• The Plant Pathology Journal
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• v.15 no.5
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• pp.287-294
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• 1999

A technique based on the polymerase chain reaction (PCR) for the specific detection of genus Phytophthora and Phytophthora cryptogea-P. drechsleri complex group was developed using nucleotide sequence information of ribosomal DNA (rDNA) regions. The internal transcribed spacers (ITS) including 5.8S were sequenced for P. cryptogea-P. drechsleri complex group and its related species. Two pairs of oligonucleotide primers were designed. Primer pair ITS1/Phy amplified ca. 240 bp fragment in 12 out of 13 specie of Phytophthora, but not in Pythium spp., Fusarium spp.and Rhizoctonia solani. Primer pair rPhy/Pcd amplified 549 bp fragment only in P. cryptogea-P. drechsleri complex group, but not in other Phytophthora spp.and other genera. Specific PCR amplification using the primers was successful in detecting Phytophthora and P. cryptogea-P. drechsleri complex group in diseased plants.

• The Plant Pathology Journal
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• v.18 no.1
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• pp.12-17
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• 2002

For the molecular detection of Sweet potaio feathery mottle virus (SPFMV) from diseased sweet potato plants, reverse transcription and polymerase chain reaction (RT-PCR) was performed with the use of a set of virus-specific primers to amplify an 816 bp product. The viral coat protein gene was selected for the design of the primers. No PCR product was amplified when Turnip mosaic virus, Potato vims Y or Cucumber mosaic virus were used as template in RT-PCR with the SPFMV-specific primers. The lowest concentration of template viral RNA required for detection was 10 fg. The vim was rapidly detected from total nucleic acids of leaves and roots from the virus-infected sweet potato plants as well as from the purified viral RNA by the RT-PCR. Twenty-four sweet potato samples were selected and analyzed by RT-PCR and restriction fragment length polymorphism (RFLP). RFLP analysis of the PCR products showed three restriction patterns, which resulted in some point mutations suggesting the existence of quasi-species for the vims in the infected sweet potato plants.

• Korean Journal of Plant Resources
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• v.29 no.2
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• pp.264-280
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• 2016

This survey was conducted in order to investigate the vascular plants of Mt. Goryeong and Gaemyeong in the DMZ area of Korea. The vascular plants were collected eight times from April 2014 to October 2014 and consisted a total of 450 taxa; 91 families, 279 genera, 391 species, 4 subspecies, 49 varieties and 6 forms. The observed rare plant species as designated by Korea Forest Service were 5 taxa including Tylophora floribunda Miq., Aristolochia contorta Bunge, Viola albida Palib., etc. Endemic plant species were 6 taxa containing Aconitum pseudolaeve Nakai, Clematis brachyura Maxim., Clematis trichotoma Nakai, etc. 33 taxa were listed as specific plant species based on phytogeographical in the investigated area. 35 taxa were recorded as naturalized plants, and their naturalized rate and urban index were noted as 7.7%, and 10.9%, respectively. Of the 450 taxa listed; 186 were edible plants, 173 pasture plants, 149 medicinal plants, 51 omamental plants, 20 timber plants, 13 stain plants, 4 fiber plants, 4 industrial plants and 92 unknown resource plants.

• Korean Journal of Plant Resources
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• v.27 no.4
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• pp.293-308
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• 2014

This study was carried out to investigate the vascular plants in the construction site of the National DMZ Native Botanic Garden. The period of survey was from May 2012 to November 2013. Vascular plants based on voucher specimen were summarized as 313 taxa including 79 families, 211 genera, 272 species, 4 subspecies, 32 varieties, 4 forms and 1 hybrids. The rare plant species designated by Korea Forest Service were 8 taxa including Galium boreale L., Eleutherococcus senticosus (Rupr. & Maxim.) Maxim., Eranthis stellata Maxim. and Lloydia triflora (Ledeb.) Baker, etc. Endemic plant species were 4 taxa including Salix koriyanagi Kimura, Clematis trichotoma Nakai, Philadelphus schrenkii Rupr. and Cirsium setidens (Dunn) Nakai. Furthermore, 51 taxa were listed as specific plant species based on phytogeographical in the investigated area. The naturalized plants were recorded as 11 taxa, and their Naturalization Ratio and Urbanization Index were recorded as 3.51%, and 3.43%, respectively.

• Korean Journal of Medicinal Crop Science
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• v.8 no.3
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• pp.243-249
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• 2000

Analysis of random amplified polymorphic DNAs(RAPDs) and internal morphological features were performed using three species of medicinal plants in the genus of Angelica(A. gigas Nakai, A. sinensis(Oliv.) Diels., A. acutiloba Kitagawa) to distinguish between these three species. Fifty decarmer oligonucleotide primers were screened for the RAPDs of the herbal plant species. Five primers generated distinct RAPD markers specific to the species of Angelica, In analysis of the degree of similarity, A. sinensis(Oliv.) Diels is more closely related to A. acutiloba Kitagawa than to A. gigas Nakai. Furthermore, we proved the usefulness of RAPD analysis for the discrimination of the species using dry roots and commercial plant materials. In internal morphology of three species, A. sinensis(Oliv.) Diels seemed to be more specialized in systemic than A. acutiloba Kitagawa and A. gigas Nakai

• Korean Journal of Plant Resources
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• v.9 no.3
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• pp.298-304
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• 1996

The fruit wall anatomy of Ocotea was investigated on the basis of 14 species within the genus to contribute to a better understanding of specific relationships and homogeneity of genus. The species have a similar mature fruit wall structure, but diUerences among the species are found with respect to whether or not sdc.nchyma cells are present in the mesocarp. if present, whether or not they are present in particular positions and forms. Comparisons with species studied suggested that at least a few groups of species can be distinguished in Ocotea. They arc divided into five groups on the basis of anatomical structures. i.e., group 1) O. atrriensis, O. cujumari, O. helicterifolia, O. rubra and O. schomburgkiana; group 2) O. aeiphylla, O. javitensis, and O. sp. [Werff et ai. 12676]; group 3) O. tonduzii: group 4) O. foetens, O. quixos, and O. veraguensis; and group 5) O. floribunda and O. nitida. These various variations in Ocntea were also discussed to invite its respective systematic revisions. By the comparisons with species, on the other hand, it suggested that the specialized species are evolved from non-specialized species.

• Advances in Traditional Medicine
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• v.7 no.1
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• pp.26-33
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• 2007

Genetic polymorphism and molecular authentication were investigated with the commercial medicinal herb, Peony (Paeonia spp.), using random amplified polymorphic DNA (RAPD) markers. To identify the polymorphism of the RAPD patterns among plant origins, 20 different random primers were applied to the genomic DNA extracted from Paeonia spp. plants such as Paeonia (P.) lactiflora, P. officinale and P. japonica. Ten primers out of 20 primers could be used to discriminate the plant species in the same genus and 72 out of 81 scored DNA fragments (88.9%) generated with these primers were polymorphic. Especially, four primers, such as OPA1, OPA3, OP9, and OPA13, were useful to discriminate the plant origins among the species of Peony. In the results of cluster analysis using RAPD data obtained from the 10 primers, Peony (Paeonia spp.) plants used in this study were grouped into the two distinctive clusters, genetically. Herb medicine, especially P. lactiflora, were easily identified, when species-specific primers were applied to the investigation for discriminating herb medicine currently traded in domestic herb market, Kyungdongmart. Consequently, RAPD analysis was useful method to discriminate plant origins and the commercial medicinal herbs, Paeonia spp..

• Korean Journal of Plant Resources
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• v.31 no.5
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• pp.575-589
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• 2018

The purpose of this study is to provide the baseline data for conservation and management of the ecosystem of Gyeongju National Park by surveying and analysing the vascular plants distributed in Mt. Manhongbong (522 m). The vascular plants were surveyed form May 2012 to August 2017. The vascular plants on this site was classified as follow. Based on voucher specimens, the vascular plants of this site consisted of 91 families, 292 genera, 397 species, 4 subspecies, 48 varieties and 8 forms. The 2 taxa of threatened species, 11 taxa of rare plants and 14 taxa of Korean endemic plants were investigated. The specific plants by floristic region were totally 39 taxa, which were 3 taxa of grade V, 5 taxa of grade IV, 2 taxa of grade III, 11 taxa of grade II and 18 taxa of grade I. The naturalized plants were identified as 33 taxa and the percentage of naturalized index (NI) was 7.2%, and urbanized index (UI) was 10.3%, respectively. The invasive alien plants were 2 taxa, such as Ambrosia artemisiifolia L. and Aster pilosus Willd.

• The Plant Pathology Journal
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• v.32 no.6
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• pp.500-507
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• 2016

Single-molecule real-time (SMRT) sequencing allows identification of methylated DNA bases and methylation patterns/motifs at the genome level. Using SMRT sequencing, diverse bacterial methylomes including those of Helicobacter pylori, Lactobacillus spp., and Escherichia coli have been determined, and previously unreported DNA methylation motifs have been identified. However, the methylomes of Xanthomonas species, which belong to the most important plant pathogenic bacterial genus, have not been documented. Here, we report the methylomes of Xanthomonas axonopodis pv. glycines (Xag) strain 8ra and X. campestris pv. vesicatoria (Xcv) strain 85-10. We identified $N^6$-methyladenine (6mA) and $N^4$-methylcytosine (4mC) modification in both genomes. In addition, we assigned putative DNA methylation motifs including previously unreported methylation motifs via REBASE and MotifMaker, and compared methylation patterns in both species. Although Xag and Xcv belong to the same genus, their methylation patterns were dramatically different. The number of 4mC DNA bases in Xag (66,682) was significantly higher (29 fold) than in Xcv (2,321). In contrast, the number of 6mA DNA bases (4,147) in Xag was comparable to the number in Xcv (5,491). Strikingly, there were no common or shared motifs in the 10 most frequently methylated motifs of both strains, indicating they possess unique species- or strain-specific methylation motifs. Among the 20 most frequent motifs from both strains, for 9 motifs at least 1% of the methylated bases were located in putative promoter regions. Methylome analysis by SMRT sequencing technology is the first step toward understanding the biology and functions of DNA methylation in this genus.

• Journal of Microbiology and Biotechnology
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• v.20 no.1
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• pp.193-201
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• 2010

A specific and rapid real-time PCR assay for detecting Ralstonia solanacearum in horticultural soil and plant tissues was developed in this study. The specific primers RSF/RSR were designed based on the upstream region of the UDP-3-O-acyl-GlcNAc deacetylase gene from R. solanacearum, and a PCR product of 159 bp was amplified specifically from 28 strains of R. solanacearum, which represent all genetically diverse AluI types and all 6 biovars, but not from any other nontarget species. The detection limit of $10^2\;CFU/g$ tomato stem and horticultural soil was achieved in this real-time PCR assay. The high sensitivity and specificity observed with field samples as well as with artificially infected samples suggested that this method might be a useful tool for detection and quantification of R. solanacearum in precise forecast and diagnosis.