• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2002년도 심포지엄
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• pp.29-30
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• 2002

The goal of this research was to develop the effectiveness of in vitro culture method for A. formosanus and study the environment in vitro conditions affecting on growth. The first series of experiments were examined to investigate the response of three different basal media, MS (Murashige and Skoog, 1962), Knudson (KC; Knudson, 1946) and modified hyponex on growth and multiplication during in vitro culture. Multiple shoot proliferation was induced in shoot tip explants on Hyponex (H3) media supplemented with BA (1 mg1$\^$-1/) or TDZ (1-2 mg1$\^$-1/). Addition of activated charcoal (1%) to the TDZ containing medium promoted rapid shoot tip proliferation (11.1 shoots per explant) but the same medium had an opposite effect resulting in poor proliferation in the nodal explants. However, the regenerated shoots had slow growth rate and failed to elongate. This problem was overcome by transferring the shoot clumps to a hormone free H3 media supplemented with 2% sucrose and 0.5% activated charcoal. Using bioreactor culture for scaling up was also shown the best way for multiple shoot induction and growth of this plant. The second series of experiments was studied to investigate the effect of physical environment factors on growth of in vitro plantlets. The Anoectochilus formosanus plantlets were cultured under different air exchange rate (0.1, 0.9, 1.2h$\^$-1/), without sucrose or supplement 20g.1$\^$-1/ (photoautotrophic or photomixotrophic, respectively), and different photosynthesis photon flux (40, 80, 120 ,${\mu}$mol.m$^2$.s$\^$-1/- PPF). Under non-enrichment CO$_2$ treatment, slow growth was observed in photoautotrophical condition as compared with photomixotrophical condition on shoot height, fresh weigh and dry weight parameters; High air exchange (1.2.h-l) was found to be inadequate for plant growth in photomixotrophical condition. On the contrary, under CO$_2$, enrichment treatment, the plant growth parameters were sharply (visibly) improved on photoautotrophic treatments, especially on the treatment with air exchange rate of 0.9.h-1. The growth of plant in photoautotrophic condition was not inferior compared with photomixotrophic, and the best growth of plantlet was observed in treatment with low air exchange rate (0.9.h-1). Raising the PPF level from 80 to 120${\mu}$mol.m$\^$-2/.s$\^$-1/ decreased the plant height, particularly at 120${\mu}$mol.m$\^$-2/.s$\^$-1/ in photoautotrophic condition, fresh weight and dry weight declined noticeably. At the PPF of 120${\mu}$mol.m$\^$-2/,s$\^$-1/, chlorophyll contents lowed compared to those grown under low PPF but time courses of net photosynthesis rate was decreased noticeably. Light quality mainly affected morphological variables, changes of light quality also positively affected biomass production via changes in leaf area, stem elongation, chlorophyll content. Plant biomass was reduced when A. formosanus were grown under red LEDs in the absence of blue wavelengths compare to plants grown under supplemental blue light or under fluorescent light. Stem elongation was observed under red and blue light in the present experiment. Smaller leaf area has found under blue light than with other lighting treatments. Chlorophyll degradation was more pronounced in red and blue light compared with white light or red plus blue light which consequent affected the photosynthetic capacity of the plant. The third series of experiment were studied to investigate the effect of physical environment factors on growth of ex vitro plants including photosynthesis photon flux (PPF), light quality, growing substrates, electrical conductivity (EC) and humidity conditions. In the present experiments, response of plant on PPF and light quality was similar in vitro plants under photosynthesis photon flux 40${\mu}$mol.m,$\^$-2/.s$\^$-1/ and white light or blue plus red lights were the best growth. Substrates testing results were indicated cocopeat or peat moss were good substrates for A. formosanus growth under the greenhouse conditions. In case of A. formosanus plants, EC is generally maintained in the range 0.7 to 1.5 dS.m-1 was shown best results in growth of this plant. Keeping high humidity over 70% under low radiation enhanced growth rate and mass production.

• PNF and Movement
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• 제14권2호
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• pp.121-130
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• 2016

Purpose: The purpose of this study was to investigate the effects of visual and auditory stimulation on the ability to perform exercise. Methods: One hundred twenty subjects were randomly divided into four groups (Green light and Fast tempo music, GF; Green light and Slow tempo music, GS; Red light and Fast tempo music, RF; and Red light and Slow tempo music, RS). One of either two visual stimuli or one of two auditory stimuli were applied to each group. The experiment was conducted randomly twice in two environments: one had visual and auditory stimuli and one had no stimulation. Muscle strength, grip, endurance, quickness, agility, concentration, and balance were measured to determine the ability to perform exercise. Results: Significant differences were found in the muscle strength of the participants who were exposed to the auditory factor and the interaction of visual and auditory factors. In endurance, significant differences were found in all of the factors: visual, auditory, and the interaction of visual and auditory. In quickness, agility, and balance ability there were significant differences in the visual factor. In concentration, there was a significant difference in the auditory factor. Conclusion: Visual stimuli, auditory stimuli, and their interaction influenced the ability to perform exercise. These facts imply that providing the proper environmental stimulation is important to increase the ability to perform during exercise.

• 천문학논총
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• 제32권3호
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• pp.421-438
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• 2017

Through the short-period variability survey program, we obtained time-series BV CCD images for $1.5^{\circ}{\times}1.0^{\circ}$ region around the young open cluster NGC 457. As a result, we have detected 61 variable stars including 31 new ones after checking light curves of all stars by eyes. The 61 variable stars were included 14 ${\delta}$ Scuti variable stars, a ${\beta}$ Cephei variable star, 10 variable Be and slowly pulsating B stars, 13 eclipsing binary stars, 21 semi-long periodic or slow irregular variables and an RR Lyrae variable star, respectively. Many variable B-type stars were known through a well-defined zero-age main sequence to the ${\beta}$ Cepheid region of NGC 457. Most of the variable B-type stars found this paper were known variable stars. But, 11 out of 14 ${\delta}$ Scuti variable stars were newly discovered. The new variable stars except for ${\delta}$ Scuti stars were 4 variable B-type stars, 5 eclipsing binaries and 11 semi-long periodic or slow irregular variables. We have performed frequency analysis for all ${\delta}$ Scuti stars, a ${\beta}$ Cepheid star and an RR Lyrae star.

• 한국표면공학회지
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• 제41권3호
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• pp.109-113
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• 2008

High strength 7xxx series Al-Zn-Mg alloy have been investigated for using light weight automotive parts especially for bump back beam. The composition of commercial 7xxx aluminum has the Zn/Mg ratio about 3 and Cu over 2 wt%, but this composition isn't adequate for appling to automotive bump back beam due to its high resistance to extrusion and bad weldability. In this study the Zn/Mg ratio was increased for better extrusion and Cu content was reduced for better welding. With this new composition we investigated the effect of composition on the resistivity against stress corrosion cracking. As the Zn/Mg ratio is increased fracture energy obtained by slow strain rate test was decreased, which means degradation of SCC resistance. While the fracture energy was increased with Cu contents although it is below 1%, which means improvement of SCC resistance. These effects of composition change on the SCC resistivity were identified by observing the fracture surface and crack propagation.

• 대한지리학회지
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• 제45권3호
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• pp.375-389
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• 2010

한라산 표고 1,710m에 발달한 나지 표층에 2개의 페인트 라인을 설치하여 자갈의 이동량을 관측하고, 이동 프로세스와 관련된 요인을 검토하였다. 관측 기간에 발생한 자갈의 이동량은 평균 58.2cm로서 일평균 이동 거리는 0.24cm이다. 계절별로 비교하면 동결기 0.3cm에 대하여 비동결기는 0.05cm에 불과하여 표층 자갈은 주로 동결기에 주빙하 프로세스를 통하여 이동하고 있다. 특히 서릿발 포행을 비롯한 동상 포행이 자갈의 이동을 주도하고 있다. 사면 경사각의 영향은 관측 기간 내내 측선 II의 이동량이 측선 I보다 큰 결과에 반영되어 있지만, 암괴와 식생이 자갈의 이동을 방해하므로 절대적이지는 않다. 자갈의 크기와 비중도 이동량에 관여하는 요인으로서 특히 스코리아의 작은 비중이 매우 큰 이동량을 가져왔다.

• 대한교통학회지
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• 제21권1호
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• pp.91-102
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• 2003

본 연구는 $\circled1$Cellular Automata(이하 CA)모형을 기반으로 대규모 네트워크에 적용 가능한 보다 현실적인 CA차량모형 구축. $\circled2$구축된 CA차량모형을 이용한 차량 모의실험기의 개발과 개발된 차량 모의실험기를 이용한 단기링크통행시간 예측으로 구성된다. 구축된 CA차량추종모형은 기존의 CA차량추종모형 보다 현실적으로 감속을 통한 정지과정을 설명하면서 거시적 지표인 교통량-밀도-속도관계를 설명하였다. 또한 링크의 유출교통량(Outflow)을 제어하기 위한 차량의 링크전이모형은 기존의 차량 링크전이모형에 비하여 보다 안정된 대기차량을 형성하였다. 단기링크통행시간 예측을 위한 차량모의실험기는 대규모 가로망에 적용이 가능하도록 차량묶음(Packet, 이하차량묶음)방식과 링크기반 모의실험방식으로 컴퓨터의 연산 수행속도 및 메모리를 효율적으로 처리할 수 있었으며, 기존의 시계열자료 예측기법에서 고려할 수 없었던 차량의 행태 및 링크 상에서 발생하는 이동류 과포화, 뒷막힘현상 등의 메커니즘을 고려함으로서 기존 시계열자료 예측기법에 비하여 우수한 예측력을 보였다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2013년도 제44회 동계 정기학술대회 초록집
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• pp.669-669
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• 2013

Hierarchical N doped TiO2 nanostructured catalyst with micro, meso and macro porosity have been synthesized by a facile self-formation route using ammonia and titanium isopropoxide precursor. The samples were calcined in different calcination temperature ranging from $300^{\circ}C$ to $800^{\circ}C$ at slow heating rate ($5^{\circ}C$/min) and designated as NHPT-300 to NHPT-800. $TiO_2$ nanostructured catalyst have been characterized by physico-chemical and spectroscopy methods to explore the structural, electronic and optical properties. UV-Vis diffuse reflectance spectra confirmed the red shift and band gap narrowing due to the doping of N species in TiO2 nanoporous catalyst. Hierarchical macro porosity with fibrous channel patterning was observed (confirmed from FESEM) and well preserved even after calcination at $800^{\circ}C$, indicating the thermal stability. BET results showed that micro and mesoporosity was lost after $500^{\circ}C$ calcination. The photocatalytic activity has been evaluated for methanol oxidation to formaldehyde in visible light. The enhanced photocatalytic activity is attributed to combined synergetic effect of N doping for visible light absorption, micro and mesoporosity for increase of effective surface area and light harvestation, and hierarchical macroporous fibrous structure for multiple reflection and effective charge transfer.

• The Korean Journal of Physiology and Pharmacology
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• 제28권1호
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• pp.49-57
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• 2024

While arterial tone is generally determined by the phosphorylation of Ser¹⁹ in myosin light chain (p-MLC2), Thr¹⁸/Ser¹⁹ diphosphorylation of MLC2 (pp-MLC2) has been suggested to hinder the relaxation of smooth muscle. In a dual-wire myography of rodent pulmonary artery (PA) and mesenteric artery (MA), we noticed significantly slower relaxation in PA than in MA after 80 mM KCl-induced condition (80K-contraction). Thus, we investigated the MLC2 phosphorylation and the expression levels of its regulatory enzymes; soluble guanylate cyclase (sGC), Rho-A dependent kinase (ROCK) and myosin light chain phosphatase target regulatory subunit (MYPT1). Immunoblotting showed higher sGC-α and ROCK2 in PA than MA, while sGC-β and MYPT1 levels were higher in MA than in PA. Interestingly, the level of pp-MLC2 was higher in PA than in MA without stimulation. In the 80K-contraction state, the levels of p-MLC2 and pp-MLC2 were commonly increased. Treatment with the ROCK inhibitor (Y27632, 10 µM) reversed the higher pp-MLC2 in PA. In the myography study, pharmacological inhibition of sGC (ODQ, 10 µM) slowed relaxation during washout, which was more pronounced in PA than in MA. The simultaneous treatment of Y27632 and ODQ reversed the impaired relaxation in PA and MA. Although treatment of PA with Y27632 alone could increase the rate of relaxation, it was still slower than that of MA without Y27632 treatment. Taken together, we suggest that the higher ROCK and lower MYPT in PA would have induced the higher level of MLC2 phosphorylation, which is responsible for the characteristic slow relaxation in PA.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2010년도 춘계학술대회 초록집
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• pp.87-87
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• 2010

Photoelectrochemical (PEC) systems are promising methods of producing H2 gas using solar energy in an aqueous solution. The photoelectrochemical properties of numerous metal oxides have been studied. Among them, the PEC systems based on TiO2 have been extensively studied. However, the drawback of a PEC system with TiO2 is that only ultraviolet (UV) light can be absorbed because of its large band gap (3.2 - 3.4 eV). Two approaches have been introduced in order to use PEC cells in the visible light region. The first method includes doping impurities, such as nitrogen, into TiO2, and this technique has been extensively studied in an attempt to narrow the band gap. In comparison, research on the second method, which includes visible light water splitting in molecular photosystems, has been slow. Mallouk et al. recently developed electrochemical water-splitting cells using the Ru(II) complex as the visible light photosensitizer. the dye-sensitized PEC cell consisted of a dye-sensitized TiO2 layer, a Pt counter electrode, and an aqueous solution between them. Under a visible light (< 3 eV) illumination, only the dye molecule absorbed the light and became excited because TiO2 had the wide band gap. The light absorption of the dye was followed by the transfer of an electron from the excited state (S*) of the dye to the conduction band (CB) of TiO2 and its subsequent transfer to the transparent conducting oxide (TCO). The electrons moved through the wire to the Pt, where the water reduction (or H2 evolution) occurred. The oxidized dye molecules caused the water oxidation because their HOMO level was below the H2O/O2 level. Organic dyes have been developed as metal-free alternatives to the Ru(II) complexes because of their tunable optical and electronic properties and low-cost manufacturing. Recently, organic dye molecules containing multi-branched, multi-anchoring groups have received a great deal of interest. In this work, tri-branched tri-anchoring organic dyes (Dye 2) were designed and applied to visible light water-splitting cells based on dye-sensitized TiO2 electrodes. Dye 2 had a molecular structure containing one donor (D) and three acceptor (A) groups, and each ended with an anchoring functionality. In comparison, mono-anchoring dyes (Dye 1) were also synthesized. The PEC response of the Dye 2-sensitized TiO2 film was much better than the Dye 1-sensitized or unsensitized TiO2 films.

• 대한전자공학회논문지SD
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• 제38권8호
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• pp.27-33
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• 2001

파장을 스케닝하기 위해 Fabry-Perot 필터를 사용하는 기존의 광섬유 브래그 격자(FBG) 스트레인 측정시스템은 PZT의 히스테리시스 특성과 필터의 느린 스케닝 시간 그리고 높은 가격으로 인해 응용이 제한적이다. 광원으로 비교적 저가인 발광 다이오드(LED)를 사용하고 FBG 센서를 다중화하기 위해 코드 분할 다중화(CDM) 방식을 도입한 다중 FBG 센서 시스템을 제안하였으며 실험을 통해 특성을 측정하였다. 실험은 정적 스트레인과 동적 스트레인을 측정하기 위한 두 가지 구조의 시스템에 대한 출력과 센서 신호간의 누화를 측정하였다. 구성한 시스템은 매우 저가이고 빠른 응답속도를 갖으며 누화의 레벨은 -30 dB 이하로 측정되었다.