• 대한화장품학회지
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• 제41권3호
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• pp.229-235
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• 2015

피부 노화는 피부 표면에 나타나는 현상으로 피부 표면의 미세구조와 연관이 있다. 피부 미세구조의 변화는 주름형성, 피부 톤 저하 등 다양한 피부 노화 현상을 유발하는 요인이 된다. 본 연구는 주름 기능성 성분인 세드롤(Cedrol)과 콜라겐 유래 펩타이드 성분을 이용하여 시험관 수준(in vitro)에서 타입III 콜라겐 합성 시너지 효과를 평가하였다. 여성 피시험자를 대상으로 4주 동안 상기 원료가 함유된 크림제품을 사용한 후 피부 미세구조, 스타 형상(star configurations), 보습, 탄력, 피부 밝기, 윤기, 피부 톤, 투명도를 평가하였다. 시험관 수준의 세드롤과 펩타이드를 동시에 처리하면 우수한 수준의 타입III 콜라겐 합성 시너지효과를 나타내었다. 그리고 크림제형을 이용한 인체적용 시험에서는 사용 4주 후부터 피부 미세구조, 스타형상, 윤기, 피부 톤, 보습, 탄력을 개선시켰으나, 피부 밝기 개선 효능은 나타나지 않았다. 본 연구를 통하여 기존 주름 기능성 성분인 세드롤과 콜라겐 유래 펩타이드는 세포 수준에서 타입III 콜라겐 합성 시너지 효과가 있음을 확인하였고 이를 화장품에 적용하여 시험한 결과 피부 노화 개선에 도움을 주는 성분임을 확인하였다.

• 대한화장품학회지
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• 제39권4호
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• pp.323-327
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• 2013

수분량, 유분량, 피부밝기, pH 등 피부 특성을 나타내는 지표들은 환경적, 유전적 요소에 따라 다르게 나타난다. 하지만 이는 절대적인 피부 특성을 나타내는 것으로 사람들이 느끼는 감성적인 피부 특성과는 차이가 있다. 이를 반영하듯 최근 임상 연구들은 절대적인 피부 변화를 통한 사람들의 인지 변화에 관한 연구들이 주를 이루고 있다. 본 연구에서는 아시아인을 대상으로 국가별로 절대적인 피부색의 차이 뿐 아니라 실제 피부색의 변화에 따라 본인들이 인지하는 피부 밝기에도 차이가 있는지를 규명하고자 하였다. 아시아 3개국 총 410명의 피험자들이 본 연구에 참여하였으며 설문을 통해 본인들이 생각하는 피부밝기를 3단계로 구분하여 응답하고 본인이 생각하는 피부 밝기 변화에 따라 실제 피부색은 어떤 양상으로 변화하는지를 분석하였다. 국적에 관계 없이 모든 참가자들이 공통적으로 피부색이 밝다고 느낄수록 실제 피부 밝기는 증가하는 양상을 보였지만 절대적인 피부색과 피부가 밝다고 느끼는 정도는 차이가 있었다. 게다가, 피부 붉은기와 노란기의 변화도 국가별로 다른 양상을 보였다. 결론적으로, 본 연구 결과에 근거하여 판단 할 때 본인들의 피부 밝기를 인지하는 요소는 절대적인 피부 밝기가 아닌 국가별로 다른 기준이 적용된다는 것을 확인할 수 있었다.

• Preventive Nutrition and Food Science
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• 제17권4호
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• pp.245-253
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• 2012

Collagen tripeptide (CTP) is a functional food material with several biological effects such as improving dry skin and wound and bone fracture healing. This study focused on the anti-photoaging effects of CTP on a hairless mouse model. To evaluate the effects of CTP on UVB-induced skin wrinkle formation in vivo, the hairless mice were exposed to UVB radiation with oral administration of CTP for 14 weeks. Compared with the untreated UVB control group, mice treated with CTP showed significantly reduced wrinkle formation, skin thickening, and transepidermal water loss (TEWL). Skin hydration and hydroxyproline were increased in the CTP-treated group. Moreover, oral administration of CTP prevented UVB-induced MMP-3 and -13 activities as well as MMP-2 and -9 expressions. Oral administration of CTP increased skin elasticity and decreased abnormal elastic fiber formation. Erythema was also decreased in the CTP-treated group. Taken together, these results strongly suggest that CTP has potential as an anti-photoaging agent.

• 한방안이비인후피부과학회지
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• 제24권3호
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• pp.84-107
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• 2011

Purposes : The purpose of present study is to detect the effects of ointment contained Takrisodok-Eum on the skin barrier's recovery of the Atopic dermatitis. Methods : A total of 44 patients who visited Semyung Oriental Medical Center from April 27th, 2011 to September 20th, 2011 were included in the study. In this study, the patients were treated with Takrisodok-Eum (experimental group) and positive control group. For 8 weeks of gross examination, hematological examination and instrumental asessment were made before and after the study to examine how well the products for experimental group with those for control group in recovering the damaged skin barriers by Atopic dermatitis. Results : 1. In the primary endpoint, SCORAD Index showed a statistically significant decline in experimental group. 2. In the secondary endpoint index of skin hydration, both the experimental group and the control group showed statistical significance. 3. In the secondary endpoint index of TEWL, the experimental group showed greater statistical significance than the control group. 4. In the secondary endpoint index of skin pH, skin pH showed a statistically significant decline in both the control group and the experimental group within the normal range. 5. In the secondary endpoint index of Total IgE, Eosinophil count, IL-10, IL-13, both the experimental group and the control group showed no statistical significance. 6. To evaluate the safety of the products for the human body, hematological examination and hematological biochemical examination, vital sign check were conducted; both control group and experimental group showed no abnormal level. And There were no severe adverse events during this study. Therefore, it is suggested that the safety of the products, if used for certain period, should be safe for the human body. Conclusions : According to the above experiments, it is suggested that Takrisodok-Eum should be effective for the Atopic dermatitis.

• 한방안이비인후피부과학회지
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• 제22권1호
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• pp.120-132
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• 2009

Objective : Yukmijihwang-tang is one of the important medicines for blood- deficiency and yin-deficiency. Atopic dermatitis usually shows dampness-heat pattern in its acute stage and blood-deficiency or yin-deficiency pattern in its chronic stage. Therefore, I hypothesized that Yukmijihwang-tang is effective on atopic dermatitis and investigated the effects of Yukmijihwang-tang on NC/Nga mice's atopic dermatitis induced by DNCB. Methods : The NC/Nga mice with atopic dermatitis were divided into four groups: three experimental groups and one control group. The experimental groups were respectively put on 2.5g(YM-2.5), 5g(YM-5) and 10g(YM-10) of Yukmijihwang-tang extract per their weight once a day for 10 days while the control group was fed normal saline. After 10 days, I measured TEWL(transepidermal water loss), observed scratching behaviors, conducted a skin biopsy and checked levels of Total IgE, IL-4 and $IFN-\gamma$ on NC/Nga mice. Results : 1. Yukmijihwang-tang significantly suppressed skin dryness. In particular, YM-5 and YM-10 had better skin hydration than YM-2.5. 2. Yukmijihwang-tang significantly suppressed pruritus while there was no significant difference among the experimental groups. 3. Yukmijihwang-tang retained skin structure(epidermis, dermis and subcutaneous fat). 4. Yukmijihwang-tang reduced Total IgE level while there was no significant difference between the control group and the experimental groups. 5. Yukmijihwang-tang did not reduce IL-4(Th2 cytokine) level. 6. Yukmijihwang-tang significantly reduced $IFN-\gamma$(Th1 cytokine) level. In particular, YM-2.5 and YM-5 had lower $IFN-\gamma$ level than YM-10. Conclusion : The results suggest that Yukmijihwang-tang suppresses skin dryness and pruritus, retains skin structure and is effective on chronic atopic dermatitis which is associated with Th1 cytokines in the immune response.

• 대한화장품학회지
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• 제40권3호
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• pp.247-257
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• 2014

새로운 베지클인 glyceryl citrate/ lactate/ linoleate/ oleate를 이용한 수중유형 형태의 아스타잔틴 나노에멀젼에 대해 항산화 효과, 세포 생존력, 단백질과 관련한 효소의 영향, 피부 침투도 그리고 피부에 대한 보습 및 탄력 등의 약용화장품적인 측면에서의 전반적 연구를 실시하였다. 항산화력 및 세포 생존력에 대해선 각각 DPPH법과 MMT assay를 이용하여 측정하였다. 아스타잔틴 나노에멀젼에 대한 또 다른 성질은 2D-Page를 이용한 단백질 분석 및 컨포칼, in-vivo 테스트를 통해 측정하였다. 본 연구를 통해, 아스타잔틴을 포함하는 나노에멀젼은 MMP발현에 관련한 단백질 억제 및 세포외 기질의 분해를 막고 라디칼의 소거에 매우 우수한 결과를 보였다. 종전의 레시친을 이용한 나노에멀젼 보다는 새로운 베지클을 이용한 아스타잔틴 나노에멀젼의 피부 침투가 매우 효과적임을 CLSM을 통해 측정하였다. 또한 28일 동안의 한국 성인 여성 11명을 통한 보습 및 탄력 인비보 테스트에서 우수한 효과를 확인할 수 있었다.

• 한방안이비인후피부과학회지
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• 제36권4호
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• pp.113-121
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• 2023

Objectives : The purpose of this study is to analyze the treatment mechanism of Thread-embedding acupuncture, which is used in Korean medicine beauty treatment. Methods : After searching papers published up to January 1, 2023 using the keyword "Thread-embedding" through the OASIS site, we selected beauty papers that mentioned the treatment mechanism of Thread-embedding acupuncture. Results : A total of 60 papers were retrieved: 19 papers on the topic of cosmetic diseases, 35 papers on the theme of other diseases, and 6 papers written unrelated to diseases. Among the 19 papers on the topic of cosmetic diseases, one unreadable paper was excluded. Among the 18 papers, we finally selected 6 papers that mentioned treatment mechanisms: 2 on facial wrinkles, 2 on obesity, 1 on breast enlargement, and 1 on transdermal hydration. The treatment mechanism of Thread-embedding acupuncture is that in the case of facial wrinkles, polydioxanone(PDO) is embedded to fill the volume, and as it decomposes, it causes a tissue reaction around the area. In obesity, it promotes fat decomposition by improving circulation, and promotes breast enlargement and elasticity through collagen formation. In transdermal hydration, it induces the production of surrounding fibers to increase skin elasticity and moisture. Conclusions : Thread-embedding acupuncture appears to have a cosmetic effect through a mechanism that promotes the production of collagen and elastic fibers in the subepidermal dermal layer and increases the activity of skin moisturizing factors during the absorption process after the PDO suture is embedded.

• 대한화장품학회지
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• 제22권1호
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• pp.27-39
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• 1996

최근 in vitro 각질층 모델은 TEWL, 수분보유능 등 피부의 상태변화를 측정하기 위한 유용한 수단으로 이용되고 있다. 본 연구에서는 노화에 따른 각질층의 상태 변화를 조사하기 위하여 in vitro 각질층 모델을 이용한 TEWL 측정, 각질층의 지질분석, 최외각 각질세포의 크기를 측정하였다. 실험 결과, 8주령 무모생쥐의 전체지질은 82 주령 무모생쥐보다 30%가량 많았으나 TEWL은 8주령 무모생쥐가 82주령된 무모생쥐보다 더 높았다. 또한 최외각 각질세포의 크기는 82주령의 무모생쥐가 8주령 보다 더 컸다. 따라서 각질층의 장벽기능은 노화에 따라 오히려 강화되고 있으며 이는 노화에 따른 피부의 활성저하에 대응한 일련의 현상으로 간주된다.

• 한방안이비인후피부과학회지
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• 제26권1호
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• pp.75-81
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• 2013

Objectives: Hyaluronic acid, high molecular glycosaminoglycan, exists in extracellular matrix of tissue, especially, in skin and has been known to be deeply involved in skin hydration. In this study, we investigated the effect of methanol extract of Hwang-gi, Astragalus membranaceus root, on hyaluronic acid production in human keratinocyte HaCaT cells. Methods: We determined hyaluronic acid synthase 2 gene expression and hyaluronic acid production in HaCaT cells by using RT-PCR and ELISA, respectively. Results: Hwang-gi extract didn't show the toxicity to HaCaT cells within the treated concentration and increased the hyaluronic acid synthase 2 gene expression and hyaluronic acid production. Conclusions: Hyaluronic acid production increased by Hwang-gi could be, partially, contribute to the moisturing effect in skin by it.

• Journal of Pharmaceutical Investigation
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• 제28권1호
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• pp.43-50
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• 1998

Niosomes are vesicles formed from synthetic non-ionic surfactants, offering an alternative to chemically unstable and expensive liposomes as a drug carrier. Non-ionic surfactant and cholesterol mixture film leads to the formation of vesicular system by hydration with sonication method. The formation of niosome was ascertained by negative staining of TEM. The entrapment efficiency of niosomal suspension was gradually increased with increasing the ratio of cholesterol to surfactant. It was found that the niosome with 6 : 4 (polyoxyethylene 2-cetyl ether: cholesterol) ratio was more stable than those with other ratios. The topical application of acyclovir(ACV) in the treatment of herpes simplex virus type 1(HSV-1) skin disease has a long history. There are an increasing number of reports, however, in which topical ACV therapy is not as effective as oral administration. Lack of efficacy with topical ACV has been hypothesized to reflect the inadequate delivery of drug to the skin. We investigated the permeation of niosome containing $[^{3}H]ACV$ in hairless mouse skin using Franz diffusion cell model. Permeation coefficient(P) of aqueous ACV was $6.7{\times}10^{-4}\;(cm/hr)$ and that of ACV in niosome was $23.4{\times}10^{-4}\;(cm/hr)$, suggesting about 3.5 times increase in the transdermal permeation.