• 한국초지조사료학회지
• /
• 제20권2호
• /
• pp.115-122
• /
• 2000

This study was carried out to determine a fertilization rate for productivity and nutritive value improvement of silage corn, using two kinds of composts, and to examine the potential possibility of utilization as an organic fertilizer. The experiment was conducted on the field plot at Gongiam, Kwangju, Kyunggi-Do for 3 years, from 1996 to 1998, and arranged in split-plot design with three replications. The main plots were two kinds of composts such as swine manure fermented with sawdust (SMFWS) and swine manure fermented without sawdust (SMF). Subplots were the nitrogen fertilization rates (0, 100, 200, 300 and 400kgN/ha/year). The dry matter (DM) yield increased as the nitrogen fertilization rate increased up to a rate of 300 kg N/ha, but decreased at rate of 400 kg N/ha. Dry matter yield in SMFWS treatment was higher than that of SMF treatment, but there was no significant difference between SMFWS and SMF treatments. Net energy for lactation (NEI) and total digestible nutrients (TDN) in corn increased as the fertilization rate of SMFWS and SMF increased, and crude protein (CP) content increased by the fertilization of SMFWS and SMF. No difference of CP, NEI and TDN was found between SMFWS and SMF treatments.

• 한국초지조사료학회지
• /
• 제25권1호
• /
• pp.17-22
• /
• 2005

This experiment was conducted to investigate the effect of seeding dates and rates on the productivity and nutritive value of hairy vetch(Vicia villosa Roth) at experimental field of Grassland and Forage Crops Division, National Livestock Research Institute, RDA, Suwon from 1999 to 2000. The experimental design was consisted of split-plot design with three replications. The main plot was seeding dates(25 Aug., 15 Sep. and 5 Oct.) and the subplot was seeding rates(20, 30 and 40 kg/ha). Late seeding dates increased the plant height and delayed the flowering stage as 1 or 2 days. The dry matter(DM) content was decreased with low seeding rates, and the trend was same in the all plots. Average crude protein(CP) content was $24.1\%$ and increased with delayed seeding date and lower seeding rate. The content of acid detergent fiber(ADF) and neutral detergent fiber(NDF) was increased with higher seeding rate, but seeding date did not affect. In vitro dry matter digestibility(IVDMD) of hairy vetch tended to decrease with high seeding rate, and total digestible nutrient(TND) did not show significant difference among seeding rates. Average relative feed value(RFV) of hairy vetch showed 140 which means very high quality. Dry matter and CP yield was decreased with delayed seeding date. The results of this experiments indicated that seeding in middle-August with 40 kg/ha would be recommended to produce the highest yield and quality f3r the cultivation of hairy vetch in middle part of Korea

• 한국식물학회:학술대회논문집
• /
• 한국식물학회 1999년도 Proceedings of the 17th Symposium on Plant Biology Environmental Stress and Photosynthesis
• /
• pp.83-90
• /
• 1999

Electron crystallography of two-dimensional crystalsand electron cryo-microscopy is becoming an established method for determining the structure and function of a variety of membrane proteins that are providing difficult to crystallize in three dimension. In this study this technique has been used to investigate the structure of a ~160 kDa reaction centre sub-core complex of photosystem II. Photosystem II is a photosynthetic membrane protein consisting of more than 25 subunits. It uses solar energy to split water releasing molecular oxygen into the atmosphere and creates electrochemical potential across the thylakoid membrane, which is eventually utilized to generate ATP and NADPH. Images were taken using Philips CM200 field emission gun electron microscope with an acceleration voltage of 200kW at liquid nitrogen temperature. In total, 79 images recorded dat tilt angles ranging from 0 to 67 degree yielded amplitudes and phases for a three-dimensional map with an in-plant resolution of 6$\AA$ and 11.4$\AA$ in the third dimension shows at least 23 transmembrane helices resolved in a monomeric complex, of which 18 were able to be assigned to the D1, D2, CP47 , and cytochrome b559 alfa beta-subunits with their associated pigments that ae active in electron transport (Rhee, 1998, Ph.D.thesis). The D1/D2 heterodimer is located in the central position within the complex and its helical scalffold is remarkably similar to that of the reaction centres not only in purple bacteria but also in plant photosystem I (PSI) , indicating a common evoluationary origin of all types of reaction centre in photosynthetic organism known today 9RHee et al. 1998). The structural homology is now extended to the inner antenna subunit, ascribed to CP47 in our map, where the 6 transmembrane helices show a striking structural similarity to the corresponding helices of the PSI reaction centre proteins. The overall arrangement of the chlorophylls in the D1 /D2 heterodimer, and in particular the distance between the central pair, is ocnsistent with the weak exciton coupling of P680 that distinguishes this reaction centre from bacterial counterpart. The map in most progress towards high resolution structure will be presented and discussed.

• 한국초지조사료학회지
• /
• 제26권2호
• /
• pp.97-104
• /
• 2006

본 연구는 수확기에 따른 호밀 품종간의 사초생산능력을 구명하기 위하여 수행하였다. 시험구는 분할구 배치법으로 주구는 4월 20일과 4월 30일의 수확시기를, 세구는 'Kodiak', 'Koolgrazer', 'Danko', 'Homil22', 'Olhomil' 품종으로 하였다. 조기수확시의 건물 함량은 각각 17.8%로 만기수확시의 20.0% 보다 낮았으나, 조단백질 및 TDN 함량에 있어서는 만기수확시 보다 높았다. 공시품종 간에는 건물, 조단백질 및 TDN 함량에 있어서 유의적인 차이가 있었다. ADF, NDF, ADL 및 cellulose 함량은 조기수확이 만기수확시 보다 낮았으나, hemi-cellulose 함량은 조기수확이 만기수확시 보다 높았다. 공시품종 간에는 조생품종의 섬유소함량이 다른 품종에 비하여 높았다. 조기수확시의 건물, 조단백질, IVDDM 및 TDN 수량은 각각 7,839, 1,468, 6,077 및 5,128kg/ha로 만기 수확시의 11,003, 1,813, 8.032 및 6,857kg/ha 보다 낮았으며 공시품종 간에는 조생품종이 다른 품종에 비하여 높았다. 시험에서 얻어진 결론은 사초용 호밀의 수확시기를 고려할 때 수량과 사료가치를 높일 수 있는 방법은 조생호밀품종에 만기수확을 조합하는 사초생산기술에 의해서 가능하다는 것이다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제26권10호
• /
• pp.1474-1483
• /
• 2013

The objective was to evaluate the use of prediction equations based on the chemical composition of feedstuffs to estimate the values of apparent metabolisable energy corrected for nitrogen balance (AMEn) of corn and soybean meal for broilers. For performance and carcass characteristics, 1,200 one-d-old birds (male and female) were allotted to a completely randomised factorial $2{\times}8$ (two genders and eight experimental diets) with three replicates of each sex with 25 birds. In the metabolism trial, 240 eight-d-old birds were distributed in the same design, but with a split plot in time (age of evaluation) with five, four and three birds per plot, respectively, in stages 8 to 21, 22 to 35, and 36 to 42 d of age. The treatments consisted of the use of six equations systems to predict the AMEn content of feedstuffs, tables of food composition and AMEn values obtained by in vivo assay, totalling eight treatments. Means were compared by Scott-Knott test at 5% probability and a confidence interval of 95% was used to check the fit of the energy values of the diets to the requirements of the birds. As a result of this study, the use of prediction equations resulted in better adjustment to the broiler requirements, resulting in better performance and carcass characteristics compared to the use of tables, however, the use of energy values of feedstuffs obtained by in vivo assay is still the most effective. The best equations were: AMEn = 4,021.8-227.55 Ash (for corn) combined with AMEn = -822.33+69.54 CP-45.26 ADF+90.81 EE (for soybean meal); AMEn = 36.21 CP+85.44 EE+37.26 NFE (nitrogen-free extract) (for corn) combined with AMEn = 37.5 CP+46.39 EE+14.9 NFE (for soybean); and AMEn = 4,164.187+51.006 EE-197.663 Ash-35.689 CF-20.593 NDF (for corn and soybean meal).

• Earthquakes and Structures
• /
• 제10권1호
• /
• pp.105-123
• /
• 2016

In this paper, using the probabilistic methods, the seismic demand of buckling restrained braced frames subjected to earthquake was evaluated. In this regards, 4, 6, 8, 10, 12 and 14-storybuildings with different buckling restrained brace configuration (including diagonal, split X, chevron V and Inverted V bracings) were designed. Because of the inherent uncertainties in the earthquake records, incremental dynamical analysis was used to evaluate seismic performance of the structures. Using the results of incremental dynamical analysis, the "capacity of a structure in terms of first mode spectral acceleration", "fragility curve" and "mean annual frequency of exceeding a limit state" was determined. "Mean annual frequency of exceeding a limit state" has been estimated for immediate occupancy (IO) and collapse prevention (CP) limit states using both Probabilistic Seismic Demand Analysis (PSDA) and solution "based on displacement" in the Demand and Capacity Factor Design (DCFD) form. Based on analysis results, the inverted chevron (${\Lambda}$) buckling restrained braced frame has the largest capacity among the considered buckling restrained braces. Moreover, it has the best performance among the considered buckling restrained braces. Also, from fragility curves, it was observed that the fragility probability has increased with the height.

• 한국초지조사료학회지
• /
• 제39권3호
• /
• pp.178-184
• /
• 2019

The objective of this study was to evaluate the dry matter yield of (DMY) of Italian ryegrass (IRG) forage crop based on two seeding date (September 25 and October 2) in 2017 and 2018, and evaluation of the potential reason of DMY reduction in IRG cultivars grown at Chuncheon, South Korea. Three cultivars of IRG including Kowinearly, Kowinmaster and Hwasan 104 were used in this experiment. The experimental field was designed as a split-split-plot arrangement. The cultivars were harvested on April 26 before the heading stage of both years. In this study, no significant effect (p>0.05) of seeding date has been found on the DMY, whereas the cultivars and year showed a significant effect on the DMY (p<0.05). Except September 25 of 2017, Hwasan 104 indicated significantly lower (p<0.05) DMY than Kowinearly and Kowinmaster in all seeding periods. Though no significant difference (p>0.05) was observed for the DMY between the two seeding dates in 2018, but the DMY was significantly lower (p<0.05) than that of 2017. Lower crude protein (CP) content of Kowinearly was observed on the seeding date of October 2 of 2017, and also in both seeding dates on 2018 (p<0.05). No significant difference (p>0.05) was observed in acid detergent fiber (ADF) between the cultivars on both seeding dates and years. Similarly, no significant difference (p>0.05) was found in neutral detergent fiber (NDF) between the cultivars in 2017. However, Kowinearly showed higher NDF content as compared to Kowinmaster and Hwasan 104 on both seeding dates and years. The seeding date has no significant difference (p>0.05). Thus, considering the extent of DMY in both years, the mean minimum temperature during winter season could be the limiting factor for the DMY of IRG cultivars. However, based on this study, the seeding dates of September 25 and October 2 have no potential effect on the DMY. Thus, on the basis of the current DMY and forge quality parameters, the cultivation Kowinmaster is recommended in Chuncheon.

• 한국초지조사료학회지
• /
• 제26권4호
• /
• pp.207-214
• /
• 2006

본 시험은 2002년 9월부터 2004년 12월까지 충남대학교 농업생명과학대학 내 부속 초지시험포장에서 수행하였다. 공시초종은 청예웅 호밀(Koolgrazer)과 헤어리베치(Common)를 공시하여 주구로 호밀 단파, 호밀 80%+헤어리베치 20% 혼파, 호밀 60%+헤어리베치 40%혼파 및 헤어리베치 단파 등의 4 파종유형과 세구로 수잉기, 출수기, 개화기 및 유숙기 등의 4 수확시기를 두어 분할구배치법 3반복으로 시험하였으며, 얻어진 결과는 다음과 같다. 2년 평f ha당 건물수량은 파종유형과 수확시기에 따라 차이를 보여, 호밀 단파구(9,776kg)가 호밀 80%+헤어리베치 20% 혼파구(9,045kg)나 호밀 60%+헤어리베치 40% 혼파구(8,272kg) 및 헤어리베치 단파구(5,424kg)에 비하여 높은 결과를 나타내었고, 모든 처리구에서 수확시기가 진행됨에 따라 유의적으로 증가되었다(p<0.01). 화학적 성분도 파종유형과 수확시기에 따라 차이를 보여, CP 함량과 DMD는 헤어리베치 단파구에서 높았던 반면에 섬유소물질의 함량은 가장 낮았으나, 호밀 단파구에서는 상반되는 결과를 보였다(p<0.01). 조단백질수량(CPDM)과 가소화건물(DDM)수량은 호밀 80%+헤어리베치 20% 혼파하여 개화기${\sim}$유숙기에 수확하는 것이 가장 높았다.(p<0.01). 이상의 결과로 보아, 호밀이나 혜어리베치의 단파 이용보다는 호밀에 헤어리베치를 20% 정도 혼파하여 개화기${\sim}$유숙기에 수확하는 것이 단위 면적당 수량과 사료가치를 높일 수 있는 한가지 방법이라 하겠다.

• 한국초지조사료학회지
• /
• 제28권3호
• /
• pp.237-244
• /
• 2008

본 연구는 사료작물 작부체계에 따른 액상분뇨 시용수준이 호밀과 호밀-레드 클로버의 생산성 및 지력증진에 미치는 영향을 조사하기 위한 것으로, 경기도 광주시 곤지암에서 2년간 수행 되었다. 작부체계(호밀단작, 호밀-레드 클로버 혼작)를 주구, 우분액비의 시용수준(0,150, 300 kg/N/ha)를 세 구로 하여 분할구 배치법 3반복으로 실험하였다. 파종량은 호밀-레드 클로버 혼작시 호밀 130 kg/ha, 레드 클로버 20 kg/ha을 매년 파종했으며, 호밀 단작시 150 kg/ha를 파종하여 수행하였다. 호밀단작과 호밀-레드 클로버 혼작에서 건물 생산량은 차이가 나타나지 않았으나 우분액비 시용이 증가함에 따라 그 생산성은 증가하였다(p<0.05). 호밀단작과 호밀-레드 클로버 혼작에서 조단백질 및 TDN 함량은 차이가 나타나지 않았으나 우분액비 시용이 증가함에 따라 조단백질 함량은 증가 하였다(p<0.05). 그러나 TDN 함량은 시용 수준 증가에 따른 영향이 거의 나타나지 않았다. NDF 및 ADF 함량은 작부체계와 우분액비 시용수준의 증가에 따라 거의 영향이 나타나지 않았다. 토양유기물 함량은 시험 시작 전 토양보다 시험 후 토양에서 현저하게 증가하였으며, 작부체계에 의한 영향은 나타나지 않았다. 그러나 우분액비 시용이 증가함에 따라서 현저하게 증가하였다(p<0.05).

• Asian-Australasian Journal of Animal Sciences
• /
• 제26권4호
• /
• pp.579-587
• /
• 2013

A total of 288 crossbred (Duroc${\times}$Landrace${\times}$Yorkshire) growing pigs were used in two experiments to investigate the effects of adding ${\beta}$-mannanase to corn-soybean meal-based diets on pig performance and apparent total tract digestibility (ATTD). Both experiments lasted 28 d and were split into two phases namely 1 to 14 days (phase 1) and 15 to 28 days (phase 2). In Exp. 1,144 pigs weighing $23.60{\pm}1.59$ kg BW were assigned to one of four corn-soybean meal-based diets containing 0, 200, 400 or 600 U/kg ${\beta}$-mannanase. Increasing the level of ${\beta}$-mannanase increased weight gain (quadratic effect; p<0.01) and feed efficiency (linear and quadratic effect; p<0.01) during the second phase and the overall experiment. However, performance was unaffected (p>0.05) by treatment during phase 1. Increasing the amount of ${\beta}$-mannanase in the diet improved (linear and quadratic effect; p<0.05) the ATTD of CP, NDF, ADF, calcium, and phosphorus during both phases. Based on the results of Exp. 1, the optimal supplementation level was determined to be 400 U/kg and this was the level that was applied in Exp. 2. In Exp. 2, 144 pigs weighing $23.50{\pm}1.86$ kg BW were fed diets containing 0 or 400 U/kg of ${\beta}$-mannanase and 3,250 or 3,400 kcal/kg digestible energy (DE) in a $2{\times}2$ factorial design. ${\beta}$-Mannanase supplementation increased (p<0.01) weight gain and feed efficiency while the higher energy content increased (p<0.01) feed intake and feed efficiency during both phases and overall. Increased energy content and ${\beta}$-mannanase supplementation both increased (p<0.05) the ATTD of DM, CP, NDF, ADF, phosphorus, and GE during both phases. There were no significant interactions between energy level and ${\beta}$-mannanase for any performance or digestibility parameter. In conclusion, the ${\beta}$-mannanase used in the present experiment improved the performance of growing pigs fed diets based on corn and soybean. The mechanism through which the improvements were obtained appears to be related to improvements in ATTD.