• Title/Summary/Keyword: Spo2

Search Result 64, Processing Time 0.021 seconds

Expression and Regulatory Analysis of Sporulation Gene (spo 5) in Schizosaccharomyces pombe (Schizosaccharomyces pombe 포자형성유전자 (spo 5)의 발현조절기구의 해석)

  • KIM Dong-Ju;SHIMODA Chikasi
    • Korean Journal of Fisheries and Aquatic Sciences
    • /
    • v.30 no.1
    • /
    • pp.46-54
    • /
    • 1997
  • Sporulation in the fission yeast Schizosaccharomyces pombe has been regarded as an important model of cellular development and differentiation. S. pombe cells proliferate by mitosis and binary fission on growth medium. Deprivation of nutrients especially nitrogen sources, causes the cessation of mitosis and initiates sexual reproduction by malting between two sexually compatible cell types. Meiosis is then followed in a diploid cell in the absence of nitrogen source. DNA fragment complemented with the mutations of sporulation gene was isolated from the S. pombe gene library constructed in the vector, pDB 248' and designated as pDB (spo 5)1. We futher analyzed six recombinant plasmids, pDB (spo 5)2, pDB(spo 5)3, pDB(spo 5)4, pDB(spo 5)5, pDB(spo 5)6, pDB(spo 5)7, and found each plasmids is able to rescue the spo 5-2, spo 5-3, spo 5-4, spo 5-5, spo 5-6, spo 5-7, mutations, respectively. Mapping of the integrated plasmid into the homologous site of the S. pombe chromosomes demonstrated that pDB (spo 5)1, and pDB (spo 5)R1 contained the spo 5 gene. Transcipts of spo 5 gene were analyzed by Northern hybridization. Two transcripts of 3.2 kb and 25 kb were detected with 5 kb Hind III fragment containing a part of the spo 5 gene as a probe. The small mRNA (2.5 kb) appeared only when a wild-type strain was cultured in the absence of nitrogen source in which condition the large mRNA (3.2 kb) was produced constitutively. Appearance of a 2.5 kb spo 5-mRNA depends upon the function of the mei1, mei2 and mei3 genes.

  • PDF

Cloning and Transcription Analysis of Sporulation Gene (spo5) in Schizosaccharomyces pombe (Schizosaccharomyces bombe 포자형성 유전자(spo5)의 Cloning 및 전사조절)

  • 김동주
    • The Korean Journal of Food And Nutrition
    • /
    • v.15 no.2
    • /
    • pp.112-118
    • /
    • 2002
  • Sporulation in the fission yeast Schizosaccharomyces pombe has been regarded as an important model of cellular development and differentiation. S. pombe cells proliferate by mitosis and binary fission on growth medium. Deprivation of nutrients especially nitrogen sources, causes the cessation of mitosis and initiates sexual reproduction by matting between two sexually compatible cell types. Meiosis is then followed in a diploid cell in the absence of nitrogen source. DNA fragment complemented with the mutations of sporulation gene was isolated from the S. pombe gene library constructed in the vector, pDB 248' and designated as pDB(spo5)1. We futher analyzed six recombinant plasmids, pDB(spo5)2, pDB(spo5)3, pDB(spo5)4, pDB(spo5)5, pDB (spo5)6, pDB(spo5)7 and found each of these plasmids is able to rescue the spo5-2, spo5-3, spo5-4, spo5-5, spo5-6, spo5-7 mutations, respectively. Mapping of the integrated plasmid into the homologous site of the S. pombe chromosomes demonstrated that pDB(spo5)1, and pDB(spu5)Rl contained the spo5 gene. Transcripts of spo5 gene were analyzed by Northern hybridization. Two transcripts of 3.2 kb and 2.5kb were detected with 5kb Hind Ⅲ fragment containing a part of the spo5 gene as a probe. The small mRNA(2.5kb) appeared only when a wild-type strain was cultured in the absence of nitrogen source in which condition the large mRNA (3.2kb) was produced constitutively. Appearance of a 2.5kb spo5-mRNA depends upon the function of the meil, mei2 and mei3 genes.

Preparation and Photoluminescence Characteristics of Liquid Silicone Rubber Containing Cadmium Selenide Nanoparticles (Cadmium Selenide Nanoparticles을 함유하는 액상실리콘 고무의 제조와 형광특성)

  • Kang Doo-Whan;Lee Byoung-Chul;Kim Ji-Young
    • Polymer(Korea)
    • /
    • v.30 no.3
    • /
    • pp.266-270
    • /
    • 2006
  • Poly [(dimethylmethylyinyl) siloxane] phosphineoxide (PMViSPO) was prepared by adding phosphorus oxychloride $(POCl_3)$ to poly (dimethylmethylyinyl) siloxane (PMViS) at $0^{\circ}C$ under nitrogen atmosphere. Cadmium selenide (CdSe) was prepared by reacting cadmium oxide (CdO), tetradecyl-phosphonic acid (TDPA), trioctylphosphine oxide (TOPO) at $300^{\circ}C$, and adding solution of dissolved Se to tributylphosphine (TBP) and trioctylphosphine (TOP) CdSe-poly [(dimethylmethylvinyl) siloxane] phosphine-oxide (CdSe-SPO) adduct was synthesised by adding PMViSPO to CdSe solution. Liquid silicone rubber composite (LSRC-1) was prepared by compounding $\alpha,\omega-vinyl$ poly (dimethylsiloxane) (VPMS), $\alpha,\omega-hydrogen$) poly(dimethylsiloxane) (HPMS), and CdSe under Pt catalyst, and also LSRC-2 was prepared from VPMS, HPMS, and CdSe-SPO using Pt catalyst. It was confirmed that CdSe nanoparticles with photoluminescence characteristics was dispersed uniformly in LSR matrix. The diameter of CdSe was $30\sim50nm$. By measuring the number of CdSe nanoparticles, 202 particles of CdSe in LSRC-2 and 165 particles of CdSe in LSRC-1 were dispersed in the same area of LSR matrix. Thermal stability for LSRC-2 compounded with CdSe-SPO was better than LSRC-1.

REPRESENTATIONS FOR LIE SUPERALGEBRA spo(2m,1)

  • Lee, Chan-Young
    • Journal of the Korean Mathematical Society
    • /
    • v.36 no.3
    • /
    • pp.593-607
    • /
    • 1999
  • Let denote the orthosymplectic Lie superalgebra spo (2m,1). For each irreducible -module, we describe its character in terms of tableaux. Using this result, we decompose kV, the k-fold tensor product of the natural representation V of , into its irreducible -submodules, and prove that the Brauer algebra Bk(1-2m) is isomorphic to the centralizer algebra of spo(2m, 1) on kV for m .

  • PDF

Blood flow velocity measurement device using a pulse wave and ECG (맥파와 심전도를 이용한 혈류속도 측정장치)

  • Jeong, Jinhyung;Heo, Sun oh;Kim, Taegeun;Kim, Yunjeong;Lee, Kiyoung
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
    • /
    • v.6 no.1
    • /
    • pp.57-64
    • /
    • 2013
  • 본 논문에서는 심전도(ECG)와 SPO2를 이용하여 혈류속도를 측정하였다. 심전도(ECG)와 손가락 검지손가락 끝의 SPO2농도로 얻는 맥파를 동시에 측정하여 두 파형의 피크값의 시간차를 분석하여 혈류속도를 계산하는 방법을 연구하였다. 심전도(ECG)와 SPO2를 동시 측정 분석하는 시스템으로서 정상인 10명과 임상참가자 3명에 대한 심전도(ECG)와 손가락 검지 손가락 끝의 SPO2농도로 얻는 맥파를 동시에 측정하여 비교 분석한 결과 자료에서 보는 것과 같이, 심혈관계 환자들이 일반적으로 혈류 속도가 빠르다는 것일 볼 수 있다. 또한 정상인들 중에서도 남자와 여자의 속도차이가 있음을 알 수 있다.

Design and implementation of SPO2 wearable device for companion animals (반려동물용 SPO2 웨어러블 장치의 설계 및 구현)

  • Gong, i-Hyuk;Oh, Sang-Hyun;Kim, Dong-Uk;Kwak, Ho-Young
    • Proceedings of the Korean Society of Computer Information Conference
    • /
    • 2022.07a
    • /
    • pp.657-658
    • /
    • 2022
  • 본 논문에서는 반려동물용 산소포화도 측정 웨어러블 장치를 설계하고 구현하였다. 기존 장치들은 움직이는 반려동물들의 SPO2를 측정하는데에는 방법이 어렵거나 측정 오류가 발생하여 불편한 점이 있었다. 본 논문에서는 이러한 점을 개선하고 수의학적으로 측정 부위에 대한 새로운 위치를 찾아 가능한 형태의 웨어러블을 설계·구현하고 그 성능을 보였다.

  • PDF

Localization of MAK18 gene on chromosome VIII of saccharomyces cerevisiae (Saccharomyces cerevisiae의 염색체 VIII상의 MAK 18 유전자 국소화)

  • 윤순찬;이현숙;이창원
    • Korean Journal of Microbiology
    • /
    • v.26 no.4
    • /
    • pp.318-323
    • /
    • 1988
  • MAK18 gene of Saccharomyces cerevisiae, needed for M1 replication, was mapped within 2cM of PET3 on chromosome VIII. From 38kb clone pRE66 carrying SPO11 and PET3, we have localized MAK18 gene whose insert is 2.8kb. MAK18 gene is Iocalized on about 9kb distance from PET3 and about 18kb distance from SPO11 on chromosome VIII.

  • PDF

Studies on the Toxic Activity of Bacillus sphaericus Spo -D1216 Asporogenic Mutant (Bacillus sphaericus Asporogenic Mutant Spo - D1216의 독성에 관한 연구)

  • 복거중;김영한;이형환
    • Microbiology and Biotechnology Letters
    • /
    • v.13 no.2
    • /
    • pp.157-162
    • /
    • 1985
  • The cell cultures and crude extracts of Bacillus sphaericus 1593 K-5 and its mutant Spo-Dl216 were respectively bioassayed against Culex pipiens var. pollens mosquito larvae. The B. sphaeriucs 1593 K-5 showed toxic activity against the larvae. LC$_{50}$ values (cells/$m\ell$) was 2.6$\times$10$^2$. Also the LC$_{50}$ ($\mu\textrm{g}$ Protein/$m\ell$) of the crude extract was 10.26. However, B. sphaericus Spo-Dl216 didn't show toxic activity against the larvae. The soluble cytoplasmic toxin in broken B. sphaeriucs 1593k-5 cells was partially purified by gel permeation chromatography and ion exchange chromatography. Among the fractions of the gel permeation chromatography only a single fraction was found to be toxic. LC$_{50}$ values ($\mu\textrm{g}$ protein/$m\ell$) of the active fraction was 0.182. The active fraction of the gel permeation was subjected to ion exchange chromatography. Only a single fraction showed toxic activity and its LC$_{50}$ values ($\mu\textrm{g}$ protein/$m\ell$) was 0.02..02.

  • PDF

The Incidence and Management of Dural Tears and Cerebrospinal Fluid Leakage during Corrective Osteotomy for Ankylosing Spondylitis with Kyphotic Deformity

  • Jo, Dae-Jean;Kim, Ki-Tack;Lee, Sang-Hun;Cho, Myung-Guk;Seo, Eun-Min
    • Journal of Korean Neurosurgical Society
    • /
    • v.58 no.1
    • /
    • pp.60-64
    • /
    • 2015
  • Objective : To present the incidence and management of dural tears and cerebrospinal fluid leakage during corrective osteotomy [Pedicle Subtraction Osteotomy (PSO) or Smith-Petersen Osteotomy (SPO)] for ankylosing spondylitis with kyphotic deformity. Methods : A retrospective study was performed for ankylosing spondylitis patients with fixed sagittal imbalance, who had undergone corrective osteotomy (PSO or SPO) at lumbar level. 87 patients were included in this study. 55 patients underwent PSO, 32 patients underwent SPO. The mean age of the patients at the time of surgery was 41.7 years (21-70 years). Of the 87 patients, 15 patients had intraoperative dural tears. Results : The overall incidence of dural tears was 17.2%. The incidence of dural tears during PSO was 20.0%, SPO was 12.5%. There was significant difference in the incidence of dural tears based on surgical procedures ( PSO vs. SPO) (p<0.05). The dural tears ranged in size from 12 to $221mm^2$. A nine of 15 patients had the relatively small dural tears, underwent direct repair via watertight closure. The remaining 6 patients had the large dural tears, consequently direct repair was impossible. The large dural tears were repaired with an on-lay graft of muscle, fascia or fat harvested from the adjacent operation site. All patients had a successful repair with no patient requiring reoperation for the cerebrospinal fluid leak. Conclusion : The overall incidence of dural tears during PSO or SPO for ankylosing spondylitis with kyphotic deformity was 17.2%. The risk factor of dural tears was complexity of surgery. All dural tears were repaired primarily using direct suture, muscle, fascia or fat graft.

GENERATING SETS OF STRICTLY ORDER-PRESERVING TRANSFORMATION SEMIGROUPS ON A FINITE SET

  • Ayik, Hayrullah;Bugay, Leyla
    • Bulletin of the Korean Mathematical Society
    • /
    • v.51 no.4
    • /
    • pp.1055-1062
    • /
    • 2014
  • Let $O_n$ and $PO_n$ denote the order-preserving transformation and the partial order-preserving transformation semigroups on the set $X_n=\{1,{\ldots},n\}$, respectively. Then the strictly partial order-preserving transformation semigroup $SPO_n$ on the set $X_n$, under its natural order, is defined by $SPO_n=PO_n{\setminus}O_n$. In this paper we find necessary and sufficient conditions for any subset of SPO(n, r) to be a (minimal) generating set of SPO(n, r) for $2{\leq}r{\leq}n-1$.