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Survey of Aflatoxin B1 in Domestic Doenjang and Kochujang Determined by Enzyme Linked-Immunosorbent Assay  

Bae, Soo-Ick (Korea Food Research Institute)
Kwak, Bo-Yeon (Korea Food Research Institute)
Park, Yun-Kyung (Korea Food Research Institute)
Kim, Young-Ho (Korea Food Research Institute)
Shon, Dong-Hwa (Korea Food Research Institute)
Publication Information
Journal of Food Hygiene and Safety / v.18, no.3, 2003 , pp. 95-100 More about this Journal
Abstract
Competitive direct enzyme-linked immunosorbent assay(cdELISA) of aflatoxin $B_1$ ($AFB_1$) in deonjang(Korean-style soybean paste) and kochujang(fermented hot peppersoybean paste) and the level of $AFB_1$ in modern or traditional style deonjang and gochujang, produced in Korea, was surveyed by cdELISA. From the standard curve of the cdELISA, the detection limit of $AFB_1$ was 0.2 ng/m/. The average recovery of $AFB_1$ was 71.5% in the range of 1~100 ng/g after spiking $AFB_1$ into deonjang and it means that it could be possible to detect the $AFB_1$ in these range by the cdELISA in deonjang. Among the 30 kochujangs tested, no $AFB_1$ was detected in kochujangs. Among the 30 deonjangs, $AFB_1$ was detected in 6 ones in the range of 1.0~6.0 ng/g. The occurrence of $AFB_1$ in deonjang and kochujang tested in this study was less than the Korea Standard and Specification of aflatoxin in foods (10 ppb).
Keywords
Aflatoxin $B_1$; deonjang; kochujang; ELISA;
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Times Cited By KSCI : 1  (Citation Analysis)
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