Browse > Article
http://dx.doi.org/10.4489/KJM.2004.32.1.023

Immuno-modulatory and Antitumor Effect of Crude Polysaccharides Extracted from Sclerotium of Grifola umbellata  

Oh, Yun-Hee (Department of Biology, University of Incheon)
Lee, U-Youn (Department of Biology, University of Incheon)
Lee, Min-Woong (Department of Applied Biology, Dongguk University)
Shim, Mi-Ja (Department of Life Science, University of Seoul)
Lee, Tae-Soo (Department of Biology, University of Incheon)
Publication Information
The Korean Journal of Mycology / v.32, no.1, 2004 , pp. 23-30 More about this Journal
Abstract
Neutral salt-soluble, hot water-soluble and methanol-soluble materials (hereinafter referred to Fr. NaCl, Fr. HW and Fr. MeOH, respectively) were extracted from sclerotium of Grifola umbellata. The Fr. NaCl and Fr. HW did not show any direct cytotoxicity against NIH3T3, Sarcoma 180 and MCF-7, but Fr. MeOH showed cytotoxicity against these cell lines at the concentration of $1,000\;{\mu}g/ml$. Intraperitoneal injection with Fr. NaCl showed antitumor effect with life prolongation of 66.7% and decrease the number of Sarcoma 180 cells of 54.2% in mice inoculated with Sarcoma 180. Fr. NaCl improved the immunopotentiation activity through alternative complement pathway and the alkaline phosphatase activity by $85.05{\sim}88.73%$ and 6 folds, respectively. The number of peritoneal exudate cells and the circulating leucocytes were increased by 1.7 and 3.6 folds in the Fr. NaCl treating group compared with the control group, respectively. The weight of immunoorgans such as liver, spleen and thymus were also gradually increased. The hematological analysis of the Fr. NaCl group was similar with that of the control group. The total polysaccharide and protein contents of Fr. NaCl were 98.25% and 1.44%, respectively. These results indicate that the antitumor activity of Fr. NaCl was exerted through immunopotentiation, but not through cytotoxicity against the tumor cells.
Keywords
Antitumor acitivity; Grifola umbellata; Immuno-modulatory effect; Sarcoma 180;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Role of nutrition in immunologic function /
[ Gross, R.L.;Newberne, P.M. ] / Physiol. Rev.   DOI
2 Calvacine, a new antitmor agent /
[ Roland, J.F.;Chmielewicz, Z.F.;Weiner, B.A.;Gross, A.M.;Boenong, O.P.;Luck, J.V.;Bardos, T.J.;Rerlly, H.C.;Sugiura, K.;Stock, C.C.;Lucas, E.H.;Byerrum, R.U.;Stevens, J.A. ] / Science
3 Protein-bound polysaccaride preparation, PS-K, effective against mouse Sarcoma 180 and rat ascites hepatoma AH-13 by oral use /
[ Tsugagoshi, S.;Ohashi, F. ] / Gann.
4 Host-mediated antitumor action of schizophyllan, a glucan produced by Schizophyllum commune /
[ Komatsu, N.;Okubo, S.;Kikumoto, S.;Kimura, K.;Saito, G. ] / Gann.
5 Induction of alkaline phosphatase activity in murine spleen cells treated with various mitogens /
[ Ohno, N.;Arai, Y.;Suzuki, I.;Yadomae, T. ] / J. Phamacobio-Dyn.   DOI
6 Isolation and characterization of new antitumor polysaccharide, KS-2, extracted from culture mycelia of Lentinus edodes /
[ Fujii, T.;Maeda, H.;Suzuki, F.;Ishida, N. ] / J. Antibio.   DOI
7 Alkaline phosphatase activity as a membrane marker for activated B cells /
[ Garcia-Rozas, C.;Plaza, A.;Diaz-Espada, F.;Kreisler, M.;Martinez-Alonso, C. ] / J. Immunol.
8 Chemical analysis of microbial cells /
[ Herbert, D.;Phipps, P.J.;Strange, R.E. ] / Methods in Microbiology
9 Alkaline phosphatase activity is expressed only in B lymphocytes committed to proliferation /
[ Kasyapa, C.S.;Ramanadham, M. ] / Immunol Lett.   DOI   ScienceOn
10 Studies on the antineoplastic components of Korean Basidiomycetes /
[ Kim, B.K.;Chung, H.S.;Chung, K.S.;Yang, M.S. ] / Kor. J. Mycol.
11 Isolation of antitumor compounds from Paecilomyces tenuipes and induction of apoptosis on human cancer cell lines /
[ Kim, H.W.;Lindequist, U.;Kim, Y.H. ] / Proceedings of the fifth Korea-China joint symposium for mycology
12 Macrophage activation in vitro by chemically crosslinked <TEX>$(1{\to}3)-{\beta}D-glucan$</TEX> /
[ Adachi, Y.;Ohno, N.;Ohsawa, M.;Oikawa, S.;Yadomae, T. ] / Chem. Pharm. Bull.   DOI   ScienceOn
13 Textbook of medical physiology /
[ Arthur, C.;Guyton, M.D. ] /
14 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding /
[ Bradford, M.M. ] / Anal. Biochem.   DOI   ScienceOn
15 Inhibition of mouse Sarcoma 180 by polysaccharides from Lentinus edodes (Berk.) /
[ Chihara, G.;Maeda, Y.;Hamuro, J.;Sasaki, T.;Fukuoka, F. ] / Sing. Nature
16 Rapid colorimetric assay for cell growth and survival modifications to the tetrazolium dye procedure giving improved sensitivity and reliability /
[ Denizot, F.;Lang, R. ] / J. Immunol. Methods   DOI   ScienceOn
17 삼색도장버섯(Daedaleopsis tricolor)에서 추출한 조다당류의 면역 활성 및 항암 효과 /
[ 심성미;임경환;김정완;이우윤;김하원;이민웅;이태수 ] / 한국균학회지   DOI   ScienceOn
18 Fomitella fraxinea로부터 분리한 면역활성 다당류(I)-중성염 용액 추출다당류의 특성 /
[ 조수묵;이재훈;한상배;김환묵;유승헌;유익동 ] / 한국균학회지
19 Fomitella fraxinea로부터 분리한 면역활성 다당류(II)-열수추출 다당류의 분리 및 특성 /
[ 조수묵;이재훈;한상배;김환묵;유승헌;유익동 ] / 한국균학회지
20 까치버섯(Polyozellus multiplex) 추출물의 항산화 및 항암 효과 /
[ 한정;이인선 ] / 한국균학회지
21 キノコの化學.生物學, 學會出版センタ /
[ 水野 卓;川合正允 ] /
22 원형질체 융합 및 핵전이에 의한 새로운 담자균류의 개발에 관한 연구(II)-융합균사체의 항암성분이 생쥐의 면역세포에 미치는 영향 /
[ 김병각;문철;김채균;윤종명;심미자;김하원;최응칠 ] / 생약학회지
23 한국 약용버섯 도감 /
[ 박완희;이호득 ] /
24 매미눈꽃동충하초(Paecilomyces sinclairii)로부터 추출한 조다당류의 면역 활성과 항암 효과에 관한 연구 /
[ 심성미;임경환;이우윤;김정완;심미자;이민웅;이태수 ] / 한국균학회지   DOI