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http://dx.doi.org/10.4489/KJM.2006.34.2.098

Inhibitive Effect of Mouse Sarcoma 180 by Crude Polysaccharide Extracted from Fruiting Body of Aramillaria mellea  

Kim, Sang-Beom (Department of Biology, University of Incheon)
Lee, Gun-Woo (Department of Biology, University of Incheon)
Kim, Hye-Young (Department of Biology, University of Incheon)
Shim, Mi-Ja (Department of Life Science, University of Seoul)
Rho, Hyun-Su (Department of Microbiology, Gyeongsang National University)
Lee, Hyun-Sook (Department of Microbiology, Gyeongsang National University)
Lee, Min-Woong (Department of Biology, Dongguk University)
Lee, U-Youn (Department of Biology, University of Incheon)
Lee, Tae-Soo (Department of Biology, University of Incheon)
Publication Information
The Korean Journal of Mycology / v.34, no.2, 2006 , pp. 98-104 More about this Journal
Abstract
Armillaria mellea, one of edible and medicinal mushroom belonging to Tricholomataceae of Basid-iomycota, has been known to have outstanding inhibitive effects on the sarcoma 180 and Erhrlich carcinoma of mice. Neutral salt soluble (0.9% NaCl), hot water soluble and methanol soluble substances (hereinafter referred to Fr. NaCl, Fr, HW and Fr. MeOH, respectively) were extracted from the mushroom. In vitro cytotoxicity tests, crude polysaccharide were not cytotoxic against cancer cell lines such as NIH3T3 and Sarcoma 180 at the concentration of $1000{\mu}g/ml$. Intraperitoneal injection with crude polysaccharides exhibited life prolongation effect of $60{\sim}67.5%$ in mice inoculated with Sarcoma 180, respectively. Fr, NaCl improved the immunopotentiation activity of B lymphocyte by increasing the alkaline phosphatase activity by $1.8{\sim}3.0$ folds, respectively. In case of Fr. NaCl, the numbers of peritoneal exudate cells and circulating leukocytes were increased by 10 and 2 folds, respectively.
Keywords
Antitumor effect; Armillaria mellea; Crude polysaccharides; Immuno-modulatory;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 구현옥, 김인천, 박신자, 정상희, 박종명, 이재진, 유한상, 이영순. 1999. 신령버섯(Agaricus blzei Murill)에서 분리한 다당체의 Staphylococcus aureus 감염 및 마우스 Sarcoma 180 종양세포에 대한 방어효과. 한국실험동물학회지 15: 155-158
2 박상신, 유국현, 민태진. 1998. 버섯추출물의 항산화 활성에 관한 연구. 한국균학회지 26: 69-77
3 박완희, 이호득. 1999. 한국약용버섯도감, 교학사
4 심성미, 임경환, 이우윤, 김정완, 심미자, 이민웅, 이태수. 2003a. 매미눈꽃동충하초(Paecilomyces sinclairii)에서 추출한 조다당류의 면역활성 및 항암효과에 관한 연구. 한국균학회지 31: 155-160   DOI
5 심성미, 임경환, 김정완, 이우윤, 김하원, 이민웅, 이태수. 2003b 삼색도장버섯(Daedaleopsis tricolor)에서 추출한 조다당류의 면역활성 및 항암효과. 한국균학회지 31: 161-167   DOI
6 신혜원, 김하원, 최응칠, 도상학, 김병각. 1985. 한국산 영지의 무기 성분 및 면역 증강 작용에 관한 연구. 생약학회지 16: 181-190
7 오윤희, 이우윤, 이민웅, 심미자, 이태수. 2004. 저령(Grifola umbellata)의 균핵에서 추출한 조다당류의 면역활성 및 항암 효과. 한국균학회지 32: 23-30   DOI
8 조수묵, 이재훈, 한상배, 김환묵, 유승헌, 유익동. 1995. Fomitella fraxinea로부터 분리한 면역활성 다당류(I)-중성염 용액 추출 다당류의 특성. 한국균학회지 23: 332-339
9 조수묵, 이재훈, 한상배, 김환묵, 유승헌, 유익동. 1995. Fomitella fraxinea로부터 분리한 면역활성 다당류(II)-열수추출 다당류의 분리 및 특성. 한국균학회지 23: 340-347
10 Choi, S. H., Jun, C. D., Lee, B. S., Park, S. D., Oh, J. S. and Chung, H. T. 1993. Effect of various extrinsic and intrinsic factors on the nitric oxide production of murine macrophage. Kor. J. BRM 3: 15-22
11 Denizot, F. and Lang, R. 1986. Rapid colorimetric assay for cell growth and survival modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J. lmmunol. Methods 89: 271-277   DOI   ScienceOn
12 Gross, R. L. and Newberne, P. M. 1980. Role of nutrition in immunologic function. Physiol. Rev. 60: 188-302   DOI
13 Jan, A. M., Marielle, E. B., Peter, H. N., Pieter, S. H. and Ralph, V. F. 1992. INF-${\gamma}$ induced 1-arginine-dependent toxoplasmastic activity in murine peritoneal macrophages is mediated by endogenous tumor necrosis factor-${\alpha}$. J. lmmunol. 148: 568-571
14 Kishida, E., Sone, Y., Shibata, S. and Misaki, A. 1981. Preparation and immunochemical characterization of antibody of branched ${\beta}-(1{\rightarrow}3)-D-glucan$of Volvariella volvacea and its use in studies of antitumor actions. Agric. Biol. Chem. 53: 1849-1859
15 Komatsu, N., Okubo, S., Kikumoto, S., Kimura, K. and Saito, G 1969. Host-mediated antitumor action of schizophyllan, a glucan produced by Schizophyllum commune. Gann. 60: 137-44
16 Mossman, B. T. 1983. In vitro approaches for determining mechanisms of toxicity and carcinogenicity by asbestos in the gastrointestinal and respiratory tracts. Environ. Health Perspect. 53: 155-161   DOI
17 Tsugagoshi, S. and Ohashi, F. 1974. Protein-bound polysaccaride preparation, PS-K, effective against mouse sarcoma 180 and rat ascites hepatoma AH-13 by oral use. Gann. 65: 557
18 한만덕, 이은숙, 김영권, 이중우, 정훈, 윤경하. 1998. 영지의 균사체성 ${\beta}$-glucan에 의한 RAW 264.7 대식세포의 Nitric Oxide 생성 . 한국균학회지 26: 246-255
19 Suzuki, M., Arika, T., Amemiya, T. and Fujiwara, M. 1982. Cooperative role of T lymphocytes and macrophages in antitumor activity of mice pretreated with schizophyllan. Jpn. J Exp. Med. 50: 59-65
20 Chihara, G., Maeda, Y., Hamuro, J., Sasaki, T. and Fukuoka, F. 1969. Inhibition of mouse Sarcoma 180 by polysaccharides from Lentinus edodes (Berk.) Sing. Nature 222: 687-688   DOI   ScienceOn
21 Whistler, R. L., Bushway, A. A., Singh, P. P., Nakahara, W. and Tokuzen, R. 1976. Noncytotoxic, antitumor polysaccharides. Pp 235-275. In: Tipson, R. S. and Horton, D. Eds. Advances in Carbohydrate Chemistry and Biochemistry. vol. 32. Academic Press, New York
22 진미림. 1996. 잣버섯 성분의 면역세포 및 전사인자의 활성화 작용에 관한 연구. 서울대학교 대학원 논문집. Pp 1-121
23 水野 卓, 川合正允. 1992. キノコの化學 . 生物學. 學會出版センタ
24 Arthur, C. and Guyton, M. D. 1986. Textbook of Medical Physiology. 7th Ed. W. B. Saunder company. Pp 51-59
25 Chihara, G., Hamuro, G., Meada, Y., Arai, Y. and Fukoka, F. 1970. Antitumor polysaccharide derived chemically from natural glucan (Pachman). Nature 225: 973-948
26 Ohno, N., Arai, Y., Suzuki, I. and Yadomae, T. 1986. Induction of alkaline phosphatase activity in murine spleen cells treated with various mitogens. J Phamacobio-Dyn. 9: 593-599   DOI
27 Roland, J. F., Chmielewicz, Z. F., Weiner, B. A., Gross, A. M., Boenong, O. P., Luck, J. V., Bardos, T. J., Redly, H. C., Sugiura, K., Stock, C. C., Lucas, E. H., Byerrum, R. U. and Stevens, J. A. 1960. Calvacine, a new antitumor agent. Science 132: 1987
28 Sugihara, T., Yoshioka, Y. and Nishioka, K. 1972. Anticomplementary activity of antitumor polysacchrides. Nature New Biology 235: 59-60