Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antimutagenic and Cytotoxic Effects of Ethanol Extract from the Inonotus obliquus

차가버섯 분획물의 항돌연변이 활성 및 암세포 성장억제효과

  • 함승시 (강원대학교 식품생명공학부) ;
  • 오상화 (강원대학교 식품생명공학부) ;
  • 김영균 (국민대학교 임산공학과) ;
  • 신광순 (경기대학교 식품생명공학과) ;
  • 장현유 (한국국립농업대학 버섯학과) ;
  • 정국훈 (두산그룹 생명공학연구조합)
  • Published : 2003.10.01
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Abstract

This study was performed to determine the antimutagenic and cytotoxic effects of ethanol extract, ethylacetate fraction, water fraction and water soluble and insoluble polysaccharides I and II isolated from Inonotus obliquus using Ames test and SRB assay. Each sample itself did not show mutagenic effect. Among samples, the water unsoluble polysaccharides II in the presence of 50 $\mu\textrm{g}$/plate showed the strongest antimutagenic effect with over 90% against MNNG, 4NQO, B(a)P and Trp-P-l. However, ethyl acetate fraction (1 mg/mL) which had 90.8%, 94.3% and 83.5% inhibitory effect against on MCF-7, A549, AGS showed the strong cytotoxic effect compared to other samples.

차가버섯 에탄올 추출물과 에틸아세테이트 및 물 분획물 그리고 수용성 다당과 불용성 다당 I, II에 대하여 항돌연변이원성 실험과 암세포성장억제 실험을 행한 결과, 각 시료에서 돌연변이원성은 없는 것으로 나타났으며 직접변이원인 MNNG와 4NQO, 간접변이원인 B[a]P와 Trp-P-1 모두에서 시료농도 50$\mu\textrm{g}$/plate에서 불용성 다당 II가 90% 이상의 높은 억제효과를 나타내었다. 또한 암세포 성장 억제 실험에서는 에틸아세테이드 분획물에서 다른 분획이나 시료들보다 시료농도 4 mg/mL에서 A549, AGS, 그리고 MCF-7극 세포에 대하여 각각 90.8%, 94.3%, 83.5%의 높은 암세포 성장억제 효과를 나타내었다.

Keywords

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