• 제목/요약/키워드: Spontaneous Hypertensive rats(SHR)

검색결과 17건 처리시간 0.021초

Acute Effects of Korean Earthworm(Lamnodrilus gotai Hatai) Juice on Blood Pressure and Spontaneous Motor Activity in Spontaneously Hypertensive Rats

  • Wie, Myung-Bok;Song, Dong-Keun;Kim, Yung-Hi
    • 생약학회지
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    • 제24권3호
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    • pp.255-257
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    • 1993
  • We investigated the effects of single administration of Korean earthworm(Lamnodrilus gotai Hatai) juice (KEJ) on blood pressure and spontaneous motor activity in spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto rats. We report that KEJ lowered blood pressure and spontaneous motor activity in SHR.

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Heme Oxygenase-l Induced by Aprotinin Inhibits Vascular Smooth Muscle Cell Proliferation Through Cell Cycle Arrest in Hypertensive Rats

  • Choi, Hyoung-Chul;Lee, Kwang-Youn;Lee, Dong-Hyup;Kang, Young-Jin
    • The Korean Journal of Physiology and Pharmacology
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    • 제13권4호
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    • pp.309-313
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    • 2009
  • Spontaneous hypertensive rats (SHR) are an established model of genetic hypertension. Vascular smooth muscle cells (VSMC) from SHR proliferate faster than those of control rats (Wistar-Kyoto rats; WKY). We tested the hypothesis that induction of heme oxygenase (HO)-1 induced by aprotinin inhibits VSMC proliferation through cell cycle arrest in hypertensive rats. Aprotinin treatment inhibited VSMC proliferation in SHR more than in normotensive rats. These inhibitory effects were associated with cell cycle arrest in the G1 phase. Tin protoporphyrin IX (SnPPIX) reversed the anti-proliferative effect of aprotinin in VSMC from SHR. The level of cyclin D was higher in VSMC of SHR than those of WKY. Aprotinin treatment downregulated the cell cycle regulator, cyclin D, but upregulated the cyclin-dependent kinase inhibitor, p21, in VSMC of SHR. Aprotinin induced HO-1 in VSMC of SHR, but not in those of control rats. Furthermore, aprotinin-induced HO-1 inhibited VSMC proliferation of SHR. Consistently, VSMC proliferation in SHR was significantly inhibited by transfection with the HO-1 gene. These results indicate that induction of HO-1 by aprotinin inhibits VSMC proliferation through cell cycle arrest in hypertensive rats.

고cholesterol 식이 자발성 고혈압 백서(白鼠)에서의 가미방풍통성산(加味防風通聖散)이 고혈압에 미치는 영향 (Effects of Kamibangpungtongsungsan on Hypertension in Spontaneous Hypertensive Rats Fed a Hyperlipidemic Diet)

  • 김종원;조현경;유호룡;설인찬;김윤식
    • 대한한방내과학회지
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    • 제27권3호
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    • pp.617-628
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    • 2006
  • This experiment was performed to determine the effects of Kamibangpungtongsungsan on hypertension in spontaneous hypertensive rats fed a hyperlipidemic diet (H-SHR). The results are summarized as follows : 1. Kamibangpungtongsungsan significantly decreased the blood pressure and pulse of rats in H-SHR. 2. Kamibangpungtongsungsan significantly decreased the levels of Aldosterone in H-SHR. 3. Kamibangpungtongsungsan significantly decreased the levels of dopamine and epinephrine in H-SHR, but did not significantly reduce the levels of norepinephrine, in H-SHR. 4. Kamibangpungtongsungsan did not significantly reduce the levels of electrolytes in H-SHR. 5. Kamibangpungtongsungsan significantly decreased the TNF-$\alpha$ and IL-6 levels in H-SHR but the IL-10 level increase no significant. These results suggest that the Kamibangpungtongsungsan might be usefully applied for the treatment of hypertension with hyperlipidemia.

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대두 가수분해물에서 분리한 UF-peptide가 in vivo에서 자발성 고혈압 흰쥐의 혈압 강하에 미치는 영향 (Effect of Soybean Hydrolysate on Hypertension in Spontaneously Hypertensive Rats)

  • 유리나;박수아;정대균;남희섭;신재익
    • 한국식품영양과학회지
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    • 제25권6호
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    • pp.1031-1036
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    • 1996
  • 대두 가수분해물인 UF-peptide in vivo에서 혈압강하 효과를 나타낼 수 있는지를 조사하기 위해, 실험동물로서 고혈압 모델인 자발성 고혈압 흰쥐(spontaneously hypertensive rat, SHR)를 대상으로 UF-peptide를 0, 3, 10%가 되도록 첨가한 식이 UF-Peptide로 6주간 사육하여 SHR의 혈압강하에 미치는 영향, 혈중 지질 수준 및 혈관조직 중 angiotensin I converting enzyme(ACE) 활성 등을 조사하였다. 그 결과, UF-peptide는 자발성 고혈압쥐의 혈압을 강하시키는 효과가 있었으며, 이 효과는 혈중 총 콜레스테롤 및 중성 지질 등의 지질 개선작용, 흉부동맥의 ACE 활성 저해작용을 통해 발현되는 것으로 추정되었다.

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오약순기산과 가미오약순기산이 고혈압과 심박동수에 미치는 영향 (Effects of Oyaksoonkisan and Kamioyaksoonkisan on Hypertension and Pulse Rate)

  • 전성배;김병탁;임낙철;김성훈
    • 대한한의학회지
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    • 제18권1호
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    • pp.267-277
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    • 1997
  • Experiments were performed to determine the effects of the Oyacksunkisan(烏藥順氣散) and Kamioyacksunkisan(加味烏藥順氣散) liquid extract on the hypertension and the pulse rate in Sprague-Dawley rat(SDR) and Spontaneous Hypertensive rats(SHR). The results were obtained as follows ; 1. Blood pressure was significantly decreased firstday and 11th day after administration of Oyacksunkisan extract and pretreatment of phentolamine. 2. Blood pressure and pulse rate were regulated first day after administration of Kamioyacksunkisan and blood pressure was significantly decreased after pretreatment of clonidine and phentolamine. 3. Oyacksunkisan and Kamioyacksunkisan didn't show any significant changes of blood pressure and pulse rate after pretreatment of prdpranolol, hydralazine and verapamil From the above result, it was concluded that Oyacksunkisan(烏藥順氣散) and Kamioyacksunkisan(加味烏藥順氣散) could be applied effectively to the hypertension.

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Distributional Patterns of Phospholipase C Isozymes in Heart and Brain of Spontaneously Hypertensive and Normotensive Rats

  • Choi, Ji-Woong;Cho, Young-Jin;Cha, Seok-Ho;Lee, Kweon-Haeng;Lee, Sang-Bok
    • The Korean Journal of Physiology and Pharmacology
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    • 제1권4호
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    • pp.385-392
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    • 1997
  • The phospholipase C (PLC)-mediated intracellular signal transduction pathway is considered to be involved in the regulation of blood pressure. However, little information is available concerning the distributional and functional significance of PLC in the genetic hypertensive rats. As the first step of knowing the role of PLC on hypertension, we investigated the distribution of 6 PLC isozymes $(PLC-{\beta}1,\;-{\beta}3,\;-{\beta}4,\;-{\gamma}1,\;-{\gamma}2\;and\;-{\delta}1)$ in the heart and brain, which are concerned with hypertension, in the normotensive Wistar-Kyoto rat (WKY) and spontaneously hypertensive rat (SHR) using the western blotting and immunocytochemistry. The immunoreactivities of PLC isozymes in brain were detected, but there were no distributional and quantitative differences between the WKY and SHR. In the heart, but the immunoreactivities to $PLC-{\beta}1$ and $-{\gamma}2$ in the SHR were higher than those in WKY. In immunocytochemistry to confirm these western blotting data, $PLC-{\beta}1$ and $-{\gamma}2$ were localized in cardiac myocytes and the intensities of immunoreactivity in SHR were stronger than that in WKY. These results suggest that $PLC-{\beta}1$ and $-{\gamma}2$ would have possibility to concern with the establishment of spontaneous hypertension.

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시호가용골모려탕합청심연자음가감방(柴胡加龍骨牡蠣湯合淸心蓮子飮加減方)이 고혈압백서의 혈압 관련인자에 미치는 영향 (Effect of Sihogayonggolmoryeotanghabcheongsimyeonjaemgagambang on Hypertension in Spontaneous Hypertensive Rats)

  • 이성종;안정조;조현경;유호룡;설인찬;김윤식
    • 동의생리병리학회지
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    • 제24권3호
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    • pp.430-438
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    • 2010
  • This study was done to investigate the effect of SHCG on hypertension in spontaneous hypertensive rats. SHR was sensitized and challenged with Sihogayonggolmoryeotanghabcheongsimyeonjaemgagambang (SHCG) for 4 weeks. The 3 groups have 6 rats, respectively. Experimental group was treated with 56.7 mg/kg of SHCG orally and control group was treated with 56.7 mg/kg of normal saline instead. SHCG significantly showed safety against cytotoxicity on hFCs and toxicity in the liver. SHCG significantly decreased the blood pressure and the heart rate. SHCG significantly decreased the levels of aldosterone. SHCG significantly decreased the levels of dopamine, norepinephrine, and epinephrine. SHCG significantly decreased the levels of potassium and chloride. SHCG significantly decreased the levels of uric acid and creatinine. These results suggest that SHCG might be hopeful in treatment of hypertension.

천마구등음가감(天麻鉤藤飮加減)이 고혈압 유관인자 및 SHR 병태모델에 미치는 영향 (The Effect of Cheonmagudeng-um gagam(CGG) on Spontaneous Hypertensive Rat(SHR))

  • 송병용;최은희;정태산;강성순;안가영;김오영;전상윤;홍석
    • 대한한방내과학회지
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    • 제32권3호
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    • pp.345-360
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    • 2011
  • Objectives : This study was examined to investigate the effects of Cheonmagudeng-um gagam (CGG) extract on spontaneous hypertension. Methods : For the study of CGG, we divided rats into three groups. The normal group was Wister Kyoto rats (WKY). The control group was spontaneously hypertensive rats (SHR). The treatment group was SHR which were administered CGG extract (SHR-CGG). SHR-CGG were orally administered CGG extract that was diluted in distilled water at the various concentrations for 4 weeks (234.5 mg/kg) and SHR were orally administered the same dosage of plain distilled water as SHR-CGG. Then we measured anti-oxygen effects, ACE inhibitory activity, weight of heart and kidney, blood pressure, heart rate, plasma aldosterone, electrolyte, creatinine, uric acid, BUN, and observed the cortex of the cardiac muscle, kidney, and adrenal gland. Results : CGG increased DPPH scavenging activity and SOD similar activity depending on the concentration. CGG significantly decreased ROS, TNF-${\alpha}$, IL-6, IL-$1{\beta}$, heart weight, blood pressure, heart rate, aldosterone, and BUN in SHR. CGG increased ACE inhibition activity depending on the concentration. CGG inhibited the heart, kidney and adrenal gland tissue injury that is caused by hypertension. Conclusions : These results suggest that CGG is effective in treatment and prevention of hypertension.

Potentiation of endothelium-dependent vasorelaxation of mesenteric arteries from spontaneously hypertensive rats by gemigliptin, a dipeptidyl peptidase-4 inhibitor class of antidiabetic drug

  • Kim, Hae Jin;Baek, Eun Bok;Kim, Sung Joon
    • The Korean Journal of Physiology and Pharmacology
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    • 제22권6호
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    • pp.713-719
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    • 2018
  • Dipeptidyl peptidase4 (DPP4) inhibitors such as gemigliptin are antidiabetic drugs elevating plasma concentration of incretins such as GLP-1. In addition to the DPP4 inhibition, gemigliptin might directly improve the functions of vessels under pathological conditions. To test this hypothesis, we investigated whether the acetylcholine-induced endothelium dependent relaxation (ACh-EDR) of mesenteric arteries (MA) are altered by gemigliptin pretreatment in Spontaneous Hypertensive Rats (SHR) and in Wistar-Kyoto rats (WKY) under hyperglycemia-like conditions (HG; 2 hr incubation with 50 mM glucose). ACh-EDR of WKY was reduced by the HG condition, which was significantly recovered by $1{\mu}M$ gemigliptin while not by saxagliptin and sitagliptin up to $10{\mu}M$. The ACh-EDR of SHR MA was also improved by $1{\mu}M$ gemigliptin while similar recovery was observed with higher concentration ($10{\mu}M$) of saxagliptin and sitagliptin. The facilitation of ACh-EDR by gemigliptin in SHR was not observed under pretreatment with NOS inhibitor, L-NAME. In the endothelium-denuded MA of SHR, sodium nitroprusside induced dose-dependent relaxation was not affected by gemigliptin. The ACh-EDR in WKY was decreased by treatment with $30{\mu}M$ pyrogallol, a superoxide generator, which was not prevented by gemigliptin. Exendin-4, a GLP-1 analogue, could not enhance the ACh-EDR in SHR MA. The present results of ex vivo study suggest that gemigliptin enhances the NOS-mediated EDR of the HG-treated MA as well as the MA from SHR via GLP-1 receptor independent mechanism.

구기자 가공품의 생리기능성 및 항고혈압 활성 검증 (Physiological Functionality of Gugija Products and an In Vivo Examination on Anti-hypertension Effects)

  • 이지수;박영춘;백승우;이석수;안용근;이종수
    • 한국식품영양학회지
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    • 제21권2호
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    • pp.115-120
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    • 2008
  • To develop a new Gugija(Lycium chinensis Mill) product having increased value, the physiological functionality of various commercial Gugija products were investigated. In addition, an in vivo study was performed using spontaneous hypertensive rats(SHR) to examine the anti-hypertension effects of products. The results showed that antioxidant activity was highest in the methanol extract of Gugija leaf pickle(97.7%), and anti-hypertensive angiotensin I -converting enzyme(ACE) inhibitory activity was 80.4% in Gugija doenjang(soybean paste) water extract. Anti-cholesterolemia HMG-CoA reductase inhibitory activity was highest in the methanol extract of Gugija rice cake(66.1 %). However, SOD-like activity was below 30% in most products; and fibrinolytic activity was not detected or was very weak. Ultimately, we selected Gugija tea and Gugija wine as superior anti-hypertensive Gugija products, and subsequent in vivo testing was performed using SHR, comparing the tea and wine to Gugija fruit. Among them, the Gugija fruit demonstrated the best anti-hypertension effects in SHR.