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Vision Based Non Contact Elongation Measurement in Universal Testing Machine [UTM] (만능물성시험기[UTM]에 있어서 새로운 영상기반의 비접촉식 신룰측정방법)

  • No, Jae-Myeong;Park, Hye-Won;Kim, Ho-Cheol;Kim, Yong-Dae;Lee, Wang-Heon;Park, Yong-Su
    • Proceedings of the KIEE Conference
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    • 2008.10b
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    • pp.298-299
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    • 2008
  • The materials are measured and analyzed by the UTM combined with a contact type extensometer so as to analyze the characteristics such as strain-stress curve. However, the JIG and Fixture utilized in the UTM according to the standard [ASTM] can not only scratch the specimens but also have a serious distort on test result by the weight of the ZIG itself. In this paper we propose a moncular vision based visual extensometer [VE] securing the measuring accuracy using a new cross correlation in detecting the two feature points previously marked on the specimen from two successive images, and verify the usefulness of this VE through a real experiment on the UTM.

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Detection of Fusarium Species by Enzyme-Linked Immunosorbent Assay Using Monoclonal Antibody

  • Kwak, Bo-Yeon;Kwon, Byung-Joon;Kweon, Chang-Hee;Shon, Dong-Hwa
    • Journal of Microbiology and Biotechnology
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    • v.13 no.5
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    • pp.794-799
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    • 2003
  • Enzyme-linked immunosorbent assay (ELISA) was developed for the rapid detection of Fusarium species, known as harmful fungi in food. One of the hybridoma cell lines (lB8) which produced a monoclonal antibody (Mab) specific to Fusarium extracellular polysaccharide (EPS) was screened and the Mab was produced and purified. A detection limit of the sandwich ELISA against F. moniliforme EPS was $0.001\;\mu\textrm{g}/ml$ in the standard curve. Among the 59 strains tested, most Fusarium species showed hight reactivity with Mab lB8, even when the culture broths were diluted 100,000 times. On the other hand, the other genera, except A. versicolor and Trichoderma viride, had no reactivity. When 1 to $100\;\mu\textrm{g}$ of F. moniliforme EPS was spiked into rice, potato, and mandarine orange, the average recoveries were 151%, 84%, and 94%, respectively, determined by sandwich ELISA. The correlation coefficients between the EPS levels determined by sandwich ELISA and the dry mycelial weight of the liquid culture of F. moniliforme, as well as between the EPS and colony forming unit in solid culture of potato, were 0.97 and 0.91, respectively.

A Study on Characteristics of Damageability and Repairability with Similar Platform Type at Low Speed 40% Offset Crash Test (동일 플렛폼 차량에 대한 저속 충돌시 손상성 수리성에 미치는 영향에 관한 연구)

  • Lim, Jong-Hun;Park, In-Song;Heo, Seung-Jin
    • Transactions of the Korean Society of Automotive Engineers
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    • v.13 no.2
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    • pp.108-113
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    • 2005
  • The damageability and repairability of similar platform type vehicles could be very concerned with design optimization. In all the vehicles crash tested, small size passenger vehicles were weakness in aspect of damageability and repairability. The most critical area appears to be repair cost considering that parts cost is the largest portion of total repair cost segments. Besides repair cost, attaching method of front sidemember and subframe are placed special importance for impact energy absorption and damageability and repairability. So in order to improve damageability and repairability of vehicle structure and body component of the monocoque type passenger vehicles, the end of front side member and front back beam should be designed with optimum level and to supply the end of front side member as a partial condition approx 300mm. The effectiveness of design concept on the 40% offset frontal impact characteristics of the passenger vehicle structure is investigated and summarized.

Pullout Performance of Reinforcing Fiber Embedded in Nano Materials Cement Mortar with Nano Clay Contents (나노클레이 첨가량에 따른 나노재료 시멘트 모르타르에 정착된 보강섬유의 인발성능)

  • Oh, Ri-On;Park, Chan Gi
    • Journal of The Korean Society of Agricultural Engineers
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    • v.55 no.3
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    • pp.113-121
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    • 2013
  • Recently, it has been studied for the application of nano-materials in the concrete. Applied a small amount of nano-materials can achieve the goal of high strength, high performance and high durability. The small addition of nano clay improves strength, thermal stability, and durability of concrete because of the excellent dispersion. The present study has investigated the effectiveness, when varying with the contents of nano clay, influencing the pull-out behavior of macro synthetic fibers in nano materials cement mortar. Pullout tests conducted in accordance with the Japan Concrete Institute (JCI) SF-8 standard for fiber-reinforced concrete test methods were used to evaluate the pullout performance of the different nano clay. Nano clay was added to the 0, 1, 2, 3, 4 and 5 % of cement weight. The experimental results demonstrated that the addition of nano clay led to improve the pull-out properties as of the load-displacement curve in the precracked and debonded zone. Also, the compressive strength, flexural strength and pullout performance and of Mix No. 1 and No. 2 increased up to the point when nano clay used increased by 2 and 3 % contents, respectively, but decreased when the exceeded 3 and 4 %, respectively. It was proved by verifying increase of the scratching phenomenon in macro synthetic fiber surface through the microstructure analysis on the surface of macro synthetic fiber.

Cooking Properties of Dry Noodles Prepared from HRW-WW and HRW-ASW Wheat Flour Blends (미국밀과 호주밀의 제면성 비교)

  • Shin, Sung-Young;Kim, Sung-Kon
    • Korean Journal of Food Science and Technology
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    • v.25 no.3
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    • pp.232-237
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    • 1993
  • The rheological properties of hard red winter, western white and Australian standard white wheat flours and of HRW-WW and HRW-ASW wheat flour blends having the same protein content were studied. Cooking properties of dry noodles prepared from HRW-WW and HRW-ASW wheat flour blends were also investigated. The noodles were prepared with salt and alkaline reagent. The salt and alkaline concentrations used were 1.7% and 0.17%. respectively, based on the weight of wheat flour. The alkaline reagent was an equal mixture of sodium carbonate and potassium carbonate. The HRW-ASW wheat flour blend had higher farinograph absorption and slightly stronger curve than HRW-WW wheat flour blend. Salt decreased the absorption of wheat flours by 2 and of wheat flour blends by 1%. However, alkaline reagent essentially had no effort on farinograph absorption. Salt and alkaline reagent strengthened the dough of wheat floors and wheat flour blends, with the former being more effective. No significant differences in pasting properties between HRW-WW and HRW-ASW wheat flour blends by amylograph were observed. There were no significant differences in rate of increase of weight or volume between noodles prepared from HRW-WW and HRW-ASW wheat flour blends. Alkaline reagent did not affect the weight gain of noodles hut lowered the volume gain. Breaking force of dry noodles and cutting force of cooked noodles were similar between the two noodles. Alkaline reagent increased both the breaking and cutting forces of noodles. Sensory evaluation revealed that the noodles prepared from HRW-WW and HRW-ASW wheat flour blends were slightly different. but not different from each other by preference test.

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Fast Video Data Encryption for Swarm UAVs Using Hybrid Crypto-system (하이브리드 암호시스템을 이용한 군집 영상의 고속 암호화)

  • Cho, Seong-Won;Kim, Jun-Hyeong;Chae, Yeo-Gyeong;Joung, Yu-Min;Park, Tae-Kyou
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.46 no.7
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    • pp.602-609
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    • 2018
  • This paper proposes the hybrid crypto-system for fast video data encryption of UAV(Unmanned Aerial Vehicle) under the LTE(Long-Term Evolution) wireless communication environment. This hybrid crypto-system is consisted of ECC(Elliptic Curve Cryptography) public key algorithm and LEA(Light-weight Encryption Algorithm) symmetric key algorithm. ECC is a faster public key algorithm with the same security strength than RSA(Rivest Shamir Adleman), and Korean standard LEA with the same key size is also a faster symmetric key algorithm than AES(Advances Encryption Standard). We have implemented this hybrid crypto-system using OpenSSL, OpenCV and Socket programs under the Swarm 8-UAV. We have shown the efficient adaptability of this hybrid crypto-system for the real-time swarm UAV through the experiments under the LTE communication environment.

Hearing loss screening tool (COBRA score) for newborns in primary care setting

  • Poonual, Watcharapol;Navacharoen, Niramon;Kangsanarak, Jaran;Namwongprom, Sirianong;Saokaew, Surasak
    • Clinical and Experimental Pediatrics
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    • v.60 no.11
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    • pp.353-358
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    • 2017
  • Purpose: To develop and evaluate a simple screening tool to assess hearing loss in newborns. A derived score was compared with the standard clinical practice tool. Methods: This cohort study was designed to screen the hearing of newborns using transiently evoked otoacoustic emission and auditory brain stem response, and to determine the risk factors associated with hearing loss of newborns in 3 tertiary hospitals in Northern Thailand. Data were prospectively collected from November 1, 2010 to May 31, 2012. To develop the risk score, clinical-risk indicators were measured by Poisson risk regression. The regression coefficients were transformed into item scores dividing each regression-coefficient with the smallest coefficient in the model, rounding the number to its nearest integer, and adding up to a total score. Results: Five clinical risk factors (Craniofacial anomaly, Ototoxicity, Birth weight, family history [Relative] of congenital sensorineural hearing loss, and Apgar score) were included in our COBRA score. The screening tool detected, by area under the receiver operating characteristic curve, more than 80% of existing hearing loss. The positive-likelihood ratio of hearing loss in patients with scores of 4, 6, and 8 were 25.21 (95% confidence interval [CI], 14.69-43.26), 58.52 (95% CI, 36.26-94.44), and 51.56 (95% CI, 33.74-78.82), respectively. This result was similar to the standard tool (The Joint Committee on Infant Hearing) of 26.72 (95% CI, 20.59-34.66). Conclusion: A simple screening tool of five predictors provides good prediction indices for newborn hearing loss, which may motivate parents to bring children for further appropriate testing and investigations.

Quantitative Analysis and Qualification of Amitrole Using LC/ESI-MS (LC/ESI-MS를 이용한 아미트롤의 정성확인 및 정량분석)

  • Park, Chan-Koo;Eo, Soo-Mi;Kim, Min-Young;Sohn, Jong-Ryeul;Mo, Sae-Young
    • Analytical Science and Technology
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    • v.17 no.2
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    • pp.117-129
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    • 2004
  • Amitrole in environment, difficult to be analyzed by GC or GC/MS due to high polarity and low volatility, was analyzed by LC/ESI/MS in the study. Maximum peak intensity of amitrole in LC/MS/ESI mass spectrum is m/z 85 of protonated molecular ion $(M+H)^+$ with 30V of cone voltage at SIR mode. It was confirmed that ratios between main ion of amitrole, 85 of protonated molecular ion, and m/z 58 fragmented ion of amitrole, had increased corresponding with the increment of cone voltage from 20V to 70V. The isotope molecular weight of amitrole was $86([M+H])^+$ at LC/MS analysis and the mass spectrum ratio between 85 mass and 86 mass was not different by the change of concentration but similar to theoretical ratio(less than 10% standard deviation).The linearity of standard calibration curve under same condition with sample treatment method had $y=1.09354e^6X+26947.2$ and $r^2=0.99$. Recovery rates in water and soil samples were 77.64~83.44% and 71.11~79.44%, respectively. Reliability of the analysis was performed with 5 repeated measurements at each level of standard concentration and the result showed that relative standard deviation was less than 10%; therefore, the extraction and analysis method in the study suggested that it would be reliable to measure amitrole in water and soil media.

Sasa borealis leaves extract improves insulin resistance by modulating inflammatory cytokine secretion in high fat diet-induced obese C57/BL6J mice

  • Yang, Jung-Hwa;Lim, Hyeon-Sook;Heo, Young-Ran
    • Nutrition Research and Practice
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    • v.4 no.2
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    • pp.99-105
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    • 2010
  • Obesity is considered a mild inflammatory state, and the secretion of inflammation-related cytokines rises as adipose tissue expands. Inflammatory cytokines, including tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), interlukin 6 (IL-6) and monocyte-chemoattractant protein 1 (MCP-1), are modulated by adipose tissue and known to play an important role in insulin resistance which is the common characteristics of obesity related disorders. In this study we analyzed the effects of Sasa borealis leaves extract on inflammatory cytokines and insulin resistance in diet induced obese C57/BL6J mice. The obese state was induced by a high fat diet for 20 weeks and then the mice were divided into two groups; obese control group (OBC, n = 7) and experimental group (OB-SBE, n = 7). The OBC group was fed a high fat diet and the OB-SBE group was fed a high fat diet containing 5% Sasa borealis leaves extract (SBE) for 12 weeks. We also used mice fed a standard diet as a normal control (NC, n = 7). The body weight and adipose tissue weight in the OB group were significantly higher than those in the NC group. The effects of the high fat diet were reduced by SBE treatments, and the body weight and adipose tissue deposition in the OB-SBE group were significantly decreased compared to the OBC group. The OBC group showed higher serum glucose and insulin levels which resulted in a significant increase of incremental area under the curve (IAUC) and HOMA-IR than the NC group. Also, serum leptin, TNF-${\alpha}$, and IL-6 levels were significantly higher in the OBC group than in the NC group. In contrast, the OB-SBE group showed a reversal in the metabolic defects, including a decrease in glucose, insulin, IAUC, HOMA-IR, TNF-${\alpha}$, IL-6 and leptin levels. These results suggest that BSE can suppress increased weight gain and/or fat deposition induced by a high fat diet and theses effects are accompanied by modulation of the inflammatory cytokines, TNF-${\alpha}$ and IL-6 secretion resulting in improved insulin resistance.

Age-related Changes in Plasma Leptin from Early Growing to Late Finishing Stages of Castrated Holstein Steers: Utilizing Multi-species Leptin RIA

  • Vega, R.A.;Lee, H.G.;Kuwayama, H.;Matsunaga, N.;Hidari, H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.5
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    • pp.725-731
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    • 2002
  • This experiment was performed to understand the changes in plasma leptin in association with plasma IGF-1, body weight and ADG from early growing to late finishing stages of Holstein steers. Blood collection was performed by arterial vein puncture at selected monthly ages of 1 (54 kg), 2.6 (103 kg), 7.2 (205 kg), 13.5 (314 kg), 16.9 (414 kg), 22.2 (550 kg), 24.9 (626 kg) and 27.4 months (695 kg). The blood was analyzed for leptin using the multi-species leptin RIA with recombinant bovine leptin (rbleptin) as standard, plasma IGF-1 was also measured using RIA. Against the standard rbleptin, the multi-species Leptin RIA system's sensitivity, cross reactivity, slope and recovery of 41.0 ng/ml rbleptin in plasma were 4.9 ng/ml, 11.22%, -1.396 and 97.8%, respectively. Plasma leptin measured were more than 5.0 ng/ml, which enable multi-species RIA system to investigate plasma leptin in normal growing steers. Body weight resulted to a highly significant second-degree polynomial relationship with plasma leptin (q=0.54, p<0.0001) and plasma IGF-1 (q=0.44, p<0.0001) from 1 to 27.4 monthly ages. However, the second-degree polynomial curve of plasma leptin and IGF-1 differs showing a concave and convex curvilinear relationship, respectively. ADG was not significantly associated to plasma leptin (r=0.06, p>0.05) and plasma IGF=1 (r=0.06, p>0.05) from 1 to 27.4 monthly ages. Low coefficient, but significant associated increase of plasma leptin and IGF-1 (r=0.12, p<0.008) from 1 to 27.4 months was observed. The uncoordinated increases of plasma IGF-1 at growing and plasma leptin at fattening period, may indicate (1) indirect involvement of endogenous IGF-1 on leptin secretion, and (2) IGF-1 level may signify lean and bone accretion while plasma leptin may mirror body fatness across the monthly ages of Holstein steers.