Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Biocontrol of Southern Blight Caused by Sclerotium rolfsii in Pepper Plants Using Bacillus subtilis GJ6-14

  • Hae Jung Moon (Division of Agricultural Microbiology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Mee Kyung Sang (Division of Agricultural Microbiology, National Institute of Agricultural Sciences, Rural Development Administration)
  • Received : 2024.04.23
  • Accepted : 2024.05.20
  • Published : 2024.06.30
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Abstract

Southern blight, caused by the soil-borne fungus Sclerotium rolfsii, is a serious disease that affects many economically important crops. In this study, we selected Bacillus subtilis GJ6-14, from a total of 260 strains, to control Southern blight in pepper plants. In both seedling and plant tests, GJ6-14 significantly suppressed disease incidence and severity compared to control, furthermore, GJ6-14 demonstrated efficient colonization in the rhizosphere by maintaining the population from log 5.41 to log 3.92 in the pathogen-inoculated plants, indicating its potential as a biocontrol agent. Molecular analysis revealed up-regulation of defense-related genes, such as a 7.6-fold increase in LOX1 and 15.5-fold increase in PR1, at 72 hr after inoculation of S. rolfsii in GJ6-14-treated plants, suggesting activation of plant defense mechanisms. Overall, our findings highlight the promising role of B. subtilis GJ6-14 as a potential biocontrol agent in sustainable management of Southern blight in pepper plants.

Keywords

Acknowledgement

This work was supported by the National Institute of Agricultural Sciences (Project No. PJ01505102), Rural Development Administration, Republic of Korea.

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