• Asian Pacific Journal of Cancer Prevention
• /
• 제15권21호
• /
• pp.9153-9157
• /
• 2014

Background: Capparis spinosa L., a Uygur medicine, had been shown to have anti-tumor activity in our early experiments with an N-butanol extract (CSBE) as its active fraction. However, the mechanisms responsible for its effects are not clearly understood. Here, we report that treatment of SGC-7901 cells with CSBE resulted in dose-dependent reduction of cell viability and induction of apoptosis. Materials and Methods: To observe the inhibitory and killing effects of CSBE on SGC-7901, the SRB method was adopted, apoptosis being observed by electron microscopy. To clarify the mechanisms of apoptosis, Western blot and enzyme-labeled methods were used to examine the release of cytochrome c (Cyt c) and the activation of the caspase cascade. Results: By electron microscopy, apoptotic morphologic changes were detectable after CSBE administration. In this study, it was also demonstrated that CSBE induced apoptosis in SGC-7901 cells by inhibiting mPTP open, mitochondrial cytochrome c release, caspase-9 and caspase-3 activation. Conclusions: The findings indicated that CSBE induces aap optosis through mitochondrial pathway.

• Advances in Traditional Medicine
• /
• 제5권1호
• /
• pp.29-36
• /
• 2005

Polysaccharide hydrogels constitute a structurally diverse class of biological macromolecules with a wide range of physicochemical properties. They also constitute important members of the family of industrial water-soluble polymers. They find application in Pharmacy as binders, disintegrants, suspending, emulsifying and sustaining agents. According to the International Pharmaceutical Excipients Council (IPEC), an excipient must have an established safety profile. Hence, in the present study, in vitro cytotoxicity on Vero and HEp-2 cell lines, and in vivo acute toxicity in rats were carried out to establish the safety of polysaccharide hydrogels from the seeds of Plantago ovata and Ocimum basilicum. The in vitro cytotoxicity was determined by MTT and SRB assays. In the in vivo acute toxicity, the effects of three different doses of hydrogels (100, 200 and 400 mg/kg body weight) on food and water intake, body weight, biochemical and hematological parameters were studied. The results of in vitro did not show any cytotoxicity on both the cell lines used. In the in vivo acute toxicity, the hydrogels did not show any toxic symptoms in all three dose levels. This establishes the safety of the selected hydrogels. Hence, they can be used as excipients in pharmaceutical dosage forms.

• 한국전자통신학회논문지
• /
• 제5권6호
• /
• pp.588-594
• /
• 2010

다중-홉 센서 네트워크에서 방송은 경로 탐색과 주소 설정 등 많은 응용 작업들을 지원해 주는 기본적인 동작이다. 플러딩을 이용한 방송은 네트워크상에서 방송 폭풍 문제라고 언급되는 중복성, 경쟁, 충돌문제 등을 야기한다. 무선 센서 네트워크를 위한 여러 가지 방송 기법들은 단순 플러딩 기법 보다 우수한 성능을 얻기 위해 제안되었다. 무선 네트워크에서 제안된 방송알고리즘은 플러딩 기법, 확률적 기법, 카운터 기반 기법, 거리 기반 기법, 위치 기반 기법, 그리고 이웃 지식 기반 기법으로 분류 된다. 본 논문에서는 단순 플러딩 기법, 확률적 기법, 카운터 기반 기법, 거리 기반 기법과 이웃 지식 기반 기법을 소개하고, 각 기법들의 성능과 효율성을 네트워크 시뮬레이션을 통해 비교 분석한다.

• Advances in Traditional Medicine
• /
• 제2권1호
• /
• pp.52-57
• /
• 2002

Geranyl phenyl ethers 1, 2, 3, 4 and 5, 5-fluorouracil 6 and adriamycin 7 as references were tested for their growth inhibitory effects against tumor cell lines and NIH 3T3 fibroblasts using two different assays, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) and sulforhodamine B protein (SRB) assays. These results suggest that methyl-4-[{(2E)-3,7-dimethyl-2,6-octadienyl}oxy]-3-methoxy benzoate 1 showed growth inhibition activity against tumor cell lines. The maximum activity exhibited by methyl-4-[{(2E)-3,7-dimethyl-2,6-octadienyl}oxy]-3-hydroxybenzoate 3 against Staphylococcus epidermidis (MIC, $1,000{\mu}g/ml$).

• Asian Pacific Journal of Cancer Prevention
• /
• 제16권15호
• /
• pp.6423-6428
• /
• 2015

The present study was designed to evaluate in vitro anti-proliferative potential of extracts from four Indian medicinal plants, namely Anogeissus latifolia, Terminalia bellerica, Acacia catechu and Moringa oleiferna. Their cytotoxicity was tested in nine human cancer cell lines, including cancers of lung (A549), prostate (PC-3), breast (T47D and MCF-7), colon (HCT-16 and Colo-205) and leukemia (THP-1, HL-60 and K562) by using SRB and MTT assays. The findings showed that the selected plant extracts inhibited the cell proliferation of nine human cancer cell lines in a concentration dependent manner. The extracts inhibited cell viability of leukemia HL-60 and K562 cells by blocking G0/G1 phase of the cell cycle. Interestingly, A. catechu extract at $100{\mu}g/mL$ induced G2/M arrest in K562 cells. DNA fragmentation analysis displayed the appearance of a smear pattern of cell necrosis upon agarose gel electrophoresis after incubation of HL-60 cells with these extracts for 24h.

• 전기전자학회논문지
• /
• 제10권2호통권19호
• /
• pp.109-115
• /
• 2006

본 논문에서는 FCC 규격을 만족하는 DS-UWB 통신을 위한 고차미분 가우시안 펄스 생성기를 제안하고, 이를 실험적으로 구현하였다. 100Mbps의 구형파를 가우시안 펄스로 변환하기 위해 두개의 SRB만을 이용한 가우시안 펄스 발생기를 제작하였다. 출력된 펄스는 330 psec의 펄스폭과 920 mV의 크기를 가진다. 또한 WLAN 대역을 제거하기 위해 마이크로스트립 라인과 병렬 스터브를 이용한 고차미분의 특성을 가지는 이중 대역통과 필터를 설계 및 제작하였다. 최종적으로 WLAN 대역을 -25dB 이상 억제를 하고, 시간축 상에서 1 nsec의 펄스폭과 25 mV의 크기를 가지는 13차 가우시안 펄스를 출력하였다.

• Advances in Traditional Medicine
• /
• 제3권4호
• /
• pp.187-190
• /
• 2003

Seven essential oils were tested for in vitro cytotoxicity against the cancerous cell lines A-549, HEp-2 and DLA and normal BRL-3A, NRK-49F and Vero cell lines using standard MTT, SRB and dye exclusion techniques. The A-549 cell line was found to be the most susceptible to all the essential oils. The essential oils of A. nilagirica, A. calamus and O. sanctum were found to be the more active against these cells with mean $CTC_{50}$ values of 17.75, 19.00 and $24.37\;{\mu}g/ml$, respectively. The essential oil of Acorus calamus was found to be the most potent with low $CTC_{50}$ values against the cancerous and comparatively higher $CTC_{50}$ values against the normal cell lines. Artemisia pellens and Pelargonium graveolens oils also showed potent activity. These oils merit further investigation to identify the active principles and nature of the anti tumor activity in animal models.

• 한국지구물리탐사학회:학술대회논문집
• /
• 한국지구물리탐사학회 2003년도 Proceedings of the international symposium on the fusion technology
• /
• pp.504-510
• /
• 2003

This study has been carried out to evaluate the passive treatment systems for acid mine drainage in Korea and to suggest, if possible, the method for the improvement. 35 passive treatment systems in 27 mines have been constructed since 1996. SAPS, being the main process, was combined with more than one of processes such as anaerobic wetland, aerobic wetland, and oxidation pond for the construction of passive treatment system. Problems observed during the operation include the poor sulfate removal ratio, overflow, leakage, unusabless of the whole system, and inefficiency. The reasons of the poor sulfate removal ratio are believed that the low temperature during the winter prohibits the SRB activity and HRT for bacterial sulfate reduction is insufficient. An alternative method In Adit Sulfate Reducing System which enables to keep the temperature constant at about $15^{\circ}C$ was suggested. IASRS is the methods of placing the SAPS inside the adit, which enables the temperature around the system constant can be maintained. The experiments using the laboratory scaled model systems made up of four sections showed high efficiencies in pH control and metal removal ratios, but showed still low sulfate removal ratio of about $23\%$ also with high COD at the beginning of the operation.

• 한국생산제조학회지
• /
• 제20권6호
• /
• pp.830-836
• /
• 2011

Most of pitch/yaw reducers consist of several planetary geartrains. Planetary geartrains make gearboxes to be small and light, low noise and good efficiency. Most important thing in the planetary geartrain is load distribution on the gear tooth flank. In this study, the effect of output shaft bearings on the load distribution of gear tooth flank has been investigated. The commercial software was employed to compare the load distribution of two models depending on the bearing type. The spherical roller bearing(SRB) and the cylindrical roller bearing(CRB) were used as output shaft bearings in the $1^{st}$ model, and two taper roller bearings(TRB) were used in the $2^{nd}$ model. As a result, it was found that the $2^{nd}$ model. showed better performances on the load distribution of gear tooth flank, this results stated that the output shaft bearing system could be important consideration when designing reducers for wind turbine systems.

• 한국식품영양과학회지
• /
• 제32권7호
• /
• pp.1088-1094
• /
• 2003

차가버섯 에탄올 추출물과 에틸아세테이트 및 물 분획물 그리고 수용성 다당과 불용성 다당 I, II에 대하여 항돌연변이원성 실험과 암세포성장억제 실험을 행한 결과, 각 시료에서 돌연변이원성은 없는 것으로 나타났으며 직접변이원인 MNNG와 4NQO, 간접변이원인 B[a]P와 Trp-P-1 모두에서 시료농도 50$\mu\textrm{g}$/plate에서 불용성 다당 II가 90% 이상의 높은 억제효과를 나타내었다. 또한 암세포 성장 억제 실험에서는 에틸아세테이드 분획물에서 다른 분획이나 시료들보다 시료농도 4 mg/mL에서 A549, AGS, 그리고 MCF-7극 세포에 대하여 각각 90.8%, 94.3%, 83.5%의 높은 암세포 성장억제 효과를 나타내었다.