products, we found a single nucleotide variation($A{\rightarrow}T$) in the NYC1 gene, which changes the amino acid lysine to methionine. The NYC1 gene encodes a short-chain dehydrogenase/reductase(SDR) protein. And we confirmed the co-segregation between SNP and stay-green trait from genotyping the progenies of the mutant.

• BMB Reports
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• v.33 no.3
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• pp.256-262
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• 2000

In this study the disassembly process of chlorophyII (ChI)protein complexes of a stay-green mutant (ore10 of Arabidopsis thaliana) was investigated during the dark incubation of detached leaves. During this dark-induced senescence (DIS), the Chi loss was delayed in the mutant, while the photochemical efficiency of photosystem II (PSII) or Fv/Fm was accelerated when compared with the wild type (WT) leaves. This indicates that the decrease in Fv/Fm is a separate process and not causally-linked to the degradation of Chi during DIS of Arabidopsis leaves. In the native green gel electrophoresis of the Chi-protein complexes, which was combined with an additional twodimensional SDS-PAGE analysis, the delayed senescence of this mutant was characterized by the appearance of an aggregate at 1 d or 2 d, as well as very stable light harvesting complex II (LHCII) trimers until 5 d after the start of DIS. The polypeptide composition of the aggregates varied during the whole DIS at 5 d. Dl protein appeared to be missing in the aggregates. This result supports the idea of a faster depletion of functional PSH in the mutants compared with WT, as suggested by the earlier reduction of Fv/Fm and the stable Chl a/b ratio in the mutants. At 5 d, the WT leaves also often showed aggregates, but the polypeptide composition was different from those of ore10. The results presented suggest that the formation of aggregates, or stable LHCII trimers in the stay-green mutants, is a way to structurally protect Chi-protein complexes from serious proteolytic degradation. Detailed disassembly processes of Chi-protein complexes in WT and ore10 mutants are discussed.

• Proceedings of the Korean Society of Agricultural Engineers Conference
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• 2003.10a
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• pp.87-90
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• 2003

This study aims to suggest a rural village development policy considering tourists' stay pattern and villages' resources which can be infrastructure for green-tourism. For this purpose, a relationship table between amenity resources and the stay pattern was developed as a guide line to combine a set of several facilities for village renewal. The amenity resources system was classified into three sub-classes(social, industrial, and natural/cultural/historical resources) and a relationship diagram between three classes and tourists' behavior was also defined. The table and diagram can be applied to make renewal plan for rural villages.

• Journal of Korean Society of Rural Planning
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• v.11 no.1 s.26
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• pp.45-53
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• 2005

This study aims to suggest a rural village development policy considering tourists' stay pattern and villages' resources which can be infrastructure for green-tourism. For this purpose, a relationship table between amenity resources and the stay pattern was developed as a guide line to combine a set of several facilities fer village renewal. The amenity resources system was classified into three sub-classes(social, industrial, and natural/cultural/historical resources) and a relationship diagram between three classes and tourists' behavior was also defined. The table and diagram were applied to make renewal plan for 40 rural villages of the study area, Bukmyun, Cheonan, Chungnam province, so that the development scheme for the villages was suggested reasonably by the new findings of this study.

• Proceedings of the Korean Society of Crop Science Conference
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• 1999.05a
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• pp.12-13
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• 1999

• Korean Journal of Organic Agriculture
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• v.15 no.2
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• pp.171-183
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• 2007

Green tourism is a stay-flow activity between people in urban and rural area, using a natural view, tradition, and life in agricultural area. And green tourism provides rest, recreation, and new experience fur people living in urban area as well as opportunities to increase income for people in rural area. In recently, amenity is rapidly rising as a main factor fur a regional competitiveness. Boseong county shows a typical agriculture industrial structure with a weak independent base, but has a beautiful tea fields in full seasons. Boseong county is making an effort to activate the regional economy, but the result fall short of one's expectation. Several methods to activate the green tourism of Boseong county are as follows: i) introduction of specialized theme relating Boseong green tea; ii) provision of information regarding tourist resort and development of experience-program related to green tea business; iii) establishment of a sales network of products through accommodations in private houses; iv) establishment of networks among regions to achieve systematic sales of tourist experience, accommodations in private houses, and local speciality simultaneously.

• Journal of Korean Society for Quality Management
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• v.38 no.4
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• pp.521-530
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• 2010

Global regulatory pressures relating climate change and environmental responsibility are asking companies to find out the best way for sustaining their continuous business growths. It could be known that inadequate management for environmental issues are bad for business, negatively affecting brand image, causing unnecessary losses and costs for environmental preservation. For this reason, environmentally conscious green business growth has been recognized as an essential requirement for a company to stay in business. Many companies are looking for green business opportunities of improving their environmental and financial results, and struggling with how green fits into their business. In this paper, the Green Six Sigma, an environmentally conscious Six Sigma methodology, is presented as a way to find solutions for green growths. The Six Sigma is known as a disciplined, data-driven approach and methodology for achieving world-class performance in any process from manufacturing to transactional. In chronological order, the Six Sigma has been evolved from Motorola's quality-oriented methodology to GE's cost-oriented lean approach, and is being evolved and developed as an environment-oriented green growth approach. There is no doubt that the Green Six Sigma, as an engine of green growth, is a power tool for achieving competitive business performance and reducing the impact on the environment.