• 제목/요약/키워드: Staphylococcus sp.

검색결과 208건 처리시간 0.022초

기포 발생형 타정제의 살균세정 효과 (Efficacy of Disinfectants and Sanitizers of Chlorine Oxide Bubbling Tablets)

  • 지원대;강상구;이경은
    • 생명과학회지
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    • 제25권9호
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    • pp.1036-1042
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    • 2015
  • 병원균의 조기 차단을 목적으로 본 연구에서는 사용이 간편한 chlorine oxide 기포 발생형 살균세정용 타블렛 정제의 살균효과를 검증하였다. 대한민국 식품의약품안전처 고시 2012-10호의 사용기준에 따른 살균력의 검증결과, 본 연구에서 사용한 살균세정제를 20±1℃에서 5분±10초 처리하였을 때 Escherichia coli ATCC 10536 및 Staphylococcus aureus ATCC 6538의 균에 대하여 초기균수(cfu/ml)를 99.999% 이상 감소하였다. 살균세정제를 희석 농도 0.1%의 경수에 1일 사용한 양말과 상하 내의를 1시간 침지한 후 CHROMagar 배지를 사용하여 살균정도를 조사한 결과 살균세정제가 투여된 처리구에서는 균이 전혀 검출되지 않았으나 무 처리 대조군의 경우 Staphylococcus sp. 등을 포함한 총 6.5×104 cfu/ml의 균체가 자랐다. 또한 1.5×108 cfu/ml의 실험용 E. coli BL21 균주에 살균세정제 0.1% (w/v)을 투여한 결과 투여 5분 내에 모든 균이 사멸하였다. 본 연구에서 검증한 기포발생형 살균세정제는 병원균, 실험용 대장균 및 생활용품에 대하여 뛰어난 살균효과가 있는 것으로 판명되었다.

주택내 주거공간에 따른 부유세균 농도 분포 및 종 동정 연구 (Airborne Bacteria Concentration and Species Identification in Residential Living Spaces)

  • 김성연;정원화;황은설;김지혜;정준식;이재원;정현미;권명희
    • 한국환경보건학회지
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    • 제42권6호
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    • pp.438-449
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    • 2016
  • Objectives: Exposure to airborne bacteria is associated with adverse health effects such as respiratory and infectious diseases. This study evaluated airborne bacterial concentrations in the living rooms, kitchens, and toilets of 30 homes. Methods: Bacteria were sampled with an MAS100 impactor in three spaces in the subject homes between April 2014 and February 2015. Bacteria were grown on TSA plates for 48 hours at $35^{\circ}C$. The bacterial strains were isolated and amplified by polymerase chain reaction. Results: The most culturable bacteria were found in toilets ($624.0CFU/m^3$, GM: $417.3CFU/m^3$), followed by in the kitchen ($503.8CFU/m^3$, GM: $324.9CFU/m^3$). The dominant genera identified were: Staphylococcus sp.(19%), Micrococcus sp.(16%), and Bacillus sp.(11%) in the indoor air and Bacillus sp. (30%) in the outdoor air. Gram-positive bacteria comprised more than half of all colonies. Conclusion: In this study, culturable bacteria concentrations were higher than those reported in other spaces. Therefore, it is important to control relative humidity and remove moisture to prevent bacteria from multiplying. Additionally, the dominant species in indoor air were Staphylococcus sp. and Micrococcus sp. These are found on the human skin, mucous membranes, and hair, so human activity can affect bacterial distribution. Therefore, cleaning and controlling moisture are important for reducing indoor bacterial concentrations.

곤충병원세균(Xenorhabdus nematophila) 유래물질의 톱다리개미허리노린재(Riptortus clavatus) 장내세균 증식억제 및 살충효과 (Antibiotic and Insecticidal Activities of Metabolites Derived From an Entomopathogenic Bacterium, Xenorhabdus nematophila, Against The Bean Bug, Riptortus clavatus)

  • 서삼열;김용균
    • 한국응용곤충학회지
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    • 제49권3호
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    • pp.251-259
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    • 2010
  • 톱다리개미허리노린재(Riptortus clavatus)의 장내세균이 분리되었다. 형태학적 분석과 생화학적 분석을 통하여 세균이 Staphylococcus succinus와 가장 유사한 것으로 동정되었다. 16S rRNA 유전자의 염기서열은 이러한 동정 결과를 뒷받침했다. 페니실린G를 톱다리개미허리노린재 성충에게 경구투여 하였을 때 장내세균 밀도 감소와 치사 효과를 유발하였다. 동일한 방법으로 곤충병원세균(Xenorhabdus nematophila)의 세 가지 대사물질(benzylideneacetone, proline-tyrosine, and acetylated phenylalanine-glycine-valine)을 처리하였을 때, 톱다리개미허리노린재 장내세균의 밀도감소와 치사효과를 확인하였다. 이러한 결과는 톱다리개미허리노린재의 장내세균이 Staphylococcus sp.이며, 곤충병원세균 대사물질의 항균 활성이 장내세균과 궁극적으로 톱다리개미허리노린재의 생존에 영향을 미친다는 것을 제시하였다.

Bioluminescence 반응에 의한 ATP측정을 이용한 젖소 유방염 치료에 관한 연구 (Treatment test for bovine mastitis by the determination of ATP based on firefly bioluminescence)

  • 김태종;김종배;이승배;전용수
    • 대한수의학회지
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    • 제29권3호
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    • pp.393-405
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    • 1989
  • This study was carried out to treatment test for bovine mastitis by the determination of adenosine triphosphate (ATP) based on firefly bioluminescence. The results obtained are followed; 1. In the susceptibility test, cephalothin which looks the most effective were sensitive to Staphylococcus sp. (72.3%), Micrococcus sp. (84.2%), Streptococcus sp. (72.7%) and Gram positive bacilli (72.7%), Gram negative bacilli were sensitive to gentamicin (92.3%) and Yeast-like-fungi was the most sensitive to clotrimazole, and nystatin in order. 2. When the number of bacteria, such as Staphylococcus aureus, Candida tropicalis isolated from the mastitis milk were counted by conventional agar plating technique, and compared with the concentration of bacterial ATP, it gave a good linear relationship. The content of ATP per Staphylococcus aureus, cell was 3.1fM and Candida tropicalis showed the high level of A TP (90fM). 3. The ATP assay was applied to the determination of minimal inhibitory concentration (MIC) of various antibiotics. When Staphylococcus aureus was incubated in the presence of different concentration of tetracycline, erythromycin, kanamycin and streptomycin sulfate and the growth was monitored by the conventional agar plating technique and ATP assay, both methods shown the same results that they were 1mcg/ml, 2mcg/ml, 6.25mcg/ml and 8mcg/ml, respectively. 4. For the determination of susceptibility of sensitive and resistant Staphylococcus au reus isolated for the milk with mastitis to tetracycline, erythromycin kanamycin and streptomycin sulfate, the minimum time required for the test was determined by the assay of ATP every 30 minutes during incubation of 3 hours at $37^{\circ}C$. ATP concentration time curve calculated on both resistant and sensitive strains incubated 3 hours as the optimum time for the determination of susceptibilities of various antibiotics exemed. The ATP concentration of each test broth (antibiotic containing), expressed as a percentage of its own control brith (antibioticfree) indicated values of 30% to be indicative of each antibiotic sensitivity. Single time point ATP assay carried out on the various sensitive and resistant of Staphylococcus aureus to antibiotics examined after 3 hours at $37^{\circ}C$ correlated exactly with disc diffusion and MIC. 5. In the cure of intramammary treatment of bovine mastitis in lactating quarters, the cure rate of Staphylococcal mastitis showed to cephalexin (80%), cloxacillin and gentamicin (70%), ampicillin and oxytetracycline (60%), and Streptococcal mastitis showed to cephalexin (85%), penicillin (80%), cloxacillin and oxytetracycline (75%), and ampicillin (70%), but intramammary antimycotic drug (clotrimazol) were only a little effect about fungal mastitis.

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도태유우(淘汰乳牛)에 있어서의 유방염(乳房炎)에 관(關)한 연구(硏究) 제(第)1보(報) 유방감염(乳房感染) (Mastitis in Slaughtered Dairy Cows 1. Udder Infection)

  • 이채용;강병규;박영준;이정길
    • 대한수의학회지
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    • 제20권2호
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    • pp.119-122
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    • 1980
  • The udders of 28 culled dairy cows were removed at slaughter and bacteria isolated. Isolations of major known mammary pathogens comparised Staphylococcus sp. (40.7%), non-identified Gram's negative rods (33.2%) and E. coli (7.4%). In the mixed infection, Staphylococcus sp. was most frequently involved. Drug sensitivity tests were performed on several bacterial species by disc method. These tests indicated that the microorganisms isolated from clinical mastitis of dairy cows were more resistant to antimicrobial agents than the organisrms isolated from milk samples.

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Bacterial Diversity in a Korean Traditional Soybean Fermented Foods (Doenjang and Ganjang) by 16S rRNA Gene Sequence Analysis

  • Cho, Kye-Man;Seo, Weon-Taek
    • Food Science and Biotechnology
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    • 제16권2호
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    • pp.320-324
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    • 2007
  • The bacterial diversity in Korean soybean-fermented foods was investigated using a PCR-based approach. 16S rRNA sequences were amplified and cloned from two different soybean-fermented foods such as doenjang (soybean paste), and ganjang (soybean sauce). Staphylococcus equorum (60.6%), Tetragenococcus halophila (21.2%), Leuconostoc mesenteroides (9.1%), Lactobacillus sakei (6.1%), and Bacillus subtilis (3.0%) were detected among clones isolated from soybean paste samples and Halanaerobium sp. (37.5%), Halanaerobium fermentans (37.5%), T. halophila (12.5%), Staphylococcus sp. (6.3%), S. equorum (3.1%), and B. subtilis (3.1%) were detected among clones isolated from soybean sauce. Our approach revealed different bacterial distributions and diversity from those previously obtained using culture-dependent methods.

오존처리법에 의한 양어용수 살균에 대하여 I. 해산어류 병원세균의 오존 감수성 (Disinfection of Culture Water Supply by Ozonization I. Susceptibility of Some Fish-Pathogenic Bacteria Isolated From Cultured Marine Fish)

  • 오명주;김흥윤;조현서
    • 한국어병학회지
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    • 제12권1호
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    • pp.42-48
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    • 1999
  • 해수중 잔류옥시던트(TRO) 농도를 0.1, 0.3 및 0.5 mg/liter로 조정하고 시간 경과에 따른 농도의 안정성을 관찰한 결과 0.1 mg/liter의 경우 측정 20분까지는 변화를 보이지 않았고, 0.3 및 0.5 mg/liter 에서는 1시간 경과시까지 안정하였다. 해수를 오존처리하여 해수중에 생성되는 TRO를 이용하여 해산어류 병원세균인 Edwardsiella tarda, Vibrio sp., Streptococcus sp., Staphylococcus sp.에 대한 살균효과를 실험한 결과 0.1 mg/liter에서 3분간의 처리로 99.9% 이상, 0.3~0.5 mg/liter에서는 30초에서 1분간의 반응시간으로 99.99% 이상이 살균되어졌다.

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$\beta$-Lactamase 저해능이 있는 방선균의 선별 (Screening of Microorganisms Having Inhibitory Activity against $\beta$-lactamase)

  • 강희일;김영일;박영주
    • 약학회지
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    • 제28권2호
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    • pp.89-95
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    • 1984
  • Microorganisms having beta-latamase inhibitory activity were selected from soil samples collected from 63 spots throughout the country. Screening procedures consist of two steps. Those are growth inhibition test of penicillinase-producing Staphylococcus aureus by double-layered agar plate containing penicillin G as a substrate, and that of penicillin sensitive Staphylococcus aureus ATCC 6538 in the similiar condition including penicillinase. Finally, a strain was selected from a soil sample of Pa-ju, Kyeong-gi Do. This strain was classified as a Streptomyces sp. by ISP(International Streptomycete Project) and Bergey's manual.

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Microbiological Quality Assessment of a Local Milk Product, Kwacha Golla, of Bangladesh

  • Rahman, M.M.;Rahman, M.Mashiar;Arafat, S.M.;Rahman, Atiqur;Khan, M.Z.H.;Rahman, M.S.
    • Applied Biological Chemistry
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    • 제51권4호
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    • pp.251-257
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    • 2008
  • Different types of milk products, such as kwacha golla, mawa, cheese, curd, and chocolate are popular in Bangladesh. However, the microbiological safety of these products is poorly understood. This study was performed to assess the microbiological quality of kwacha golla, a local milk product. Kwacha golla samples were collected from ten different areas of Rajshahi and Kushtia regions, and the quality of the each sample was assessed using various parameters including standard plate count, total coliform, fecal coliform, total fungi, and spore-forming bacteria, as well as food-borne microorganisms. Out of 300 samples, total coliform was detected at 56.66% (n= 300), exceeding the minimum allowable limit of 36.66%. Similarly, experiments were carried out with fungi and food-borne pathogens including Escherichia coli, Listeria monocytogenes, Salmonella sp., and Staphylococcus aureus. Results revealed 85.33, 53, and 49.33% of the samples were contaminated by fungi, E. coli, and L. monocytogenes, respectively. However, all samples showed no contaminations of Salmonella sp. and Staphylococcus sp. Therefore, this study could be helpful to the people of Bangladesh by providing information on the possibility of a major health problem caused by the consumption of kwacha golla.

Agelas속의 미동정 해면으로부터 항말라리아 활성을 갖는 Dibromosceptrin의 분리 (Isolation of Dibromosceptrin with Antimalarial Activity from the Unidentified Sponge, Agelas sp.)

  • 박영범;이종수;임치원
    • 생약학회지
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    • 제35권3호통권138호
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    • pp.189-193
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    • 2004
  • In order to find some lead compounds for the treatment of opportunistic infections of malaria and pathogenic microbes, an undescribed Indonesian sponge Agelas sp. collected at Manado, Indonesian Waters, was suggested containing active compounds. Crude ethanolic extract of the sponge exhibited significant in vitro antimalarial and antimicrobial activity against Plasmodium falciparum (D6 colne) with $IC_{5O}$ values of $8\;{\mu}/ml$ and against pathogenic microbes such as Candida albicans $(150\;{\mu}/ml)$, Cryptococcus neoformans $(<20\;{\mu}/ml)$, Staphylococcus aureus $25\;{\mu}/ml$, methicillin-resistant Staphylococcus aureus $(<20\;{\mu}/ml)$, and Pseudomonas aeruginosa $(<20\;{\mu}/ml)$. Active compound (5.0 mg) was isolated from the ethanolic extracts of the sponge and purified by using silica gel and ODS column, successively. Active compound was elucidated as dibromosceptrin $(C_{22}H_{24}Br_2N_{10}O_2)$ by detailed analysis of FTESI-MS and comparison of $^1H,\;^{13}C$, DEPT and HMQC NMR spectral data with those reported.