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http://dx.doi.org/10.7314/APJCP.2014.15.21.9153

N-Butanol Extract of Capparis spinosa L. Induces Apoptosis Primarily Through a Mitochondrial Pathway Involving mPTP Open, Cytochrome C Release and Caspase Activation  

Ji, Yu-Bin (Center of Research and Development on Life Sciences and Environmental Sciences, Harbin University of Commerce)
Yu, Lei (Center of Research and Development on Life Sciences and Environmental Sciences, Harbin University of Commerce)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.21, 2014 , pp. 9153-9157 More about this Journal
Abstract
Background: Capparis spinosa L., a Uygur medicine, had been shown to have anti-tumor activity in our early experiments with an N-butanol extract (CSBE) as its active fraction. However, the mechanisms responsible for its effects are not clearly understood. Here, we report that treatment of SGC-7901 cells with CSBE resulted in dose-dependent reduction of cell viability and induction of apoptosis. Materials and Methods: To observe the inhibitory and killing effects of CSBE on SGC-7901, the SRB method was adopted, apoptosis being observed by electron microscopy. To clarify the mechanisms of apoptosis, Western blot and enzyme-labeled methods were used to examine the release of cytochrome c (Cyt c) and the activation of the caspase cascade. Results: By electron microscopy, apoptotic morphologic changes were detectable after CSBE administration. In this study, it was also demonstrated that CSBE induced apoptosis in SGC-7901 cells by inhibiting mPTP open, mitochondrial cytochrome c release, caspase-9 and caspase-3 activation. Conclusions: The findings indicated that CSBE induces aap optosis through mitochondrial pathway.
Keywords
Capparis spinosa L.; n-butanol extract; mitochondrial pathway; apoptosis;
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