• 대한화장품학회지
• /
• 제45권2호
• /
• pp.175-184
• /
• 2019

상피세포성장인자(epidermal growth factor, EGF)는 인간의 표피 및 진피에서 세포막 수용체와 상호작용을 통하여 세포의 생장 및 증식을 유도하는 기능을 갖고 있다. 이 같은 EGF의 기능은 의료 및 화장품 분야에서 상처치유 의약품 및 노화방지 화장품의 주요원료로 사용되고 있다. 화장품 원료로서 EGF는 피부장벽으로 알려져 있는 피부 각질층의 투과가 잘 안되기 때문에 가지고 있는 본연의 효능을 구현하는 데 문제가 있다. 본 연구에서는 EGF의 경피투과 효율을 개선하기 위하여 피부 투과능이 확인된 거대분자 전송 도메인(macromolecule transduction domain, MTD) 151이 융합된 형태로 재조합 인간 상피세포성장인자 ($MTD_{151}-EGF$)를 개발하였다. $MTD_{151}-EGF$의 유전자가 coding된 vector로 형질전환된 대장균에서 $MTD_{151}-EGF$ 발현시킨 후 정제를 진행하였다. 정제된 MTD-EGF를 대상으로 세포증식시험, 세포독성시험, 생체외 피부흡수시험 그리고 인공피부를 이용한 경피투과능을 평가하였다. 99% 이상 고순도로 정제된 $MTD_{151}-EGF$의 세포증식 활성은 EGF 대비 동등 이상의 수준이었으며, 세포독성은 관찰되지 않았다. 또한, 인공피부 투과모델에서 FITC로 표지된 EGF와 $MTD_{151}-EGF$의 진피층까지의 투과를 공초점 현미경으로 관찰한 결과, $MTD_{151}-EGF$는 EGF 대비 우수한 투과능을 보였으며, 경피흡수 시스템을 이용한 투과물질의 정량분석 결과, EGF 대비 약 16 배 이상 투과량이 많은 것으로 확인되었다. 이러한 결과들은 다양한 활성물질들의 화장품용 원료로서의 경피투과에 MTD가 기존의 물리적인 경피투과 방법을 효율적으로 개선한 대안이 될 것으로 판단된다.

• 한방안이비인후피부과학회지
• /
• 제25권2호
• /
• pp.1-19
• /
• 2012

Objective : The purpose of this study is to investigate about comparison of Eastern-Western medicine on the acne. Methods : We searched Eastern and Western medicine books for acne. We analyzed these books and examined category, definition, etiology, classification, internal and external methods of treatment of acne. Results : The results were as follows. 1. In Eastern medicine, Acne belongs to the category of the Bunja(粉刺), Jwachang(痤瘡), Pyepungbunja(肺風粉刺). In Western medicine, the other name of Acne is acne vulgaris. 2. In Eastern medicine, the definition of Acne includes manual extraction of comedones and skin appearance. In Western medicine, Acne is a common skin disease during adolescence and a chronic inflammatory disease of pilosebaceous unit of self localization. It is characterized by noninflammatory, open or closed comedones and by inflammatory papules, pustules, and nodules and it affects the areas of skin with the densest population of sebaceous follicles, these areas include the face, neck, back, and the upper part of the chest. 3. In Eastern medicine, the cause and mechanism of Acne arose from the state of internal dampness-heat and spleen-stomach internal qi deficiency due to dietary irregularities and then invaded external pathogen such as wind-dampness-heat-cold-fire in lung meridian lead to qi and blood heat depression stagnation. So it appears in skin. In Western medicine, the etiology and pathogenesis of Acne is clearly not identified, but there are most significant pathogenic factors of blood heat depression stagnation. So it appears in skin. In Western medicine, the etiology and pathogenesis of Acne is clearly not identified, but there are most significant pathogenic factors of Acne; Androgen-stimulated production of sebum, hyperkeratinization and obstruction of sebaceous follicles, proliferation of Propionibacterium acnes and inflammation, abnormaility of skin barrier function, genetic aspects, environmental factors etc. 4. In Eastern medicine, differentiation of syndromes classifies clinical aspects, and cause and mechanism of disease; the former is papular, pustular, cystic, nodular, atrophic, comprehensive type; the latter is lung blood heat, intestine-stomach dampness-heat, phlegm-stasis depression, thoroughfare-conception disharmony, heat toxin type. In Western medicine, it divides into an etiology and invasion period, and clinical aspects; Acne neonatorum, Acne infantum, Acne in puberty and adulthood, Acne venenata; Acne vulgaris, Acne conglobata, Acne fulminans, Acne keloidalis. 5. In Eastern medicine, Internal methods of treatment of Acne are divided into five treatments; general treatments, the treatments of single-medicine and experiential description, the treatments depending on the cause and mechanism of disease, and clinical differentiation of syndromes, dietary treatments. In Western medicine, it is a basic principles that regulation on production of sebum, correction on hyperkeratinization of sebaceous follicles, decrease of Propionibacterium acnes colony and control of inflammation reaction. Internal methods of treatment of Acne are antibiotics, retinoids, hormone preparations etc. 6. In Eastern medicine, external methods of treatment of Acne are wet compress method, paste preparation method, powder preparation method, pill preparation method, acupuncture and moxibustion therapy, ear acupuncture therapy, prevention and notice, and so on. In Western medicine, external method of treatments of Acne are divided into topical therapy and other surgical therapies. Topical therapy is used such as antibiotics, sebum regulators, topical vitamin A medicines etc and other surgical therapies are used such as surgical treatments, intralesional injection of corticosteroids, skin dermabrasion, phototherapy, photodynamic therapy, and so on. Conclusions : Until now, there is no perfect, effective single treatment. We think that Eastern medicine approach and treatment can be helpful to overcome the limitations of acne cure.

• Archives of Plastic Surgery
• /
• 제43권6호
• /
• pp.586-589
• /
• 2016

A pressure sore wound is often extensive or complicated by local infection involving adjacent soft tissue and bone. In this case, a regional flap after simple debridement is not adequate. Here, we present a case of an extensive pressure sore in the sacral area with deep tissue infection. A 43-year-old female patient with a complicated sore with deep tissue infection had a presacral abscess, an iliopsoas abscess, and an epidural abscess in the lumbar spine. After a multidisciplinary approach performed in stages, the infection had subsided and removal of the devitalized tissue was possible. The large soft tissue defect with significant depth was reconstructed with a free latissimus dorsi musculocutaneous flap, which was expected to act as a local barrier from vertical infection and provide tensionless skin coverage upon hip flexion. The extensive sacral sore was treated effectively without complication, and the deep tissue infection completely resolved. There was no evidence of donor site morbidity, and wheelchair ambulation was possible by a month after surgery.

• 대한의생명과학회지
• /
• 제18권2호
• /
• pp.175-179
• /
• 2012

It is desirable that a wound healing membrane acts as a barrier for coverage of a damaged skin and has the biological activities such as anti-inflammatory effects. In this study, we prepared the hydrogel film containing the propolis nanoparticles as a wound healing membrane. The propolis nanoparticles were prepared by incorporation of propolis into the hydrophobic core of ${\gamma}$-cyclodextrin. The incorporation efficiency of propolis in the nanoparticles was $50{\pm}2.3%$. Propolis nanoparticles observed by a scanning electron microscope (SEM) were spherical with the size of 30~40 nm. The swelling behaviors of the hydrogel film containing propolis nanoparticles showed a similar pattern with the hydrogel film without propolis nanoparticles. The cumulative amount of propolis released from the hydrogel film containing propolis nanoparticles in the buffer of pH 7.4 and 5.5 was $86.0{\pm}2.0%$ and $64.6{\pm}1.0%$ of total propolis loaded in the hydrogel film within 9 h, respectively. These results provide a rationale for studying wound healing application of the hydrogel film containing propolis nanoparticles in a clinical setting.

• 동의생리병리학회지
• /
• 제34권1호
• /
• pp.1-6
• /
• 2020

Arctii Lappa Fructus has the numerous health benefits, including antioxidant, anti-inflammatory, and anti-carcinogenic properties. Skin lipids are one of several factors that maintain epidermal barrier function. This study was to explore the lipogenic effect by ethanol extract of Arctii Lappa Fructus (EAF) in sebocytes. First, it was confirmed that EAF exhibited high antioxidant activity and collagenase activity inhibition. We found that cholesterol and triglyceride levels of cells by EAF were increased significantly in a dose-dependent manner. Moreover, EAF increased the expression of transcription factor sterol regulatory element-binding protein-1 (SREBP-1) in the cells. These results suggest that EAF induces lipogenesis in cells through the activation of SREBP-1.

• Biomolecules & Therapeutics
• /
• 제18권3호
• /
• pp.316-320
• /
• 2010

Pruritus is one of major symptoms in atopic dermatis. The pathophysiological mechanism of pruritus is unclear. The search for pruritogen is important in elucidating the pathophysiological mechanism of pruritus in atopic dermatitis. Glucosylsphingosine (Gsp) is upregulated in the strateum corneum of atopic dermatitis patients. We investigated to determine whether Gsp induces itch-scratch responses (ISRs) in mice. Intradermal administration of Gsp induces ISRs. Gsp dose-dependently induced scratching response at 50-500 nmol/site range. Pretreatment with naltrexone, an opioid $\mu$ receptor antagonist, and capsaicin, a TrpV1 receptor agonist, inhibited Gsp-induced ISRs. Additionally, Gsp-induced ISRs were also suppressed by cyproheptadine, an antagonist of serotonin receptor. These findings suggest that Gsp-induced scratching might be at least partly mediated by capsaicin-sensitive primary afferents, and the opioids receptor systems might be involved in transmission of itch signaling in the central nervous system. Furthermore, our findings suggest that Gsp-induced ISRs may be attributable to the serotonin-mediated pathways and Gsp is not any more one of byproducts of abnormal skin barrier but can lead to induce pruritus, one of typical symptoms of atopic dermatitis.

• 대한설비공학회:학술대회논문집
• /
• 대한설비공학회 2006년도 하계학술발표대회 논문집
• /
• pp.953-958
• /
• 2006

Perimeter zone has been reinforced by active systems, such as fan-coil units, because it causes an increase in heating and cooling loads, dew condensation in winter, or discomfort with cold-draft to residents in buildings, through poor insulation by light-weighed skin due to progressing multi-storied buildings and skyscrapers. However, because these active systems raise Its capacity so that fossil fuel is used as much as they are added, and ultimately, greenhouse effect is urged, we proposed BIPV system functioned as solar collector which can substitute active system. As an early stage, heat balance equation in steady-state by Fortran was used not only for pre-heating effect and electric power capacity during the day in winter, but also for electric power capacity during day in slimmer and sky radiation effect during night in summer. Especially, we should have considered shading on PV, since even a little bit of it makes the efficiency too low for the PV to work. Still, when the flux of pre-heated air was increased to make air-barrier, its temperature was not enough to make it because the speed of heat exchange was too fast to warm up the air, thus the capacity to meet the condition was evaluated, and electric power from PV was made used for it.

• Child Health Nursing Research
• /
• 제25권2호
• /
• pp.112-122
• /
• 2019

Purpose: The purpose of this study was to investigate the prevalence of diaper dermatitis (DD), knowledge of DD prevention and treatment, and diaper hygiene practices among mothers with diaper-wearing children. Methods: The participants were 176 mothers who presented to an outpatient clinic at a children's hospital with diaper-wearing children. Data were collected using a structured self-administered questionnaire. Results: The percent of correct answer for knowledge about DD was 59.7%. Almost half of the participants' children had experienced at least 1 episode of DD during the last 6 months. Inappropriate diaper hygiene practices, such as using talcum powder on DD and rubbing with a dry towel after cleansing, were reported. Moreover, only 37% of mothers used the recommended skin barrier to prevent DD. Although many children suffer from DD, levels of educational experience and perceived need for education on this topic were low. Almost 70% of mothers obtained DD-related information through internet sites. Conclusion: Educating parents about the etiology of DD and evidence-based diaper hygiene practices is an important aspect of effective DD prevention and treatment. Internet sites or smartphone apps may be effective methods for education on DD prevention and treatment considering parents' preferences for ways to obtain health information.

• 대한화장품학회지
• /
• 제32권1호
• /
• pp.17-22
• /
• 2006

포스파티딜세린(Phosphatidylserine; PS)은 생체막에서 구조적인 역할을 담당하는 인지질로서, 생체 내 다양한 세포작용에 필수적인 신호전달 효소의 보조인자로서 작용하는 것으로 알려져 있다. 하지만, PS의 생리활성에 대한 연구는 거의 이루어지지 않았고, 특히 피부에서의 생리활성에 대한 연구는 전무한 실정이다. 본 연구에서는 무모생쥐의 피부에 tape-stripping으로 경표피수분손실(TEWL)의 증가를 유도한 후, PS를 도포함으로써 그 손실을 현저히 감소시켰다. 또한, PS 도포군의 피부에서 세라마이드 함량이 증가된 사실을 확인한 바 있다. PS 도포군에서 non-hydroxyl 세라마이드와 glucosyl 세라마이드의 함량이 비처리군과 비교하여 각각 1.4배와 1.6배로 증가하였다. PS는 또한 피부각질세포의 분화를 촉진하였다. 피부각질세포에 PS를 처리함으로써 세포 형태가 분화상을 띄고 있음을 현미경 상에서 확인하였고, 표피분화의 특이적 표지 단백질인 Involucrin (INV)과 Transglutaminase 1 (TG'ase 1)의 발현이 각각 3.5배와 3배로 현저히 증가하였음을 웨스턴 블랏을 통하여 확인하였다. 또한 무모생쥐 피부에 PS를 도포한 결과 INV와 loricrin 단백질 발현이 증가하였다. 본 연구는 PS가 피부에서 생리활성을 나타낸다는 최초의 증거를 제시하며, 구체적으로는 각질세포 분화를 촉진함으로써 피부 세라마이드 함량을 증가시키고 경표피 수분손실을 감소시켜 궁극적으로 피부장벽을 강화하는 작용을 한다는 것을 보여준다.

• Journal of Veterinary Science
• /
• 제24권3호
• /
• pp.44.1-44.17
• /
• 2023

Background: Antibiotic resistance is a significant public health concern around the globe. Antimicrobial peptides exhibit broad-spectrum and efficient antibacterial activity with an added advantage of low drug resistance. The higher water content and 3D network structure of the hydrogels are beneficial for maintaining antimicrobial peptide activity and help to prevent degradation. The antimicrobial peptide released from hydrogels also hasten the local wound healing by promoting epithelial tissue regeneration and granulation tissue formation. Objective: This study aimed at developing sodium alginate based hydrogel loaded with a novel antimicrobial peptide Chol-37(F34-R) and to investigate the characteristics in vitro and in vivo as an alternative antibacterial wound dressing to treat infectious wounds. Methods: Hydrogels were developed and optimized by varying the concentrations of crosslinkers and subjected to various characterization tests like cross-sectional morphology, swelling index, percent water contents, water retention ratio, drug release and antibacterial activity in vitro, and Pseudomonas aeruginosa infected wound mice model in vivo. Results: The results indicated that the hydrogel C proved superior in terms of cross-sectional morphology having uniformly sized interconnected pores, a good swelling index, with the capacity to retain a higher quantity of water. Furthermore, the optimized hydrogel has been found to exert a significant antimicrobial activity against bacteria and was also found to prevent bacterial infiltration into the wound site due to forming an impermeable barrier between the wound bed and external environment. The optimized hydrogel was found to significantly hasten skin regeneration in animal models when compared to other treatments in addition to strong inhibitory effect on the release of pro-inflammatory cytokines (interleukin-1β and tumor necrosis factor-α). Conclusions: Our results suggest that sodium alginate -based hydrogels loaded with Chol-37(F34-R) hold the potential to be used as an alternative to conventional antibiotics in treating infectious skin wounds.