• 동의신경정신과학회지
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• 제19권1호
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• pp.125-146
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• 2008

Objectives : The purpose of this study is to suggest for the following experimental study of dementia by reviewing recent oriental medicine journals that have been published since 2000. Methods: We have investigated various types of studies in relation to dementia through 90 articles that have been published from 2000 to 2007 in recent oriental medicine journals were registered Korea research foundation. Results and Conclusions : 1. Since 2000, 88 articles in relation to dementia have been published and almost of them are herbal medicine-centered studies. Also they show a tendency to increase every year. The journal of oriental neuropsychiatry carries the highest number of studies in relation to dementia. 2. According to the experimental paper, there are 30 cases of using herb simplexes, 48 cases of herb-combined prescription, and 10 cases of other ways. Especially 7 cases of using herb-combined prescription relation to Sasang constitution are all for the Taeumin. 3. There are 85 cases of Animal and cellular experimental, 60 cases of using pathologic model induced cytotoxic activity, a case of using L-NAME, 3 cases of 192 saporin, 4 cases of ibotenic acid, 10 cases of focal cerebral ischemia, 3 cases of alcohol-administered, and one case of natural degradation. 4. Moms water maze, Radial arm maze Passive avoidance learning model were using for examining learning and memory of model animal 5. We propose that following studies of dementia are to he investigated of the applied method of using siRNA with tranceduced gene, sample preparation by water-soaking, oriental medical diagnosis, standardization of differentiating symptom and herb simplexes, building the database by classified prescriptions, and experiment model which are based on precise examining mechanism with cell line as like mouse H19-7 hippocampus, rat HT22 hippocampus, astrocyte, microglia, using the model of animals at APP, PS1, BACE, CT99/PS1, APOE4, Tau, APP/PSI/Tau

• 생약학회지
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• 제49권4호
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• pp.312-321
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• 2018

Recently, consumer demand for functional cosmetics containing natural ingredients has been greatly expanded. To develop the natural cosmetic materials, we selected 3 plants, Chrysanthemum zawadskii Herbich (CZ), Perilla frutescens (L.) Britton var. acuta Kudo (PF), and Rosa multiflora Thunberg (RM) which showed high total flavonoid contents (TFC), total polyphenol contents (TPC), and strong DPPH radical scavenging effect. We determined astragalin, chlorogenic acid, and rosmarinic acid as a marker compound for quantitative analysis of the content of each material and standardization of the quality standards and manufacturing standards through LC/MS analysis. HPLC-DAD was used to simultaneously analyze these marker components of three natural product complexes (Mix) and to validate the analytical method through experiments such as linearity, accuracy and precision. The detection wavelengths were set at 210, 265, and 330 nm. The detected 3 compounds from extract of CZ, PF, RM showed significant linearity ($R^2${\geq_-}$0.9947). The limit of detection (LOD) of chlorogenic acid, astragalin and rosmarinic acid were $8.29{\mu}g/ml$, $2.28{\mu}g/ml$, and $27.00{\mu}g/ml$, respectively. The limit of quantification (LOQ) of chlorogenic acid, astragalin and rosmarinic acid were $25.11{\mu}g/ml$, $6.92{\mu}g/ml$, and $81.83{\mu}g/ml$, respectively. The contents of the three indicators of Mix were 19.82-24.71 mg/g of chlorogenic acid, 43.80-46.02 mg/g of astragalin, and 46.33-48.57 mg/g of rosmarinic acid.

• 한국식품영양학회지
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• 제36권2호
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• pp.93-102
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• 2023

Centella asiatica (C. asiatica) has been widely used in food, cosmetics, and pharmaceutical industry as a functional material. In a previous study, we have investigated not only pharmacological effects such as antioxidative and anti-inflammatory effects, but also analyzed various functional ingredients. In this study, triterpenoids were analyzed using HPLC-DAD to determine marker compounds among functional ingredients. When triterpenoids were analyzed, asiaticoside from C. asiatica was determined as an optimal marker compound. Next, specificity, linearity, limited of detection (LOD), limited of quantification (LOQ), precision, accuracy, and range were evaluated using HPLC-DAD to determine asiaticoside contents in C. asiatica juice and extracts. The specificity was elucidated by chromatogram and retention time using an established analytical method. The coefficient of correlation obtained was 0.9996. LOD was 4.99 ㎍/mL and LOQ was 15.12 ㎍/mL. Intra- and inter-day precision of asiaticoside were determined to be 0.48~1.68% and 0.08~1.09%, respectively. Furthermore, the recovery rate of asiaticoside was 98.88% and the analytical range of Field-70E was determined to be 0.625~10 mg/mL. As a results of evaluating ABTS, DPPH, and FRAP antioxidative effect, Field-70E showed potent antioxidant activities. Results of this study could be used as basic data for quality standardization of C. astiatica juice and extracts.

• 한국축산식품학회지
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• 제43권5호
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• pp.805-825
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• 2023

This experiment aims to investigate the impact of probiotic feed on growth performance, carcass traits, plasma lipid biochemical parameters, intramuscular fat and triglyceride content, fatty acid composition, mRNA expression levels of genes related to lipid metabolism, and the activity of the enzyme in Sunit sheep. In this experiment, 12 of 96 randomly selected Sunit sheep were assigned to receive the basic diet or the basic diet supplemented with probiotics. The results showed that supplementation with probiotics significantly increased the loin eye area, and decreased plasma triglycerides and free fatty acids, increasing the content of intramuscular fat and triglycerides in the muscle and improving the composition of the fatty acids. The inclusion of probiotics in the diet reduced the expression of adenosine 5'-monophosphate-activated protein kinase alpha 2 (AMPKα2) mRNA and carnitine palmitoyltransferase 1B (CPT1B) mRNA, while increasing the expression of acetyl-CoA carboxylase alpha (ACCα) mRNA, sterol regulatory element-binding protein-1c (SREBP-1c) mRNA, fatty acid synthase mRNA, and stearoyl-CoA desaturase 1 mRNA. The results of this study indicate that supplementation with probiotics can regulate fat deposition and improves the composition of fatty acids in Sunit sheep through the signaling pathways AMPK-ACC-CPT1B and AMPK-SREBP-1c. This regulatory mechanism leads to an increase in intramuscular fat content, a restructuring of muscle composition of the fatty acids, and an enhancement of the nutritional value of meat. These findings contribute to a better understanding of the food science of animal resources and provide valuable references for the production of meat of higher nutritional value.

• 한국식품위생안전성학회지
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• 제28권4호
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• pp.330-336
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• 2013

현재 대황은 지표성분의 규격이 한국, 일본과 중국이 다르고, 유통시장에서 대황과 종대황이 혼재되어 있으며 다양한 기원식물이 혼재되어 수입되고 있다. 따라서 대황의 효능을 현대 약리학적으로 해석하는 과정을 통해 약재의 표준화, 과학화 및 위염에 대한 지표성분 검색에 기여할 수 있을 것으로 사료되어 Rheum tanguticum(당고특대황)과 그 비교생약으로 Rumex cripus(양제근), Rheum officinale(약용대황), Rheum palmatum(장엽대황) 및 Rheum undulatum(종대황)의 물 및 70% 에탄올 추출물을 비교하였다. 그 결과, 흰 쥐를 이용한 위염 모델에서 양제근 70% 에탄올 추출물(73.2%)와 종대황 물 추출물(71%)이 각각 70% 이상으로 우수한 위손상 억제활성을 보여주었지만, 당고특대황의 70% 에탄올 추출물 투여시 91.8%로 실험군 중 가장 우수한 위염 억제 효과를 확인할 수 있었다. 공격인자로써의 산을 중화시킬 수 있는 능력을 알아보기 위한 제산력 시험법으로는 당고특대황 물 추출물과 70% 에탄올 추출물이 각각 약 14.2%, 약 15.8%로 억제함을 알 수 있었다. 이 결과로 보아 양성대조군으로 사용한 hydrotalcite(49.9%)보다는 제산작용이 약하였지만 과량 혹은 장기간 복용하면 효과가 있을 것으로 생각된다. 소화성궤양, 위암 및 변연부 B세포 림프종 발생에 있어 중요한 원인 인자로 밝혀졌고, 기능성 소화불량증, 위의 과형성 용종, 철결핍성 빈혈 등 기타 질환과의 연관성들이 제시되고 있는 H. pylori에 대한 colonization 억제 활성을 확인한 결과 당고특대황의 70% 에탄올 추출물 $100{\mu}g/mL$에서 H. pylori에 대한 항균작용(+)을 확인할 수 있었다. DPPH 용액을 이용하여 free radical의 소거능을 $IC_{50}$으로 나타낸 결과, 당고특대황은 물 및 70% 에탄올 추출물에서 $IC_{50}$이 $25.9{\mu}g/mL$, $5.8{\mu}g/mL$로 양성대조군으로 사용한 L-ascorbic acid ($IC_{50}=6.5{\mu}g/mL$)와 유사한 free radical 소거능을 나타내었다. 이 결과로 당고특대황의 70% 에탄올 추출물이 휜 쥐 위염모델의 위손상을 가장 우수하게 억제하였으며 항산화 활성 또한 가장 우수한 것으로 확인되었다. 당고특대황의 성분인 chysophanol, chrysophanol-8-O-glc, desoxyrhaponticin desoxyrhapontigenin, emodin, isorhaponticin, 2-methoxy-4-hydroxyanthraquinone-5-O-glc, physcion, piracetannol-3'-O-glc, resveratrol, rhaponticin 및 rhapontigenin을 이용하여 실험한 결과로는 효성분들 중 physcion $100{\mu}M$과 rhaponticin $50{\mu}M$ 및 $100{\mu}M$에서는 실험에 사용한 성분 들 중 비교적 우수한 colonization 억제작용(+)을 나타내었고, DPPH radical 소거 활성은 isorhaponticin, piracetannol-3'-O-glc, resveratrol 및 rhaponticin에서 $IC_{50}$이 각각 $129.5{\mu}M$, $94.1{\mu}M$ $80.1{\mu}M$ 및 $104.6{\mu}M$로 확인되었다. 실험결과를 토대로 항위염 효과가 있는 우수한 약용식물로써 천연물 의약품개발 가능성을 확인하였고 나아가 한약재의 효능을 과학적으로 검증하고 한약재 표준화에 기여할 수 있을 것으로 기대된다.

• 한국작물학회지
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• 제64권4호
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• pp.452-458
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• 2019

율무 새싹 추출물의 riboflavin과 coixol의 원료표준화를 위한 동시분석 조건을 검증하고, 율무 새싹의 전초와 잎 추출물의 항산화 활성과 함량의 상관관계에 대해 조사하였다. 1. HPLC 분석을 위한 riboflavin과 coixol의 피크가 각각 분리되어 특이성을 확인하였고, 일내 및 일간 정확성은 모두 회수율이 100.28%에서 103.98% 사이이며 정밀성은 모두 1.93%이하를 나타내므로 본 실험의 분석조건은 높은 정확성과 정밀성을 가지고 있다. 2. Riboflavin과 coixol의 동시분석 조건의 검출한계(LOD) 및 정량한계(LOQ)를 조사한 결과 검출한계는 0.71 및 0.31 ㎍/mL, 정량한계는 2.71 및 0.94 ㎍/mL이므로 확립된 HPLC 분석조건은 riboflavin과 coixol의 동시분석하기에 무리가 없는 분석법으로 조사되었다. 3. 율무 새싹의 전초와 잎의 riboflavin과 coixol의 함량을 본 연구에서 얻어진 동시분석법을 적용하여 잎의 환류 추출물에서 가장 높은 함량이 나타났다. 4. 항산화 활성 측정 시, DPPH와 ABTS⁺ 소거능에서 모두 율무 새싹의 전초보다 잎에서, 상온 추출보다 환류 추출이 더 낮은 IC₅₀값을 나타내었다. 율무 새싹 추출물의 riboflavin과 coixol 및 항산화 활성과의 상관관계를 실시한 결과, coixol이 증가할수록 DPPH와 ABTS⁺ 소거능의 IC₅₀값이 유의적으로 감소하는 경향이 나타나므로 원료표준화 지표뿐만 아니라 기능성 지표로 활용이 가능할 것으로 판단되었다.

• 생명과학회지
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• 제20권3호
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• pp.424-429
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• 2010

본 연구에서는 하동 지역에서 제조되고 있는 13종의 매실 당절임 제품을 수집하여 외관, 조직감, 신맛, 단맛 및 전체적인 기호도 등 관능평가 결과 우수한 제품 5종을 선별하여 이화학적 및 제조 특성을 조사하였다. 5종의 매실 당절임 제품의 수분함량은 45.2~53.3%였으며 pH는 약 2.8 내외로 모든 실험구가 유사하게 나타났다. 산도는 0.4% 내외로 모든 당절임 제품에서 유사하였고 당도는 13번 제품이 다른 실험구들에 비해 다소 낮게 나타나 당과 산의 함량이 낮은 제품의 관능적 기호도가 높을 것으로 생각된다. 또한 조단백질, 조지방 함량도 모든 실험구들에서 각각 약 0.4, 0.8% 내외로 유사하게 나타났다. 관능평가시 조직감이 가장 우수하다고 선별된 13번 제품의 기계적 경도 값이 다른 구에 비해 높게 나타났으며 색도는 밝은 색에 황색과 청색에 가까운 제품이 소비자의 관능에 우수할 것으로 생각된다. 5종의 우수한 매실 당절임 제품의 주요 유기산은 수산, 사과산, 구연산 및 푸마르산으로 나타났으며 구연산 함량은 전체 유기산 함량의 60% 이상 수준이었다. 또한 주요 유리당은 glucose, fructose, rhamnose 및 sucrose로 나타났으며 glucose 함량은 전체 유리당 함량에 65% 이상 검출되어 당도 결과와 유사한 경향을 나타내었다. 하동지역에서 제조되고 있는 매실 당절임 제품 13종의 관능검사 결과 기호도가 우수한 5종의 제품 중 2, 10 및 11번은 하동지역에서 재배되는 품종을 알 수 없는 재래종이었고 12번 제품은 남고(南高), 13번 제품은 백가하(百加賀) 품종으로 제조된 것이었다. 또한 매실 과육과 자당의 배합비율은 5.5 : 4.5로 제조된 13번 제품을 제외하고 4종의 제품에서 동일한 비율로 제조하였으며 7개월 이상 저온에서 숙성시킨 것으로 조사되었다. 따라서 하동지역에서 주로 생산되고 지역적 상품화가 될 수 있는 매실 당절임을 제조하기 위해서는 백가하 품종으로 과육과 자당을 5.5 : 4.5 비율로 배합하여 $5^{\circ}C$의 저온에서 약 7개월 이상 숙성시켜 제조하는 것이 가장 적합 할 것으로 생각된다.

• 동서간호학연구지
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• 제10권1호
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• pp.11-26
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• 2004

The purpose of this study is to investigate the present conditions of nursing investment contents, its conversion process, and output in Oriental University Medical Center, Korea to get good qualified Oriental nursing result which is the ultimate purpose of the Oriental nursing management, and to develope a matrix of Oriental nursing management system on the basis of that project. The subjects for nursing investment and output contents were eighteen nursing directors in eleven Oriental University Medical Center and two hundred thirty-nine nurses with three years and over experience in Oriental medical center. The subjects for Oriental nursing organization, human affair management, and control function were nineteen Oriental medical center in Oriental University Medical Center, Korea. Data were collected from November, 2002 to February, 2003 with questionnaire. Data analysis was done by SPSS PC+ 12 program. Frequency, percentage, and minimum/maximum values were used for investment contents, and frequency and percentage were used for conversion process and output contents. 1. The input factors of oriental nursing management system The objective's western hospital career was over five years of one hundred and seventy-five(73.2%) persons. Nursing in-service education was performed in fourteen hospitals(77.8%). Two hundreds(83.7%) were pro to oriental nurse system. Only four hospitals(22.2%) had independent budget in nursing division. Nursing staff allocation to the bed was from 2.8:1 to 9.06:1 respectively, with a big gap of the rate following the hospitals. 2. The conversion factors of oriental nursing system 1) Oriental nursing system Oriental hospital nursing system was organized independently in ten hospitals among eighteen hospitals. The recruitment of nurses which was a vital role of the nursing division of the hospital was mostly(79%) opened. The education to develope nursing personnels was through in-service one in 97.4%. Education for oriental nursing and management was performed in 42.1%(eight hospitals) and that for reserves was done in 36.8%(seven hospitals). Administration for nursing education by nursing division was 68.5%(thirteen hospitals). The post education evaluation was performed by report submission in 36.8%(seven hospitals), by written examination in 26.3%, by questionnaires in 21.1%, and by lecture presentation in 15.8% subsequently. The directorial meeting for the nursing directors was attended by 84.2%(sixteen hospitals), and the meeting type was the medical executive and support division executive meeting in 55.6%(ten hospitals) and the personnel management in 39.6%(seven hospitals). 2) The actual conditions of oriental nursing personnel management The reason of working in oriental hospital was by voluntary in 67.1%(a hundred and sixty persons), by nursing department order in 28.0%(sixty-seven persons), and by others in 5.0%(twelve persons) respectively. The shift form was a three-shifts one in 94.7%(eighteen hospitals), a two-shift one in only one hospital. Duty assignment was functional in 52.6%(ten hospitals), team and functional in 26.3%(five hospitals) and no team alone. Promotion manual was present at 68.4%(thirteen hospitals) and the competency essentials comprised of performance evaluation in 79%, interview, written examination, training result, study result subsequently. No labor union existed in 79%(fifteen hospitals) 3) Oriental nursing preceptor system There were five oriental hospitals(27.7%) administering the preceptor utilization model, which showed lower rate than the twenty-two medical university hospitals in Seoul in which fifteen hospitals (72.7%) were having the system. To the question of necessity of oriental nurse system asked to the objectives of two hundred and thirty-nine with more than three year-experience in oriental hospital, two hundred persons(83.7%) answered positively. 4) The control of oriental nursing The evaluation results from the target hospitals were mostly not opened in 89.4% of oriental hospitals. Thirteen hospitals(68.3%) had evaluation system of direct managers and the next were three hospitals(15.8%) of direct managers and selves. There was one hospital(5.3% each) where fellows and superiors, fellows, and inferiors' evaluation was performed and no hospital where superiors, fellows, inferiors and selves, and superiors, fellows and selves' evaluation was performed. The QI activity of nursing was 42.1%(eight hospitals) for nursing service evaluation, 36.8% for survey of ECSI, 26.3% for survey of ICSI, 15.8% for medical visit rate, 10% for hospital standardization inspection in sequence. 3. The output factors of oriental nursing management system The job satisfaction appeared good in general, indicating very good in thirty-seven persons (15.7%), good in one hundred and fourteen persons (48.3%) and fair in eighty-five persons(36.0%).

• 한국식품영양학회지
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• 제36권6호
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• pp.551-560
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• 2023

This study was performed to investigate antioxidant and anti-inflammatory activities of perilla(Perilla frutescens L.) seed, flower and leaf according to extraction condition. Perilla seed extracts(PSE), perilla flower extracts(PFE), perilla leaf extracts(PLE) was extracted by stirring extraction (STE, 25℃), shaking extraction (SHE, 80℃), and sonication assisted extraction(SAE, 25℃) with 94% ethanol, 60% ethanol and distilled water, followed by analysis of total polyphenol and flavonoid and testing radical scavenging activities. The highest total polyphenol content (5.47, 9.36, 38.58 mg gallic acid equivalent/g), total flavonoid content(5.77, 8.62, 46.44 mg catechin equivalent/g), ABTS(10.68, 19.46, 63.56 mg trolox equivalent/g) and DPPH(6.51, 7.69, 79.73 mg trolox equivalent/g) radical scavenging activity of PSE, PFE and PLE was observed in the HWE with 60% ethanol,. Among the three extraction method, SHE provided the best results for yield, polyphenol, flavonoid content of perilla seed, flower, leaf in comparison to STE or SAE. SHE with 60% ethanol of perilla seed, flower, leaf more effectively inhibited secretion of nitric oxide(NO) and pro-inflammatory cytokine in RAW 264.7 macrophage exposed to LPS compared to other extraction solvent and method. Therefore, these extracts obtained from perilla seed, flower, leaf could be used antioxidant and anti-inflammatory ingredients in the food industry.