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http://dx.doi.org/10.5478/MSL.2012.3.1.015

Determination of the PDE-5 Inhibitors and Their Analogues by GC-MS and TMS Derivatization  

Pyo, Jae-Sung (Southern District Office of National Forensic Service)
Lee, Hee-Sang (Southern District Office of National Forensic Service)
Park, Yu-Jin (Southern District Office of National Forensic Service)
Jo, Ji-Yeong (Southern District Office of National Forensic Service)
Park, Yong-Hoon (Southern District Office of National Forensic Service)
Choe, Sang-Gil (Southern District Office of National Forensic Service)
Lee, Mi-Young (Industrial Safety and Health Research Institute)
Lee, Jae-Sin (Narcotics Analysis Division of National Forensic Service)
Publication Information
Mass Spectrometry Letters / v.3, no.1, 2012 , pp. 15-17 More about this Journal
Abstract
Eighteen of the PDE-5 inhibitors and their analogues were analyzed using GC-EI-MS. Fourteen of them could be identified by simple GC-MS method without derivatization, but hydroxyhongdenafil, hydroxyvardenafil, xanthoanthrafil and mirodenafil could not be identified without derivatization for the high polarity due to the presence of hydroxyl groups. N,O-bis(trimethylsilyl) trifluoroacetamide (BSTFA) and N-methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide (MTBSTFA), widely used trimethylsilyl (TMS) derivatizing reagents, were used to improve the sensitivity of the hydroxylated analogues. And the analytes could be identified by GC-MS after the derivatization.
Keywords
PDE-5 inhibitor; GC-MS; BSTFA; MTBSTFA; TMS derivatization;
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