Browse > Article
http://dx.doi.org/10.5352/JLS.2008.18.9.1290

Antitumor and Immuno-potentiating Activity against Mouse Sarcoma 180 by Crude Polysaccharides Extracted from Fruiting Body of Tricholoma matsutake  

Hur, Hyun (Department of Life Science, Dongguk University)
Choi, Yon-Il (Department of Biology, University of Incheon)
Lee, Tae-Soo (Department of Biology, University of Incheon)
Publication Information
Journal of Life Science / v.18, no.9, 2008 , pp. 1290-1298 More about this Journal
Abstract
Tricholoma matsutake, one of edible and medicinal mushroom belonging to Tricholomaceae of Agaricales, has been known to contain some curing effect on gastric cancer and ulcer, and inhibitory effect on sarcoma 180 and Ehrlich sarcoma. Neutral salt soluble (0.9% NaCl), hot water soluble and methanol soluble substances (hereinafter referred to Fr. NaCl, Fr. HW and Fr. MeOH, respectively) were extracted from fruiting body of the mushroom. In vitro cytotoxicity tests, crude polysaccharides were not cytotoxic against cancer cell lines such as Sarcoma 180, HepG2, HT29 and NIH3T3 at the concentration of 2.0 mg/ml. Intraperitoneal injection with crude polysaccharides showed life prolongation effect of 23.4$\sim$37.2% in mice previously inoculated with Sarcoma 180. Fr. MeOH and Fr. HW exhibited the immuno-potentiating activity of B lymphocyte by increasing the alkaline phosphatase activity by 2.2$\sim$11.9 folds compared with control at the concentration of 0.2$\sim$0.5 mg/ml. In case of Fr. NaCl, the numbers of peritoneal exudate cells and circulating leukocytes were increased by 6.0 and 1.5 folds at the concentration of 50 mg/kg, respectively. Therefore, it is concluded that crude polysaccharides extracted from fruiting body of Tricholoma matsutake showed antitumor and immuno-potentiating activity against Sarcoma 180 of mouse.
Keywords
Antitumor effect; crude polysaccharides; immuno-potentiation; Tricholoma matsutake;
Citations & Related Records
Times Cited By KSCI : 9  (Citation Analysis)
연도 인용수 순위
1 Oh, Y. H., W. Y. Lee, M. W. Lee, M. J. Shim and T. S. Lee. 2004. Immuno-modulatory and antitumor effect of crude polysaccharides extracted from sclerotium of Grifola umbellata. The Korean Journal of Mycology 32, 23-30.   DOI   ScienceOn
2 Ying, J. Z., X. L. Mao, Q. M. Ma, Y. C. Zong and H. A. Wen. 1987. Icons of medicinal fungi from China. Science Press, Beijing, China.
3 Lee, K. W., H. Y. Kim, H. Hur, M. W. Lee, M. J. Shim, W. Y. Lee and T. S. Lee. 2008. Antitumor and immuno-modulatory effect of crude polysaccharides from fruiting body of Tremella aurantialba against mouse sarcoma 180. The Korean Journal of Mycology 36, 66-74.   과학기술학회마을   DOI   ScienceOn
4 Park, W. H. and H. D. Lee. 2002. Mushrooms in Korea. Kyo-Hak Publishing Co., Ltd., Seoul.
5 Lee, K. Y., M. J. Han, S. Y. Park and D. H. Kim. 2000. In vitro and in vivo antitumor activity of the fruit body of Phellinus linteus. Kor. J. Food Sci. Technol. 32, 477-480.   과학기술학회마을
6 Mossman, B. T. 1983. In vitro approaches for determining mechanisms of toxicity and carcinogenicity by asbestos in the gastrointestinal and respiratory tracts. Environ. Health Perspect 53, 155-161.   DOI
7 Oh, Y. H., S. B. Kim, K. W. Lee, H. Y. Kim, M. J. Shim, H. S. Rho, H. S. Lee, M. W. Lee, W. Y. Lee and T. S. Lee. 2006. The immuno-modulatory and antitumor effects of crude polysaccharides extracted from Tremella fuciformis. The Korean Journal of Mycology 34, 105-111.   과학기술학회마을   DOI   ScienceOn
8 Shim, S. M., K. H. Im, W. Y. Lee, J. W. Kim, M. J. Shim, M. W. Lee and T. S. Lee. 2003a. Studies on immune-modulatory and antitumor effects of crude polysaccharides extracted from Paecilomyces sincluirii. The Korean Journal of Mycology 31, 155-160.   DOI   ScienceOn
9 Shim, S. M., K. W. Im, J. W. Kim, W. Y. Lee, H. W. Kim, M. W. Lee and T. S. Lee. 2003b. The immuno-modulatory and antitumor effects of crude polysaccharides extracted from Daedaleopsis tricolor. The Korean Journal of Mycology 31, 161-167.   DOI   ScienceOn
10 Suzuki, M., T. Arika, T. Ameriya and M. Fujiwara. 1982. Cooperative role of T lymphocytes and macropages in antitumor activity of mice pretreated with schizophyllan. Jpn. J. Exp. Med. 50, 59-65.
11 Choi, Y. I., K. W. Lee, H. Hur, W. Y. Lee and T. S. Lee. 2008. Immuno-potentiating and Antitumor Effects against Mouse Sarcoma 180 by Crude Polysaccharides Extracted from Fruiting Body of Russula rosacea. The Korean Journal of Mycology 36, 84-92.   과학기술학회마을   DOI   ScienceOn
12 Jin, M. L. 1996. A Study on the Activation of Immune Cell and Transcription Factor of Pine Nut Mushroom. Collected Papers of Seoul National University Graduate School. pp. 1-121.
13 Denizot, F. and R. Lang. 1986. Rapid colorimetric assay for cell growth and survival modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J. Immunol. Methods 89, 271-277.   DOI   ScienceOn
14 Fukuda, K., T. Uematsu, A. Hamada and S. Akiya. 1975. The polysaccharide from Lampteromyces Japonicus. Chem. pharm. Bull. 23, 1955-1961.   DOI   ScienceOn
15 Ji, J. H. and M. N. Kim. 2000. Antimutagenic and cytotoxicity effects of Phellinue linteus extracts. J. Kor. Soc. Food Sci. Nutr. 29, 322-330.
16 Kim, S. B., K. W. Lee, H. Y. Kim, M. J. Shim, H. S. Rho, H. S. Lee, M. W. Lee, W. Y. Lee and T. S. Lee. 2006. Articles: Inhibitive effect of mouse sarcoma 180 by crude polysaccharide extracted from fruiting Body of Aramillaria mellea. The Korean Journal of Mycology 34, 98-104.   과학기술학회마을   DOI   ScienceOn
17 Kim S. B., K. W. Lee, W. Y. Lee and T. S. Lee. 2007. Articles: Studies on immuno-modulatory and antitumor effects of crude polysaccharides extracted from fruiting Body of Oudemansiella radicata, The Korean Journal of Mycology 35, 109-114.   과학기술학회마을   DOI   ScienceOn
18 Lee, K. W., H. Y. Kim, W. H. Lee and T. S. Lee, 2007. Antitumor and immune-modulatory effect against mouse sarcoma 180 of crude polysaccharides extracted from fruiting Body of Armillaria tabescens. The Korean Journal of Mycology 35, 101-108.   과학기술학회마을   DOI   ScienceOn
19 Arthur, C. and M. D. Guyton. 1986. Textbook of medical physiology. 7th Eds. W. B. Saunder Company. pp. 51-59.
20 Allison, D. C. and P. Ridolpho. 1980. Use of a trypan blue assay to measure the deoxyribonucleic acid content and radioactive labeling of viable celIs. J. Histochem. Cytochem. 28, 700-703.   DOI   ScienceOn
21 Cho, S. M., J. H. Lee, S. B. Han, H. M. Kim, S. H. Yoo and J. D. Yoo. 1995a. Immuno- stimulating Polysaccharides from the Fruiting Bodies of Fomitella fraxinea (1)Characterization of polysaccharides extracted with neutral sodium chloride solution. The Korean Journal of Mycology 23, 332-339.   과학기술학회마을
22 Cho, S. M., J. H. Lee, S. B. Han, H. M. Kim, S. H. Yoo and I. D. Yoo. 1995b. Immuno- stimulating Polysaccharides from the Fruiting Bodies of Fomitella fraxinea (2)- Isolation and characterization of hot- water extracted polysaccharides . The Korean Journal of Mycology 23, 340-347.   과학기술학회마을