• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2006년도 Proceedings of ISRS 2006 PORSEC Volume I
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• pp.29-33
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• 2006

The potential impact of past Caribbean tsunamis generated by earthquakes and/or massive submarine slides/slumps, as well as the tsunamigenic potential and population distribution within the Intra-Americas Sea (IAS) was examined to help define the optimal location for coastal sea level gauges intended to serve as elements of a regional tsunami warning system. The goal of this study was to identify the minimum number of sea level gauge locations to aid in tsunami detection and provide the most warning time to the largest number of people. We identified 12 initial, prioritized locations for coastal sea level gauge installation. Our study area approximately encompasses $7^{\circ}N$, $59^{\circ}W$ to $36^{\circ}N$, $98^{\circ}$ W. The results of this systematic approach to assess priority locations for coastal sea level gauges will assist in developing a tsunami warning system (TWS) for the IAS by the National Oceanic and Atmospheric Administration (NOAA) and the Intergovernmental Oceanographic Commission's Regional Sub-Commission for the Caribbean and Adjacent Regions (IOCARIBE-GOOS).

• Archives of Pharmacal Research
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• 제29권1호
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• pp.59-63
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• 2006

Secalonic acid D(SAD) was isolated as the major secondary metabolite of the marine lichen-derived fungus Gliocladium sp. T31. Its structure was established on the basic of physicochemical and spectroscopic data. This is the first report on the isolation of SAD from this fungus, as well as its inhibitory effect on K562 cell cycle and its cytotoxicity against several tumor cell lines in vitro.

• Journal of Microbiology and Biotechnology
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• 제19권9호
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• pp.873-880
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• 2009

A novel xylanase gene, Kxyn, was cloned from Kocuria sp. Mn22, a bacteria isolated from the deep sea of the east Pacific. Kxyn consists of 1,170 bp and encodes a protein of 390 amino acids that shows the highest identity (63%) with a xylanase from Thermohifida fusca YX. The mature protein with a molecular mass of approximately 40 kDa was expressed in Escherichia coli BL21 (DE3). The recombinant Kxyn displayed its maximum activity at $55^{\circ}C$ and at pH 8.5. The $K_m,\;V_{max}$, and $k_{cat}$ values of Kxyn for birchwood xylan were 5.4 mg/ml, $272{\mu}mol/min{\cdot}mg$, and 185.1/s, respectively. Kxyn hydrolyzed birchwood xylan to produce xylobiose and xylotriose as the predominant products. The activity of Kxyn was not affected by $Ca^{2+},\;Mg^{2+},\;Na^+,\;K^+$, ${\beta}$-mercaptoethanol, DTT, or SDS, but was strongly inhibited by $Hg^{2+},\;Cu^{2+},Zn^{2+}$, and $Pb^{2+}$. It was stable over a wide pH range, retaining more than 80% activity after overnight incubation at pH 7.5-12. Kxyn is a cellulase-free xylanase. Therefore, these properties make it a candidate for various industrial applications.

• Journal of Microbiology and Biotechnology
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• 제19권10호
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• pp.1077-1084
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• 2009

A genomic library was constructed to clone a xylanase gene (Mxyn10) from Demequina sp. JK4 isolated from a deep sea. Mxyn10 encoded a 471 residue protein with a calculated molecular mass of 49 kDa. This protein showed the highest sequence identity (70%) with the xylanase from Streptomyces lividans. Mxyn10 contains a catalytic domain that belongs to the glycoside hydrolase family 10 (GH10) and a carbohydrate-binding module (CBM) belonging to family 2. The optimum pH and temperature for enzymatic activity were pH 5.5 and $55^{\circ}C$, respectively. Mxyn10 exhibited good pH stability, remaining stable after treatment with buffers ranging from pH 3.5 to 10.0. The protein was not significantly affected by a variety of chemical reagents, including some compounds that usually inhibit the activity of other related enzymes. In addition, Mxyn10 showed activity on cellulose. These properties mark Mxyn10 as a potential enzyme for industrial application and saccharification processes essential for bioethanol production.

• 한국대기환경학회지
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• 제34권2호
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• pp.342-355
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• 2018

Emission inventory (EI) is often recognized as a foundational pillar for air quality planning. In this manuscript, we reviewed national emission inventory (NEI) development processes in United States(US) and made recommendations for South Korean emission inventory development for the future. First, we examined history of emission inventory development in US while focusing on what has been success and failure. We noticed that, in general, emission reductions led to air pollution abatement although efficacy of same degree of reduction may not result in similar air quality improvement. Second, we described conventional approaches of developing NEI and differences between NEI and EI for State Implementation Plan which is required for air quality management in US. Last, we analyzed how US Environmental Protection Agency and counterpart agencies in states came up with a new plan for the next major regulatory modeling project. Based on our analysis, we conclude that early and steady participation of local governments will lead to effective and efficient emission inventory development and, in turn, will result in successful air quality planning that is necessary for actual air quality improvement.

• Journal of Microbiology and Biotechnology
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• 제26권12호
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• pp.2127-2137
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• 2016

A mangrove microbial community was analyzed at the gene and protein levels using metagenomic and proteomic methods with the green macroalgae Enteromorpha prolifera as the substrate. Total DNA was sequenced on the Illumina HiSeq 2000 PE-100 platform. Two-dimensional gel electrophoresis in combination with liquid chromatography tandem mass spectrometry was used for proteomic analysis. The metagenomic data revealed that the orders Pseudomonadales, Rhizobiales, and Sphingomonadales were the most prevalent in the mangrove microbial community. By monitoring changes at the functional level, proteomic analyses detected ATP synthase and transporter proteins, which were expressed mainly by members of the phyla Proteobacteria and Bacteroidetes. Members of the phylum Proteobacteria expressed a high number of sugar transporters and demonstrated specialized and efficient digestion of various glycans. A few glycoside hydrolases were detected in members of the phylum Firmicutes, which appeared to be the main cellulose-degrading bacteria. This is the first report of multiple "omics" analysis of E. prolifera degradation. These results support the fact that key enzymes of glycoside hydrolase family were expressed in large quantities, indicating the high metabolic activity of the community.

• 한국대기환경학회지
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• 제34권4호
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• pp.588-609
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• 2018

The objective of this study is to provide information for improving the domestic particulate matter (PM) management. To achieve the study goal, we reviewed the past and current practice of PM management in the United States (US). Our review includes the assessment about the philosophical foundation and effectiveness of PM management, systematic program implementation to reach desirable PM conditions, and steady development of analytical tools including photochemical air quality models. We noticed that the current US PM management approach has improved annual $PM_{2.5}$ concentrations 22-48% in various parts of the US in the last 16 years. In addition, we analyzed strengths and difficulties in the past US PM management implementation. Based on the results of our analyses, we propose key elements to establish more effective domestic PM management in the future than now: steady investment in technological innovations and close-communications between local governments and the central government at various stages of PM management.

• 한국지구과학회지
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• 제33권6호
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• pp.554-566
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• 2012

성공적인 국제 Argo 사업을 통해 얻어진 고해상도의 수온 및 염분 프로파일들을 이용하여 준실시간으로 3차원 전지구 해양 상태의 재현이 가능해졌으며, 전지구 해양 자료 분석 연구에 대한 관심 또한 크게 증가하였다. 본 연구에서는 XBT와 Argo 자료의 계통 오차, 열염분 해수면 높이 및 인공위성 관측 자료와의 해수면 수지 불일치, 해양 열용량 변동 및 객관 분석장 개발 현황 등 최근의 자료 분석 연구의 주요 쟁점들에 관해 정리하였다. 특히 동아시아 해역에서의 지역 규모 해양 기후 변화 분석에 앞서 매우 신중한 해양 자료의 품질 관리 및 전구 규모의 해양 변동에 관한 이해가 필요하다는 것을 강조하였다.

• 한국해양학회지:바다
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• 제10권1호
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• pp.69-78
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• 2005

본 연구에서는 전구 해양에서 관측되는 ARGO및 TAO해양 자료를 이용하여 해양의 3차원적인 구조를 분석.동화하고 궁극적으로 해양대순환모형을 위한 초기장을 생산하였다. 초기장의 생산을 위하여 전구 해양대순환 모형인 MOM3.1을 이용하였으며 생산한 배경장에, 계산시간과 계산공간을 절약할 수 있는 공간필터를 사용한 변분법(VAF, variational analysis using filter)을 이용하여 ARGO와 TAO 수온 자료를 동화하였다. 또한 본 연구에서는 자료 동화가 미치는 지속적인 영향을 살펴보고자 실험적분을 수행하였는데, 모형의 초기입력 자료를 자료동화 기법을 적용한 경우와 적용하지 않은 두 가지로 나누어 비교 실험을 수행하였다. 본 연구에서 자료 동화된 분석장은 OISST와의 비교를 통해 적절히 생산되었음을 보여주었다. 관측자료를 동화한 분석장을 초기자료로 한 10개월간의 적분결과를 살펴보면, 자료 동화를 통해 제거된 모형의 계통적 bias가 적분이 진행되는 과정에서 관성 중력파 등의 형태로 소멸되지 않고 지속적으로 관측과 유사하게 유지되었다. 이는 본 연구에서 실행한 자료동화가 모형의 역학적인 균형을 유지하면서 적절히 이루어졌음을 의미하며, 전구 대순환 모형을 이용한 중.장기 대기.해양 예측에 이러한 해양 자료동화가 대단히 유용하다는 것을 의미한다.

• 한국지하수토양환경학회지:지하수토양환경
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• 제12권4호
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• pp.16-24
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• 2007

시화호 저서생태계의 비용효과적 복원을 위한 퇴적토 관리 대안을 제시하기 위하여 시화호 내 오염퇴적물의 현황 및 저서생태계 특성에 대한 조사와 퇴적물 관리 기준과 사례 연구가 수행되었다. 미국해양대기청의 퇴적물환경권고기준에 따라 시화호 퇴적토의 중금속 오염도를 평가한 결과 13개 조사지점 중 반월/시화 산단과 인접해 있는 4개 지점의 퇴적토가 2개 이상의 중금속에서 ERL (Effects Range Low : 저서생물 10%에 악영향을 끼칠 수 있는 농도)을 초과하는 것으로 나타났다. 그러나 미국 워싱턴 주와 네덜란드에서 정하고 있는 퇴적토 제거기준은 초과하지 않는 것으로 나타났다. 반면에 유기물 및 황화물 오염의 관점에서 개발된 일본 나고야항과 동경만/요코하마만의 퇴적물 제거기준에 의하면 시화호 표층 퇴적물 대부분이 제거대상인 것으로 나타났다. 한편 저서생물의 조사결과 Polydora ligni (얼굴갯지렁이)와 Musculista senhousia (종밋)와 같은 오염지시종이 우점종으로 출현하였으며, 이로 미루어 유기물 오염에 의한 여름철 용존산소 고갈 및 황화수소 생성등이 일반적인 저서생물의 건강한 성장을 크게 저해하는 것으로 판단되었다. 유기물, 황화물 및 중금속과 오염퇴적토가 주변지역에 미치는 영향을 종합적으로 고려한 결과 일부 지역은 우선적으로 퇴적토 정화가 이루어져야 할 것으로 판단되었다.