• 식물분류학회지
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• 제44권2호
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• pp.81-83
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• 2014

미기록종인 주름청사초(Carex rugata Ohwi)를 전라북도와 전라남도에서 발견하였다. 이 종은 수과 표면이 오목하게 들어가는 특징으로 인해 근연종인 청사초(C. breviculmis R. Br.), 겨사초(C. mitrata Franch.), 가지청사초(C. polyschoena H. Lev. & Vaniot), 목포사초(C. genkaiensis Ohwi), 긴목포사초(C. formosensis H. Lev. & Vaniot) 및 좀목포사초(C. kamagariensis K. Okamoto)와 쉽게 구별된다. 국명은 종소명을 고려하여 '주름청사초'로 신칭하였고, 이에 대한 기재, 도해 및 사진을 제시하였다.

• 한국응용곤충학회지
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• 제60권1호
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• pp.15-47
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• 2021

성페로몬은 곤충 종 특이적으로 교미신호를 전달하는 화학신호물질이다. 곤충의 촉각에는 이러한 성페로몬 화학물질을 받아들이는 특이적 수용체를 지닌다. 성페로몬이 이 수용체에 결합하면서 감각전위를 발생시키고 이는 대뇌로 전달되어 정보 인식을 통해 교미행동을 유발하게 한다. 성페로몬은 또한 해충의 발생을 모니터링하는 데 이용되어 온도발육모델과 더불어 향후 발생상황을 예측하는 데 널리 이용되고 있다. 더불어 성페로몬이 해충의 대량포획, 유살 또는 교미교란을 유발하여 직접적으로 방제에 응용된다. 본 종설은 성페로몬과 관련된 곤충 생리 및 이를 이용한 해충관리기술을 소개한다.

• Journal of Microbiology
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• 제41권3호
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• pp.252-258
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• 2003

The carbon utilizations of Bacillus species and Pythium species were investigated by using a Biolog$^{(R)}$ microplate assay to determine if there are differences in the carbon utilizations of selected strains of these species. It may be possible to afford a competitive advantage to bacterial biological control agents by providing them with a substrate that they can readily use as a carbon source, for example, in a seed coating formulation. Microplates, identified as SFP, SFN and YT were used to identify spore-forming bacteria, nonspore-forming bacteria, and yeast, respectively. Bacterial and mycelial suspensions were adjusted to turbidities of 0.10 to 0.11 at 600 nm. One hundred microliters of each of the bacterial and mycelial suspension were inoculated into each well of each of the three types of microplates. L-arabinose, D-galactose, D-melezitose and D-melibiose of the 147 carbohydrates tested were found to be utilized only by bacteria, and not by Pythium species, by Biolog$^{(R)}$ microplate assay, and this was confirmed by traditional shake flask culture. Thus, it indicated that the Biolog$^{(R)}$ microplate assay could be readily used to search for specific carbon sources that could be utilized to increase the abilities of bacterial biological control agents to adapt to contrived environments.

• Journal of Ecology and Environment
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• 제40권1호
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• pp.45-54
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• 2016

Background: Macrophytes are commonly utilised as habitat by epiphytic species; thus, complex macrophyte structures can support high diversities and abundances of epiphytic species. We tested the hypothesis that the presence of aquatic macrophytes is an important factor determining Sida crystallina (O.F. Muller, 1776) distribution. Results: An ecological survey was conducted in 147 lentic freshwater bodies. S. crystallina was frequently observed, and its density was strongly associated with macrophyte abundance. S. crystallina was found on emergent plant species such as Phragmites australis and Paspalum distichum, attached to the stem surfaces by adhesive substances secreted by the nuchal organ. Thus, S. crystallina was more strongly attached to macrophytes than to other epiphytic cladoceran species. We found higher densities of S. crystallina in filtered water with increased macrophyte shaking effort (i.e. 10, 20, 40, or 80 times). S. crystallina attachment was not related to fish predation. Stable isotope analysis showed that S. crystallina utilises epiphytic organic matter (EOM) on macrophytes as a food source. Conclusions: Consequently, S. crystallina seems to have a strong association with species-specific macrophyte biomass than with other cladoceran species, which may contribute to this species' predominance in various freshwater ecosystems where macrophytes are abundant.

• 한국약용작물학회지
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• 제18권6호
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• pp.379-388
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• 2010

Adenophora racemosa is recently reported as a new Korean endemic plant species. However, the phylogenetic relationship of this genus has been controversial due to the morphological similarity and frequent morphological change of aerial parts. To verify the phylogenetic position of Adenophora racemosa and phylogenetic relationship of genus Adenophora, we analyzed the internal transcribed spacer (ITS) sequence of nuclear ribosomal DNA (nrDNA) and random amplified polymorphic DNA (RAPD) using 21 individual of 6 Adenophora species, A. verticillata, A. divaricata, A. racemosa, A. remotiflora, A. stricata and A. tetraphylla. In comparative analysis of the nrDNA-ITS sequences, we could not found not only any species specific nucleotide sequence but also could not estimated their inter or intra species. In the phylogenic analysis based on the RAPD derived DNA polymorphism, Adenophora species were classified into four groups by clustering analysis of the UPGMA. These results suggest that the DNA fingerprinting based on RAPD is more suitable than nrDNA-ITS sequence for the phylogenetic analysis of Adenophora species.

• 한국환경과학회지
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• 제20권4호
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• pp.443-455
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• 2011

This study was conducted to investigate the flora of Mt. Jang located at Haeundae-Gu in Busan. The survey for the flora was carried out from Oct. 2008 to Sep. 2009. The results were obtained as follows. The flora consists of 5 forms, 22 varieties, 238 species, 198 genera, 91 families, 47 orders, 7 classes, and 3 divisions as total 265 taxa. The typical communities were Osmunda japonica com., Stephanandra incisa com., Gleichenia japonica com., Eurya japonica-Pinus thunbergii com., Styrax japonica-Pinus thunbergii com., and Smilax china com. which were distributed along Forest Bathing Area and Yangun Falls, Miscanthus sinensis for. purpurascens com., Carpinus turczaibovii var. coreana com and Pseudosasa japonica were distributed around the top of mountain. According to the ecological characteristic species, the evergreen plants were observed 15 taxa 14 genera 13 families, the naturalized plants 18 taxa 17 genera 10 families, the specific plants categorized by Ministry of Environment 20 taxa 18 genera 18 families, the endangered plant 1 specie Utricularia yakusimensis, and the cultivated plants were observed 21 taxa 18 genera 14 families.

• The Plant Pathology Journal
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• 제30권1호
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• pp.68-74
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• 2014

Chrysanthemum stunt viroid (CSVd), a noncoding infectious RNA molecule, causes seriously economic losses of chrysanthemum for 3 or 4 years after its first infection. Monomeric cDNA clones of CSVd isolate SK1 (CSVd-SK1) were constructed in the plasmids pGEM-T easy vector and pUC19 vector. Linear positive-sense transcripts synthesized in vitro from the full-length monomeric cDNA clones of CSVd-SK1 could infect systemically tomato seedlings and chrysanthemum plants, suggesting that the linear CSVd RNA transcribed from the cDNA clones could be replicated as efficiently as circular CSVd in host species. However, direct inoculation of plasmid cDNA clones containing full-length monomeric cDNA of CSVd-SK1 failed to infect tomato and chrysanthemum and linear negative-sense transcripts from the plasmid DNAs were not infectious in the two plant species. The cDNA sequences of progeny viroid in systemically infected tomato and chrysanthemum showed a few substitutions at a specific nucleotide position, but there were no deletions and insertions in the sequences of the CSVd progeny from tomato and chrysanthemum plants.

• The Plant Pathology Journal
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• 제38권4호
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• pp.296-303
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• 2022

The cucurbit powdery mildew (CPM) caused by different fungal species is a major concern for cucurbit crops around the world. In Argentina CPM constitutes the most common and damaging disease for cucurbits, especially for squash crops (Cucurbita moschata). The present study displays initial insights into the knowledge of the disease in western Argentina, including the determination of the prevalent species causing CPM, as well as the evaluation of the resistance of squash cultivars and breeding lines. Fungal colonies were isolated from samples collected in Mendoza province, Argentina. A field trial was also performed to assess the resistance of five squash accessions, including commercial cultivars and breeding lines. The severity of CPM was analyzed and epidemiological models were built based on empirical data. The morphological determinations and analysis with specific molecular markers confirmed Podosphaera xanthi as the prevalent causal agent of CPM in Mendoza. The results od the field trial showed differences in the resistance trait among the squash accessions. The advanced breeding line BL717/1 showed promising results as source of CPM resistance for the future development of open pollinated resistant cultivars, a crucial tool for an integrative control of the disease.

• Journal of Plant Biotechnology
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• 제48권1호
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• pp.18-25
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• 2021

멕시코로부터 유래한 Solanum demissum은 감자 야생종 중의하나로 감자 역병에 대해 저항성을 가지고 있어 감자 육종에서 중요한 재료로 이용되고 있다. S. demissum의 EBN은 4배 체인 감자와 같은 4로 직접적인 교배로 육종에 활용될 수 있다. 본 연구에서는 NGS 기술에 의해 완성된 S. demissum의 엽록체 전장 유전체(cpDNA)와 이를 다른 Solanum종과의 비교를 통해 개발한 분자마커에 대해 보고하였다. S. demissum의 전체 cpDNA의 크기는 155,558 bp였으며 그 구조는 다른 Solanum종과 매우 유사하였다. S. demissum의 cpDNA와 가지과에 속하는 10개 종의 cpDNA 코딩서열을 이용하여 분석한 계통수에서는 S. demissum이 S. hougasii 및 S. stoloniferum과 거의 동일한 유전체 구성을 보였으며, 다음으로 S. berthaultii 및 S. tuberosum과 유연관계가 가까운 것으로 확인되었다. S. demissum과 다른 7종의 Solanum과의 전체 cpDNA 다중 정렬을 통해 S. demissum 특이적인 두 개의 InDel 영역을 구명하였으며 이를 기반으로 최종적으로 PCR을 기반으로 한 두 개의 S. demissum 특이적 마커를 개발하였다. 본 연구의 결과는 Solanum 종들을 대상으로 한 조금 더 세부적인 진화적 그리고 육종적 측면에서의 연구에 기여를 할 수 있을 것이다.

• Journal of Plant Biotechnology
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• 제31권1호
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• pp.1-6
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• 2004

한약재로 사용되는 방풍류는 절단되어 유통되므로 외부 형태적인 특징만으로 구분하기가 어려워 방풍류로 사용되는 방풍, 식방풍, 석방풍, 갯방풍 등 4종에 대해 PCR에 기초한 RAPD 마커를 이용하여 SCAR 마커를 개발하고자 하였다. RAPD 분석결과 밴드의 패턴은 다양하게 나타났으며 다형성의 밴드 수는 총 215개로 전체 밴드수의 98%였다. RAPD 분석에서 각 방풍류를 구별 할 수 있는 특이적인 밴드를 나타내는 primer는 방풍에서 4개의 primer, 식방풍은 6개의 primer, 석방풍은 4개의 primer, 갯방풍은 6개의 primer를 선발하였고, 그 중 특히 primer 425는 4종의 방풍류의 감별에 유용하였고, 이를 이용하여 SCAR마커로 전환하는데 이용하였다. 특이적인 단편을 클로닝하여 염기서열 분석으로 특이 primer를 제작하고 제작된 primer로 방풍류 시료 16개에 적용하였을 때, 국내의 야생에서 주로 자생하는 석방풍은 215 bp, 그리고 국내에서 가장 많이 재배 또는 생산되는 갯방풍은 177 bp와 300 bp에서 뚜렷하게 나타났다. 따라서 갯방풍과 석방풍의 감별 가능성을 제시할 수 있으며 개발된 SCAR 마커를 이용하여 시중에 유통되고 있는 방풍류 건조약재의 감별에 유용한 마커로 활용될 수 있을 것이다.