• Title/Summary/Keyword: Specimen size

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Bacterial Growth-inhibiting Activity of Amniotic Fluid Against E. coli (양수의 대장균에 대한 세균증식 억제효과)

  • Kim, Soo-Yong;Choi, Myung-Sik;Chang, Woo-Hyun;Cha, Chang-Yong
    • The Journal of the Korean Society for Microbiology
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    • v.22 no.3
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    • pp.233-240
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    • 1987
  • The amniotic fluid provides a medium in which the fetus can readily move, cushions him against possible injury and helps him maintain an even temperature. Besides above mentioned functions, investigators reported that human amniotic fluid contains host-resistance factors which prevent bacteria from producing infectious disease and this activity shows difference among human racial groups or bacterial genera, species and strains. 40 amniotic fluid specimens from Korean women in their second and third trimesters of pregnancy were examined for inhibiting the growth of Escherichia coli. And various factors which might affect bacterial growth inhibiting activity such as pH, initial inoculum size, concentration of amniotic fluid, and heat resistance, were also tested using a strongly inhibitory amniotic fluid specimen. Finally plate diffusion tests were carried out using other strongly inhibitory amniotic fluid. The following results were obtained: 1. Of the 40 fluid samples examined, 18 specimens(45%) had inhibitory activity and samples from women in their second trimester of pregnanancy showed non-inhibitory activity(2 specimens). 2. The pH of the fluids varied between 7.43 and 8.33. There was no correlation between pH and inhibitory activity. 3. No. 19 amniotic fluid showed bacteriostatic activity after 24 hours incubation when an inoculum of $10^2$ organisms per milliliter was used, but non-inhibitory with an inoculum of $10^3$ and $10^4$ bacteria per milliliter. 4. The content of amniotic fluid in culture media influenced E. coli growth. At 90 percent, E. coli was inhibited growth but at 10 percent and 50 percent. 5. Inhibitory activity of No. 19 amniotic fluid was retained after heating to $50^{\circ}C$ for 30 minutes or 100^{\circ}C$ for 30 minutes. 6. Plate diffusion tests with No. 27 amniotic fluid showed that 0.7ml amniotic fluid gave clear zone of growth inhibition around the central well but 0.2ml and 0.1ml amniotic fluids were not.

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Ex Vivo MR Diffusion Coefficient Measurement of Human Gastric Tissue (인체의 위 조직 시료에서 자기공명영상장치를 이용한 확산계수 측정에 대한 기초 연구)

  • Mun Chi-Woong;Choi, Ki-Sueng;Nana Roger;Hu, Xiaoping P.;Yang, Young-Il;Chang Hee-Kyung;Eun, Choong-Ki
    • Journal of Biomedical Engineering Research
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    • v.27 no.5
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    • pp.203-209
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    • 2006
  • The aim of this study is to investigate the feasibility of ex vivo MR diffusion tensor imaging technique in order to observe the diffusion-contrast characteristics of human gastric tissues. On normal and pathologic gastric tissues, which have been fixed in a polycarbonate plastic tube filled with 10% formalin solution, laboratory made 3D diffusion tensor Turbo FLASH pulse sequence was used to obtain high resolution MR images with voxel size of $0.5{\times}0.5{\times}0.5mm^3\;using\;64{\times}32{\times}32mm^3$ field of view in conjunction with an acquisition matrix of $128{\times}64{\times}64$. Diffusion weighted- gradient pulses were employed with b values of 0 and $600s/mm^2$ in 6 orientations. The sequence was implemented on a clinical 3.0-T MRI scanner(Siemens, Erlangen, Germany) with a home-made quadrature-typed birdcage Tx/Rx rf coil for small specimen. Diffusion tensor values in each pixel were calculated using linear algebra and singular value decomposition(SVD) algorithm. Apparent diffusion coefficient(ADC) and fractional anisotropy(FA) map were also obtained from diffusion tensor data to compare pixel intensities between normal and abnormal gastric tissues. The processing software was developed by authors using Visual C++(Microsoft, WA, U.S.A.) and mathematical/statistical library of GNUwin32(Free Software Foundation). This study shows that 3D diffusion tensor Turbo FLASH sequence is useful to resolve fine micro-structures of gastric tissue and both ADC and FA values in normal gastric tissue are higher than those in abnormal tissue. Authors expect that this study also represents another possibility of gastric carcinoma detection by visualizing diffusion characteristics of proton spins in the gastric tissues.

A Study on the Evaluation Method to Flexural-bonding Behavior of FRP-Rebar Concrete Member (FRP를 보강근으로 사용한 콘크리트 부재의 휨-부착 거동 평가방법에 관한 연구)

  • Choi, So-Yoeng;Choi, Myoung-Sung;Kim, Il-Sun;Yang, Eun-Ik
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.25 no.5
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    • pp.149-156
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    • 2021
  • FRP has been proposed to replace the steel as a reinforcement in the concrete structures for addressing the corrosion issue. However, FRP-Rebar does not behave in the same manner as steel because the properties of FRP are different. For example, FRP-Rebar has a high tensile strength, low stiffness, and linear elastic behavior which results in a difference bonding mechanism to transfer the load between the reinforcement and the surrounding concrete. Therefore, bonding behavior between FRP-Rebar and concrete has to be investigated using the bonding test. So, Pull-out test has been used to estimate bond behavior because it is simple. However, the results of pull-out test have a difference with flexural-boding behavior of FRP-Rebar concrete member. So, it is needed to evaluate the real fleuxral-bonding behavior. In this study, the evaluation method to flexural-bonding behavior of FRP-Rebar concrete member was reviewed and compared. It was found that the most accurate evaluation method for the fleuxral-bonding behavior of FRP-Rebar concrete member was splice beam test, however, the size and length of specimen have to increase than other test method and the design and analysis of splice beam is complex. Meanwhile, characteristics of concrete could be reflected by using arched beam test, unlike hinged beam test which is based on the ignored change of moment arm length in equilibrium equation. However, the possibility of shear failure exists before the flexural-bonding failure occur.

Maintenance of Platelet Counts with Low Level QC Materials and the Change in P-LCR according to Hemolysis with XN-9000 (XN-9000장비에서 Low Level QC물질에서의 혈소판 수 관리와 용혈에 따른 P-LCR의 변화)

  • Shim, Moon-Jung;Lee, Hyun-A
    • Korean Journal of Clinical Laboratory Science
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    • v.50 no.4
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    • pp.399-405
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    • 2018
  • The platelet count in clinical laboratories is essential for the diagnosis and treatment of hemostasis abnormalities, and accurate platelet counting in the low count range is of prime importance for deciding if a platelet transfusion is needed and for monitoring after chemotherapy. Quality control is designed to reduce and correct any deficiencies in the internal analytical process of a clinical laboratory prior to the release of patient results. Fragmented erythrocytes are the major confusing factors for platelet counting because of their similar size to platelets. The authors found that the low range QC values were out of 2SD with a Sysmex automatic analyzer in internal quality control process. Thus far, there has been little discussion on the relationship between hemolysis and the platelet parameters. Therefore, this study focused on the performance of automated platelet counts, including the PLT-F, the PLT-I, and PLT-O methods at the low platelet range using the low level QC materials and compared the 5 platelet parameters with the hemolyzed samples. The results showed that the CV was the smallest with PLT-F and P-LCR increased from 18.4 to 31.9% in the hemolysis samples. These results indicate that a more accurate estimation of the platelet counts can be achieved using the PLT-F method than the PLT-I method at the low platelet range. The use of the PLT-F system improves the confidence of results in low platelets samples in a routine hematology laboratory. The results suggest that P-LCR is a new parameter in assessing samples when the specimen is suspected of hemolysis and deterioration. Nevertheless, further studies will be needed to establish the relationship with P-LCR and hemolysis using human blood specimens.

The study on structural vulnerability analysis of small fixed wing UAV with hard landing (동체 착륙 방식의 소형 고정익 무인항공기 구조 취약점 분석)

  • Jeong, Seong-rok;Kang, Ju-hwan
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.7
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    • pp.20-25
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    • 2019
  • In this paper, the structural weakness analysis and quality improvement of small fixed wing UAV of the hard landing type were studied. Unlike conventional aircraft, small UAV does not use runways because of its small size. Instead, small UAV use hand launch takeoff and hard landings. This type has many operational advantages because it can take off and land in a narrow space. But, the hard landing has a strong impact on the structure of the UAV and can cause serious damage. In order to analyze the exact cause of this phenomenon, the structural analysis was carried out using the 3D structural analysis program (ABAQUS) to identify the location of the fracture. And to improve the accuracy of the structural analysis, properties of the material were obtained through specimen test. As a result of the analysis, structural weaknesses were identified and improved. Thus, the validity of the study was verified by demonstrating the quality of enhanced structure through a real impact test at a higher level of 1.5 times the maximum impact during operation.

Behavior of RC beams strengthened with NSM CFRP strips under flexural repeated loading

  • Fathuldeen, Saja Waleed;Qissab, Musab Aied
    • Structural Engineering and Mechanics
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    • v.70 no.1
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    • pp.67-80
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    • 2019
  • Strengthening with near surface mounted carbon fibre reinforced polymers (NSM-CFRP) is a strengthening technique that have been used for several decades to increase the load carrying capacity of reinforced concrete members. In Iraq, many concrete buildings and bridges were subjected to a wide range of damage as a result of the last war and many other events. Accordingly, there is a progressive increase in the strengthening of concrete structures, bridges in particular, by using CFRP strengthening techniques. Near-surface mounted carbon fibre polymer has been recently proved as a powerful strengthening technique in which the CFRP strips are sufficiently protected against external environmental conditions especially the high-temperature rates in Iraq. However, this technique has not been examined yet under repeated loading conditions such as traffic loads on bridge girders. The main objective of this research was to investigate the effectiveness of NSM-CFRP strips in reinforced concrete beams under repeated loads. Different parameters such as the number of strips, groove size, and two types of bonding materials (epoxy resin and cement-based adhesive) were considered. Fifteen NSM-CFRP strengthened beams were tested under concentrated monotonic and repeated loadings. Three beams were non-strengthened as reference specimens while the remaining were strengthened with NSM-CFRP strips and divided into three groups. Each group comprises two beams tested under monotonic loads and used as control for those tested under repeated loads in the same group. The experimental results are discussed in terms of load-deflection behavior up to failure, ductility factor, cumulative energy absorption, number of cycles to failure, and the mode of failure. The test results proved that strengthening with NSM-CFRP strips increased both the flexural strength and stiffness of the tested beams. An increase in load carrying capacity was obtained in a range of (1.47 to 4.49) times that for the non-strengthened specimens. Also, the increase in total area of CFRPs showed a slight increase in flexural capacity of (1.02) times the value of the control strengthened one tested under repeated loading. Increasing the total area of CFRP strips resulted in a reduction in ductility factor reached to (0.71) while the cumulative energy absorption increased by (1.22) times the values of the strengthened reference specimens tested under repeated loading. Moreover, the replacement of epoxy resin with cement-based adhesive as a bonding material exhibited higher ductility than specimen with epoxy resin tested under monotonic and repeated loading.

Synthesis of Reduced Graphene-metal Hybrid Materials via Ion-exchange Method and its Characterization (이온교환법에 의한 환원 그래핀-금속 하이브리드 소재의 합성 및 특성)

  • Park, Aeri;Kim, Sumin;Kim, Hyun;Han, Jong Hun
    • Journal of the Microelectronics and Packaging Society
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    • v.27 no.4
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    • pp.25-37
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    • 2020
  • In this study, hybridization of graphene oxide and metal was carried out by the functional groups containing oxygen and thermal treatment for reduction in order to enhance the electrical conductivity and magnetic properties of graphene materials. Graphene-metal hybrid materials were synthesized using the oxygen-containing functional groups (-OH, -COOH and so on) on the surface of graphene oxide by replacing them with metal ions via ion exchange method as well as thermal reduction. The metals used in this study were Fe, Ag, Ni, Zn, and Fe/Ag, and it was confirmed that metal particles of uniform size were well dispersed on the graphene surface through SEM, TEM, and EDS. All of the metal particles on the graphene surface had an oxide-crystalline structure. To check the electrical properties, sheet resistance of the rGO-metal hybrid sample was measured on the PET film made by the dip-coating, and the specific resistance was calculated by measuring the thickness of the specimen through SEM. As a result, the specific resistance was in the range of 2.14×10-5 and 3.5×10-3 ohm/cm.

Experimental Evaluation of the Flexural Behavior of SY Permanent Steel Form for RC Beam and Girder (SY 비탈형 보 거푸집의 휨 거동에 대한 실험적 고찰)

  • Bae, Kyu-Woong;Boo, Yoon-Seob;Shin, Sang-Min
    • Journal of the Korea Institute of Building Construction
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    • v.22 no.1
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    • pp.11-21
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    • 2022
  • Currently, in the domestic construction industry, the free web method has been emerging as a potential solution to the shortage of skilled workers due to the prolonged COVID-19 crisis, as it helps in securing economic feasibility through shortening the construction period and reducing labor costs. To consider one part of the construction method, in this study, the bending behavior according to the load was evaluated for the SY slope-type beam formwork, which was manufactured at a factory, assembled with rebar, brought into the site, and then poured into the site. For the SY Beam standard cross-sectional shape, a cross-sectional dimensional width of 400mm and depth 600mm determined through structural modeling using the MIDAS GEN program were applied. A total of 6 specimens were made with a member length of 5,000mm, 5 specimens and one RC specimen in the comparison group were manufactured in real-size format using the thickness of the steel plate(0.8, 1.0, 1.2mm) as a variable, and bending experiments were performed. In the bending test, the steel plate deck showed high initial stiffness and maximum strength as it yielded, which showed that it sufficiently contributed to the flexural strength. It is judged that additional analysis and experimental studies for 1.05, 1.1, and 1.15mm are needed to derive the appropriate steel plate thickness and the method for calculating the tensile force contribution of the steel plate to secure the manufacturing, construction and economic feasibility of SY Beam in the future.

Evaluation of Serviceability and Flexural Performance for RC Hollow Slab by Hollow Ratio and Damping Ratio (중공율과 감쇠율을 이용한 RC 중공 슬래브의 사용성 및 휨성능 평가)

  • Jong Hoon Kim;Dong Baek Kim;Yong Gon Kim;Jae Won Lee;Jeong Ho Choi
    • Journal of the Society of Disaster Information
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    • v.18 no.4
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    • pp.930-935
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    • 2022
  • Purpose: The purpose of this study is to evaluate the stiffness reduction and damping ratio of reinforced concrete hollow slabs and to analyze their performance, and to study the effect of the damping effect of hollow bodies and the stiffness reduction on the serviceability of slabs. Method: Test specimen was made in a size of 0.6m*0.21m*3.6m to evaluate the vibration effect of the slab, and the hollow ratio was set in six steps from 0.0% to 30% to measure the change in rigidity and damping according to the change in the hollow ratio. Result: As the hollow ratio increases, rigidity decreases and the natural frequency decreases, but as the mass decreases, the natural frequency increases gradually. Since energy is hardly dissipated up to the hollow ratio of 20%, the hollow ratio should be reduced by 30%. Conclusion: It was found that the bending strength degradation of the slab with a hollow ratio of about 30% is minimized, but an appropriate natural frequency can be maintained, and a certain damping effect can be obtained.

Possibility of Using Landfill Coal Ash as CLSM Material for Emergency Restoration of Ground and Road Joint Parts (지반 및 도로 공동부의 긴급복구용 CLSM 재료로 매립 석탄저회 활용 가능성)

  • Jin-Man Kim;Sang-Chul Shin;Kyoung-Nam Min;Ha-Seog Kim
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.11 no.1
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    • pp.55-61
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    • 2023
  • This study aims to develop CLSM fill material for emergency restoration using landfill coal ash. As a result of examining physical properties such as particle size distribution and fines content of landfill coal ash, bottom ash, fly ash, and general soil were mixed, and SP was found to have a density of 2.03 and a residual particle pass rate of 7.8 %. CLSM materials that secure fluidity in unit quantities without using chemical admixtures such as glidants and water reducing agents have a high risk of material separation due to bleeding. As a result of this experiment, it was found that the bleeding ratio did not satisfy the standard in the case of the specimen with a large amount of fly ash and a lot of addition of mixing water. As a result of the compressive strength test, the strength development of 0.5 MPa or more for 4 hours was found to be satisfactory for the specimens using hemihydrate gypsum with a unit binder amount of 200 or more, and the remaining gypsum showed poor strength development. Although it is judged that landfill coal ash can be used as a CLSM material, it is necessary to identify and apply the physical and chemical characteristics of coal ash buried in the ash treatment plant of each power generation company.