• 한국어병학회지
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• 제19권1호
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• pp.55-64
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• 2006

2003~2004년 봄, 대량 폐사한 가물치 자어 (부화 후 15~30일령)에서 랩도바이러스 유사 병원체를 분리하였다. 분리 바이러스주는 EPC cell과 SSN-1 cell에서 잘 증식하는 반면 FHM cell에는 감수성이 없었고 감염된 EPC cell에서 전형적인 랩도바이러스 모양의 탄환형 바이러스 입자 (45×100 nm)를 다수 관찰할 수 있었다. 분리 바이러스주의 증식 적온은 20~25℃이었고, 15℃이하에서는 증식할 수 없었으며, 30℃ 이상에서는 증식하지는 않았으나 초기 감염가를 유지하였다. 분리 바이러스는 15일령 및 40일령의 가물치 자치어에서는 침지 감염에서 병원성이 높은 반면, 90일령 이상의 중간 육성어에서는 폐사를 나타내지는 않았다.

• 한국어병학회지
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• 제23권1호
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• pp.119-122
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• 2010

Mortality and gill lesions in snakehead, Channa argus (body length range, 20.3-22.2 cm) reared in a aqua-farm in Busan, Korea, were associated with a dense bloom of Apiosoma-like ciliate, Order Petrichida. The size of parasite was $48.83{\pm}7.75{\mu}m{\times}14.29{\pm}2.66{\mu}m$. Histological examination revealed that a severe edema and collapse of the gill tissue were observed in a number of samples of snakehead. The mechanism of gill damage was likely due to physical irritation by the parasite. It is believed that this is the first report of Apiosoma-like ciliate in cultured fish in Korea.

• 한국어병학회지
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• 제33권2호
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• pp.163-169
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• 2020

Snakehead rhabdovirus (SHRV) is different from other fish novirhabdoviruses such as viral hemorrhagic septicemia virus (VHSV), infectious hematopoietic necrosis virus (IHNV), and hirame rhabdovirus (HIRRV) in that it replicates at high temperatures. Therefore, the delivery of foreign proteins to fish living at high water temperature would be possible by using recombinant SHRVs. In the present study, to evaluate the possible use of SHRV as a vehicle for foreign proteins delivery, we generated a recombinant SHRV that contains an enhanced-GFP (eGFP) gene between nucleoprotein (N) and phosphoprotein (P) genes (rSHRV-A-eGFP), and another recombinant SHRV expressing two heterologous genes by inserting an eGFP gene between N and P genes, and mCherry gene between P and M genes (rSHRV-AeGFP-BmCherry). Epithelioma papulosum cyprini (EPC) cells infected with the recombinant SHRVs showed strong fluorescence(s), suggesting the possible availability of recombinant SHRVs for the development of combined vaccines by expressing multiple foreign antigens.

• 수산해양교육연구
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• 제28권3호
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• pp.653-660
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• 2016

Snakehead, Channa argus were intraperitoneally infected with Nocardia seriolae. at the concentrations of $1.5{\times}10^7cfu/m{\ell}$ or $1.5{\times}10^8cfu/m{\ell}$. The infected fish were kept in aquaria at $25^{\circ}C$ and $30^{\circ}C$ for 3 weeks. Clinical signs and mortality were monitored daily to evaluate the virulence. All artificially infected fish showed the same clinical sign found in naturally infected fish. All the fish infected with $1.5{\times}10^8cfu/m{\ell}$ of N. seriolae died within 24days. N. seriolae showed higher virulence to snakehead at the temperature $30^{\circ}C$. Internal lesions such as whitish nodules in the infected internal organs were not correlated with mortality but some degenerative changes were observed in all the infected organs within a week. Whitish nodules in the infected organs which are the typical character in nocardial infection was initially found at two weeks after the artificial infection in snakehead.

• 미생물학회지
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• 제31권2호
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• pp.113-122
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• 1993

본 논문은 양식장에서 자연감염된 궤양성 가물치로부터 우점종으로 분리된 Aeromonas veronii 를 건강한 가물치에 접종한 후, 어체 각 기관의 병변 진행사항을 조직학적 측면에서 연구한 것이다. 양식장내에서 자연 간염된 어체에서의 병변과정은 관찰할 수 없었지만, Aeromonas veronii 로 인공감염시킨 어체의 병변은 자연상태에서 감염된 병변과 매우 유사하였다. 인공감염시킨 어체는 감염후 2일째부터 피부를 비롯하여 아가미, 소화관 조직에 뚜렷한 조직괴사가 식별되었고, 5일 후에는 신장, 사망 직전인 9일 후에는 간과 비장까지 조직의 괴사내지는 세포응축을 나타냈다. 병이 진행됨에 따라 가물치는 피부손상을 비롯하여 호흡, 소화흡수, 배설 등 영양대사의 저해를 수반하면서 감염후 9일이내에 사망하는 것으로 나타났다. 자연감염 어체에서 우점종으로 분리된 균주, Aeromonas veronii 는 가물치의 출혈성 궤양증을 유발하는 주된 균증의 하나로 판단된다.

• Advances in Traditional Medicine
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• 제4권2호
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• pp.91-94
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• 2004

Water and chloroform/methanol extracts of the three local Malaysian snakehead fish, Channa striatus (striped snakehead), Channa micropeltes (giant snakehead) and Channa lucius (blotched snakehead) were evaluated for inhibitory activity in chronic inflammation, using cotton pellet granuloma test. Both water extracts of C. striatus and C. micropeltes showed marked inhibition of the transudative and proliferative components of chronic inflammation (42.9 and 31.2% respectively for C. striatus, 35.6 and 26.2% for C. micropeltes) when compared to those of mefenamic acid (25.1 and 21.3% respectively) and piroxicam (36.1 and 26.2% respectively). The chloroform/methanol extracts did not exhibit any anti-inflammatory effects. These results indicated that C. striatus has more anti-transudative and anti-proliferative activities than the extract of C. micropletes. C. lucius extract in contrast, did not inhibit these two components. This present study indicated the beneficial effects of the water extracts of C. striatus and C. micropeltes, but not C. lucius on chronic inflammation.

• 한국어병학회지
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• 제23권1호
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• pp.113-118
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• 2010

The present work describes myxozoan parasite found in the skin of cultured snakehead, Channa argus fingerings (total length, 5.5~7.2 cm) from Busan, Korea. Nature spores and plasmodia were found in the skin mucus of infected fishes. In fresh state, the total length of the spore was $27.96{\pm}2.50{\mu}m$. The size of spore body was $14.16{\pm}1.78{\mu}m{\times}4.88{\pm}0.61{\mu}m$. The polar capsules were pyriform and the size was $5.57{\pm}0.66{\mu}m{\times}1.36{\pm}0.33{\mu}m$. This is the first report of Henneguya sp. from cultured fish species in Korea, and further studies are necessary for definitive identification.

• 한국어병학회지
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• 제37권1호
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• pp.17-23
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• 2024

Spring viremia of carp virus (SVCV) poses a significant threat to numerous cyprinid fish species, particularly the common carp (Cyprinus carpio), often resulting in substantial mortalities. This study explores the potential use of a chimeric recombinant snakehead rhabdovirus carrying the SVCV G gene (rSHRV-Gsvcv) as a live vaccine against SVCV infection. Through virulence testing in zebrafish at different temperatures (15 ℃ and 20 ℃), no mortality was observed in groups infected with either rSHRV-wild or chimeric rSHRV-Gsvcv at both temperatures, whereas 100% mortality occurred in fish infected with wild-type SVCV. Subsequently, as no mortality was observed by rSHRV-Gsvcv, three independent experiments were conducted to determine the possible usage of chimeric rSHRV-Gsvcv as a vaccine candidate against SVCV infection. Fish were immunized with either rSHRV-Gsvcv or rSHRV-wild, and their survival rates against the SVCV challenge were compared with a control group injected with buffer alone at four weeks post-immunization. The results showed that chimeric rSHRV-Gsvcv induced significantly higher fish survival rates compared to rSHRV-wild and the control groups. These findings suggest that genetically engineered chimeric rSHRV-Gsvcv holds the potential for a prophylactic measure to protect fish against SVCV infection.

• 한국어병학회지
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• 제18권2호
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• pp.157-165
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• 2005

2005년 여름에 국내에서 사육되는 가물치에서 대량폐사를 수반하는 새로운 질병이 발견되었다. 감염 어류는 복부팽만과 항문 주위 출혈 이외의 특이한 외부 증상을 나타내지 않았으나 복부를 절개하면 많은 수의 백색 결절이 간, 비장 및 신장을 포함한 내부 장기에서 관찰되었다. 특히 내부 장기의 조직병리학적 관찰에서 granuloma가 관찰되었다. 이들 감염 어류에서 Gram 양성의 사상균이 분리되었다. 분리균은 Nocardia spp.와 non-Nocardia 균의 16S rRNA 염기 분석에 기초한 Nocardia 특이 primer를 사용한 PCR 방법으로 확인하였다. 이것은 어류에서 발생한 Nocardia 감염증의 국내 첫 사례이다.

• Parasites, Hosts and Diseases
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• 제45권1호
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• pp.45-54
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• 2007

Although Vietnam has a high risk of fish borne zoonotic trematode(FZT) infections for humans, little information exists on the epidemiology of these infections in the country's fish. Because of the importance of cultured cat-fish and snakehead production in An Giang province, a major production area in the Mekong Delta of Vietnam, a survey for FZTs was carried out in randomly selected fish farms between June 2005 and March 2006. For comparison, wild fish from the same area were also surveyed. A total of 852 cultured fish from 4 districts were collected and examined by pepsin digestion to determine their FZT infection status. In Tra catfish, the prevalence of all types of metacercariae was 2.6%, of which the prevalence of Haplorchis pumilio was 0.7%. The overall prevalence of metacercariae in wild fish was 30.6%, of which 10.3% harbored zoonotic species: H. pumilio(2.8%) and Procerovum sp.(5.6%). The prevalence of Opisthorchis metacercariae, which were diagnosed as O. viverrini, was 1.9%. No metacercariae were found in cultured snake head fish, although wild-caught snakehead fish had a FZT prevalence of 10.3%: 5.1% were O. viverrini; 2.6% H. pumilio; and 2.6% were Procerovum sp. These are the first reports of H. pumilio, Procerovum sp., and O. viverrini metacercariae in Vietnamese fish. These results indicate that consumption of improperly prepared fish represents a significant risk of acquiring FZTs in this south Vietnam region.