• Journal of Physiology & Pathology in Korean Medicine
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• v.21 no.3
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• pp.605-610
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• 2007

This experimental study was performed to evaluate the effects of ehtanol extract of Purple Solanum tuberosum L(EPSTL) on obesity and hyperlipidemia in rats induced by high fat diet. Sprague-Dawley rats were divided into normal group, control group, EPSTL (100 mg/kg and 200 mg/kg) treated group. EPSTL was orally administered to the obese rats by high fat diet for 6 weeks. The effect of EPSTL on obesity and hyperlipidemia were examined in vitro and in vivo as follows : EPSTL effectively inhibited the differentiation of 3T3-L1 adipocytes in a concentration dependent manner. EPSTL significantly lowered body weight of rats fe high fat diet for 6 weeks from 4 weeks treatment compared with untreated control. EPSTL significantly reduced the weight of retroperitoneal fat only at 100 mg/kg and epididymal fat pad in rats fed high fat diet for 4 weeks at 100 and 200 mg/kg. EPSTL significantly reduced the level of triglyceride at 200 mg/kg, while EPSTL tended to lower the levels of total lipid and phospholipid in rats fed high fat diet for 4 weeks with no significance. EPSTL significantly attenuated the level of total cholesterol, LDL and atherosclerosis index (Al) as well as increased the level of HDL at 200 mg/kg. EPSTL significantly reduced the levels of total lipid and triglyceride of hepatic tissues in rats fed high fat diet for 4 weeks at 200 mg/kg. EPSTL significantly lowered the level of insulin, while it did not affect leptin compared with untreated control. Taken together, these results suggest that EPSTL can be used for the treatment or prevention of hyperlipidemia and obesity.

• Journal of Bio-Environment Control
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• v.15 no.4
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• pp.340-345
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• 2006

A potato (Solanum tuberosum L. cv. Superior) cultivar was grown in aeroponic cultivation system to investigate the effect of root zone cooling in summer. Based on their nutrient uptake, growth responses, and tuberization, the possibilities for potato seed production were determined. Although shoot growth and early tuberization increased in the conventional non-cooling root zone system (root zone temperature of $25\pm2^{\circ}C$), stolen growth, photosynthesis, transpiration rate and number of tubers produced were higher in the cooling root zone system ($20\pm2^{\circ}C$) than in the non-cooling system. Increasing root zone temperature above $25^{\circ}C$ stimulated absorption of K more than T-N, P, Ca, Fe and Mn. On the other hand, root zone temperatures in the range of $20^{\circ}C$ to $25^{\circ}C$ did not affect Mg contents. The lower uptake and supply to leaves of T-N, Fe and Mn at the high root zone temperature promoted early tuberization and advanced haulm senescence. The results stress the importance of keeping root zone temperature to as low as below 20, particularly in summer under temperate Bone.

• Korean Journal of Plant Resources
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• v.10 no.4
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• pp.300-304
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• 1997

Leaf dise explants of Solanum tuberosum cultivar. Desiree and Atlantic, were infected with a Agrobacterium MP90 strain containing chimeric gene construct, consisting of antibiotic and low temperature regulated gene (H28) for transformation. regenerated multiple shoots were selected on a medium containing kanamycin and carbenieillin after exposure to Agrobacterium. Both PCR analysis of NPT Ⅱ, H28 genes and northern blot analysis indicated that the genes coding for the enzyme were successfully integrated into the potato genome and could be expressed in potato plants.

• Plant Biotechnology Reports
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• v.2 no.1
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• pp.13-20
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• 2008

Potato (Solanum tuberosum L.), one of the most important food crops, is susceptible to a number of devastating fungal pathogens in addition to bacterial and other pathogens. Producing disease-resistant cultivars has been an effective and useful strategy to combat the attack of pathogens. Potato was transformed with Agrobacterium tumefaciens strain EHA101 harboring chitinase, (ChiC) isolated from Streptomyces griseus strain HUT 6037 and bialaphos resistance (bar) genes in a binary plasmid vector, pEKH1. Polymerase chain reaction (PCR) analysis revealed that the ChiC and bar genes are integrated into the genome of transgenic plants. Different insertion sites of the transgenes (one to six sites for ChiC and three to seven for bar) were indicated by Southern blot analysis of genomic DNA from the transgenic plants. Expression of the ChiC gene at the messenger RNA (mRNA) level was confirmed by Northern blot analysis and that of the bar gene by herbicide resistance assay. The results obviously confirmed that the ChiC and bar genes are successfully integrated and expressed into the genome, resulting in the production of bialaphos-resistant transgenic plants. Disease-resistance assay of the in vitro and greenhouse-grown transgenic plants demonstrated enhanced resistance against the fungal pathogen Alternaria solani (causal agent of early blight).

• KSBB Journal
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• v.6 no.1
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• pp.27-33
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• 1991

Hairy root induced by A. rhizogenes from potato tuber (Solanm tuberosum L.) synthesized the agropine and mannopine which were demonstrated with paper electrophoresis. And the starch contents in hairy root were increased gradually following the developmental stage. But protein contents were decreased. The activity of ${\beta}-glucan$ synthetase II(GSII) which is related to the cell wall biosynthesis was stimulated in hairy root on the developmental stage. And chloropromazine did not influence the activity of GS II while verapamil inhibited about 60% of the activity GS II. Therefore, these results showed $Ca^{2+}$ to be effective factor in the cell wall formation. Isozyme pattern of peroxidase was investigated in the callus and hairy root induced from potato tuber.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.1
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• pp.133-137
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• 2001

The antioxidant activitiesof methanol extracts of twenty one vegetables were tested using the method of 1,1-diphenyl-2-picryl hydrazyl (DPPH) reactivity and TBARS substance assay in vitro. The methanol extracts of the leaves from three plants such as Aster scaber, Chrysanthemum coronarium, Petasites japonicus were found to be th most effective on DPPH radical scavenging activity. The next effective ones were the leaves of Capsicum annuum, the peels of Ipomoea batatas and Solanum tuberosum, the stems and leaves of Ipomoea batatas, the levels of Solanum melongena, the fruits of Solanum melongena, the green fruits (seed) of Capsicum ammuum in order, and the others did not show a considerable activity. When tested on lipid peroxidation using a lipid peroxidation generation system mediated by $H_{2}O_{2}/Fe^{2+}$ in rat liver homogenates. the peels of Cucumis melo, the leaves and the fruits of Solanum melongena, of Solanum melongena, The levels of Aster scaber and Chrysanthemum coronarium, the stems and leaves of Ipomoea batatas and the peels of Ipomoea batatas were found to exhibit a good protective activity against lipid peroxidatian in the order of effectiveness.

• Korean Journal of Environmental Agriculture
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• v.35 no.3
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• pp.202-210
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• 2016

BACKGROUND: This study was conducted to determine effects of ridging systems and plastic film mulch types on growth, yield, and profitability of potato (Solanum tuberosum L.) in spring cropping using paddy field and to suggest profitable ridging system and plastic mulch type.METHODS AND RESULTS: Two potato cultivars ('Goun', and 'Atlantic') were grown at paddy field located in Gangneung in spring. For treatments, ridging systems were one-row ridge (OR) and two-row ridge (TR). Mulch types were no-mulch (NM), black plastic film mulch (BPM), and transparent plastic film mulch (TPM). Emergence of sprout was affected by the mulch types and the fastest level was shown at TPM treatment. Accumulative soil temperature during sprouting was 16.2℃ higher at TR than at OR and also higher at TPM than at BPM or NM. Stem length was not affected by ridging systems or mulch types. Leaf area index (LAI) was influenced by mulch types, while not by ridging systems. The highest LAI was shown at TR with TPM and OR of BPM. There was no significant difference in specific gravity or dry matter rate by ridging systems and mulch types. Tuber yield was significantly influenced by ridging systems and mulch types. Mean tuber weight was heavier at OR treatment than at TR and also the heaviest at TPM among mulch types. The highest marketable yield was found at OR with BPM. Based on marketable tuber yield and market price, the highest income ratio in two cultivars was found at OR with BPM and it was 20~82% higher than the ratio at TR with TPM.CONCLUSION: In spring potato cropping using paddy field, OR with BPM is better for high yield and is more profitable for farmer's income than the conventional cultivation method, TR with TPM.

• Journal of Plant Biotechnology
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• v.42 no.2
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• pp.129-134
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• 2015

In order to compare salt tolerance among potato cultivars (Solanum tuberosum L.), in vitro plants of each cultivar were cultured on the Murashige and Skoog's medium containing different levels of NaCl (0, 75, 150, and 225 mM). The suitable level of NaCl for a comparison of the shoot and root growth between potato cultivars was 75mM. Shoot length and weight were better in 'Dejima', 'Superior', 'Jayoung', and 'Haryeong' than in other cultivars such as 'Goun' and 'Atlantic'. Normal root growth was observed in 'Seohong', 'Superior', and 'Haryeong', while 'Goun', 'Atlantic', 'Dejima', 'Jowon', and 'Chuback' showed no root. Proline contents in all the cultivars increased as affected by NaCl levels, then the increase amount was fewer in the cultivars showed a better growth in both shoot and root than in others showed a poor growth. As a result of this study, 'Superior' and 'Haryeong' were determined as salt tolerant cultivars and therefore using these cultivars it would be possible to potato production at salt arable land such as reclaimed tidal.

• Korean Journal of Environmental Agriculture
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• v.20 no.2
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• pp.116-121
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• 2001

To observe the stimulating effects of low dose ${\gamma}$ - radiation on the dormancy breaking and physiological activity, potato (Solanum tuberosum L. cv. Dejima) were irradiated at the dose of $0.5{\sim}30$ Gy. Low dose ${\gamma}$ - radiation exhibited promoting effects on the sprouting rate within the range of optimum dose of 1 Gy and 2 Gy and on the sprout length within the range of optimum dose of 2 Gy and 4 Gy. Regardless of storage duration and irradiation doses, the number of sprouts was enhanced. The number of sprouts, which were reserved for 15 days after production (DAP), were significantly increased under 4, 8 and 16 Gy irradiation. The growth of 45 DAP sprouts was extremely stimulated under 4 Gy irradiation accompanying the increase of peroxidase activity in the plantlet. In this study, it was also suggested that the activities of antioxidative enzymes of potato plantlets were not related to the irradiation dose during the plantlet development with the exception of decrease in catalase activity.

• Journal of Bio-Environment Control
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• v.15 no.2
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• pp.204-210
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• 2006

The purple color in flesh of 'Jasim' potato (Solanum tuberosum L.) tubers is due to an accumulation of anthocyanin. The experiment was conducted to determine what is the key factor among cultural environments that affect the accumulation of anthocyanin in 'Jasim' potato tubers, and the effect of nitrogen fertilization levels on that. Through analysis of anthocyanin content of purple-fleshed tubers produced under the different cultural environments of three localities and two planting dates, we determined that lower temperature during 30days before harvesting resulted in higher anthocyanin accumulation of 'Jasim' potato tubers. In the experiment of different level of nitrogen fertilization, 'Jasim' potato tubers produced in highland (Daegualryeong) showed higher content of anthocyanin in the treatment of recommended level than in treatment of lower or higher level as compared with the recommended level. However, significant difference of anthocyanin content among different nitrogen fertilization levels was not observed in lowland (Gangnung). These results suggest that the selection of locality or cropping system and the optimal fertilization management are very important factors for production of higher pigmented tubers in purple-fleshed potato.