Browse > Article
http://dx.doi.org/10.3746/jkfn.2003.32.7.1088

Antimutagenic and Cytotoxic Effects of Ethanol Extract from the Inonotus obliquus  

함승시 (강원대학교 식품생명공학부)
오상화 (강원대학교 식품생명공학부)
김영균 (국민대학교 임산공학과)
신광순 (경기대학교 식품생명공학과)
장현유 (한국국립농업대학 버섯학과)
정국훈 (두산그룹 생명공학연구조합)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.32, no.7, 2003 , pp. 1088-1094 More about this Journal
Abstract
This study was performed to determine the antimutagenic and cytotoxic effects of ethanol extract, ethylacetate fraction, water fraction and water soluble and insoluble polysaccharides I and II isolated from Inonotus obliquus using Ames test and SRB assay. Each sample itself did not show mutagenic effect. Among samples, the water unsoluble polysaccharides II in the presence of 50 $\mu\textrm{g}$/plate showed the strongest antimutagenic effect with over 90% against MNNG, 4NQO, B(a)P and Trp-P-l. However, ethyl acetate fraction (1 mg/mL) which had 90.8%, 94.3% and 83.5% inhibitory effect against on MCF-7, A549, AGS showed the strong cytotoxic effect compared to other samples.
Keywords
antimutagenicity; cytotoxicity; Inonotus obliquus;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Saitoh A, Sato C, Niiyama K. 1996. チャガカバノアナタケの 變異原性抑 制効果について. 道衛硏所報 第6集.
2 Kahlos K, Lesnau A, Lange W. 1996. Preliminary tests of antiviralactivity of two Inonotus obliquus strains. Fitoterapia Vol LX VII, p 4.
3 Miyazaki J, Oikawa N, Yamada H. 1977. Studies on fungal (Penicillium chrysogenum) polysaccharides. XX. Galactomannan of Cordyceps sinenesis (Lepi doptera). Chem Pharm Bull 25: 3324-3330.   DOI
4 Kahlos K, Kangas L, Hitunen R. 1987. Antitumour activity of some compounds and fractions from an n-hexane extract of Inonotus obliquus. Acta Pharm Fenn 96: 33-40.
5 Goro C, Jnuli H, Yukiko Y, Yoshiko A, Fumoko F. 1970. Fractionation and purification of the polysaccharides with masked antiumor activity, especially lentinan from Lentinus edodes (Berk.) Sing. (an edible mushroom). Cancer Res 30: 2776-2781.
6 Kahlos K, Hiltunen R. 1983. Identification of some lanostane type triterpenes from Inonotus obliquus. Acta Pharm Fenn 92: 220-224.
7 Kweon MH, Lim EJ, Sung HC. 1998. Studies on bioactive polysaccharide isolated from Agaricus bisporus. Agric Chem Biotechnol 41: 60-66.
8 Ludwiczak RS, Wrzeciono U. 1975. Forschungen uber die chemischen bestandteil de Inonotus obliquus. IV. Ergosterol Rocz Chem 34: 1701-1705.
9 Loviagina EV, Shivrina AN. 1962. On steroid compound of the Chaga fungus. Biobimija 27: 794-800.
10 Ji JH, Kim MN, Choi KP, Chung CK, Ham SS. 2000. Antimutagenic and cytotoxicity effect of Agaricus blazei Murill extracts. Korean J Food Sci 32: 1371-1378.
11 Yahagi T, Degawa M, Seino Y, Matsushima T, Nagao M, Sugimura T, Hashimoto Y. 1975. Mutagenicity of carcinogenic azo dyes and their derivatives. Cancer Letter 1: 91-97.   DOI   ScienceOn
12 Monks A, Scudiero D, Skehan P, Shoemaker R, Paul K, Bistica D, Hose C, Langley J, Cronise P, Vaigro-Wolff A, Gray-Goodrich M, Canpbell H, Mayo J, Body M. 1991. Feasibility of a high-flux anticancer drug screen using a diverse panel of cultured human cell lines. J Natl Cancer Inst 83: 757-766.   DOI
13 Kier L. 1961. Triterpenes of Poria obliqua. J Pharm Sci 50: 471-474.   DOI
14 Bulatov PK, Berezina MP, Jakimov PA. 1959. Tsaga ii ee letsebnoje primenie pri rake IV. Stadii, Leningarad. p 326.
15 Shivrina AN. 1967. Chemical characteristics of compounds extracted from Inonotus obliquus. Chem Abstr 66: 17271-17279.
16 Mizuno T, Zhuang C, Abe K, Okamoto H, Kiho T, Ukai S, Leclerc S, Meijer L. 1999. Antitumor and hypohlycemic activities of polysaccharides from the Sclerotia and Mycelia of Inonotus obliquus (Pers.: Fr.) Pil. (Aphyllophoromycetideae). Int J Med Mushrooms 1: 301-316.   DOI
17 Ichimura T, Watanave O, Maruyama S. 1998. Inhibition of HIV-1 protease by water-soluble lignin-like substance from an edible mushrum, Fuscoporia obliqua. Biosci Biotechnol Biochem 62: 575-577.   DOI   ScienceOn