• Journal of Life Science
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• v.11 no.3
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• pp.235-241
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• 2001

To select the sntagonistic bacteria against B. cinerea, isolates were screened from the eggplant leaves and rhizosphere soils in the eggplnat fields in the greenhouse. W1 and P99 isolates were selected by the inhibition of mycelial growth of B. cinerea E12 in vitro test. These isolates, W1 and P99, were identified as Bacillus subtilis and Pseudomonas putida, respectively, by the Bergeys manual and API systems, For the formulation of the antagonistic bacteria, the media for the mass production were prepared with biji(soybean curd residues) or soybean flour. B. subtilis W1 or P. putida P99 was mass cultured in biji broth or soybean flour extrect broth and then soybean flour, corn starch flour, rice glutinous flour and biji flour as high molecular substrates were added. These mixtures were dried, grinded and formulated as brofungicides of wettable powder type. The assess the control effect of biofungicides against the infection of B. cinerea, six types of formulations were assayed at the pot culturing with eggplant in the greenhouse. According to the results, there were no significant differences among the formulation methods. However, P99S or PppB formulated with P. putida P99 showed the highest control values as 90.4% and 96.1%, respectively. Then. BSB or BSD formulated whit B. subtilis W1 were 80.8% and 83.0%, respectively. There afforementioned values were more effective than that of chemical fungicide. Ipro W.P which showed as 72.6%.

• Molecules and Cells
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• v.23 no.3
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• pp.280-286
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• 2007

L-asparaginase (EC 3.5.1.1) catalyzes the hydrolysis of the amide group of L-asparagine, releasing aspartate and $NH_4{^+}$. We isolated a low temperature-inducible cDNA sequence encoding L-asparaginase from soybean leaves. The full-length L-asparaginase cDNA, designated GmASP1, contains an open reading frame of 1,258 bp coding for a protein of 326 amino acids. Genomic DNA blotting and fluorescence in situ hybridization showed that the soybean genome has two copies of GmASP1. GmASP1 mRNA was induced by low temperature, ABA and NaCl, but not by heat shock or drought stress. E. coli cells expressing recombinant GmASP1 had 3-fold increased L-asparaginase activity. A possible function of L-asparaginase in the early response to low temperature stress is discussed.

• Korean Journal of Environmental Agriculture
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• v.15 no.1
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• pp.19-24
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• 1996

To investigate the alterations of ultrastructure of leaves by acid rain, 10mm of SAR(Simulated Acid Rain, pH 2.0, 2.5, 2.7, 3.0, 6.0) were applied to 4 crops(rice, soybean, barley, and radish) at the two day interval. The symptoms of leaf damage by SAR were observed by naked eyes and SEM(Scanning Electron Microscope). The results are summarized as follow: Visible leaf injury were more serious in dicots than monocots such as rice and barley with the order of bean, radish, rice, barley. With the SAR treatment of pH 2.0, histological perturbation of trichome were developed in bean and radish. And with the SAR treatment of pH 2.5, stomata of all tested crops except rice were deshaped.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 1993.10a
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• pp.637-647
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• 1993

Over 40 per cent Indian population needs to be attended for nutritional improvement. Traditional nutritious food resources though abundantly available but presently less used, if incorporated in the diets, can yield low-cost nutritionally balanced diets. The linear programming (LP)model was used for optimization of food resources to satisfy protein -energy requirements of hard working rural poor. Soybean-a highly nutritious food legume-available at reasonably low price was included along with chick -pea and pigeon -pea for comparison .The three cereals predominantly used in Indian diets -rice, wheat and sorghum -and three typical leafy vegetables namely, spinach , drumstick leaves and rajagira (Amaranths paniculantus) leaves were used for optimization . The contribution of food legume was restricted to supply only 50 per cent of daily protein requirement for maximum protein value. The quantities of other food stuffs namely , milk , sugar , tubers etc. were restricted to nati nal per capita availability. the nutrition satisfaction levels in per cent RDA by the model are 149-250 for protein , 51-106 for fat, 134-362 for iron an d143-1158 for Vitamin-A. Quantities of food legumes, cereals and leafy vegetables ranged from 289-601, 3000-3700 and 360-1200 g/day respectively for a reference family of five hard working rural poor. Daily food resources cost ranged from US$ 0.6 to 1.0 for the family. Amongst food legumes, soybean based diet combinations provide maximum nutritional benefits at minimum cost indicating its suitability for wider adoption by hard working poor.

• The Plant Pathology Journal
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• v.20 no.1
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• pp.46-51
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• 2004

Host resistance is usually parasite-specific and is restricted to a particular pathogen races, and commonly is expressed against specific pathogen genotypes. In contrast, resistance shown by an entire plant species to a species of pathogen is known as non-host resistance. Therefore, non-host resistance is the more common and broad form of disease resistance exhibited by plants. As a first step to understand the mechanism of non-host plant defense, expressed sequence tags (EST) were generated from a hot pepper leaf cDNA library constructed from combined leaves collected at different time points after inoculation with non-host soybean pustule pathogen (Xanthomonas axonopodis pv. Glycines; Xag). To increase gene diversity, ESTs were also generated from cDNA libraries constructed from anthers and flower buds. Among a total of 10,061 ESTs, 8,525 were of sufficient quality to analyze further. Clustering analysis revealed that 55 ％ of all ESTs (4685) occurred only once. BLASTX analysis revealed that 74％ of the ESTs had significant sequence similarity to known proteins present in the NCBI nr database. In addition, 1,265 ESTs were tentatively identified as being full-length cDNAs. Functional classification of the ESTs derived from pathogen-infected pepper leaves revealed that about 25％ were disease- or defense-related genes. Furthermore, 323 (7％) ESTs were tentatively identified as being unique to hot pepper. This study represents the first analysis of sequence data from the hot pepper plant species. Although we focused on genes related to the plant defense response, our data will be useful for future comparative studies.

• Korean journal of applied entomology
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• v.36 no.1
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• pp.48-54
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• 1997

This study was conducted to determine the effect of temperatures and food sources on the egg and larval developmentof the tobacco cutworm, Spodoptera litura Fabricius. The hatchability of egg masses of S. liturawas 100% on the leaf of soybean, perilla and sweet potato in any given temperature regimes, while the hatchabilitywas only 65-8796 when reared on the pulp paper and decreased as temperature increased. Egg durationwas not significantly different among different food sources within each temperature. However, egg duration at32$^{\circ}$C was shorter than that at 24$^{\circ}$C and 28$^{\circ}$C. During the early larval development, at 28$^{\circ}$C and 32$^{\circ}$C the larvafed on sweet potato leaf was heavier than those fed on soybean and perilla leaves and the opposite case wastrue during mid-larval development stage. However, larval weight at 24$^{\circ}$C was heavier on sweet potato leafthan that on soybean and perilla leaves until 12 days after hatching. This result was probably due to relativelyslower developmental rate at 24$^{\circ}$C compared to 28$^{\circ}$C and 32$^{\circ}$C. The mean larval mortality was 68.896, 44.5%and 33.8% at 24$^{\circ}$C. 28$^{\circ}$C and 32"C, respectively. The lowest mortality was observed on soybena leaf and followedby perilla and sweet potato leaves, and artificial diet regardless of temperature conditions. The durationwas the shortest when they fed on soybean leaf, and followed by perilla and sweet potato leaves and artificialdiet. Larval durations were 23.6-30.4 days at 24$^{\circ}$C. 18.6-22.3 days at 28$^{\circ}$C and 14.5-18.0 days at 32$^{\circ}$C. Thethreshold temperatures of egg and larva of S. litura were about 6.l"C and 10.9"C, respectively.t 6.l"C and 10.9"C, respectively.pectively.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.2
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• pp.96-102
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• 2003

This study was conducted to determine the effects of two plant populations (28 and 14 plants per $m^2$) and two toppings in conventional plant population (28 plants per $m^2$) on soybean (Glycine max L. cv. Pungsannamulkong) cultivated in the paddy field. The two topping time were taken at 6$^{th}$ to 7$^{th}$ and 8$^{th}$ to 9$^{th}$ trifoliolate leaf stages in the conventional plant population. Experimental design for growth data was a randomized complete block with three replications, and samples were taken at R1 (July 31), R3 (August 19), R5 (September 2) and R7 (September 23) growth stages. The branch number of soybean was relatively higher in the low plant population (14 plants per $m^2$) and with the topping at the 6$^{th}$ to 7$^{th}$ leaf stage, in the conventional plant population (28 plants per $m^2$), and with topping at the 8$^{th}$ to 9$^{th}$ trifoliolate leaf stage in descending order. The highest average branch length of soybean was observed in the low population and the longest branch length was observed from the soybean with topping at the 6$^{th}$ to 7$^{th}$ leaf stage. The leaf number per plant was decreased in order of in the low population, with the topping at 6$^{th}$ to 7$^{th}$ trifoliolate leaf stage, with the topping at 8$^{th}$ to 9$^{th}$ trifoliolate leaf stage, and in the conventional population. The leaf area was high in the low population and with topping at 6$^{th}$ to 7$^{th}$ trifoliolate leaf stage and was relatively low in the conventional population and with the topping at 8$^{th}$ to 9$^{th}$ trifoliolate leaf stage in soybean. The dry weight of leaves and branches was high in the low population and with the topping at 6$^{th}$ to 7$^{th}$ trifoliolate leaf stage and was relatively low in the conventional population and with topping at 8$^{th}$ to 9$^{th}$ trifoliolate leaf stage. The leaf number per plant was high in the low population and with topping at 6$^{th}$ to 7$^{th}$ trifoliolate leaf stage and was relatively low in the conventional population and with topping at 8$^{th}$ to 9$^{th}$ trifoliolate leaf stage. The grain yield per 10a was high with the topping at 6$^{th}$ to 7$^{th}$ trifoliolate leaf stage.

• Journal of the Korea Organic Resources Recycling Association
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• v.14 no.4
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• pp.132-140
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• 2006

This investigation into unfermented soybeans, which were left from the soybeans used to make cheongguk-jang, was conducted to find out its availability of the application to the environmental-friendly fertilizers. The test of cultivating lettuce, using 1/5000a Wagner pot, was carried out inside the affiliated farm glasshouse, belonged to University of Chung-Nam National. The results showed that in the category of leaf length, the control and cheongguk-jang soybeans treatment plot had similar outcomes and in the category of the extract of the cheongguk-jang soybeans, it increased by about 26~33%. And in the categories of width and number of leaves it showed that the extract treatment increased by about 9~20%, in compared to the control. Finally, in the category of fresh weight, the results showed that cheongguk-jang soybean and the extract of the cheongguk-jang soybean effectively increased by about 9~27%, and that pig manure compost treatment plot with the extract of the cheongguk-jang soybean also significantly increased by about 26%, compared to the control. Accordingly, the unfermented soybean left in the production of cheongguk-jang and the extract of the cheongguk-jang soybean are considerably appeared to be valuable environmental-friendly fertilizers.

• Korean Journal of Environmental Agriculture
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• v.2 no.1
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• pp.30-34
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• 1983

The present study was carried out to determine the effect of a radionuclide, cesium-137, in soybean, which is an element released usually from nuclear facilities. Soybean plants were grown on the pots treated with cesium-137 $0.5{\sim}60{\mu}Ci/1kg$ soil and the uptake, translocation and accumulation of the radiocesium in the plant parts were measured at different growth stage. The results are summarized as follows: 1) Visual toxic symptoms on the plants due to treatment of radioactive cesium were not observed up to $60{\mu}Ci/10Kg$ soil in a pot. 2) The uptake of cesium-137 in soybean plant was increased with increment of concentration applied, while the uptake of potassium was proportionally decreased, indicating to have an ion antagonistic relationship between them. 3) The absolute amounts of cesium-137 in the plants were gradually increased by the pod setting stage, but rather reduced at harvesting stage. The accumulation occurred more in the leaves and stems than the soybean seeds. 4) The rate of uptake was ranged from 0.069 to 0.005 with proportional decrease by increasing concentration applied and the rate of Cs-137 translocation from plants to seeds was averaged 38.6% in soybean plant. The concentration coefficient was 0.04 in the soybean seeds from the pots treated with $20{\mu}Ci$ of cesium-137 and decreased with increment of cesium-137 applied.

• Journal of the Korean Society of Tobacco Science
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• v.1 no.1
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• pp.33-38
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• 1979

A simple test using latex-agglutination was developed to detect serologically tobacco mosaic virus (TMV), soybean mosaic virus (SoyMV), and barley stripe mosaic virus (BSMV) from infected Plants. Latex spheres ( 0.81 $\mu$, Difco) were adsorbed with immuno globulin purified by electrophoresis from crude antiserum against viruses. The antibody- sensitized latex suspension was mixed with sap from virus -infected leaves in a glass capillary tube (inner diam. 1mm $\times$ 100 mm length) The mixture, after agitation, was observed under a stereo microscope at low magnification (X20 - X4O), to examine the reaction between antigen (Virus) and its antibody. Flocculation occurred when the reaction was positive.