Browse > Article
http://dx.doi.org/10.13000/JFMSE.2016.28.3.653

Artificial Infection with Nocardia seriolae and the Histological Examination at Snakehead Channa argus  

LEE, Nam-Sil (National Institute of Fisheries Science)
HAN, Hyun-Ja (National Institute of Fisheries Science)
KIM, Myoung-Sug (National Institute of Fisheries Science)
DO, Jeong-Wan (National Institute of Fisheries Science)
JUNG, Sung-Hee (National Institute of Fisheries Science)
CHO, Hyae-In (Gyeongbuk Native Fish Business Center)
KIM, Jin-Do (National Institute of Fisheries Science)
Publication Information
Journal of Fisheries and Marine Sciences Education / v.28, no.3, 2016 , pp. 653-660 More about this Journal
Abstract
Snakehead, Channa argus were intraperitoneally infected with Nocardia seriolae. at the concentrations of $1.5{\times}10^7cfu/m{\ell}$ or $1.5{\times}10^8cfu/m{\ell}$. The infected fish were kept in aquaria at $25^{\circ}C$ and $30^{\circ}C$ for 3 weeks. Clinical signs and mortality were monitored daily to evaluate the virulence. All artificially infected fish showed the same clinical sign found in naturally infected fish. All the fish infected with $1.5{\times}10^8cfu/m{\ell}$ of N. seriolae died within 24days. N. seriolae showed higher virulence to snakehead at the temperature $30^{\circ}C$. Internal lesions such as whitish nodules in the infected internal organs were not correlated with mortality but some degenerative changes were observed in all the infected organs within a week. Whitish nodules in the infected organs which are the typical character in nocardial infection was initially found at two weeks after the artificial infection in snakehead.
Keywords
Artificial infection; Nocardia seriolae; Snakehead;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Chen SC. (1992). The study on the pathogenicity of Nocardia asterroides to largemouth bass Micropterus salmoides Lacepede. Gyobyo Kenkyu 27(1), 1-5.   DOI
2 Chen S.C..Lee J. L..Lai C. C..Gu Y. W..Wang C. T..Chang H. Y. & Tsai K. H.(2000). Nocardiosis in sea bass, Lateolabrax japonicus, in Taiwan. Journal of fish diseases 23, 299-307.   DOI
3 Cornwell E. R..Cinelli M. J..Mcintosh D. M..Blank G. S..Wooster G. A..Groocock G. H..Getchell R. G. & Bowser P. R.(2011). Epizootic Nocardia infection in cultured weakfish, Crynoscion regalis (Bloch and Schneider). Journal of fish diseases 34, 567-571.   DOI
4 Engelsma M. Y..Roozenburg I. & Joly J. P.(2008). First isolation of Nocardia crassostreae from Pacific oyster Crassostrea gigas in Europe. Diseases of aquatic organisms 80, 229-234.   DOI
5 Isik K..Chun J..Hah Y. C. & Goodfellow M.(1999). Nocardia salmonicida nom. rev., a fish pathogen. International Journal of Systematic Bactriology 49, 833-837.   DOI
6 Itano T..Kawakami H..Kono T. & Sakai M.(2006). Experimental induction of nocardiosis in yellowtail, Seriola quinqueradiata Temminck & Schlegel by artificial challenge. Journal of fish diseases 29, 529-534.   DOI
7 Itano T..Kawakami H..Kono T. & Sakai M.(2008). Estimation of the time for Nocardia seriolae infection of cultured yellowtail. Fish Pathology 43(2), 86-88.   DOI
8 Kusuda R..Nakagawa A.(1978). Nocardial infection of cultural yellowtail. Fish pathology 13(1), 25-31.   DOI
9 Labrie L..Ng J..Tan Z..Komar C..Ho E. & Grisez L.(2008). Nocardial infection in fish: An emerging problem on both freshwater and marine aquaculture systems in asia. Diseases in asian aquaculture. Manila, Philippines. 297-312.
10 Lee D. C..Cho M. Y..Park S. I.(2007). A review of nocardial infection in fishes. J. Fish Pathol., 20(1), 1-23.
11 Miyoshi Y..Suzuki S.(2003). A PCR method to detect Nocardia seriolae in fish samples. Fish Pathology 38(3), 93-97.   DOI
12 Snieszko S. F..Bullock C. E..Dunbar C. E. & Pettijon L. L.(1964). Nocardial infection in hatchery-reared fingerling rainbow trout (Salmo gairdneri). Journal of bacteriology 88, 1809-1810.
13 Park M. A..Lee D. C..Cho M. Y..Choi H. j. & Kim J. W.(2005). Mass mortality caused by nocardial infection in cultured snakehead, Channa arga in Korea. J. Fish Pathol., 18(2), 157-165.
14 Park M. A..Cho M. Y..Kim M. S..Kim J. H. & Lee D. C.(2009). Identification of Nocardia seriolae by polymerase chain reaction. 2009. J. Fish Pathol., 22(1), 83-90.
15 Shepperd, M.(2006). Nocardia seriolae A chronic problem. Aquaculture Health, Issue 4, Schering-Plough Animal Health, 25-26.
16 Valdez I. E..Conroy D. A.(1963). The study of a tuberculosis-like condition in neon tetras (Hyphessobrycon innesi). II. Characteristics of the bacterium isolated. Microbiologia Espanola 16, 249-253.
17 Wang G. L..Yuan S. P. & Jin S.(2005). Nocardiosis in large yellow croaker, Larimichthys crocea (Richardson). Journal of fish diseases 28, 339-345.   DOI
18 Wang G. L..Xu Y. J..Jin S..Zhu J. L. & Yuan S. P.(2007). Nocardiosis in snakehead, Ophiocephalus argus Cantor. Aquaculture 271, 54-60.   DOI
19 Wang P. C..Chen S. D..Tsai M. A..Weng Y. J..Chu S. Y..Chern R. S. & Chen S. C.(2009). Nocardia seriolae infection in the Three striped tigerfish, Terapon jarbua (Forsskai). Journal of fish diseases 32, 301-310.   DOI
20 Wolke R. E..Meade T. L.(1974). Nocardiosis in chinook salmon. Journal of wildlife diseases 10, 149-154.   DOI