• Korean Journal of Soil Science and Fertilizer
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• v.41 no.5
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• pp.310-317
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• 2008

This study was carried out to investigate the factors of desalinization of the rainfall interception culture soils of Rubus sp. in Gochang-gun, Jeollabuk-do. Soil samples were collected from 85 different sites of the rainfall interception culture soils of Rubus sp. in Gochang-gun, Jeollabuk-do. The electrical conductivity in paste saturation of rainfall interception culture soils ranged from $1.0\sim28.4dS\;m^{-1}$ (average: $4.8dS\;m^{-1}$) and salt affected soil which EC was higher than $4dS\;m^{-1}$, covered nearly 55% of all field surveyed. Salts in rainfall interception culture soils were accumulated by increasing the cultivation period. Electrical conductivity in rainfall interception culture soils was positively correlated with water soluble anions such as chloride ion ($r=0.85^{**}$), nitrate ion ($r=0.94^{**}$), phosphate ion ($r=0.88^{**}$), and sulfate ion ($r=0.84^{**}$), respectively. As a result of desalinization experiments carried out by water management practices, the rinsing method was more effective than leaching method.

• Korean Journal of Poultry Science
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• v.24 no.1
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• pp.17-28
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• 1997

This experiment was carried out to investigate the effects of different chilling and packing methods on physico-chernical properties of cold-stored chicken breast and thigh meats. Dehoned chicken breast and thigh meats were chilled either air spray or ice-water immersion method. The chilled meats were either vacuum packed or atmosphere packed, and stored at -2˚C for 1, 3, 7, 11, 15, and 20 days. The pH of both immersion chilled meats and vacuum packed meats were higher than those of their counterparts(P<0.05). The pH of atmosphere packed meats increased as the storage period extended. The moisture contents of vacuum packed meats were remarkably higher than those of atmosphere packed meats. The pH of all treatments decreased as the storage period extended. The shear values of air spray chilled and vacuum packed breast meats were significantly higher than immersion chilled and vacuum packed ones. However, immersion chilled and atmosphere packed breast meats were significantly higher than those of air spray chilled and atmosphere packed breast meats. The shear values of immersion chilled and vacuum packed thigh meats were significantly higher than those of immersion chilled and vacuum packed thigh meats. In atmosphere packed thigh meats, air spray chilling method showed higher shear values than those of immersion chilled thigh meats. In thigh muscle, tenderness values tended to decrease as the storage period extended(P<0.05). Contents of water soluble proteins of vacuum packed and air spray chilled breast and thigh meats were higher than those of their counterparts as the storage period extended(P<0.05). The contents of water soluble proteins significantly decreased as the storage period extended. Salt soluble proteins of atmosphere packed breast and thigh meats were remarkably higher than those of vacuum packed ones(P<0.05). Total lipid contents of atmosphere packed and air spray chilled breast and thigh meats were higher than those of atmosphere packed and immersion chilled meats as the storage period extended. The vacuum packed meats were significantly higher in total lipid contents than those of atmosphere packed meats. The storage period decreased the total lipid contents of cold chicken, Major fatty acids in cold-stored chicken were oleic, palmitic, linoleic and stearic acids, regardless of chilling method. Unsaturated fatty acids of all treatments decreased, but saturated fatty acids increased as the storage period extended.

• The Korean Journal of Mycology
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• v.32 no.1
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• pp.23-30
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• 2004

Neutral salt-soluble, hot water-soluble and methanol-soluble materials (hereinafter referred to Fr. NaCl, Fr. HW and Fr. MeOH, respectively) were extracted from sclerotium of Grifola umbellata. The Fr. NaCl and Fr. HW did not show any direct cytotoxicity against NIH3T3, Sarcoma 180 and MCF-7, but Fr. MeOH showed cytotoxicity against these cell lines at the concentration of $1,000\;{\mu}g/ml$. Intraperitoneal injection with Fr. NaCl showed antitumor effect with life prolongation of 66.7% and decrease the number of Sarcoma 180 cells of 54.2% in mice inoculated with Sarcoma 180. Fr. NaCl improved the immunopotentiation activity through alternative complement pathway and the alkaline phosphatase activity by $85.05{\sim}88.73%$ and 6 folds, respectively. The number of peritoneal exudate cells and the circulating leucocytes were increased by 1.7 and 3.6 folds in the Fr. NaCl treating group compared with the control group, respectively. The weight of immunoorgans such as liver, spleen and thymus were also gradually increased. The hematological analysis of the Fr. NaCl group was similar with that of the control group. The total polysaccharide and protein contents of Fr. NaCl were 98.25% and 1.44%, respectively. These results indicate that the antitumor activity of Fr. NaCl was exerted through immunopotentiation, but not through cytotoxicity against the tumor cells.

• Journal of Life Science
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• v.18 no.9
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• pp.1290-1298
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• 2008

Tricholoma matsutake, one of edible and medicinal mushroom belonging to Tricholomaceae of Agaricales, has been known to contain some curing effect on gastric cancer and ulcer, and inhibitory effect on sarcoma 180 and Ehrlich sarcoma. Neutral salt soluble (0.9% NaCl), hot water soluble and methanol soluble substances (hereinafter referred to Fr. NaCl, Fr. HW and Fr. MeOH, respectively) were extracted from fruiting body of the mushroom. In vitro cytotoxicity tests, crude polysaccharides were not cytotoxic against cancer cell lines such as Sarcoma 180, HepG2, HT29 and NIH3T3 at the concentration of 2.0 mg/ml. Intraperitoneal injection with crude polysaccharides showed life prolongation effect of 23.4$\sim$37.2% in mice previously inoculated with Sarcoma 180. Fr. MeOH and Fr. HW exhibited the immuno-potentiating activity of B lymphocyte by increasing the alkaline phosphatase activity by 2.2$\sim$11.9 folds compared with control at the concentration of 0.2$\sim$0.5 mg/ml. In case of Fr. NaCl, the numbers of peritoneal exudate cells and circulating leukocytes were increased by 6.0 and 1.5 folds at the concentration of 50 mg/kg, respectively. Therefore, it is concluded that crude polysaccharides extracted from fruiting body of Tricholoma matsutake showed antitumor and immuno-potentiating activity against Sarcoma 180 of mouse.

• Korean Journal of Environmental Agriculture
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• v.37 no.1
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• pp.21-27
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• 2018

BACKGROUND: Although 1 M $NH_4OAc$ (pH 7.0) is predominantly used as the extractant of exchangeable cations in agricultural soils, this method is unsuitable for extracting the cations in saline and calcareous soils. This study was performed to select a proper method to determine exchangeable cations in vinyl greenhouse soils. METHODS AND RESULTS: Exchangeable cations (Ca, Mg, K, Na) in saline vinyl greenhouse soils were determined after extraction with 1 M $NH_4OAc$ (pH 7.0 and 8.5) and 1 M alcoholic $NH_4Cl$ (pH 8.5). Sum of exchangeable cations of the soils extracted with 1 M $NH_4OAc$ at pH 7.0 was 1.9-2.5 times greater than soil cation exchange capaity determined at pH 7.0, even though soluble salts were pre-removed. A similar result was found when the cations were extracted with 1 M $NH_4OAc$ at pH 8.5. Those results are mostly due to the overestimation of exchangeable Ca and Mg, linked to a partial dissolution of sparingly soluble salts in $NH_4OAc$ solution. When extracted with 1 M alcoholic $NH_4Cl$ at pH 8.5, extractable Ca and Mg decreased significantly due to the lower solubility of Ca and Mg carbonates in the extractant. And the sum of exchangeable cations was very close to the corresponding exchange capacity of soils. CONCLUSION: Alcoholic $NH_4Cl$ (pH 8.5) is proposed as a reliable extractant in determination of exchangeable cations in saline vinyl greenhouse soils. And soluble salts should be removed prior to the extraction of exchangeable cations.

• Journal of Bio-Environment Control
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• v.22 no.2
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• pp.162-166
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• 2013

The objective of this study was to investigate the influence of fertilization levels by soil testing on plant growth and fruit quality of musk melon in greenhouse cultivation. Leaf area and fruit weight were severely affected by fertilization level. Leaf areas were significantly reduced by 54.1% and 24.5% at 0 and 50% fertilization level compared to the 100% fertilization level, respectively. Fruit weights were reduced by 38.2% and 19.9% at 0 and 50% fertilization level compared to the 100% fertilization level, respectively. However, soluble solids and sucrose contents were increased by 1.8% and 23.3% at 50% fertilization level compared to the 100% fertilization level, respectively. These results suggest that reduction of 50% fertilization level by soil testing seem to be effective methods to reduce salt accumulation in the soil as well as increasing of fruit quality such as soluble solids and sucrose contents.

• Food Science of Animal Resources
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• v.25 no.1
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• pp.45-51
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• 2005

This study was carried out to get the informations on microbes and protein extractability through comparing the quality attributes of Hanwoo fed in Korea, Hanwoo fed in Japan and Japanese Wagyu. The fresh beefs were stored at 4±l℃ for 13 days. In microbiological test, the total plate counts were higher in rump than in other beef portion as loin, chuck (p<0.000l). The number of psychrotrobes in the rump were maintained high levels (p>0.0001) for storage period, whereas the loin from Hanwoo fed in Korea, Hanwoo fed in Japan and Wagyu were lowest levels. The number of E. coli were no significantly different among the samples. In lactic acid bacteria, the loin form 3 grade Hanwoo (K3) had highest levels (p<0.0001). Comparing to the protein extractability, water soluble proteins were high in chuck (p<0.001). In the case of loin, water soluble proteins of K3 (3 grade Hanwoo) and Wagyu were high as 3.010 mg/g and 2.977 mg/g, respectively (p<0.001). Salt soluble protein of K1 (1 grade Hanwoo) was high as 7.437 mg/g (p<0.0001).

• Applied Biological Chemistry
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• v.29 no.1
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• pp.51-56
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• 1986

Using milled barley of four varieties Olbori, Youngsan-bori, Sacheon 6, and Suwon 228, the barley proteins were fractionated by the Osborne method and the modified Osborne method. Two fractionation methods were compared. There was a steady increase in the amount of nitrogen extracted as NaCl concentration increased, reaching a maximum at 0.5M NaCl and the extraction of nitrogen by 0.5M NaCl reached a maximum at $22^{\circ}C$. Alcohol-soluble fraction was least extracted by 70% (v/v) ethanol at $4^{\circ}C$ and most by sequential extraction with 50% (v/v) propan-1-ol alone followed by 50% (v/v) propan-1-ol plus 3% (v/v) 2-mercaptoethanol. Nitrogen was least extracted between pH 4 and 6 and most extracted at higher pH than 10. The modified Osborne fractionation of the protein complex in the four barleys showed that the salt-soluble nitrogen accounted for 21,4% to 24.1%, hordein-I varied from 30.4% to 43.4%, hordein-II varied widely from 9.3% to 19.5% and borate buffer-soluble glutelin content ranged from 17.1% to 23.7%.

• The Korean Journal of Mycology
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• v.34 no.2
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• pp.105-111
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• 2006

Tremella fuciformis, one of edible and medicinal mushroom belonging to Tremellaceae of Basidiomycota, has been known to have a curative effect on sarcoma 180 of mice and lowering high blood pressure of human beings. Neutral salt soluble [0.9% NaCl (Fr. NaCl)], hot water soluble (Fr. HW) and methanol soluble (Fr. MeOH) substances were extracted from Tremella fuciformis. In vitro cytotoxicity tests, Fr. HW and Fr. NaCl were not cytotoxic against cancer cell lines such as NIH3T3, Sarcoma 180, and HT-29 at the concentration of $2000{\mu}g/ml$, while Fr. MeOH was cytotoxic to NIH3T3 and Sarcoma 180. Intraperitoneal injection with Fr. NaCl showed antitumor effect with life prolongation of 53% in mice inoculated with Sarcoma 180. Fr. NaCl improved the immunopotentiation activity of B lymphocyte by increasing the alkaline phosphatase activity by $3.0{\sim}8.1$ folds, respectively. Intraperitoneal injection with Fr. NaCl increased the numbers of peritoneal exudated cells and circulating leukocytes by 7.4 folds and 1.6 folds, respectively, than in the control group. The antitumor effect of T. fuciformis against Sarcoma 180 of mice was likely due to immunopotentiation activity.

• The Korean Journal of Mycology
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• v.34 no.2
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• pp.98-104
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• 2006

Armillaria mellea, one of edible and medicinal mushroom belonging to Tricholomataceae of Basid-iomycota, has been known to have outstanding inhibitive effects on the sarcoma 180 and Erhrlich carcinoma of mice. Neutral salt soluble (0.9% NaCl), hot water soluble and methanol soluble substances (hereinafter referred to Fr. NaCl, Fr, HW and Fr. MeOH, respectively) were extracted from the mushroom. In vitro cytotoxicity tests, crude polysaccharide were not cytotoxic against cancer cell lines such as NIH3T3 and Sarcoma 180 at the concentration of $1000{\mu}g/ml$. Intraperitoneal injection with crude polysaccharides exhibited life prolongation effect of $60{\sim}67.5%$ in mice inoculated with Sarcoma 180, respectively. Fr, NaCl improved the immunopotentiation activity of B lymphocyte by increasing the alkaline phosphatase activity by $1.8{\sim}3.0$ folds, respectively. In case of Fr. NaCl, the numbers of peritoneal exudate cells and circulating leukocytes were increased by 10 and 2 folds, respectively.