• 산업식품공학
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• 제21권2호
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• pp.167-173
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• 2017

본 연구에서는 고유의 자색 안토시아닌을 함유하고 있는 PFPP를 이용하여 매쉬드 포테이토를 제조하고 이의 물리화학적 특성을 확인하였다. PFPP와 전지분유, 덱스트린을 혼합하여 ready-to-eat (RTE) 매쉬드 포테이토 제품을 제조하기 위하여 세 구성 성분의 최적 혼합비를 도출하고자 혼합물 실험방법을 이용하였다. 다양한 배합비에서 매쉬드 포테이토의 색도, 안토시아닌 함량, 유변학적 특성의 차이를 관찰하였으며 PFPP와 전지분유의 배합비에 따라 매쉬드 포테이토의 구조가 변화하여 다른 유변학적 gel 특성을 나타내었다. PFPP의 함량은 주로 적색도와 안토시아닌 함량에 큰 영향을 미쳤으며, 전지분유의 함량은 전지분유의 친수성 및 수용성 특성에 의하여 유변학적 특성의 변화에 영향을 미쳤다. 매쉬드 포테이토의 유변학적 특성 및 다양한 배합비에 따른 특성은 모델링을 통하여 구조적 및 성분에 따른 경향을 정량적으로 나타낼 수 있었다. 본 연구를 통하여 적색도와 안토시아닌의 함량을 최대로 함유하면서 유변학적 특성은 시판되는 매쉬드 포테이토와 유사한 고품질의 기능성 매쉬드 포테이토의 최적 배합비는 PFPP 90.49%, 전지분유 4.86%, 덱스트린 4.65%임을 도출하였다.

• 한국농림기상학회지
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• 제26권2호
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• pp.103-113
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• 2024

남부지역 논에서 봄감자를 재배하고 수확시기를 달리하여 상서 수량과 괴경의 품질 분석 등을 통해 적정 수확시기를 제시하고자 하였다. 봄감자 수량은 개화 후 30-50일에 총수량, 상서수량 등이 최대에 도달하였으며, 상품성 있는 감자의 비율도 높았다. 기형서나 열개서 등 외부생리장해도 개화 후 40일까지는 발생하지 않았으나, 그 이후에는 일부 괴경에서 발아하는 등 2차 생장이 나타났다. 영양성분중에 탄수화물이 60% 이상으로 높고, 개화 후 50일까지는 유의미한 차이를 보이지 않았다. 그리고 조단백질 함량은 수확시기가 늦어짐에 따라 점차 낮아졌다. 괴경의 무기질 함량도 수확시기가 늦어짐에 따라 점차 감소하였으며, 개화 후 30-40일에 가장 낮았다. 따라서 남부지역 봄 감자 재배 시 수확적기는 고온이나 침수의 영향을 덜 받으면서 괴경 비대가 최대에 이르는 개화 후 30-40일인 것으로 판단된다.

• Natural Product Sciences
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• 제6권4호
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• pp.183-188
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• 2000

In order to discover novel potent antitumor agents, methanolic extracts of approximately 180 herbal medicines were prepared and primarily evaluated for cytotoxic activity in cultured human lung (A549) and colon (Col 2) cancer cells. As a result, 17 natural product extracts were found to be active in the criteria of $IC_{50}$<$20\;{\mu}g/ml$. Especially, the extracts of Aristolochia debilis, Cynanchum ascyrifolium, Cynanchum paniculatum, Daphne genkwa, Euphorbia lathyris, Ipomoea hederacea, Magnolia officinalis, Melia azedarach var. japonica, Solanum nigrum, Thuja orientalis, and Trichosanthes kirilowii showed a strong cytotoxic potential. The flower extract of Daphne genkwa was more selective cytotoxic activity against lung cancer cells $(IC_{50};\;0.2\;{\mu}g/ml)$ compared to colon cancer cells $(IC_{50}>20\;{\mu}g/ml)$. In addition, based on the cytotoxic potential of the root extract of Cynanchum paniculatum, the further fractionation of methylene chloride partition with silica gel column chromatography was performed. Several subfractions were considered to be active, and thus indicating that further studies for the identification of active principles from these fractions might be warranted.

• The Plant Pathology Journal
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• 제31권3호
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• pp.252-258
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• 2015

This is the first study reporting the evaluation of transgenic lines of tomato harboring rice chitinase (RCG3) gene for resistance to two important fungal pathogens Fusarium oxysporum f. sp. lycopersici (Fol) causing fusarium wilt and Alternaria solani causing early blight (EB). In this study, three transgenic lines TL1, TL2 and TL3 of tomato Solanum lycopersicum Mill. cv. Riogrande genetically engineered with rice chitinase (RCG 3) gene and their R1 progeny was tested for resistance to Fol by root dip method and A. solani by detached leaf assay. All the R0 transgenic lines were highly resistant to these fungal pathogens compared to nontransgenic control plants. The pattern of segregation of three independent transformant for Fol and A. solani was also studied. Mendelian segregation was observed in transgenic lines 2 and 3 while it was not observed in transgenic line 1. It was concluded that introduction of chitinase gene in susceptible cultivar of tomato not only enhanced the resistance but was stably inherited in transgenic lines 2 and 3.

• 한국환경복원기술학회지
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• 제22권6호
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• pp.41-61
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• 2019

The purpose of this study was carried out to present the basic data for constructing a ecological stream, Shindangcheon stream located in Gyeongju-si, Gyeongsangbuk-do, South Korea. From August 2017 to June 2019, a total of 7 surveys were conducted. The numbers of vascular plants were summarized as 388 taxa including 75 families, 246 genera, 360 species, 4 subspecies, 19 varieties, 2 forms and 3 hybrids. The most families of taxa were Poaceae (59 taxa), Asteraceae (47 taxa), Fabaceae (27 taxa), Rosaceae and Cyperaceae (20 taxa, respectively), and Polygonaceae and Brassicaceae (15 taxa, respectively). The rare plant was Aristolochia contorta Bunge, and the Korean endemic plants were 4 taxa, such as Populus × tomentiglandulosa T.B.Lee, Salix koriyanagi Kimura ex Goerz, Lespedeza maritima Nakai, and Weigela subsessilis (Nakai) L.H.Bailey. The specific plants by floristic region found in this site were 13 taxa comprising 8 taxa of grade I, 4 taxa of grade III, and 1 taxon of grade IV. The naturalized plants were 73 taxa, such as Fallopia dumetorum (L.) Holub, Persicaria orientalis (L.) Spach., Potentilla supina L. var. ternata Pterm., and so forth. The invasive alien plants were 6 taxa, such as Rumex acetosella L., Sicyos angulatus L., Solanum carolinense L., Ambrosia artemisiifolia L., Lactuca serriola L., and Symphyotrichum pilosum (Willd.) G.L.Nesom..

• Interdisciplinary Bio Central
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• 제3권1호
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• pp.1.1-1.6
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• 2011

Recently, with the progress of genome sequencing, materials and information for research on tomato (Solanum lycopersicum) have been systematically organized. Tomato genomics tools including mutant collections, genome sequence information, full-length cDNA and metabolomic datasets have become available to the research community. In Japan, the National BioResource Project Tomato (NBRP Tomato) was launched in 2007, with aims to collect, propagate, maintain and distribute tomato bioresources to promote functional genomics studies in tomato. To this end, the dwarf variety Micro-Tom was chosen as a core genetic background, due to its many advantages as a model organism. In this project, a total of 12,000 mutagenized lines, consisting of 6000 EMS-mutagenized and 6000 gamma-ray irradiated M2 seeds, were produced, and the M3 offspring seeds derived from 2236 EMS-mutagenized M2 lines and 2700 gamma-ray irradiated M2 lines have been produced. Micro-Tom mutagenized lines in the M3 generation and monogenic Micro-Tom mutants are provided from NBRP tomato. Moreover, tomato cultivated varieties and its wild relatives, both of these are widely used for experimental study, are available. In addition to these bioresources, NBRP Tomato also provides 13,227 clones of full-length cDNA which represent individual transcripts non-redundantly. In this paper, we report the current status of NBRP Tomato and its future prospects.

• BMB Reports
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• 제37권3호
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• pp.320-334
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• 2004

StMBF1 (Solanum tuberosum multiprotein bridging factor 1) is a plant member of the MBF1 family of transcriptional co-activators. In an attempt to understand the role of StMBF1, we analyzed its interaction with plant transcription factors of the homeodomain-leucine zipper (Hd-Zip) family, a group of proteins with a typical leucine zipper motif adjacent to a homeodomain. StMBF1 is able to interact in vitro with the Hd-Zip protein Hahb-4 both in the presence and absence of DNA. Upon binding, StMBF1 increases the DNA binding affinity of Hahb-4, and of another plant homeodomain containing protein from the GL2/Hd-Zip IV family, HAHR-1. The biological role of interactions is discussed in this paper.

• The Plant Pathology Journal
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• 제30권4호
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• pp.407-415
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• 2014

Viral diseases have been a major limiting factor threating sustainable potato (Solanum tuberosum L.) production in Pakistan. Surveys were conducted to serologically quantify the incidence of RNA viruses infecting potato; Potato virus X (PVX), Potato virus Y (PVY), Potato virus S (PVS), Potato virus A (PVA), Potato virus M (PVM) and Potato leaf roll virus (PLRV) in two major potato cultivars (Desiree and Cardinal). The results suggest the prevalence of multiple viruses in all surveyed areas with PVY, PVS and PVX dominantly widespread with infection levels of up to 50% in some regions. Co-infections were detected with the highest incidence (15.5%) for PVX and PVS. Additionally the data showed a positive correlation between co-infecting viruses with significant increase in absorbance value (virus titre) for at least one of the virus in an infected plant and suggested a synergistic interaction. To test this hypothesis, glasshouse grown potato plants were challenged with multiple viruses and analyzed for systemic infections and symptomology studies. The results obtained conclude that multiple viral infections dramatically increase disease epidemics as compared to single infection and an effective resistance strategy in targeting multiple RNA viruses is required to save potato crop.

• The Plant Pathology Journal
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• 제33권2호
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• pp.206-211
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• 2017

The effect of temperature on the rate of systemic infection of potatoes (Solanum tuberosum L. cv. Chu-Baek) by Potato virus Y (PVY) was studied in growth chambers. Systemic infection of PVY was observed only within the temperature range of $16^{\circ}C$ to $32^{\circ}C$. Within this temperature range, the time required for a plant to become infected systemically decreased from 14 days at $20^{\circ}C$ to 5.7 days at $28^{\circ}C$. The estimated lower thermal threshold was $15.6^{\circ}C$ and the thermal constant was 65.6 degree days. A systemic infection model was constructed based on experimental data, using the infection rate (Lactin-2 model) and the infection distribution (three-parameter Weibull function) models, which accurately described the completion rate curves to systemic infection and the cumulative distributions obtained in the PVY-potato system, respectively. Therefore, this model was useful to predict the progress of systemic infections by PVY in potato plants, and to construct the epidemic models.

• Journal of Plant Biotechnology
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• 제4권4호
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• pp.155-161
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• 2002

The HCV gene was expressed in potato plants under the control of the constitutive CaMV 355 promoter or tuber-specific patatin promoter. Solanum tuberosum plants carrying a plant expression vector harboring the encoding region of HCV gene were generated by Agrobacterium tumefaciens-mediated in vitro transformation methods. The presence of HCV gene in the plant genome was detected by PCR and DNA hybridization experiments. We obtained the 5 lines of transgenic potato with the pMBPHCV construct and 4 lines of transgenic potato with the pATHCV construct. The HCV transgenic stably integrated into the potato genome, as well as their transcription. HCV mRNA was identified in leaf and tuber tissues of transgenic plants by Northern blot analysis. The transgenic potato plants produced the expected transcript, and the corresponding HCV protein accumulated in individual transgenic plants.