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http://dx.doi.org/10.5423/PPJ.OA.03.2015.0026

Expression of Rice Chitinase Gene in Genetically Engineered Tomato Confers Enhanced Resistance to Fusarium Wilt and Early Blight

Jabeen, Nyla (Department of Bioinformatics and Biotechnology, International Islamic University)
Chaudhary, Zubeda (Department of Botany, Hazara University)
Gulfraz, Muhammad (Department of Chemistry, Comsats Institute of Information Technology)
Rashid, Hamid (Department of Bioinformatics, Mohammad Ali Jinnah University)
Mirza, Bushra (Department of Bioinformatics and Biotechnology, International Islamic University)

Publication Information

The Plant Pathology Journal / v.31, no.3, 2015 , pp. 252-258 More about this Journal

Abstract

This is the first study reporting the evaluation of transgenic lines of tomato harboring rice chitinase (RCG3) gene for resistance to two important fungal pathogens Fusarium oxysporum f. sp. lycopersici (Fol) causing fusarium wilt and Alternaria solani causing early blight (EB). In this study, three transgenic lines TL1, TL2 and TL3 of tomato Solanum lycopersicum Mill. cv. Riogrande genetically engineered with rice chitinase (RCG 3) gene and their R1 progeny was tested for resistance to Fol by root dip method and A. solani by detached leaf assay. All the R0 transgenic lines were highly resistant to these fungal pathogens compared to nontransgenic control plants. The pattern of segregation of three independent transformant for Fol and A. solani was also studied. Mendelian segregation was observed in transgenic lines 2 and 3 while it was not observed in transgenic line 1. It was concluded that introduction of chitinase gene in susceptible cultivar of tomato not only enhanced the resistance but was stably inherited in transgenic lines 2 and 3.

Keywords

early blight; fusarium wilt; rice chitinase gene; tomato;

Citations & Related Records

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