• Title/Summary/Keyword: Sperms

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An Experimental Study on the Effect of the Epididymal Biopsy on the Obstruction of the Epididymal Ducts (부고환 생검이 부고환의 폐색에 미치는 실험적 연구)

  • Kim, Du-Cheun;Kim, Sae-Chul
    • Clinical and Experimental Reproductive Medicine
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    • v.11 no.2
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    • pp.77-81
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    • 1984
  • Epididymal biopsy has been performed without consideration of the possibility of epididymal ductal severance and obstruction which result in obstructive azoospermia. An attempt was made to study the effect of the epididymal biopsy on the obstruction of the epididymal ducts. Bilateral epididymal biopsies were done in 8 healthy rabbits (New Zealand White strain) weighing over 3 kg, and then ejaculated semens have been analyzed 5 times every other week from 1 month after biopsies. Microscopic examination of the biopsied epididymides was also done after the 5th semen analysis. The results were as follows. 1. Semen analysis: 6 out of 8 rabbits showed azoospermia from the 4th semen analysis and 2 cases showed normal number of the sperms in the 5th semen analysis. 2, Microscopic examination: 6 cases of azoospermia showed complete obstruction of the biopsied sites of the epididymides, and abscence of sperms in epididymal ducts distal to the biopsied sites of the epididymides. However, recanalization of the epididymal ducts was noted in 2 cases showing normal sperm count. Therefore, it is concluded that the epididymal biopsy should be avoided in patients who want to be fertile, because it may cause the epididymal severance and obstruction of the epididymal ducts.

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REACTION TIME, SEMEN OUTPUT AND SEMEN QUALITY OF BUFFALO BULLS AFTER PRE-COLLECTION INJECTION OF PROSTAGLANDIN F2 ALPHA (PGF2 ALPHA)

  • Capitan, S.S.;Antiporda, G.S.;Momongan, V.G.
    • Asian-Australasian Journal of Animal Sciences
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    • v.3 no.4
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    • pp.343-346
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    • 1990
  • A study was conducted to determine the effect of prostaglandin $F_2$ alpha ($PGF_2$ alpha) on the reaction time and seminal characteristics of buffalo bulls. Semen was collected from three Murrah bulls in three periods: pre-treatment, treatment and post-treatment. During the treatment period each bull was administered 2 ml $PGF_2$ alpha (Synchrocept, Fenprostalene) im, 1 hour prior to semen collection. In the post-treatment, semen was collected 7 days after the last injection of $PGF_2$. Semen samples were evaluated immediately after collection. Pre-collection injection of $PGF_2$ alpha has no significant effect on reaction time, semen volume, percentage motility, sperm concentration and total number of sperms per ejaculate. Fluctuations in semen color and consistency were observed. There is a significant (p<0.05) increase in the mean percentage of normal spermatozoa during the treatment and post treatment periods. Likewise, administration of PG results into a significant (p<0.05) rise on the average percentage of live sperms but this effect was not manifested in the post-treatment period. Improvement in mass activity was observed during the treatment and post-treatment periods.

Effectiveness of Permanent Silicone Stent-Assisted Vasovasostomy (정관내 부목을 이용한 정관복원술 : Permanent Silicone Stent의 효용가치)

  • Kim, Tae-Hyoung;Kim, Sae-Chul
    • Clinical and Experimental Reproductive Medicine
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    • v.23 no.1
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    • pp.67-71
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    • 1996
  • During a 2-year period microsurgical vasovasostomies using permanent silicone stent(c-shaped stent with 0.6mm slit, 5mm in length, 0.8mm in outer diameter, 0.5mm in inner diameter) were performed in 30 patients for vasectomy revesal. The stent-assisted vasovasostomy(SVV) was intended to decrease the technical demand, the time requirement and the occurrence of reobstruction due to postoperative stricture. The effectiveness of the permanent silicone stent for vasovasostomy was compared with that of microsurgical two-layered vasovasostomy(VV). Sperms were present in all the ejaculates of the 25 men on semen analysis 1-2 months after SVV. Pregnancy occurred in 10 of 22 couples(45.5%) during 2-24 months of follow-up and it took 4-10 months(average 7.2 months) to get pregnant. Average operation time for the VV was 150 minutes and that for the SVV 125 minutes. Among 12 men who had sperms in the ejaculates 1-2 months after SVV, 4 revealed azoospermia 3-17 months postoperatively. Among the 4 patients with postoperative azoospermia, 2 underwent reoperation. On histopathologic examination, previously anastomsed sites showed obliterated lumen of the stent and strictures of vas proximal and/or distal to the stents due to granulation tissues. In conclusion, the SVV was not more efficacious in terms of patency and pregnancy rate than the VV.

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Analysis of Motional Characteristics of Sperm Using Image Processing (영상처리를 이용한 정자의 운동 특성 분석)

  • Shim, Hoon-Sup;Yi, Won-Jin;Park, Kwang-Suk;Paick, Jae-Seung
    • Journal of the Korean Institute of Telematics and Electronics B
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    • v.31B no.11
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    • pp.109-115
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    • 1994
  • In this paper, we developed an analyzing method of the motional characteristics of sperm, using image processing technology. Without the aid of a dedicated image-processor, this processing of a personal computer(PC) and a simple image processing board. The image processing board is used for acquiring images from a microscopic imaging source. The PC processes the images from the board and computes the parameters of motional characteristics of sperms. The algorithm of the site detection of sperms and the 'Match Matrix Method' is noteworthy. After comparing the results of our method with those of the manual method, and with those of the method using a dedicated image-processor, we concluded that our method is useful and reliable.

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A Simple Confocal Microscopy-based Method for Assessing Sperm Movement

  • Kim, Sung Woo;Kim, Min Su;Kim, Chan-Lan;Hwang, In-Sul;Jeon, Ik Soo
    • Development and Reproduction
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    • v.21 no.3
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    • pp.229-235
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    • 2017
  • In the field of reproductive medicine, assessment of sperm motility is a key factor for achieving successful artificial insemination, in vitro fertilization, or intracellular sperm injection. In this study, the motility of boar sperms was estimated using real-time imaging via confocal microscopy. To confirm this confocal imaging method, flagellar beats and whiplash-like movement angles were compared between fresh and low-temperature-preserved ($17^{\circ}C$ for 24 h) porcine sperms. Low-temperature preservation reduced the number of flagellar beats from $11.0{\pm}2.3beats/s$ (fresh sperm) to $5.7{\pm}1.8beats/s$ and increased the flagellar bending angle from $19.8^{\circ}{\pm}13.8^{\circ}$ (fresh) to $30.6^{\circ}{\pm}15.6^{\circ}$. These data suggest that sperm activity can be assessed using confocal microscopy. The observed motility patterns could be used to develop a sperm evaluation index and automated confocal microscopic sperm motility analysis techniques.

Dose dependent effect of benzene extract of Ocimum sanctum leaves on cauda epididymal spermatozoa of albino rats

  • Ahmed, Mukhtar;Ahamed, R. Nazeer;Aladakatti, R.H.;Ghodesawar, M.G.
    • Advances in Traditional Medicine
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    • v.9 no.4
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    • pp.339-349
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    • 2009
  • An attempt has been made to assess whether the dose dependent effect of benzene extract of Ocimum sanctum leaves on the morphological changes in the cauda epididymal spermatozoa and sperm parameters in male albino rats. Scanning Electron Microscope observations illustrate the disturbance in plasma membrane as well as acrosomal membrane. Most of the sperms appear morphologically abnormal in the mid region of the tail; there is formation of balloon like cytoplasmic droplet. Sperm parametric study exhibits decrease in the total sperm count, sperm motility, forward velocity and increase in the percentage of abnormal sperms in dose dependent manner on treatment benzene extract of Ocimum sanctum leaves. The results suggest that the effects may have resulted from a general disturbance in the proteins and alteration in cauda epididymal milieu probably due to androgen deficiency consequent upon antiandrogenic property of Ocimum sanctum leaves.

Cock Spermatozoa Serve as the Gene Vector for Generation of ransgenic Chicken (Gallus gallus)

  • Yang, C.C.;Chang, H.S.;Lin, C.J.;Hsu, C.C.;Cheung, J.I.;Hwu, L.;Cheng, W.T.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.7
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    • pp.885-891
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    • 2004
  • To evaluate the feasibility of using sperm-mediated gene transfer (SMGT) for carrying foreign gene into chicken oocyte, a reporter gene, CX-EGFP, was used in this study. The reporter gene was first mixed with liposome or liposome-like compound and the mixtures were further combined with ejaculated cock spermatozoa. The spermatozoa treated with liposome and CX-EGFP mixture was subsequently coincubated with DNaseI to remove the extra DNA which insured the authenticity of positive signals. The treated sperms were then subjected to transgene (reporter gene) existence analysis and artificial insemination of laying hens. Obtained results indicated that the spermatozoa were able to take-in the foreign DNA; which was confirmed by polymerase chain reaction and Southern blot analysis. In the following experiment, fresh ejaculated sperms were mixed with CX-EGFP-liposome or CX-EGFP-liposome-like complex then used for artificial insemination of each of six laying hens. Eggs laid between day-3 and day-7 post insemination were collected. Newly hatched chicks, two out of 53 from CX-EGFP/liposome treated group and two out of 21 from CXEGFP/liposome-like treated group, were proven to be transgenic. This study suggests that SMGT is a powerful method for generating transgenic chickens.

Effect of Multiple Showering and Vitamin Supplementation on Sexual Behaviour, Quality and Freezability of Buffalo Bull Semen

  • Singh, Pawan;Sengupta, B.P.;Tripathi, V.N.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.2
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    • pp.184-188
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    • 2001
  • In a summer study during May to July, involving 12 young Murrah buffalo bulls at forty months of average age, the effects of multiple shower vs single shower body cooling and vitamin A, D and E supplementation on the sexual behaviour, semen quality and freezability were investigated. The animals were divided into two groups (6 animals in each group) and housed in a half-walled shed with proper spacing, the feeding management being identical. The bulls in the control group were given a single shower at 1000 h, whereas the experimental bulls were given four showers at 10,12,14 and 16 h. In addition, the experimental bulls were given vitamin A, D and E injections at fifteen day intervals. The sexual behaviour of bulls was observed in terms of reaction time, sexual aggressiveness and ejaculatory thrust. Semen quality of all the bulls was assessed in terms of volume, mass activity, live-dead sperm and sperm concentration, sperm motility and morphology, and acrosomal abnormality. The sexual behaviour did not vary significantly between the groups, whereas semen quality differed significantly for volume, per cent live sperms, total sperms per ejaculate and total live sperm per ejaculate between groups. It can be concluded that sexual behaviour was not influenced by the thermal comfort treatment coupled with periodic vitamin A, D and E injections. But the treatments improved most of the seminal traits in the experimental group of bulls. However, benefit of treatment was not reflected in the freezability traits of the semen.

Periodic Changes of the Testis and Ductus Epididymis in Korean Hibernating Bats (韓國産 冬眠 박쥐의 精巢와 副精巢의 週期的인 變化에 관한 硏究)

  • Oh, Yung-Keun
    • The Korean Journal of Zoology
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    • v.20 no.2
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    • pp.67-76
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    • 1977
  • The periodic changes of testis and \ulcorner션 epididymidis in Korean hibernating bats, the oriental discoloured bats (Vesportilio superans Thomas) and the Korean greater horseshoe bats (Rhinolophus ferrumequinum korak Kuroda) were studied in order to clarify the possibility of correlation between their histological findings and one of physiological facets, hibernation, and the results obtained were as follows: 1. The spermatogenic function of the oriental discoloured bats obtained in July and August was depressed although the spermatocytes showed a considerable cell proliferation. Few mature sperms were observed in the seminiferous tubules of the bat obtained in August. 2. The spermatogenic function of the Korean greater horeshoe bats obtained in September was not remalkable but a considerable number of sperms were stored in the excretory ducts which were characterized by existence of para-tubular spaces in the ductus epididymidis. 3. The spermatogenic epithelia of the Korean greater horseshoe bats obtained in December showed histologically atrophied figures. However, a vast number of sperm remained in extremely expaned luminae of the ductuc epididymidis which epithelial cells were maintained rectangular in shape. 4. These results suggest that there are periodic changes of the spermatogenic epithelia and the excretory ducts, and that those histological changes are closely related to their wintering.

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