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http://dx.doi.org/10.4014/kjmb.1303.03001

Identification and Quantification of Tar Compounds in Plant Cell Cultures of Taxus chinensis  

Kim, Gun-Joong (Department of Chemical Engineering, Kongju National University)
Park, Gyu-Yeon (Department of Chemical Engineering, Kongju National University)
Kim, Jin-Hyun (Department of Chemical Engineering, Kongju National University)
Publication Information
Microbiology and Biotechnology Letters / v.41, no.3, 2013 , pp. 272-277 More about this Journal
Abstract
In this study, the tar compounds derived from the plant cell cultures of Taxus chinensis were first identified and then quantified via gas chromatography/mass spectrometry (GC/MS) and gas chromatography (GC). 2-Picoline, 2,5-xylenol, acenaphthene, 1-methylnaphthalene and o-xylene were found to be the major tar compounds by biomass. These compounds were identified and confirmed by comparing their retention times with those of authentic compounds. Each compound also spiked with the pure standard. The contents of 2-picoline, 2,5-xylenol, acenaphthene, 1-methylnaphthalene, and o-xylene in biomass were 0.2512, 0.1586, 0.1240, 0.0942 and 0.0525 wt%, respectively. Liquid-liquid extraction and adsorbent treatment were able to remove 42% and 94% of the tars from biomass, respectivly. After hexane precipitation, all of the tars were perfectly removed.
Keywords
Plant cell culture; Taxus chinensis; tar compounds; identification; quantification;
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1 ElSohly HN, Jr. Croom EM, ElSohly MA, McChesney JD. 1997. Methods and compositions for isolating taxanes. 1997. U.S. Patent 5,618,538.
2 Brage C, Sjostrom K. 1991. Separation of phenols and aromatic hydrocar bons from biomass tar using aminopropylsilane normal-phase liquid chromatography. J. Chromatogr. 2: 303-310.
3 Carpenter DL, Deutch SP, French RJ. 2007. Quantitative measurement of biomass gasifier tars using a molecularbeam mass spectrometer : Comparison with traditional impinger sampling. Energy & Fuels. 21: 3036-3043.   DOI
4 Choi HK, Adams TL, Stahlhut RW, Kim SI, Yun JH, Song BK, et al. 1999. Method for mass production of taxol by semi-continuous culture with Taxus chinensis cell culture. U.S. Patent 5,871,979.
5 Gamborg OL, Miller RA, Ojima K. 1968. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 151-158.   DOI
6 Han J, Kim HJ. 2008. The reduction and control technology of tar during biomass gasification/pyrolysis: An overview. Renew. Sustain. Energy Rev. 12: 397-416.   DOI
7 Han MK, Jeon KY, Mun S, Kim JH. 2010. Develoment of a micelle-fractional precipitation hybrid process for the pre-purification of paclitaxel from plant cell cultures. Process Biochem. 45: 1368-1374.   DOI
8 Hata H, Saeki S, Kimura T, Sugahara Y, Kuroda K. 1999. Adsorption of taxol into ordered mesoporous silicas with various pore diameters. Chem. Mater. 11: 1110-1119.   DOI
9 Jeon KY, Kim JH. 2008. Effect of surfactant on the micelle process for the pre-purification of paclitaxel. Korean J. Biotechnol. Bioeng. 23: 557-560.
10 Kim JH, Kang IS, Choi HK, Hong SS, Lee HS. 2000. Fractional precipitation for paclitaxel pre-purification from plant cell cultures of Taxus chinensis. Biotechnol. Lett. 22: 1753-1756.   DOI
11 Jeon KY, Kim JH. 2009. Improvement of fractional precipitation process for pre-purification of paclitaxel. Process Biochem. 44: 736-741.   DOI
12 Kim JH. 2006. Paclitaxel : recovery and purification in commercialization step. Korean J. Biotechnol. Bioeng. 21: 1-10.
13 Kim JH. 2009. Optimization of liquid-liquid extraction conditions for paclitaxel separation from plant cell cultures. KSBB J. 24: 212-215.
14 Kim JH, Kang IS, Choi HK, Hong SS, Lee HS. 2002. A novel prepurification for paclitaxel from plant cell cultures. Process Biochem. 37: 679-682.   DOI
15 Lee JY, Kim JH. 2011. Development and optimization of a novel simultaneous microwave-assisted extraction and adsorbent treatment process for separation and recovery of paclitaxel from plant cell cultures. Sep. Puri. Technol. 80: 240-245.   DOI
16 McGuire WP, Rowinsky EK, Resenshein NB, Grumbine FC, Ettinger DS, Armstrong DK, et al. 1989. Taxol : a unique antineo plastic agent with significant activity in advanced ovarian epithelial neoplasms. Ann. Intern. Med. 111: 273-279.   DOI
17 Oh HJ, Jang HR, Jung KY, Kim JH. 2012. Evaluation of adsorbents for separation and purification of paclitaxel from plant cell cultures. Process Biochem. 47: 331-334.   DOI
18 Pyo SH, Park HB, Song BK, Han BH, Kim JH. 2004. A largescale purification of paclitaxel from cell cultures of Taxus chinensis. Process Biochem. 39: 1985-1991.   DOI
19 Park YJ, Sirny RJ. 1969. Gas-liquid chromatographic determination of amino acids in some Korean foods. J. Korean Soc. Appl. Biol. Chem. 12: 43-51.
20 Pyo SH, Song BK, Ju CH, Han BH, Choi HJ. 2005. Effects of adsorbent treatment on the purification of paclitaxel from cell cultures of Taxus chinensis and yew tree. Process Biochem. 40: 1113-1117.   DOI
21 Rao KV. 1997. Method for the isolation and purification of taxol and its natural analogues. U.S. Patent 5,750,709.
22 Rowinsky EK, Cazenave LA, Donechower RC. 1990. Taxol : a novel investigational antimicrotubule agent. J. Natl. Cancer Inst. 82: 1247-1259.   DOI
23 Sim HA, Lee JY, Kim JH. 2012. Evaluation of a high surface area acetone/ pentane precipitation process for the purification of paclitaxel from plant cell cultures. Sep. Puri. Technol. 89: 112-116.   DOI
24 Uruska I, Koschmidder M. 1987. A calorimetric study of complex formation between molecular iodine and pyridine or 2- methylpyridine in weakly polar solvents. J. Chem. Soc., Perkin Trans. 2. 12: 1713-1715.