• Korean Journal of Environment and Ecology
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• v.16 no.1
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• pp.68-86
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• 2002

The vascular plants in Bulgapsan, Younggwang-gun, Jeollanam-do, were consisted of 437 taxi; 104 families, 289 genus, 382 species, 51 varieties, 4 forma. From the floristic point of view, Bulgapsan is belongs to the South Province in Korea represented as Platycarya strobilacea, Cudrania tricuspidata, Mallotus japonicus, Lindera glauca, Euscaphis japonica, Meliosma myriantha, Euryu japonica, Halorrhagis micrantha, Hydeocotyle maritima, H. ramifloru, Chionanthus retusa, Trachelospermum asiaticum var. intermedium and Cymbidium goeringii, The Specific plants which is categorized to the Degree by the Ministry of Environment appeared to 25 taxa; Degree V was 2 species, Degree IV was 15 species, Degree III was 7 species and Degree II was 1 species. The mark evaluated by Degrees of five evaluation unit was 186.

• International Journal of Oral Biology
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• v.31 no.1
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• pp.11-14
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• 2006

This study was undertaken to develop PCR primers for the identification and detection of Streptococcus anginosus using species-specific forward and reverse primers. These primers targeted the variable regions of the 16S ribosomal RNA coding gene(rDNA). The primer specificity was tested against 12 S. anginosus strains and 6 different species(10 strains) of oral bacteria. The primer sensitivity was determined by testing serial dilutions of the purified genomic DNA of S. anginosus ATCC $33397^T$. The data showed that species-specific amplicons were obtained from all the S. anginosus strains tested, but not in the six other species. The PCR could detect as little as 0.4pg of the chromosomal DNA from S. anginosus. This suggests that the PCR primers are highly sensitive and applicable to the detection and identification of S. anginosus.

• Preventive Nutrition and Food Science
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• v.15 no.2
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• pp.159-166
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• 2010

The aim of this study was to develop species-specific primer sets for kimchi Lactobacillus. Known gene sequences of Lactobacillus 16S rRNA were collected from the NCBI Gene bank, and 69 primer sets were designed using the homologous gene sequence. Six species of kimchi Lactobacilli were used as reference strains: Lactobacillus brevis KCTC3102, Lactobacillus farciminis KCTC3681, Lactobacillus fermentum KCTC3112, Lactobacillus hilgardii KCTC3500, Lactobacillus plantarum KCTC3099, and Lactobacillus sanfranciscensis KCTC3205. PCR amplification and gel electrophoresis were performed to identify the accuracy and specificity of the developed primer set. The results show that the primer set of 5'-aagcctgcgaaggcaag-3' & 5'-aggccaccggctttg-3', 5'-acatactatgcaaatctaagagattagacg-3' & 5'-actgagaatggctttaagagattagcttac-3' resulted in a specific PCR band on L. hilgardii, and primer set of 5'-ctaataccgcataacaactactttcacat-3' & 5'-aacttaataaaccgcctacattctctttac-3' on L. farciminis. The results indicate that the developed primer sets can provide a useful tool for the identification and differentiation of L. hilgardii and L. farciminis from other Lactobacillus species of kimchi.

• Journal of Microbiology
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• v.43 no.2
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• pp.209-212
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• 2005

The purpose of this study was to develop species-specific PCR primers for use in the identification and detection of Actinobacillus actinomycetemcomitans. These primers target variable regions of the 168 ribosomal RNA coding gene (rDNA). We assessed the specificity of the primers against 9 A. actinomycetemcomitans strains and 11 strains (3 species) of the Haemophilus genus. Primer sensitivity was determined by testing serial dilutions of the purified genomic DNAs of A. actinomycetemcomitans ATCC$ 33384^$T Our obtained data revealed that we had obtained species-specific amplicons for all of the tested A. actinomycetemcomitans strains, and that none of these amplicons occurred in any of the other species. Our PCR protocol proved able to detect as little as 4 fg of A. actinomycetemcomitans chromosomal DNA. Our findings suggest that these PCR primers are incredibly sensitive, and should prove suitable for application in epidemiological studies, as well as the diagnosis and monitoring of periodontal pathogens after treatment for periodontitis.

• Fisheries and Aquatic Sciences
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• v.21 no.3
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• pp.8.1-8.6
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• 2018

Desmarestia japonica (Phaeophyceae, Desmarestiales) was recently established from the Japanese ligulate Desmarestia and is morphologically similar to D. ligulata. This species has been reported only from Japan. However, the taxonomic reports based on additional regional distributions are needed to clarify this taxonomic entity and its species boundaries. Because Desmarestia species have restricted distributions in Korea, we reexamined herbarium specimens of D. ligulata deposited at the National Institute of Biological Resources (South Korea). To improve the amplification efficiency of the polymerase chain reaction and avoid contamination by the DNA of other organisms, we developed taxon-specific molecular markers suitable for DNA barcoding of Desmarestia species. Nuclear ribosomal small subunit RNA (18S rDNA) and mitochondrial cytochrome c oxidase 1 (cox1) regions were selected as target DNA. As a result, both were successfully isolated from herbarium specimens of D. japonica acquired over 10 years. These molecular markers provide useful genetic information for herbarium specimens for which conventional molecular analysis is challenging.

• Korean Journal of Breeding Science
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• v.43 no.4
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• pp.265-272
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• 2011

This study is to generate SCARs markers for identification of Perilla species. A SCAR is a genomic DNA fragment at a single genetically defined locus that is identified by PCR amplification using a pair of specific oligonucleotide primers. We derived SCARs by sequencing and cloning the both ends of the amplified products of RAPD markers. Sixteen sequence-specific primers were synthesized from eight RAPD markers, which were completely sequenced. We developed the species-specific SCAR markers which could be used successfully in detecting genetic variation in four Perilla species. These markers could be used to verify species-origins of various forms of Perilla germplasms.

• Genomics & Informatics
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• v.16 no.4
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• pp.28.1-28.6
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• 2018

Due to the increasing interest in synonymous codons, several codon bias-related terms were introduced. As one measure of them, the tRNA adaptation index (tAI) was invented about a decade ago. The tAI is a measure of translational efficiency for a gene and is calculated based on the abundance of intracellular tRNA and the binding strength between a codon and a tRNA. The index has been widely used in various fields of molecular evolution, genetics, and pharmacology. Afterwards, an improved version of the index, named specific tRNA adaptation index (stAI), was developed by adapting tRNA copy numbers in species. Although a subsequently developed webserver (stAIcalc) provided tools that calculated stAI values, it was not available to access pre-calculated values. In addition to about 100 species in stAIcalc, we calculated stAI values for whole coding sequences in 148 species. To enable easy access to this index, we constructed a novel web database, named STADIUM (Species-specific tRNA adaptive index compendium). STADIUM provides not only the stAI value of each gene but also statistics based on pathway-based classification. The database is expected to help researchers who have interests in codon optimality and the role of synonymous codons. STADIUM is freely available at http://stadium.pmrc.re.kr.

• Journal of the Korea Furniture Society
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• v.10 no.1
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• pp.57-63
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• 1999

This study was carried out to analyze the relationship between basic physical properties and acoustical characteristics of imported violin wood and to offer the information on raw material procurement and incoming-material quality control to domestic violin makers which have purchased most raw materials from European exporting countries at high expense. Equilibrium moisture content of European spruce with lower specific gravity after the prolonged storage was rather higher than that of European maple with higher specific gravity. The specific gravity of spruce increased with decreasing annual ring width, however, that of narrower annual ring. Increasement in specific gravity enhanced the dynamic Young's modulus of both wood species, but influenced the vibration energy loss by internal friction differently between two species. For dynamic MOE, qurater-sawn spruce was higher than the flat-sawn, but maple showed the reverse directional characteristics. Consequently, it would be well for violin makers to establish the standard for materials and quality control system to assure the quality of their violin products.

• Journal of the Korea Furniture Society
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• v.11 no.2
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• pp.25-35
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• 2000

This study was carried out to investigate the wood properties for development the proper uses of lesser-known tree species. The tested species are Tamiang(Hydnocarpus woodii) Bangelem (Strombosia zeylanica) and Kamasulan(Anisophyllea ferruginea) grown in Indonesia. fundamental wood properties such as anatomical, physical and chemical properties and characteristics related to wood processing were examined. The results are summarized as follows : These species are diffuse porous, interlocked grained and coarse textured wood. Exceptionally, texture of Tamiang is fine. The heartwoods of these species are not distinguished clearly to the sapwoods. These species have high specific gravity, high shrinkage, and excellent strength properties. However, Tamiang has moderate specific gravity. Sawing characteristics are poor for these species except Tamiang. Sawing characteristics is moderate for Tamiang. Drying characteristics are moderate for Tamiang and Bangelem, and Poor for Kamasulan. The surfacing characteristics by planer are moderate f3r all these species. Bending property and durability are poor. Ash content is low in Bangelem.

• 한국균학회소식:학술대회논문집
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• 2018.05a
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• pp.35-35
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• 2018

Arbuscular mycorrhizal fungi (AMF) are one of the most widespread symbionts globally. Owing to their enhanced nutrient absorption capacity, AMF significantly contribute to the survival of individual plants and the ecosystem functioning. Community structures of AMF are affected by many environmental factors Inland wetlands have a different environment from common forest soils, therefore, plants inhabiting wetlands may have characteristic AMF communities. The purpose of this study was to compare the AMF communities in wetlands, among the species of host plants. We sampled the roots of 3 host plant species, Phragmites communis, Miscanthus sacchariflorus, and Trisetum bifidum with rhizospheres from 3 isolated areas in Upo wetland, Korea. We extracted DNA from AMF spores in rhizospheres and the roots of 3 plant species. We amplified 18S rDNA of AMF using AMF specific primer. As a result, we confirmed 9 species from 5 genera in AMF spores, and 5 species from 3 genera in plant roots. Funneliformis caledonium was the most dominant species in field soils, on the other hand, Diversispora aurantia was the most dominant species in plant roots. We confirmed that species diversity and abundance of AMF communities were different among host plant species. These results showed that the AMF community had specific to host plants in the inland wetland.