International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
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Identification and Detection of Streptococcus anginosus Using Species-Specific 16S rDNA Primers

  • Cho, Ji-Sun (Department of Conservative Dentistry, College of Dentistry, Chosun University) ;
  • Yoo, So-Young (Department of Oral Biochemistry, College of Dentistry, Chosun University) ;
  • Kim, Hwa-Sook (Department of Oral Biochemistry, College of Dentistry, Chosun University) ;
  • Hwang, Ho-Keel (Department of Conservative Dentistry, College of Dentistry, Chosun University) ;
  • Min, Jeong-Beom (Department of Conservative Dentistry, College of Dentistry, Chosun University) ;
  • Kim, Byung-Hoon (Engineering Research Institute, Chonnam National University) ;
  • Baek, Dong-Heon (Department of Oral Microbiology and Immunology, College of Dentistry, Dankook University) ;
  • Shin, Hwan-Seon (Department of Oral Biochemistry, College of Dentistry, Chosun University) ;
  • Kook, Joong-Ki (Department of Oral Biochemistry, College of Dentistry, Chosun University)
  • Published : 2006.03.30
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Abstract

This study was undertaken to develop PCR primers for the identification and detection of Streptococcus anginosus using species-specific forward and reverse primers. These primers targeted the variable regions of the 16S ribosomal RNA coding gene(rDNA). The primer specificity was tested against 12 S. anginosus strains and 6 different species(10 strains) of oral bacteria. The primer sensitivity was determined by testing serial dilutions of the purified genomic DNA of S. anginosus ATCC $33397^T$. The data showed that species-specific amplicons were obtained from all the S. anginosus strains tested, but not in the six other species. The PCR could detect as little as 0.4pg of the chromosomal DNA from S. anginosus. This suggests that the PCR primers are highly sensitive and applicable to the detection and identification of S. anginosus.

Keywords

References

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