• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.32 no.3
• /
• pp.287-293
• /
• 1987

To find out the effects of seed maturity and seeding rate of two planting times on the seed emergence and silage and seed yields of rye, a local variety 'Paldanghomir' harvested 30 and 45 days after heading(DAH) were planted at five seeding rates on October(optimum) and November(late) in 1985. At the optimum planting, number of seedlings per unit area and number of tillers per plant were affected by seeding rate, but not by the seed maturities. However, at the late planting, number of seedlings per unit area and number of tillers per plant wer higher in the seed harvested 45 DAH, and they were not correlated with seeding rate in both seeds harvested 30 and 45 DAH. Heading date was 2 or 3 days earlier in the seeds harvested 45 DAH in both planting times. Silage yield was higher at the optimum planting and increased as seeding rate increased in both planting times. Number of spike and 1000~seed weight were higher at the optimum planting than late planting and higher in the seed harvested 45 DAH than 30 DAH in late planting, but were not different among seeding rates. Seed yield at optimum planting showed no difference between seed maturities and among seeding rates, and recommandable seeding rate was 600 seeds per $m^2$. At late planting the seed yield of the seed harvested 45 DAH in the seeding rate over 750 seeds per $m^2$ approached to the yield of the optimum planting. The seed harvested 30 DAH was unstable because of extremely low emergence rate and yield even at the higher seeding rate in late planting. planting.

• Journal of the Korean Applied Science and Technology
• /
• v.12 no.2
• /
• pp.121-130
• /
• 1995

This project has been worked out for isolation of EPA-producing bacteria from marine source of sea water, sea sediment and intestinal contents eviscerated from some red-muscle fish such as mackerel, horse-mackerel and spike fish. The samples were precultured on the media of PPES-II glucose broth and then pure-cultured on Nutrient agar and P-Y-M glucose. Lipids extracted from those bacterial mass collected by centrifugation were analysed in terms of lipid class and fatty acid composition. The results are resumed as follows : 1. 112 strains from sea water and 76 strains from sea sediment were tested for their EPA producing capability, but both strains of (SA-67 and SA-91) from the former and four strains(SS-35, 37, 51 and 71) from the latter have been proved to produce EPA above the level of 2% of total fatty acids. The strains such as GS-11, 29, 31, HM-9, 29, B-18, 33, 107, YL-129, 156, 203, 77, 104 and 256 which were isolated from fish intestinal contents, have also produced EPA at higher level than 2% of total fatty acids. 2. Contents of total lipids extracted from the cultures of these strains grown at $25^{\circ}C$, range from 2.8% to 6.9% (on dry weight %), and they are mainly composed of polar lipids($40.9{\sim}52.9%$) such as phosphatidyl glycerol($^{+}cardiolipin$)(?) and phosphatidyl ethanolamine ($33.8{\sim}40.0%$), with smaller amount of free fatty acid ($11.2{\sim}20.2%$). 3. EPA was isolated from a mixture of fatty acid methyl esters obtained from the lipid of each strain by HPLC in silver-ion mode and was identified by GC-Mass spectrometry. 4. The strains of SW-91, GS-11, GS-29, HM-9, B-18 and YL-203 grown at $25^{\circ}C$ have a level of 5% EPA in their total fatty acids, and the GS-11 and HM-9 strains show a tendency of increase in the EPA level with an increase of growth temperature.

• Microbiology and Biotechnology Letters
• /
• v.41 no.3
• /
• pp.272-277
• /
• 2013

In this study, the tar compounds derived from the plant cell cultures of Taxus chinensis were first identified and then quantified via gas chromatography/mass spectrometry (GC/MS) and gas chromatography (GC). 2-Picoline, 2,5-xylenol, acenaphthene, 1-methylnaphthalene and o-xylene were found to be the major tar compounds by biomass. These compounds were identified and confirmed by comparing their retention times with those of authentic compounds. Each compound also spiked with the pure standard. The contents of 2-picoline, 2,5-xylenol, acenaphthene, 1-methylnaphthalene, and o-xylene in biomass were 0.2512, 0.1586, 0.1240, 0.0942 and 0.0525 wt%, respectively. Liquid-liquid extraction and adsorbent treatment were able to remove 42% and 94% of the tars from biomass, respectivly. After hexane precipitation, all of the tars were perfectly removed.

• The Korean Journal of Physiology and Pharmacology
• /
• v.20 no.1
• /
• pp.101-109
• /
• 2016

Reducing $[Mg^{2+}]_o$ to 0.1 mM can evoke repetitive $[Ca^{2+}]_i$ spikes and seizure activity, which induces neuronal cell death in a process called excitotoxicity. We examined the issue of whether cultured rat hippocampal neurons preconditioned by a brief exposure to 0.1 mM $[Mg^{2+}]_o$ are rendered resistant to excitotoxicity induced by a subsequent prolonged exposure and whether $Ca^{2+}$ spikes are involved in this process. Preconditioning by an exposure to 0.1 mM $[Mg^{2+}]_o$ for 5 min inhibited significantly subsequent 24 h exposure-induced cell death 24 h later (tolerance). Such tolerance was prevented by both the NMDA receptor antagonist D-AP5 and the L-type $Ca^{2+}$ channel antagonist nimodipine, which blocked 0.1 mM $[Mg^{2+}]_o$-induced $[Ca^{2+}]_i$ spikes. The AMPA receptor antagonist NBQX significantly inhibited both the tolerance and the $[Ca^{2+}]_i$ spikes. The intracellular $Ca^{2+}$ chelator BAPTA-AM significantly prevented the tolerance. The nonspecific PKC inhibitor staurosporin inhibited the tolerance without affecting the $[Ca^{2+}]_i$ spikes. While $G{\ddot{o}}6976$, a specific inhibitor of $PKC{\alpha}$ had no effect on the tolerance, both the $PKC{\varepsilon}$ translocation inhibitor and the $PKC{\zeta}$ pseudosubstrate inhibitor significantly inhibited the tolerance without affecting the $[Ca^{2+}]_i$ spikes. Furthermore, JAK-2 inhibitor AG490, MAPK kinase inhibitor PD98059, and CaMKII inhibitor KN-62 inhibited the tolerance, but PI-3 kinase inhibitor LY294,002 did not. The protein synthesis inhibitor cycloheximide significantly inhibited the tolerance. Collectively, these results suggest that low $[Mg^{2+}]_o$ preconditioning induced excitotoxic tolerance was directly or indirectly mediated through the $[Ca^{2+}]_i$ spike-induced activation of $PKC{\varepsilon}$ and $PKC{\xi}$, JAK-2, MAPK kinase, CaMKII and the de novo synthesis of proteins.

• Korean Journal of Organic Agriculture
• /
• v.18 no.1
• /
• pp.75-82
• /
• 2010

The purpose of this study is to investigate an occurrence rate of rice stripe virus disease under different rice transplanting time and different rice cultivars. After final harvest, the yield of rice infected by rice stripe virus was obtained on Wangchal cultivar compared to uninfected rice. The results obtained as following: 1) Yield of rice infected by rice stripe virus was decreased by 64% with reduction of culm length, pancile length, number of spike, number of spikelet, and grain filling compared to uninfected rice, 2) An occurrence rate of rice stripe virus disease under different rice planting season was high as order of May 30 > June 15 > July 1. In additional, to reduce a rice stripe virus disease on higher disease occurrence region and susceptible cultivars, rice planting season will be accepted on and after June 15, and 3) Eighteen cultivars were resistance cultivars such as Hwayeongbyeo, Onnuri, Ilmibyeo, Nampyongbyeo, Dongjin2ho, Hopumbyeo, Hwangkeumnuri, Malgeumi, Saenuri, Pungmi1ho, Haechanmulgyeo, Hwangkeumnodeul, Chilbobyeo, Dongjinbyeo, Younganbyeo, Junambyeo, Samkwangbyeo, and Nakdongbyeo. Twenty-one cultivars were susceptible cultivars such as Odaebyeo, Unkwangbyeo, Shinungbong1ho, Manabyeo, Hopyongbyeo, Borami, Damibyeo, Hwangchalbyeo, Sulgengbyeo, Nongrim6ho, Sasanishigi, Yunishigari, Chungmubyeo, Dongjin1ho, Huknambyeo, Guromy, Shanghehanghulna, Heukchalbyeo, Heukhwangbyeo, and Aranghwangchalbyeo.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.27 no.4
• /
• pp.263-268
• /
• 2007

This experiment was carried out to evaluate the effects of organic content and to select lodging resistance for six varieties in paddy field of Chungnam province from 2006 to 2007. In this experiment, plant height was increased at the high fertile field and dry matter rate (%) per fresh matter was decreased at the high fertile soil condition but dry matter rate (%) of spike was increased. The dry matter (DM) yield showed higher in Sunwoo barley (13,520kg/ha) and Youngyang barley (12,780kg/ha) at the high fertile field. Fresh yield was also high in Wooho barely (34,720kg/ha) and Youngyang barely (33,670kg/ha) at the high fertile field but yield in high and normal fertile soil condition was not difference. Total digestible nutrients (TDN) content of Youngyang barley and Yuyeon barley was high as 69.5%, 70.3%, respectively, at the high fertile soil condition. According to this result Soman barley and Youngyang barley might be recommendable for whole crop barely in lower organic content condition of paddy rice field and Wooho barley and Soman barley with the lodging resistance and high yield is suitable for paddy field with high organic content.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.29 no.3
• /
• pp.254-260
• /
• 1984

As a basic study for development of early wheats an experiment was carried out at four sites of Korea by use of 60 varieties. Analysis of variance for heading and maturing dates revealed that there were highly significant among varieties, but not significant among variety x location-interactions, thus indicating the stable response for earliness at all locations of each variety. Grain filling periods of each variety showed the stable response at all locations like those of heading and maturing dates, however, there showed significance among locations for early group, and among varieties for late group. High correlations were between growth habit and winter survival for early varieties, and between shortday length and grain filling period for late one. Interior factors most closely related to heading date were growth habit and winter survival, and winter survival showed highly negative correlation with heading date. It needs to breed the early wheats adaptable for southern and/or northern areas separatively. The earlier heading and maturing dates of wheats were, the shorter internodes of culm are, appearing the outstanding phenomenon in early wheats. Heading date appeared negative correlations with test weight and with number of culm before winter, but showed positive correlation with number of kernels/spike.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.37 no.2
• /
• pp.155-165
• /
• 1992

Experiments were conducted to determined the development of the vascular bundles in the peduncle of different tillers on its development in order to improve the vascular system and possibly increase grain yield. The development of the vascular bundle in the leaf, stem and panicle is an important aspect of assimilate translocation and differentiation of panicle characters. Two cultivars were used in this study: IR58, an indica type, and Unbong 7, a japonica type. The main culm(M) had more and bigger vascular bundles in the peduncle and those vascular bundle decreased with tiller order and tiller development. In the primary tillers, P1 had more large and small vascular bundles than P5 in both cultivars. IR58 developed more large vascular bundles compared to Unbong 7, but the small vascular bundle in unbong 7 was more than in IR58. The cross sectional area of phloem and xylem in large vascular bundle decreased with tiller order in both cultivar. Larger area of phloem and xylem in the early formed tillers more efficient transport of assimilates. The number of spikelets, the weight of panicle and grain yield per panicle were highest in the main culm followed by the order of their initiation or emergence. The number of primary and secondary branches to be positive associated with the number and area of vascular bundles. Furthermore, the number of vascular bundles in the peduncle was highly correlated with the peduncle thickness which in turn was correlated with the number of primary and secondary branches on the panicle. These results showed tillers that are initiated early and have relatively ation usually have more vascular bundles, larger peduncle, more spikelets spike let filling and ultimately higher yield.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.27 no.1
• /
• pp.41-48
• /
• 1982

A comparison was made for growth and yield of two rowed and six rowed barley varieties to differentiate their fundamental characteris in relation with brewing quality. Heading and maturing time of two rowed barley varieties were similar to those of six rowed covered but were earlier than those of six rowed naked ones. Most of two rowed varieties were spring barley and low temperature susceptible, but they were relatively photoinsensitive compared to six rowed varieties. Eventhough malting barley has heavier stands and grains, they were quite similar in yielding ability with six rowed cultivars with decreased number of grains per spike. In quality characters, malting barley has better germination energy and rate, lower protein content than the common six rowed barley and decisively better for brewing that six rowed barley with higher malt yield, extract yield and quality and enzyme activity. In comparison of malting barley varieties produced in Korea and Japan indicated that those varieties produced in Japan were better in germination characters, extract yield and quality, Kolback index and enzyme activity than those produced in Korea.

• Journal of Food Hygiene and Safety
• /
• v.18 no.3
• /
• pp.95-100
• /
• 2003

Competitive direct enzyme-linked immunosorbent assay(cdELISA) of aflatoxin $B_1$ ($AFB_1$) in deonjang(Korean-style soybean paste) and kochujang(fermented hot peppersoybean paste) and the level of $AFB_1$ in modern or traditional style deonjang and gochujang, produced in Korea, was surveyed by cdELISA. From the standard curve of the cdELISA, the detection limit of $AFB_1$ was 0.2 ng/m/. The average recovery of $AFB_1$ was 71.5% in the range of 1~100 ng/g after spiking $AFB_1$ into deonjang and it means that it could be possible to detect the $AFB_1$ in these range by the cdELISA in deonjang. Among the 30 kochujangs tested, no $AFB_1$ was detected in kochujangs. Among the 30 deonjangs, $AFB_1$ was detected in 6 ones in the range of 1.0~6.0 ng/g. The occurrence of $AFB_1$ in deonjang and kochujang tested in this study was less than the Korea Standard and Specification of aflatoxin in foods (10 ppb).