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Discrimination between RNAP IIA and IIO in Preinitiation Complex Assembly and Tyrosine Phosphorylation of the Carboxy Terminal Domain

  • Lee, Sang-Soo
    • BMB Reports
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    • v.30 no.5
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    • pp.362-369
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    • 1997
  • Multiple phosphorylation of the carboxy-terminal domain (CTD) of the largest subunit in RNA polymerase II (RNAP II) is thought to play an important role in the transcription cycle. The preinitiation complex in a partially purified complete transcription system suggested that RNA polymerase IIA containing unphosphorylated CTD is involved in complex assembly, whereas RNA polymerase IIO containing Ser and Thr phosphorylated CTD is not involved in preinitiation complex assembly. Recently a minimal transcription system was developed which requires chemically defined minimal components for its transcription: TBP, TFIIB, TFIIF, RNAP II and a supercoiled adenovirus-2 major late promoter (Ad-2 MLP). It would be using interesting to determine the consequence of CTD phosphorylation on preinitiation complex formation using the minimal transcription system. Contrary to the results from the partially purified complete transcription system, both RNA polymerase IIA and IIO are equally recruited in the preinitiation complex formation. The discrepancy may result from the two different assays used to determine complex formation, the use of chemically undefined complete and defined minimal transcription systems. This implicates that some factors in the complete transcription system are involved in the distinction between RNAP IIA and IIO in complex assembly. In addition multiple tyrosine phosphorylation of the CTD of RNAP II was prepared with c-Abl kinase and its recruiting ability in the preinitiation complex was examined. Compare with Ser and Thr phosphorylated RNAP IIO, Tyr phosphorylated RNAP IlOy forms a stable preinitiation complex in both the minimal and complete transcription systems. Based on these results, it seems that tyrosine phosphorylation of the CTD is important in the transcription cycle on the special subset of class-II promoter or has a different role in the transcription process.

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Development a Two Step Heater Using Induction Heating Based on o High Frequency Resonant Inverter (고주파 공진형 인버터를 이용한 유도가열형 2단 히터)

  • Shin, Dae-Cheul;Kwon, Hyuk-Min
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.19 no.7
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    • pp.81-86
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    • 2005
  • Proposed induction-heated system is innovative system which applied special high-frequency power circuit technique for thermal converse technique and IH(Induction-Heating) magnetic induction heating generated from induction-heated metallic package that is for distillation unit. In this occurs not burning, so that the working environment can be improved. This electromagnetic induction heating technique is used high frequency inverter. By using high frequency inverter high frequency alternative current (HFAC) in the range of [kHz] can be made with conventional alternative current In this contribution IGBT module is used for high frequency inverter. In this paper are discussed action analysis and characteristics analysis of 1.5[kW]-Class half-bridge resonant inverter system and resonant metallic packaged. In addition, by using this system,t how two step heating superheated steam generator is developed and application of system are also discussed.

The Study of Golf Activities of the King Young Chin (영친왕의 골프활동에 관한 연구)

  • Cho, Sang-Woo
    • Journal of the Korean Applied Science and Technology
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    • v.37 no.4
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    • pp.986-995
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    • 2020
  • The purpose of this study is to find out about unknown golf activities through sociological analysis based on the relationship between King Youngchin's life in Japan and the people around him. The following conclusions were drawn. King Youngchin was influenced by the Japanese imperial family to learn golf for the first time in 1924, and participated in golf tournaments in 1925. In 1927, during a one-year European tour, he participated in golf games in Singapore, Paris, Switzerland, England, Belgium, the Netherlands, and Italy, as well as golf course tours, golf lessons, and visiting golf ball manufacturing factories. After returning from Europe, he provided support for the site of Gyeongseong Gunjari Golf Course, construction and operation expenses. However, he did not show any special golf activities during his life as a high class Japanese military officer, and after the Pacific War, he could not find any more golf activities due to social conditions and economic conditions such as wartime restoration. There were no more golf activities due to health problems from 1958.

Marginal Propensity to Consume with Economic Shocks - FIML Markov-Switching Model Analysis (경제충격 시기의 한계소비성향 분석 - FIML 마코프-스위칭 모형 이용)

  • Yoon, Jae-Ho;Lee, Joo-Hyung
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.15 no.11
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    • pp.6565-6575
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    • 2014
  • Hamilton's Markov-switching model [5] was extended to the simultaneous equations model. A framework for an instrumental variable interpretation of full information maximum likelihood (FIML) by Hausman [4] can be used to deal with the problem of simultaneous equations based on the Hamilton filter [5]. A comparison of the proposed FIML Markov-switching model with the LIML Markov-switching models [1,2,3] revealed the LIML Markov-switching models to be a special case of the proposed FIML Markov-switching model, where all but the first equation were just identified. Moreover, the proposed Markov-switching model is a general form in simultaneous equations and covers a broad class of models that could not be handled previously. Excess sensitivity of marginal propensity to consume with big shocks, such as housing bubble bursts in 2008, can be determined by applying the proposed model to Campbell and Mankiw's consumption function [6], and allowing for the possibility of structural breaks in the sensitivity of consumption growth to income growth.

A Study of Korean Skull Base Height - with Special Reference to the MIA Sample during the Korean War and the Late Chosun Sample - (한국인 머리뼈 밑면 높이 연구 - 조선후기인골(17-18세기)과 6.25전사자를 중심으로 -)

  • Park, Sun-Joo
    • The Korean Journal of Quaternary Research
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    • v.21 no.1
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    • pp.37-45
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    • 2007
  • Skull base height increases significantly with better nutrition and health conditions. With coordinate caliper or by subtraction, skull base height is easily measured. To examine nutrition and health condition of MIA(missing in action) during the Korean War and people who lived during the 17-18 centry of the Late Chosun. This study is focused on the change of skull base height through time as seen in comparing 83 MIA cranium and 12 17-18 centry cranium of Korea with 219 modern American middle class adult cranium(Terry Collection and forensic skeleton). To sum up, nutritional condition of MIA is not significantly changed, as seen in comparing with nutritional condition of 17-18 century people.

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Development of supplemental nutrition care program for women, infants and children in Korea: $NutriPlus^+$

  • Kim, Cho-Il;Lee, Yoon-Na;Kim, Bok-Hee;Lee, Haeng-Shin;Jang, Young-Ai
    • Nutrition Research and Practice
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    • v.3 no.3
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    • pp.171-179
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    • 2009
  • Onto the world-fastest ageing of society, the world-lowest fertility rate prompted a development of various policies and programs for a betterment of the population in Korea. Since the vulnerability of young children of low socio-economic class to malnutrition was clearly shown at the in-depth analysis of the 2001 Korea National Health and Nutrition Examination Survey data, an effort to devise supplemental nutrition care program for pregnant/breastfeeding women, infants and preschool children was initiated. The program was designed to offer nutrition education tailored to fit the needs of the participants and special supplementary foods, using USDA WIC program as a benchmark. Based on the dietary intake of those age groups, target nutrients were selected and their major food sources were searched through nutrient content of foods and dietary pattern analysis. As a result, we developed 6 kinds of food packages using combinations of 11 different food items. The amount of each item in a food package was determined to supplement the intake deficit in target nutrients. Nutrition education in $NutriPlus^+$ aims to improve the nutrition knowledge, attitude, and dietary behaviors of the participants, and is provided through group lessons, individual counseling sessions and home visits. Breastfeeding is promoted with top priority in education for the health of both mother and baby. The eligibility guidelines were set for residency, household income, age, pregnancy/breastfeeding and nutritional risk such as anemia, stunting, underweight, and/or inadequate nutrient intake. Income eligibility was defined as household income less than 200 percent of the Korean poverty guidelines. A pilot study to examine the feasibility of program implementation was run in 3 public health centers in 2005 and expanded to 15 and 20 in the following 2 years. The result of 3-year pilot study will be reported separately along with the ultimate nationwide implementation of the $NutriPlus^+$ in 2008.

A Study on the Optimum Structural Design of Naval Vessels (함정의 최적 구조 설계에 관한 연구)

  • Seung-Il Seo;Keon-Ho Son;Myung-Kyu Park
    • Journal of the Society of Naval Architects of Korea
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    • v.39 no.1
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    • pp.100-112
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    • 2002
  • Naval vessels are not regulated by the class rules, but by the special regulations. This study introduces the concept and characteristics of the regulations of U.S. Navy which has been the most reliable standards in design of naval vessels in Korea, and intends to help designers to comprehend the effect of each regulation on design results. Also, an optimum structural design method combined with the structural analysis theory is proposed for naval vessels following the regulations of U.S. Navy and is applied to the design of a naval vessel. After application of the optimum design method, its validity is shown and an optimum design of midship section is obtained. In addition, the optimum spaces or longitudinals and transverse web frames are found and the effect of main design variables can be investigated.

A Study on the Effects of Korean Restaurant′s Menu Price Policy upon the Sale(The Case of ‘B’ Korean Restaurant of ‘P’ Hotel at Seoul) (한식당 가격정책이 매출에 미치는 영향에 관한 연구)

  • 김희기
    • Culinary science and hospitality research
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    • v.9 no.2
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    • pp.148-175
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    • 2003
  • As mentioned above, Korean restaurant's menu price is to be decided by combining various factors. Korean restaurant is demanded to decide menu price more carefully than other restaurants do, because the number of Korean restaurant is the largest in Korea and Korean food is the most popular among the Korean people. Korean restaurant cannot neglect price competitiveness and is often given complaints from its customers. Because of such management difficulties, the number of Korean restaurant has recently decreased at special class hotels as well as common hotels. Korean restaurant managers are demanded to make efforts to inherit Korean tradition and culture by keeping pride and responsibility. Until now, Korean restaurants are thought to decide the menu prices in short-sighted, non-scientific and haphazard way of thinking. Such price decision factors have established traditions and have been generally accepted to let Korean restaurant managers lose carefulness at the menu price decision. In advanced countries, however, they recognized the importance of the price decision since the 1960's or earlier to research the menu price systematically and scientifically. Before deciding the menu prices, Korean restaurant managers are demanded to investigate various kinds of factors carefully and spend a lot of time to calculate direct costs, that is, one of the most important factors of cost calculations. The managers are demanded to decide the menu prices in reasonable and future-oriented way by keeping not private thinking but correct information and judgment. The sale of each menu has difference, while the menu price increase has been evaluated to be successful from overall point of view. Despite of increased total sale, there was not much difference of net profit because of increased material costs. However, higher level of the customers produced comfortable and cozy atmosphere of the restaurant enough to satisfy customers, and improved service quality much more. Not only customer satisfaction but also improved service quality is thought to play an important role in invitation of future customers.

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A Study on the Use of Health Family Support Centers and Its Activation Plan - Focused to Gyeonggi-do - (건강가정지원센터 이용실태 및 활성화 방안 연구 - 경기도를 중심으로 -)

  • Yang, Jung-Sun;Kim, Sung-Hee
    • Journal of Family Resource Management and Policy Review
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    • v.14 no.4
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    • pp.213-237
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    • 2010
  • This study aimed at figuring out the current state of 26 family-health support centers in Gyeonggi-do, in order to prepare an activation plan for the centers. These centers are the main mediators of the family health operation. This study also aimed to understand the center users' satisfaction and demands. The results were as follows. First, a total of 65,794 participants from 9,960 businesses were processed. The majority had attended various integrated family services, followed by family counseling and/or family-friendly culture-establishing businesses. Second, the main users' profiles were as follows: average age, 41; average number of persons in the family, 3.78; education above foundation-level degree; diverse jobs that included special occupations; average monthly incomes of 3,390,000 won; and a middle-class living standard (as self-described by participants). Third, the users' main concern for their families was "financial difficulties," and they had an interest in education and counseling. Although they thought education and counseling were suitable solutions, they hesitated because they did not think their problems were serious enough to merit them. Fourth, the satisfaction level of their participation in their center's program was generally high, at 3.9-4.2 points. The majority of them said they would attend again in the future.

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The Effect of Head Posture Change on Initial Occlusal Contacts (두부의 자세 변화가 초기 교합접촉에 미치는 영향)

  • Woo-Cheon Kee
    • Journal of Oral Medicine and Pain
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    • v.20 no.1
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    • pp.195-204
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    • 1995
  • The purpose of this study was to evaluate effect of head posture change on initial occlusal contacts through measuring the distances between initial occlusal contacts and maximum intercuspal position at different head posture. Two special devices were designed and constructed. Mandibular movement replicator was used to assess reliability of the K6 diagnostic system(MKG; Myo-tronic Inc, Seatle, USA) and head posture calibrator was used to maintain the constant head posture during experiment. We measured difference of distance between initial occlusal contact and maximum intercuspal position with MKG in upright, supine, 45 degrees extension, 30 degrees flexion, 30 degrees right and left bending postion of the head. The Frankfurt horizontal plane was used as a reference plane. 21 adults aged from 23 to 25 were selected, who have normal or class I molar relationship, and have no symptoms on TMJ and masticatory muscles, and have restorations less than 3 surfaces on each tooth, and have no other prosthetic restoration. The obtained results were as follows : The mean absolute distances between initial occlusal contact and maximum intercuspal postion were 0.39(0.18mm in the upright position, 0.65(0.37mm in the supine position, 0.59(0.33mm in the 45 degree extension, 0.70(0.53mm in the 30 degrees flexion, 1.12(1.10mm in the 30 degrees right bending and 1.94(0.67mm in the 30 degrees left bending of the head. The positions of the initial occlusal contacts have a tendency to locate anterior, left and inferior to maximal intercuspal position in upright position, posterior and inferior in supine position and 45 degrees extension, anterior and inferior in 30 degrees flexion, right and inferior in 30 degrees right bending, and left and inferior in 30 degrees left bending of the head. There were significant differences among the initial occlusal contacts in each head postures(P<0.0001). Therefore, we need to check initial occlusal contacts in the altered head posture during occlusal analysis and adjustment of occlusal appliance and dental occlusion for diagnosis and treatment of temporomandibular disorder.

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