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http://dx.doi.org/10.5656/KSAE.2010.49.3.251

Antibiotic and Insecticidal Activities of Metabolites Derived From an Entomopathogenic Bacterium, Xenorhabdus nematophila, Against The Bean Bug, Riptortus clavatus  

Seo, Sam-Yeol (Department of Bioresource Sciences, Andong National University)
Kim, Yong-Gyun (Department of Bioresource Sciences, Andong National University)
Publication Information
Korean journal of applied entomology / v.49, no.3, 2010 , pp. 251-259 More about this Journal
Abstract
A bacterial colony was isolated from the gut of the bean bug, Riptortus clavatus. From morphological and biochemical tests, the bacterial isolate showed the highest similarity to Staphylococcus succinus. DNA sequence of 16S rRNA gene of the bacterium supported the identification. Oral administration of penicillin G to adults of R. clavatus gave a dose-dependent mortality of adults of R. clavatus to adults along with significant decrease of the bacterial population in the gut. Similarly, three metabolites (benzylideneacetone, proline-tyrosine, and acetylated phenylalanine-glycine-valine) derived from an entomopathogenic bacterium, Xenorhabdus nematophila, also inhibited growth of the gut bacterial population and gave significant mortalities to R. clavatus. These results suggest that a gut bacterial population classified as Staphylococcus sp. is required for survival of R. clavatus and that the three bacterial metabolites had toxic effects on the bugs due to their antibacterial properties.
Keywords
benzylideneacetone; Xenorhabdus nematophila; Staphylococcus; 16S rRNA; Riptortus clavatus;
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1 Stanley, D.W. and J.S. Miller. 2006. Eicosanoid actions in insect cellular immune functions. Entomol. Exp. Appl. 119: 1-13.   DOI
2 Stanley, D. 2006. Prostaglandins and other eicosanoids in insects: biological significance. Aunu. Rev. Entomol. 51: 25-44.   DOI
3 Kikuchi, Y., T. Hosokawa and T. Fukatsu. 2007. Insect-microbe mutualism without a vertical transmission: a stinkbug acquires a beneficial gut symbiont from the envrronment every generation. Appl. Environ. Microbiol. 73: 4308-4316.   DOI
4 Kikuchi, Y., X.Y. Meng and T. Fukatsu. 2005. Gut symbiotic bacteria of the genus Burkholderia in the broad-headed bugs Riptortus clavatus and Leptocorisa shinensis (Heteroptera: Alydidae). Appl. Environ. Microbiol. 71: 4035-4043.   DOI
5 Valiente Moro, C., J. Thioulouse, C. Chauve, P. Normand and L. Zenner. 2009. Bacterial taxa associated with the hematophagous mite Dermanyssus gallinae detected by 16S rRNA PCR amplification and TTGE fingerprinting. Res. Microbiol. 160: 63-70.   DOI
6 Ji, D., Y. Yi, G.H. Kang, Y.H. Choi, P. Kim, N.I. Baek and y. Kim. 2004. Identification of an antibacterial compound, benzylideneacetone, from Xenorhabdus nematophila against major plant-pathogenic bacteria. FEMS Microbiol. Lett. 239: 241-248.   DOI
7 Kanost, M.R., H. Jiang and X. Yu. 2004. Innate immune responses of a lepidopteran insects, Manduca sexta. Immunol. Rev. 198: 97-105.   DOI
8 Kaya, H.K. and R. Gaugler. 1993. Entomopathogenic nematodes. Annu. Rev. Entomol. 38: 181-206.   DOI
9 Kwon, S. and Y. Kim. 2008. Benzylideneacetone, an immunosuppressant, enhances virulence of Bacillus thuringiensis against beet armyworm (Lepidoptera: Noctuidae). J. Econ. Entomol. 101: 36-41.   DOI
10 Lee, K.C., C.H. Kang, D.W. Lee, S.M. Lee, C.G. Park and H.Y. Choo. 2002. Seasonal occurrence trends of hemipteran bug pests monitored by mercury light and aggregation pheromone traps in sweet persimmon orchards. Kor. J. Appl. Entomol. 41: 233-238.   과학기술학회마을
11 Li, J., G. Chen, H. Wu and J.M. Webster. 1995. Identification of two pigments and a hydroxystilbene antibiotic from Photorhabdus luminescens. Appl. Environ. Microbiol. 61: 4329-4333.
12 Park, Y., and Y. Kim. 2000. Eicosanoids rescue Spodoptera exigua infected with Xenorhabdus nematophila, the symbiotic bacteria to the entomopathogenic nematode Steinernema carpocapsae. J. Insect Physiol. 46: 1469-1476.
13 Park, Y., M. Kim, G. Lee, W. Chun, Y. Lee and Y. Kim. 2009. Inhibitory effects of an eicosanoid biosynthesis inhibitor, benzylideneacetone, against two spotted spider mite, Tetranychus urticae, and a bacterial wilt-causing pathogen, Ralstona solanacearum. Kor. J. Appl. Entomol. 13: 185-189.
14 Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular nd cloning. A laboratory manual. 2nd ed. Cold Spring Harbour Society. 164 pp. St. Paul, MN, USA.
15 Bae, S.D., H.J. Kim, G.H. Lee and S.T. Park. 2008. Development of observation methods for density of stink bugs in soybean field. Kor. J. Appl. Entomol. 46: 153-158.   과학기술학회마을   DOI
16 Chung, B.K., S.W. Kang and J.H. Kwon. 1995. Damages, occurrences and coutrol of hemipterousinsects in non-astringent persimmon orchards. RDA. J. Agri. Sci. 37: 376-382.
17 Dunphy, G.B. and J.M. Webster. 1991. Antihemocytic surface components of Xenorhabdus nematophilus var. dutki and their modification by Serum of nonimmune larvae of Galleria mellonella. J. lnvertebr. Pathol. 58: 40-51.   DOI
18 fftench-Constant, R.H., N. Waterfield and P. Daborn. 2005. Insecticidal toxins from Photorhabdus and Xenorhabdus. pp. 239-253, In Comprehensive molecular insect science, eds. by L.I. Gilbert, I. Kostas and S.S. Gill. Elsevier, New York.
19 Dunphy, G.B. and J.M. Webster. 1994. Interaction of Xenorhabdus nematophila subsp. nematophilus with the haemolymph of Galleria mellonella. J. Insect Physiol. 30: 883-889.
20 Eutick, M.L., R.W. O'Brien and M. Slaytor. 1978. Bacteria from the gut of Australian termites. Appl. Environ. Microbiol. 35: 823-828.
21 Forst, S. B. Dedos, N. Boemare and E. Stackebrandt. 1997. Xenorhabdus and Photorhabdus spp.: bugs that kill bugs. Annu. Rev. Microbiol. 51: 47-72.   DOI
22 Ha, K.S. 2004. Damages of hemipterous insects on major cultivated soybean cultivars in Gangwon province. Kor. Soyb. Digest 21: 1-5.
23 Hu, H.S., W. Huh, S.D. Bae and C.G. Park. 2005. Seasonal occurrence and ovarian development of bean bug, Riptortus clavatus. Kor. J. Appl. Entomol. 44: 199-205.   과학기술학회마을
24 Bae, S.D., H.J. Kim, C.G. Park, G.H. Lee, S.T. Park and Y.H. Song. 2005a. Reproductive rate of one-banded stink bug, Piezodorus hybneri Linnaeus (Hemiptera: Pentatomidae) in various rearing cages. Kor. J. Appl. Entomol. 44: 293-298.   과학기술학회마을
25 Adams, B.J. and K.B. Nguyen. 2002. Taxonomy and systematics. pp. 1-33. In Entomopathogenic nematology, ed. by R. Gaugler. CABI Publishing, New York.
26 Akhurst, R J. 1980. Morphological and functional dimorphism in Xenorhabdus spp., bacteria symbiotically associated with the insect pathogenic nematodes Neoaplectana and Heterorhabditis. J. Gen. Microbiol. 121: 303-309.
27 Bae, S.D., H.J. Kim, J.K. Park, J.K. Jung and H.J. Cho. 2004. Effects of food combinations of leguminous seeds on nymphal development, adult longevity and oviposition of bean bug, Riptortus clavatus Thunberg. Kor. J. Appl. Entomol. 43: 123-127.   과학기술학회마을
28 Bae, S.D., H.J. Kim, C.G. Park, G.H. Lee and S.T. Park. 2005b. The development and oviposition of bean bug, Riptortus clavatus Thunberg (Hemiptera: Alydidae) at temperature condi tions. Kor. J. Appl. Entomol. 44: 325-330.
29 SAS Institute, Inc. 1989. SAS/STAT user's guide, Release 6.03, Ed. Cary, N.C.
30 van der Hoeven, R., G. Betrabet and S. Forst. 2008. Characterization of the gut bacterial community in Manduca sexta and effect of antibiotics on bacterial diversity and nematode reproduction. FEMS Microbiol. Lett. 286: 249-256.   DOI
31 Shrestha, S. and Y. Kim 2008. Eicosanoids mediate prophenoloxidase release from oenocytoids in the beet armyworm Spodoptera exigua. Insect Biochem. Mol. Biol. 38: 99-112.   DOI
32 Shrestha, S. and Y. Kim. 2009. Biochemical characteristics of immune-associated phospholipase $A_{2}$ and its inhibition by an entomopathogenic bacterium, Xenorhabdus nematophila. J. Microbiol. 47: 774-782.   DOI
33 Son, C.K., S.G. Park, Y.H. Hwang and B.S. Choi. 2000. Field occurrence of stink bug and its damage in soybean. Kor. J. Crop Sci. 45: 405-410.   과학기술학회마을