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http://dx.doi.org/10.5668/JEHS.2016.42.6.438

Airborne Bacteria Concentration and Species Identification in Residential Living Spaces  

Kim, Sung-Yeon (Indoor Environment and Noise Research Division, National Institute of Environmental Research)
Jheong, Weonhwa (Environmental Health Research Division, National Institute of Environmental Research)
Hwang, Eun-Seol (Indoor Environment and Noise Research Division, National Institute of Environmental Research)
Kim, Ji-Hye (Water Supply & Sewerage Research Division, National Institute of Environmental Research)
Jung, Joon-Sig (Indoor Environment and Noise Research Division, National Institute of Environmental Research)
Lee, Jae-won (Indoor Environment and Noise Research Division, National Institute of Environmental Research)
Chung, Hyen-Mi (Indoor Environment and Noise Research Division, National Institute of Environmental Research)
Kwon, Myunghee (Indoor Environment and Noise Research Division, National Institute of Environmental Research)
Publication Information
Journal of Environmental Health Sciences / v.42, no.6, 2016 , pp. 438-449 More about this Journal
Abstract
Objectives: Exposure to airborne bacteria is associated with adverse health effects such as respiratory and infectious diseases. This study evaluated airborne bacterial concentrations in the living rooms, kitchens, and toilets of 30 homes. Methods: Bacteria were sampled with an MAS100 impactor in three spaces in the subject homes between April 2014 and February 2015. Bacteria were grown on TSA plates for 48 hours at $35^{\circ}C$. The bacterial strains were isolated and amplified by polymerase chain reaction. Results: The most culturable bacteria were found in toilets ($624.0CFU/m^3$, GM: $417.3CFU/m^3$), followed by in the kitchen ($503.8CFU/m^3$, GM: $324.9CFU/m^3$). The dominant genera identified were: Staphylococcus sp.(19%), Micrococcus sp.(16%), and Bacillus sp.(11%) in the indoor air and Bacillus sp. (30%) in the outdoor air. Gram-positive bacteria comprised more than half of all colonies. Conclusion: In this study, culturable bacteria concentrations were higher than those reported in other spaces. Therefore, it is important to control relative humidity and remove moisture to prevent bacteria from multiplying. Additionally, the dominant species in indoor air were Staphylococcus sp. and Micrococcus sp. These are found on the human skin, mucous membranes, and hair, so human activity can affect bacterial distribution. Therefore, cleaning and controlling moisture are important for reducing indoor bacterial concentrations.
Keywords
Airborne bacteria; Micrococcus sp.; residence; species; Staphylococcus sp.;
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1 Balasubramanian R, Nainar P, Rajasekar A. Airborne bacteria, fungi, and endotoxin levels in residential microenvironments : a case study. Aerobiologia. 2012; 28(3): 375-390.   DOI
2 Moon KW, Huh EH, Jeong HC. Seasonal evaluation of bioaerosols from indoor air of residential apartments within the metropolitan area in South Korea. Environ Monit Assess. 2014; 186(4): 2111-2120.   DOI
3 Kim MK. Determinations of total culturable bacteria in the university spaces. Korea Society of Environmental Administration. 2014; 20(2): 43-48.
4 Li Y, Leung M, Tang JW, Yang X, Chao CYH, Lin JZ, et al. Role of ventilation in airborne transmission of infectious agents in the built environment - a multidisciplinary systematic review. Indoor Air. 2007; 17(1): 2-18.   DOI
5 Seitz TA. NIOSH indoor air quality investigations : Proceedings of the Indoor Air Quality International Symposium, Cincinnati, OH, NIOSH, 1989.
6 Malgorzata GS, Gorny RL. Bacterial and fungal aerosols in air-conditioned office buildings in warsaw, poland-the winter season. Int J Occup Saf Ergon. 2010; 16(4): 465-476.   DOI
7 Pastuszka JS, Kyaw Tha Paw U, Lis DO, Wlaslo A, Ulfig K. Bacterial and fungal aerosol in indoor environment in Upper Silesia, Poland. Atmos Environ. 2000; 34(22): 3833-384.   DOI
8 Kim YS, Roh YM, Lee CM, Kim KY, Kim JC, Jeon HJ, et al. A study of excess ratio for guideline of indoor air pollutants in classroom of kindergartens. J Korean Soc Indoor Environ. 2007; 4(1): 14-22.
9 Ferro AR, Kopperud RJ, Hildemann LM. Source strengths for indoor human activities that resuspend particulate matter. Environ Sci Technol. 2004; 38: 1759-1764.   DOI
10 Koistinen KJ, Edwards RD, Mathys P, Ruuskanen J, Kunzli N, et al. Sources of fine particulate matter in personal exposures and residential indoor, residential outdoor and workplace microenvironments in the Helsinki phase of the EXPOLIS study. Scand J Work Env Hea. 2004; 30: 36-46.   DOI
11 Koperrud RJ, Ferro AR, Hildemann LM. Outdoor versus indoor contributions to indoor particulate matter (PM) determined by mass balance methods. J Air Waste Manage Assoc. 2004; 54: 1188-1196.   DOI
12 Sibel M, Munevver A, Abbas, YR, Gulen, G. Bacterial and fungi levels in various indoor and outdoor environments in Ankara, Turkey. Soil Air Water. 2009; 37(6). 487-493.   DOI
13 ACGIH. Bioaerosols Assessment and Control ACGIH, 1999
14 Moschandreas DJ, Pagilla KR, Storino LV. Time and space unifomity of indoor bacteria concentrations in Chicago area residences. Aerosol Sci Tech. 2003; 37(11): 899-906.   DOI
15 Moon HJ, An KA, Choi MS. The status and causes of indoor airborne microorganisms activities in residential buildings. J Korean Soc Living Environ Sys. 2012; 19(6): 669-675.
16 Lee SC, Li WM, Ao CH. Investigation of indoor air quality at residential homes in Hong Kong, Atmos Environ. 2002; 36(2): 225-237.   DOI
17 Lee SH, Jeong SK, Lee KS, Min KW, Kim HS, Kim DS, et al. A study on characteristics of indoor air pollutants using regression analysis in public facillities. J Korean Soc for Environ Anal. 2013; 16(2): 123-132.
18 Lee JH, Jo WK. Characteristics of indoor and outdoor bioaerosols at Korean high-rise apartment buidlings. Environ Res. 2006; 101(1): 11-17.   DOI
19 Lee JH, Jo WK. Characteristics of indoor and outdoor bioaerosols at Korean high-rise apartment buildings. Environ Res. 2006; 101(1): 11-17.   DOI
20 Scheff PA, Paulius VK, Curtis L, Conroy LM. Indoor air quality in a middle school, Part II: development of emission factors for particulate matter and bioaerosols. Appl Occup Environ Hyg. 2000; 15(11): 835-842.   DOI
21 Lou X, Fang Z, Si G. Assessment of culturable airborne bacteria in university campus in Hangzhou. Southeast of China. Afr J Microbio Res. 2012; 6(3): 665-673.
22 Robertson CE, Baumgartner LK, Harris JK, Peterson KL, Stevenss MJ, Frank DN, et al. Cultureindependent analysis of aerosol microbiology in a metropolitan subway system. Appl environ microbiol. 2013; 79(11): 3485-3493.   DOI
23 Kim NY, Kim YR, Kim MK, Cho DW, Kim JS. Isolation and charcterization of airborne bacteria and fungi in indoor environment of elementary schools. Kor J Microbio. 2007; 43(3): 193-200.
24 Asan A, Sen B, S, Sarica. Airborne fungi in urban air of Edirne City (Turkey). Biologia. 2002; 57(1): 59-68.
25 Ekhaise FO, Ighosewe, OU, Ajakpovi OD. Hospital indoor airborne microflora in private and mary card. Infect Control Hosp Epidemiol. 2008; 22: 493-498.
26 Mandal J, Brandl H. Bioaerosols in indoor environment - A review with special reference to residential and occupational locations. Open Environ Biol Monit J. 2011; 4: 83-96.   DOI
27 Kim KY, Cho MS, Kim YS, Lee CM. Study on outdoor distribution characteristics of airborne bacteria in Korea, Korean Soc Atmos Environ. 2009; 5: 115-117.
28 Jeon BH, Hwang IY. Concentrations of total culturable microorganisms and its identification in public facilities, J Korea Acad Industr Coop Soc. 2015; 16(1): 868-876.   DOI
29 Kim JB, Kim JC. Antibioti resistance of food-borne pathogens isolated an indoor environment of a lunchroom in child center. Korean J Environ Health. 2012; 38(5): 415-423.   DOI
30 Bonetta S, Bonetta S, Mosso S, Sampo S, Carraro E. Assessment of microbiological indoor air quality in an Italian office building equipped with an HVAC system. Environ Monit Assess. 2010; 161(1): 473-483.   DOI
31 Leila S, Mohammad R, Mohammad-Zadeh M, Saadat M. Comparison of CHROMagar acinetobacter and conventional methods for isolation Acinetobacter baumanni from tracheal aspirates patients with ventilator-associated pneumonia(VAP). Annals of Biol Res. 2013; 4: 46-52.
32 David W, Marcus, HY, Leung, Lee PKH. Indoor air bacteria communities in Hong Kong households assemble independently of occupant skin microbiomes. Environ Microbiol. 2015; 18(6): 1754-1763.   DOI
33 Aydogdu H, Asan A, Otkun MT. Indoor and outdoor airborne bacteria in child day-care centers in Edirine City(Turkey), seasonal distribution and influence of meteorological factors. Environ Monit Assess. 2010; 164(1): 53-66.   DOI
34 Dutkiewicz J, Krysinska-Traczyk E, Skorska C, Cholewa G, Sitkowska J. Exposure to airborne microorganisms and endotoxin in a potato processing plant. Ann Agri Environ Med. 2002; 9(2): 225-235.
35 Sohn JR, Kim JM, Oh HJ, Nam IS, Yang JH, Lee DH, et al. Assement of particles and bioaerosol concentrations at daycard centers in Seoul. J Odor Indoor environ. 13(1), 40-48.   DOI
36 Mondogin EF, Shapir N, Daugherty SC, DeBoy RT, Emerson JB, Shvartzbeyn, A, et al. Secrets of soil survival revealed by the genome sequence of Arthrobacter aurescens TC1, PLoS genet. 2006; 2(12): 2094-2106.
37 Fang Z, Ouyang Z, Zheng H, Wang X, Hu L. Culturable airborne bacteria in outdoor Environments in Beijing. China. Microb Ecol. 2007; 54(3): 487-496.   DOI