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Biocontrol of Southern Blight Caused by Sclerotium rolfsii in Pepper Plants Using Bacillus subtilis GJ6-14

  • Hae Jung Moon;Mee Kyung Sang
    • Research in Plant Disease
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    • v.30 no.2
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    • pp.181-188
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    • 2024
  • Southern blight, caused by the soil-borne fungus Sclerotium rolfsii, is a serious disease that affects many economically important crops. In this study, we selected Bacillus subtilis GJ6-14, from a total of 260 strains, to control Southern blight in pepper plants. In both seedling and plant tests, GJ6-14 significantly suppressed disease incidence and severity compared to control, furthermore, GJ6-14 demonstrated efficient colonization in the rhizosphere by maintaining the population from log 5.41 to log 3.92 in the pathogen-inoculated plants, indicating its potential as a biocontrol agent. Molecular analysis revealed up-regulation of defense-related genes, such as a 7.6-fold increase in LOX1 and 15.5-fold increase in PR1, at 72 hr after inoculation of S. rolfsii in GJ6-14-treated plants, suggesting activation of plant defense mechanisms. Overall, our findings highlight the promising role of B. subtilis GJ6-14 as a potential biocontrol agent in sustainable management of Southern blight in pepper plants.

The Duration and H/V ratio of the Ground Motion in Southern Korea (남한 지진의 지속시간과 H/V 비율)

  • 최호선;박창업;조남대
    • Proceedings of the Earthquake Engineering Society of Korea Conference
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    • 2002.03a
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    • pp.42-50
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    • 2002
  • The duration and H/V ratio(the amplitude ratio of the horizontal to vertical components) of ground motions caused by earthquakes in southern Korea are analyzed. Total 329 seismograms of horizontal component recorded at hypocentral distances of 10 to 350 km from 27 earthquakes with local magnitude 2 to 4 are used for the analysis. Simplified relation between the duration of ground motion( $T_{d}$) and the ratio($\chi$) of Arias intensity( $I_{A}$) and squared maximum acceleration($\alpha$$_{max}$$^{2}$) is determined to be $T_{d}$ = 3.423$\chi$$^2$+ 8.200$\chi$ + 0.029, which is useful for the estimation of the duration in southern Korea. There are three distinct distance ranges with different linear variations of the duration in hypocentral distance. They are distance intervals of 10~80km, 80~140km, and the distance greater than 140km. The duration in southern Korea shows clear proportionality to the local magnitude at magnitudes greater than 3.1. The value 1.37 of the H/V ratio obtained in southern Korea is similar to the value 1.4 of ENA(Eastern North America). The H/V ratio in southern Korea increases in the frequency range from 0.3 to 10Hz. The duration and H/V ratio of ground motions derived in this study could be used in the stochastic simulation of strong ground motion.ion.n.n.

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Determination of the PDE-5 Inhibitors and Their Analogues by GC-MS and TMS Derivatization

  • Pyo, Jae-Sung;Lee, Hee-Sang;Park, Yu-Jin;Jo, Ji-Yeong;Park, Yong-Hoon;Choe, Sang-Gil;Lee, Mi-Young;Lee, Jae-Sin
    • Mass Spectrometry Letters
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    • v.3 no.1
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    • pp.15-17
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    • 2012
  • Eighteen of the PDE-5 inhibitors and their analogues were analyzed using GC-EI-MS. Fourteen of them could be identified by simple GC-MS method without derivatization, but hydroxyhongdenafil, hydroxyvardenafil, xanthoanthrafil and mirodenafil could not be identified without derivatization for the high polarity due to the presence of hydroxyl groups. N,O-bis(trimethylsilyl) trifluoroacetamide (BSTFA) and N-methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide (MTBSTFA), widely used trimethylsilyl (TMS) derivatizing reagents, were used to improve the sensitivity of the hydroxylated analogues. And the analytes could be identified by GC-MS after the derivatization.

Water Balance-based Farmland Suitability for Southern-type Garlic Cultivation (난지형 마늘의 농업수리학적 재배적지 분류)

  • Kim, Yong Wan;Hong, Suk Young;Kim, Yi Hyun;Jang, Min Won
    • Journal of The Korean Society of Agricultural Engineers
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    • v.54 no.6
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    • pp.19-28
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    • 2012
  • This study carried out farmland suitability analysis for southern-type garlic cultivation considering soil and temperature as well as water deficit conditions. The spatial extent was limited within the area derived by Kim et al. (2012) using just soil and temperature constraints. Daily soil moisture was simulated using a one-layer soil water balance model at a $100{\times}100m$ grid unit, and then annual water deficit was calculated from 2000 to 2010. The farmland suitability was classified as four steps: best suitable, suitable, possible, and low productive. As a result, total area of best suitable or suitable farmland was about 375,900 ha, and Gimje-si and Haenam-gun were appeared as the largest favorable area for southern-type garlic cultivation. The best suitable or suitable area at Haenam-gun, Goheung-gun, Shinan-gun, Namhae-gun, and Muan-gun, major production regions of southern-type garlic, were extracted as 20,187 ha, 13,018 ha, 4,715 ha, 1,319 ha, and 349 ha, respectively. On the other hand, the result showed that the adoption of sprinkler irrigation systems might be critical in cultivating the southern-type garlic at some regions having poor water balance.

Assessment on Design Applicability of Analysis of the Undrained Shear Strength in Korea Coastal Marine Clay (국내 해성점토의 비배수 전단강도 분석을 통한 설계 적용성 평가)

  • Kim, Myeong Hwan;Song, Chang Seob
    • Journal of The Korean Society of Agricultural Engineers
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    • v.58 no.1
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    • pp.61-71
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    • 2016
  • This study performed the physical and mechanical experiment on the samples of costal marine clays individually collected in western and southern regions to identify the characteristics of western and southern costal marine clay. Based on the experiment result, the characteristics of costal marine clay is identified undrained shear strength. Based on the experiment result on the physical and mechanical characteristics of costal marine clays, the regression is presented that can analyze the mechanical characteristics of undrained shear strength in costal marine clay of Korea, region of Korea and western-southern region. The correlation of uniaxial compressive strength and undrained shear strength was suitable for use of western-southern region correlation equation. The test result of Jeonnam Yeosu area compares with prediction results of previous researchers formula and western-southern region formula. Prediction results appear highest reliability on the 0.827 of coefficient of determination in the prediction results of the western-southern region formula.

Total Phenolic Contents and Biological Activities of Korean Seaweed Extracts

  • Kim, So-Jung;Woo, Seon-Ock;Yun, Hee-Young;Yum, Seung-Shic;Choi, Eun-Seok;Do, Jeong-Ryong;Jo, Jin-Ho;Kim, Dong-Giun;Lee, Suk-Chan;Lee, Taek-Kyun
    • Food Science and Biotechnology
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    • v.14 no.6
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    • pp.798-802
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    • 2005
  • Crude extracts of thirty seaweeds collected in Korea were obtained using 50% ethanol, and total phenolic contents and antioxidant activities were compared. Two brown algae, Ecklonia cava (E. cava) and Sargassum siliquastrum (S. siliquastrum), showing high antioxidant activity based on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and superoxide anion radical scavenging activity were further investigated for their inhibitory effects on tyrosinase activity. The E. cava extract had the highest total phenolic content among the seaweeds extracts. Total phenolic contents were strongly correlated with antioxidant activity in the thirty seaweed extracts ($R^2\;=\;0.9169$). The E. cava and S. siliquastrum extracts exhibited higher inhibition to tyrosinase activity than butylated hydroxytoluene (BHT) and epigallocatechin gallate (EGCG).

Hybrid genetic-paired-permutation algorithm for improved VLSI placement

  • Ignatyev, Vladimir V.;Kovalev, Andrey V.;Spiridonov, Oleg B.;Kureychik, Viktor M.;Ignatyeva, Alexandra S.;Safronenkova, Irina B.
    • ETRI Journal
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    • v.43 no.2
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    • pp.260-271
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    • 2021
  • This paper addresses Very large-scale integration (VLSI) placement optimization, which is important because of the rapid development of VLSI design technologies. The goal of this study is to develop a hybrid algorithm for VLSI placement. The proposed algorithm includes a sequential combination of a genetic algorithm and an evolutionary algorithm. It is commonly known that local search algorithms, such as random forest, hill climbing, and variable neighborhoods, can be effectively applied to NP-hard problem-solving. They provide improved solutions, which are obtained after a global search. The scientific novelty of this research is based on the development of systems, principles, and methods for creating a hybrid (combined) placement algorithm. The principal difference in the proposed algorithm is that it obtains a set of alternative solutions in parallel and then selects the best one. Nonstandard genetic operators, based on problem knowledge, are used in the proposed algorithm. An investigational study shows an objective-function improvement of 13%. The time complexity of the hybrid placement algorithm is O(N2).