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Quantitative Analysis and Qualification of Amitrole Using LC/ESI-MS  

Park, Chan-Koo (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Eo, Soo-Mi (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Kim, Min-Young (Seoul Metropolitan Government Research Institute of Public Health and Environment)
Sohn, Jong-Ryeul (Department of Environmental Sanitation, College of Health Science, Korea University)
Mo, Sae-Young (Department of Environmental Engineering, Chungbuk University)
Publication Information
Analytical Science and Technology / v.17, no.2, 2004 , pp. 117-129 More about this Journal
Abstract
Amitrole in environment, difficult to be analyzed by GC or GC/MS due to high polarity and low volatility, was analyzed by LC/ESI/MS in the study. Maximum peak intensity of amitrole in LC/MS/ESI mass spectrum is m/z 85 of protonated molecular ion $(M+H)^+$ with 30V of cone voltage at SIR mode. It was confirmed that ratios between main ion of amitrole, 85 of protonated molecular ion, and m/z 58 fragmented ion of amitrole, had increased corresponding with the increment of cone voltage from 20V to 70V. The isotope molecular weight of amitrole was $86([M+H])^+$ at LC/MS analysis and the mass spectrum ratio between 85 mass and 86 mass was not different by the change of concentration but similar to theoretical ratio(less than 10% standard deviation).The linearity of standard calibration curve under same condition with sample treatment method had $y=1.09354e^6X+26947.2$ and $r^2=0.99$. Recovery rates in water and soil samples were 77.64~83.44% and 71.11~79.44%, respectively. Reliability of the analysis was performed with 5 repeated measurements at each level of standard concentration and the result showed that relative standard deviation was less than 10%; therefore, the extraction and analysis method in the study suggested that it would be reliable to measure amitrole in water and soil media.
Keywords
Amitrole; EDCs; LC/ESI/MS; Quantification; Qualification;
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