• Special Issue of the Society of Naval Architects of Korea
• /
• 2007.09a
• /
• pp.89-93
• /
• 2007

The Boil-off gas (BOG) generation during the voyage is inevitable since Natural Gas (NG) in normally liquefied below -160 degree C in atmosphere condition and small heat ingress due to relatively hot outside keeps evaporating continuously. The one of major issue in LNG carriers is to handle generated BOG from cargo tank. The generated BOG affects to increase the cargo tank pressure and Gas Management System (GMS) for LNG carriers is closely related to cargo tank pressure maintenance. Economically, BOG is generally used as fuel in LNG carrier. Newly developed GMS for LNG carrier in boiler propulsion system, VaCo System, not only accomplish automatic control in GMS but also ensure safer operation.

• Plant Resources
• /
• v.5 no.1
• /
• pp.74-77
• /
• 2002

This study was performed to investigate the effect of Leonuri Herba extract on the treatment of chromaticity and colorfastness. Leonuri Herba has been used as a Korean medicine for the treatment of woman disease. In the long history of Korea, dyeing has been applied for a means representing the grace of natural and inner esthetic consciousness of man. Vegetable dyes give us such great benefits, diversified color, but no pollution. And ramie fabric has distinctive features such as beautiful brilliance, elegance, and strong durability. So, it is regarded as a special product of Korea traditionally. These studies were carried out to treat with acetate iron, dichloride copper and alum with a mordant to ramie fabric. The ramie fabric was died with Leonuri Herba extract. The results of experiment showed as follows: First, the chromophoric degree was the highest in acetate iron but not distinction in another mordants. Seocond, the light colorfastness was the highest in non treated and dichloride copper, but alum was the lowest. Third, the discoloration was alum and dichloride copper showed first grade in washing colorfastness. Abrasion colorfastness was not significant in this test. According the previous results, Leonuri Herba has an efficiency on woman disease. So it is considered that Leonuri Herba can be applied effectively to a theraphy of climacteric disturbance.

• Proceedings of the Botanical Society of Korea Conference
• /
• 1985.08b
• /
• pp.35-48
• /
• 1985

Plastids, which are organelles unique to plant cells, bear their own genome that is organized into DNA-protein complexes (nucleoids). Regulation of gene expression in the plastid has been extensively investigated because this organelle plays an important role in photosynthesis. Few attempts, however, have been made to characterize the regulation of plastid gene expression at the chromosomal structure, using plastid nucleoids. In this report, we summarize the recent progress in the characterization of DNA-binding proteins in plastids, with special emphasis on CND41, a DNA binding protein, which we recently identified in the choloroplast nucleoids from photomixotrophically cultured tobacco cells. CND41 is a protein of 502 amino acids which consisted of a transit peptide of 120 amino acids and a mature protein of 382 amino acids. The N-terminal of the 'mature' protein has lysine-rich region which is essential for DNA-binding. CNA41 also showed significant identities to some aspartyl proteases. Protease activity of purified CND41 has been recently confirmed and characterized. On the other hand, characterization of accumulation of CND41 both in wild type and transgenic tobacco with reduced amount of CND41 suggests that CND41 is a negative regulator in chloroplast gene expression. Further investigation indicated that gene expression of CND41 is cell-specifically and developmentally regulated as well as sugar-induced expression. The reduction of CND41 expression in transgenic tobacco also brought the stunted plant growth due to the reduced cell length in stem. GA3 treatment on apical meristem reversed the dwarf phenotype in the transformants. Effects of CND41 expression on GA biosynthesis will be discussed

• Proceedings of the Ginseng society Conference
• /
• 1998.06a
• /
• pp.90-95
• /
• 1998

Symbiotic associations between land plants and fungi have been known for more than one hundred years. Vesiculararbuscular mycorrhizas (VAM) are the most common symbiosis in flowering plants and can be recognized in almost all plant families. These fungal associations play a very important role in the growth and survival of plant species. However, with respect to the importance and intensity of the VAM, there is great variation among host species. Our knowledge of the VAM fungus-plant association in Araliaceae is very limited. After the first reports of the occurence of VAM in lateral roots of Panax species, mycorrhizal structures are now described as special structures representing the so-called Paris type. In this type, the development of new spores and vesicles is extremely low. This and the type of colonization of the fungus in Panax roots indicates on, one hand, the high intensity of the VAM and, on the other hand, a remarkable dependency for VAM in members of the Panax species. Therefore, it can be easily understood that cultivated Panax plants exhibit a significant uptake of nutrients and this leads to an extremely depleted soil at harvest. Further, the soil is nearly free of the spores of VAM fungi as they germinate each year on the newly developing Panax roots.

• Korean journal of applied entomology
• /
• v.53 no.3
• /
• pp.317-319
• /
• 2014

The aloe vera aphid, Aloephagus myersi Essig, was collected on imported Aloe vera (L.) Burm.f. (Xanthorrhoeaceae) grown in greenhouses in Korea. This species is native to tropical Africa and probably was introduced into greenhouses via the aloe plant trade. This introduction reinforces the need to focus attention on the eradication from collected localities, and for constant surveillance and detection at ports of entry to prevent introduction and establishment of new pests in the Korean environment. In this paper, additional information for the aloe vera aphid is provided with diagnoses and photographs along with host plant and distribution data for accurate species identification.

• Korean Journal of Plant Resources
• /
• v.22 no.6
• /
• pp.506-512
• /
• 2009

To study the effect of genotype of donor plants on anther culture, anthers of nine hybrid cultivars (Derby, Special, Bossanova, Minipaprika, Fiesta, Boogie, Phenlene, Kufrah, and Clarity) of sweet pepper (Capsicum annuum L.) were cultured in a petridish containing C medium (Sibi, Dumas De Vaulx medium) supplemented with 0.1 mg/L 2,4-D and 0.1 mg/L kinetin, 3% sucrose and 0.32% Phytagel. The cultures were incubated in the dark at $35^{\circ}C$ for seven days, and then cultured at $25^{\circ}C$ with a photoperiod of 16 hr. daylength for 40 days. Frequency of callus formation and plant regeneration was varied among cultivars. Callus formation was ranged from 6% in Phenlene to 69.8% in Kufrah. The highest percentage of regenerated plantlets was obtained in cv. Phenlene (2.67%) followed by Bossanova (2.41%). Result of ploidy analysis; chromosome number observation and flowcytometry analysis, showed that haploid plants could be developed from all of these hybrid cultivars except cv. Fiesta, where highest percentage of haploid plants were obtained in Minipaprika (40%) followed by cv. Bossanova (36.1%). Haploid plants derived from these hybrid cultivars contained single set of chromosome (12 in numbers), higher stomata density (numbers), and smaller sized stomata as compare to diploid plants. The mean length of stomata was 26.9 ${\mu}m$ in haploid plants and 35.7 ${\mu}m$ in diploids.

• Journal of Plant Biotechnology
• /
• v.48 no.3
• /
• pp.156-164
• /
• 2021

Spirodela polyrhiza, from the Lemnaceae family, are small aquatic plants that offer an alternative plant-based system for the expression of recombinant proteins. However, no turion transformation protocol has been established in this species. In this study, we exploited a pB7YWG2 vector harboring the eYFP gene that encodes enhanced yellow fluorescent protein (eYFP), which has been extensively used as a reporter and marker to visualize recombinant protein localization in plants. We adopted Agrobacterium tumefaciens-mediated turion transformation via vacuum infiltration to deliver the eYFP gene to turions, special vegetative forms produced by duckweeds to endure harsh conditions. Transgenic turions regenerated several duckweed fronds that exhibited yellow fluorescent emissions under a fluorescence microscope. Western blotting verified the expression of the eYFP protein. To the best of our knowledge, this is the first report of an efficient protocol for generating transgenic S. polyrhiza expressing eYFP via Agrobacterium tumefaciens-mediated turion transformation. The ability of turions to withstand harsh conditions increases the portability and versatility of transgenic duckweeds, favoring their use in the further development of therapeutic compounds in plants.

• Coupled systems mechanics
• /
• v.11 no.2
• /
• pp.121-132
• /
• 2022

Wastewater treatment plants (WWTPs) are designed and built to remove contaminants from wastewater. WWTPs are composed of various facilities equipped with hydro-mechanical and electrical equipment. This paper presents a comparison of two different approaches for WWTPs modelling. Static modelling is suitable for determining the dimensions of facilities and equipment capacity. The special significance of this approach is for the design of new plants, i.e., when a very small number of input data on the quantities and composition of the influent wastewater is available. Dynamic modelling is expensive, time consuming and requires great expertise in the use of simulators, models and very good understanding of the treatment processes. Also, dynamic modelling is very important to use for optimization, consideration of future scenarios and also possible scenarios on the plant. The comparison of two approaches was made on the input data from the biggest and most important plant in Bosnia and Herzegovina (B&H)-WWTP Butila (Sarajevo). The main idea is to show the differences between two demanding accesses. It is important to know how to apply an adequate approach to research and solve the set task. The II phase of the plant Butila, which includes the removal of nutrients, is planned in several years and therefore the importance of research has increased.

• Journal of Plant Biotechnology
• /
• v.50
• /
• pp.27-33
• /
• 2023

Zizyphus jujube is a plant in the buckthorn family (Rhamnaceae) that has been the subject of research into antibacterial, antifungal and anti-inflammatory properties of its fruit and seed. However, few studies have investigated its leaves. In this study, the anti-inflammatory activity of ZJL (an extract of Z. jujube leaf) was evaluated to verify its potential as an anti-inflammatory agent and SARS-CoV-2 medicine, using nitric oxide (NO) assay, RT-PCR, SDSPAGE, Western blotting, and UHPLC/TOFHRMS analysis. We found that ZJL suppresed pro-inflammatory mediators such as NO, inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and tumor necrosis factor α (TNF-α) in lipopolysaccharide (LPS)-induced RAW264.7 cells. ZJL acted by inhibiting NF-KB and MAPK signaling pathway activity. We also confirmed that ZJL contains a phenol compound and flavonoids with anti-inflammatory activity such as trehalose, maleate, epigallocatechin, hyperoside, catechin, 3-O-coumaroylquinic acid, rhoifolin, gossypin, kaempferol 3-neohesperidoside, rutin, myricitrin, guaiaverin, quercitrin, quercetin, ursolic acid, and pheophorbide a. These findings suggest that ZJL may have great potential for the development of anti-inflammatory drugs and vaccines via inhibition of NF-κB and MAPK signaling in LPS-induced RAW264.7 cells.

• The Plant Pathology Journal
• /
• v.40 no.2
• /
• pp.225-232
• /
• 2024

The microbiomes of two important rice cultivars in Vietnam which differ by their susceptibility to the bacterial leaf blight (BLB) disease were analyzed through 16S rRNA amplicon technology. A higher number of operational taxonomic units and alpha-diversity indices were shown in the BLB-resistant LA cultivar than in the BLB-susceptible TB cultivar. The BLB pathogen Xanthomonas was scantly found (0.003%) in the LA cultivar, whereas was in a significantly higher ratio in the TB cultivar (1.82%), reflecting the susceptibility to BLB of these cultivars. Of special interest was the genus Acholeplasma presented in the BLB-resistant LA cultivar at a high relative abundance (22.32%), however, was minor in the BLB-sensitive TB cultivar (0.09%), raising a question about its roles in controlling the Xanthomonas low in the LA cultivar. It is proposed that Acholeplasma once entered the host plant would hamper other phytopathogens, i.e. Xanthomonas, by yet unknown mechanisms, of which the triggering of the host plants to produce secondary metabolites against pathogens could be a testable hypothesis.