• Title/Summary/Keyword: Solanum species

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Identification of Verticillium tenerum Isolated from Potato and Pumpkin in Korea (감자와 단호박에서 분리한 Verticillium tenerum의 동정)

  • Hyun, Ik-Hwa;Heo, Noh-Yeoul;Ryu, Kyoung-Yul;Kim, Jong-Tae;Chang, Seo-Yeon
    • The Korean Journal of Mycology
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    • v.32 no.1
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    • pp.45-49
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    • 2004
  • Verticillium tenerum was detected from the samples of potato (Solanum tuberosum L.) and pumpkin (Cucurbita maxima Duch.) in Korea. The samples on which this species was detected were potato tubers collected from Pyungchang and Gimjae, and fruits and seeds of pumpkin from Yeonchon in 2003. The conidiophores of the fungus produced mainly three to five phialides in verticil. The phialides were narrow, flask-shaped, only very slightly swollen at the base, and taper subulately to a narrow neck. They were hyaline, $10{\sim}26{\times}2{\sim}5\;{\mu}m$. The conidia were formed in slimy heads, and were oval to ellipsoidal, pale reddish-brown, $2.5{\sim}7.5{\times}2{\sim}4\;{\mu}m$. The colony on the substrata was unique, showing brick-red color. This is the first report on the distribution of Verticillium tenerum in Korea.

EFFECTS OF GAS EXHAUSTED FROM GASOLINE ENGINE ON PLANTS GROWN IN THE GREENHOUSE

  • Sugimoto, H.;Yamashita, J.
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 1993.10a
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    • pp.487-494
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    • 1993
  • In order to establish a fully automatized pest control in the a greenhouse , the authors developed a prototype of microcomputer installed spraying vehicle which traveled along the furrows. Since a power sprayer mounted on the vehicle was driven by gasoline engine, plants grown in the greenhouse might be injured by the gas exhausted from the engine. Thus , effects of exhausted gas on photosynthetic rate and the shedding of flowers and buds of plants were examined. At first, effects of exhausted gas on photosynthetic rate of potted sweet pepper (Capsicum annuum L.) and eggplant(Solanum melongena L.) plants were examined. In a closed vinyl house the engine was operated for 5 minutes and plants were exposed to the gas for 2hours in the daytime on a fine day. Photosynthetic rate did not significantly decreased by the treatment in both species. Secondly, effects of ehtylene on the shedding of flowers and buds of sesame (Sesamum indicum L. ) were examined. In the closed and partiall opened vinyl house, the engine was operated for 5 minutes and potted sesame plants were exposed to the gas for 12 hours in the night. In partially opened vinyl house, ethylene concentration decreased to 0 ppm 3 hours after the engine was stopped and flower and bud did not shed. In contrast, when vinyl house was closed ethylene concentration was 0.75 pm even 12 hours after the engine was stopped and flowers and buds shed markedly and epinasty was observed in upper young leaves. As mentioned above , it was revealed that injury of plants in the greenhouse caused by the gas exhausted from a gasoline engine could be prevented by providing suitable ventilation.

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First Report of Potato Stem-End Rot Caused by Fusarium oxysporum in Korea

  • Aktaruzzaman, Md.;Xu, Sheng-Jun;Kim, Joon-Young;Woo, Jae-Hyoun;Hahm, Young-Il;Kim, Byung-Sup
    • Mycobiology
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    • v.42 no.2
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    • pp.206-209
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    • 2014
  • In this study, we identified the causative agent of stem-end rot in potatoes that were grown in Gangwon alpine areas of Korea in 2013. The disease symptoms included appearance of slightly sunken circular lesion with corky rot on the potato surface at the stem-end portion. The fungal species isolated from the infected potatoes were grown on potato dextrose agar and produced white aerial mycelia with dark violet pigments. The conidiophores were branched and monophialidic. The microconidia had ellipsoidal to cylindrical shapes and ranged from $2.6{\sim}11.4{\times}1.9{\sim}3.5{\mu}m$ in size. The macroconidia ranged from $12.7{\sim}24.7{\times}2.7{\sim}3.6{\mu}m$ in size and had slightly curved or fusiform shape with 2 to 5 septate. Chlamydospores ranged from $6.1{\sim}8.1{\times}5.7{\sim}8.3{\mu}m$ in size and were present singly or in pairs. The causal agent of potato stem-end rot was identified as Fusarium oxysporum by morphological characterization and by sequencing the internal transcribed spacer (ITS1 and ITS4) regions of rRNA. Artificial inoculation of the pathogen resulted in development of disease symptoms and the re-isolated pathogen showed characteristics of F. oxysporum. To the best of our knowledge, this is the first study to report that potato stem-end rot is caused by F. oxysporum in Korea.

Geographical Distribution and Host plants of Corythucha marmorata (Uhler) (Hemiptera: Tingidae) in Korea (해바라기방패벌레(Corythucha marmorata (Uhler)) (Hemiptera: Tingidae)의 발생지역 및 기주식물)

  • Kim, Dong Eon;Kil, Jihyon
    • Korean journal of applied entomology
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    • v.53 no.2
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    • pp.185-191
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    • 2014
  • Corythucha marmorata (Uhler) belonging to the family Tingidae (Hemiptera) has been reported in Korea. In 2013, surveys were conducted to study its morphological characteristics, geographical distribution, and host plants. The adult is brown white and 2.77-3.28 mm in length, and the mature nymph is yellowish brown. Heavily infested trees appear yellowish white because they are fed on by the nymphs on the under surface of host plants. C. marmorata were found between early June and late October, and their numbers peaked in early July to late August. In 2013, the occurrence of C. marmorata was confirmed in 27 cities and counties nationwide, and 8 families and 24 species of host plants were identified. C. marmorata feeds on the following major host plants: Conyza canadensis, Aster pilosus, Helianthus annuus, Artemisia princeps, Rudbeckia bicolor, and Solanum melongena.

Characterization of Cucumber mosaic virus Isolated from Trifolium repens in Korea (국내 토끼풀에서 분리한 Cucumber mosaic virus의 특성)

  • Park, Tae Seon;Choi, Gug Seoun;Hong, Jin Sung
    • Research in Plant Disease
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    • v.22 no.1
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    • pp.55-58
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    • 2016
  • A Cucumber mosaic virus (named CMV-Tr1) isolated from the white clover (Trifolium repens) showing mosaic and malformation that found in a pepper field. Cucumber mosaic virus was identified through confirmation with PT-PCR, PCR-restriction fragment length polymorphism, and sequence analysis of coat protein (CP) gene. CMV-Tr1 mosaic symptom on the upper leaves of five tobacco species including Nicotiana benthamiana, Cucumis sativus, Physalis angulata, and Solanum lycopersicon. In Chenopodium quinoa and Vigna unguiculata the isolate showed local lesions in inoculated leaves. CMV-Tr1 compared with CMV-As in the sequence identity of CP gene. CMV-Tr1 showed 98.9% and 99.5% homologies at nucleotide and amino acid levels, respectively. Phylogenetic analysis of the CP gene indicated that CMV-Tr1 belongs to the CMV subgroup IB base on the CP. To our knowledge, this is the first report of CMV in T. repens in Korea.

Genetic diversity of conserved potato germplasm using microsatellite markers

  • Lee, Gi-An;Cho, Kwang-Soo;Shin, Myoung-Jae;Lee, Jung-Ro;Cho, Yang-Hee;Ma, Kyung-Ho
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.85-85
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    • 2017
  • Potato is important carbohydrate source over the world in that revealing high productivity per the unit area, and their cultivation area is estimated to be increased to cope with a scarcity of food according to the population increase. Major cultivated species of potato is Solanum tuberosum (2n = 4x = 48) and regarded as being originated in Andes region of South America. The diverse potato genetic resources has been collected and perserved in Highland Agricultural Research Institute (NICS, RDA), and the genetic materials as DNA stock is conserved in National Agrobiodiversity Center(NAS, RDA). The understanding of genetic constitution of conserved diversity is the basis for the germplam management and further utilization. In this study, we analyzed the genetic diversity of potato germplasm(479 accessions) using 24 microsatellite markers which have been internationally used for fingerprinting of potato accession. The allele number and polymorphic information content (PIC) of total accessions per locus was ranged from 2 to 18 (mean = 8.2) and from 0.214 to 0.771 (mean = 0.595), respectively. Especially, the accession originated from Korea revealed average allele number of 6.0 (2 - 11) and average PIC value of 0.58 (0.193 - 0.763). Three groups were deduced by phylogenic analysis (Group-1, -2, -3); Korean accessions showed close genetic similarity to Japanese and USA accessions, and Korean landraces were mainly included in Group-3. We try to elaborate the genetic diversity analysis of conserved potato germplasm by acquiring more genotypes using applicable molecular markers.

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Variation of Potato virus Y Isolated from Potato, Tobacco, Pea and Weeds in Korea on the C-terminal Region of Coat Protein Gene and 3'Non-translated Region

  • Yun, W.S.;Jung, H.W.;Oh, M.H.;Hahm, Y.I.;Kim, K.H.
    • The Plant Pathology Journal
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    • v.18 no.3
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    • pp.130-137
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    • 2002
  • Potato virus Y (PVY) is one of the most important viruses in many field crops in Korea. In this study, 31 PVY isolates were isolated from infected potato (Solanum tuberosum), tobacco (Nicotiana tabacum), pea (Pisum sativum), and weeds (Veronica persica, Lamium amplexicause and Capsella bursa-pastoris) showing different mosaic symptoms in Jeonbuk, Chungnam, Gangwon, and Gyeongbuk areas in Korea. The 640 nucleotide region containing the C-terminal portion of coat protein (CP) gene and 3'non-translated region (NTR) was amplified by reverse transcription-polymerase chain reaction (RT-PCR) using PVY-specific oligonucleotide primers. Sequence analyses of the amplified DNA fragments showed that the C-terminal portion of CP gene was not significantly different from that of previously reported PVY strains from potato (PVY-OK and -T) and tobacco (PVY-VN) in Korea. Homologies of the deduced CP amino acid sequences were 93.3-99.0% to corresponding regions of the other PVY strains including PV $Y^{N}$, PV $Y^{o}$ , PV $Y^{OK}$ , PV $Y^{T}$ , and PV $Y^{VN}$ . In contrast the sequences located at the 3'-NTR showed more diverse sequence homologies (76.4-99.7%). These results indicate that the C-terminal portion of the CP gene was relatively conserved while sequences at the 3'NTR were more diverse and variable over the host species and the regions where they were isolated.e isolated.

Screening of Korean Medicinal and Food Plants with Antioxidant Activity (한국 약용 및 식용식물들의 항산화성 식물탐색)

  • Chung, Il-Min;Kim, Kwang-Ho;Ahn, Joung-Kuk
    • Korean Journal of Medicinal Crop Science
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    • v.6 no.4
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    • pp.311-322
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    • 1998
  • Sixty medicinal and food plants native to Korea were mainly selected with old traditional habit and antioxidant activity was investigated. The 80% EtOH extracts of sixty medicinal and food plants were screened for antioxidant activity. Antioxidant activity was measured by the TBA (Thiobarbituric acid), DPPH (1, 1-Diphenyl-2-picrylhydrazyl), SOD (superoxide dismutase) which was evaluated by the nitro blue tetrazolium(NBT) reduction method. Among sixty plants, black Glycine max(87. 3%) and Solanum nigrum (80.6%) exhibited the highest antioxidant activity by TBA and DPPH methods, respectively. Also, 10 species extracts including black Glycine max showed the high activity value in these two methods. The SOD characteristics on black Glycine max seed extracts which showed the highest SOD activity (53.5%) exhibited four major SODs; two Cu/ZnSODs and two FeSODs. However, Adenophaora vertidllata which showed lowest SOD value (10.4%) had only Cu/Zn SOD. No varietal differences in the high SOD value were detected in the Cu/Zn SOD isozyme patterns.

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Protective Effects of Sweet Orange, Unshiu Mikan, and Mini Tomato Juice Powders on t-BHP-Induced Oxidative Stress in HepG2 Cells

  • Jannat, Susoma;Ali, Md Yousof;Kim, Hyeung-Rak;Jung, Hyun Ah;Choi, Jae Sue
    • Preventive Nutrition and Food Science
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    • v.21 no.3
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    • pp.208-220
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    • 2016
  • The aim of this study was to investigate the protective effects of juice powders from sweet orange [Citrus sinensis (L.) Osbeck], unshiu mikan (Citrus unshiu Marcow), and mini tomato (Solanum lycopersicum L.), and their major flavonoids, hesperidin, narirutin, and rutin in tert-butyl hydroperoxide (t-BHP)-induced oxidative stress in HepG2 cells. The increased reactive oxygen species and decreased glutathione levels observed in t-BHP-treated HepG2 cells were ameliorated by pretreatment with juice powders, indicating that the hepatoprotective effects of juice powders and their major flavonoids are mediated by induction of cellular defense against oxidative stress. Moreover, pretreatment with juice powders up-regulated phase-II genes such as heme oxygenase-1 (HO-1), thereby preventing cellular damage and the resultant increase in HO-1 expression. The high-performance liquid chromatography profiles of the juice powders confirmed that hesperidin, narirutin, and rutin were the key flavonoids present. Our results suggest that these fruit juice powders and their major flavonoids provide a significant cytoprotective effect against oxidative stress, which is most likely due to the flavonoid-related bioactive compounds present, leading to the normal redox status of cells. Therefore, these fruit juice powders could be advantageous as bioactive sources for the prevention of oxidative injury in hepatoma cells.

Characteristics of Naturalized Plants in the Gwangyang Steel Works (광양제철소 내의 귀화식물상의 특성)

  • Oh, Hyun-Kyung;Kim, Dal-Ho;Kim, Do-Gyun;Nam, Woong
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.12 no.3
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    • pp.9-20
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    • 2009
  • The naturalized plants in the Gwangyang Steel Works were listed 70 taxa; 18 families, 51 genus, 66 species, 3 varieties and 1 form. by field survey. The naturalized plants divided into pattern by survey of annual plants ratio is 31 taxa (44.3%) by life form spectrum and perennials is 23 taxa (32.9%), biennials is 13 taxa (18.5%), two trees (Robinia pseudoacacia, Alianthus altissima) and one shrub (Amorpha furticosa) were founded. According to analysis results form place of origin, Europe covered 26 taxa (37.1%), North America covered 23 taxa (32.8%). Naturalized degree 3 plants, as common but not abundant, founded as 24 taxa (35.0%) were hold most highly ratio, naturalized degree 1 plants founded as 3 taxa (4.0%) were hold lowest. Introduction period 1 covered 31 taxa (44.3%) have had highest score and period 2 covered 11 taxa (15.7%) have had lowest scores. In addition, the urbanization index based on 271 taxa was 25.8% and 2 taxa (Solanum carolinense, Ambrosia artemisiaefolia) is growing in the Gwangyang Steel Works by ecosystem disturbing wild plants. Meanwhile, Ambrosia artemisiaefolia have confirmed into several tens~hundred in the Gwangyang Steel Works were damage the human body of plants develop an allergy to pollen. Solanium carolinense have composed several large communities about $10m{\times}10m$ ($100m^2$) and hold a dominant position, so management plan of the sequel of monitoring them might be required.